FlyBase Allele Report: Scer\GAL4[ey.PB]

General Information

Symbol

Scer\GAL4^ey.PB

Species

S. cerevisiae

Name

eyeless promoter construct of Bonini

FlyBase ID

FBal0083446

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-ey.B}

Also Known As

ey-Gal4, eyeless-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Bonini et al., 1997)

Progenitor genotype

Carried in construct

P{GAL4-ey.B}

Cytology

Description

ey sequences regulate expression of a GAL4 driver.

(Bonini et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

eye disc

(Bonini et al., 1997)

Additional Information

Statement

Reference

In third instar larva, expressed in eye disc prior to furrow formation.

(Bonini et al., 1997)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2006)

abnormal size | adult stage, with N^DN.UAS.cUa

(Kenyon et al., 2003)

abnormal size | adult stage, with Ser^DN.UAS

(Kenyon et al., 2003)

abnormal size | adult stage, with Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2006)

abnormal size | larval stage, with N^DN.UAS.cUa

(Kenyon et al., 2003)

abnormal size | larval stage, with Ser^DN.UAS

(Kenyon et al., 2003)

decreased occurrence of cell division | larval stage, with N^DN.UAS.cUa

(Kenyon et al., 2003)

decreased occurrence of cell division | larval stage, with Ser^DN.UAS

(Kenyon et al., 2003)

lethal, with Atx2^UAS.cSa

(Satterfield et al., 2002)

lethal, with Mmus\Bax^UAS.cGa

(Gaumer et al., 2000)

partially lethal - majority die, with Hsap\MAPT^UAS.cWa

(Berger et al., 2006)

partially lethal - majority die, with Zzzz\CAG^{Q97.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2005)

partially lethal - majority die, with Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2006, Berger et al., 2006, Berger et al., 2005)

short lived, with Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2006)

viable, with Dr^UAS.cIa

(Mozer, 2001)

visible, with aop^UAS.cRa

(Kumar and Moses, 2001)

visible, with B-H1^UAS.cSa

(Kumar and Moses, 2001)

visible, with B-H2^UAS.cSa

(Kumar and Moses, 2001)

visible, with Dr^UAS.cIa

(Mozer, 2001)

visible, with RhoGAP93B^RNAi.UAS.cBa

(Billuart et al., 2001)

visible, with ttk^p69.UAS

(Kumar and Moses, 2001)

visible, with wg^UAS.cLa

(Kumar and Moses, 2001)

visible, with Xvas\XhoI^{UASp.Tag:Mito(Cs1),Tag:MYC}

(Xu et al., 2008)

visible, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Berger et al., 2005)

visible, with Zzzz\CAG^{Q97.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2005)

visible, with Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2006, Berger et al., 2005)

visible | adult stage, with Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2006)

visible | homeotic, with ci^wt.UAS

(Kumar and Moses, 2001)

visible | homeotic, with Delta^DN.UAS

(Kumar and Moses, 2001)

visible | homeotic, with mam^DN.UAS.cUa

(Kumar and Moses, 2001)

visible | homeotic, with N^DN.UAS.cUa

(Kumar and Moses, 2001)

visible | homeotic, with pnt^P1.UAS

(Kumar and Moses, 2001)

visible | homeotic, with Raf^CA.UAS.cUa

(Kumar and Moses, 2001)

visible | homeotic, with spi^s.UAS

(Kumar and Moses, 2001)

visible | homeotic | heat sensitive, with Ser^DN.UAS

(Kumar and Moses, 2001)

Phenotype Manifest In

adult head, with SoxN^K439.UAS

(Savare et al., 2005)

antenna | ectopic, with ci^wt.UAS

(Kumar and Moses, 2001)

antenna | ectopic, with Delta^DN.UAS

(Kumar and Moses, 2001)

antenna | ectopic, with mam^DN.UAS.cUa

(Kumar and Moses, 2001)

antenna | ectopic, with N^DN.UAS.cUa

(Kumar and Moses, 2001)

antenna | ectopic, with pnt^P1.UAS

(Kumar and Moses, 2001)

antenna | ectopic, with Raf^CA.UAS.cUa

(Kumar and Moses, 2001)

antenna | ectopic, with spi^s.UAS

(Kumar and Moses, 2001)

antenna | ectopic | heat sensitive, with Ser^DN.UAS

(Kumar and Moses, 2001)

antenna | increased number, with N^DN.UAS.cUa

(Kenyon et al., 2003)

antenna | increased number, with Ser^DN.UAS

(Kenyon et al., 2003)

antennal disc | ectopic, with N^DN.UAS.cUa

(Kumar and Moses, 2001)

eye, with aop^UAS.cRa

(Kumar and Moses, 2001)

eye, with B-H1^UAS.cSa

(Kumar and Moses, 2001)

eye, with B-H2^UAS.cSa

(Kumar and Moses, 2001)

eye, with ci^wt.UAS

(Kumar and Moses, 2001)

eye, with Delta^DN.UAS

(Kumar and Moses, 2001)

eye, with Dr^UAS.cIa

(Mozer, 2001)

eye, with Hsap\MAPT^R406W.UAS

(Berger et al., 2006)

eye, with Hsap\MAPT^UAS.cWa

(Berger et al., 2006)

eye, with mam^DN.UAS.cUa

(Kumar and Moses, 2001)

eye, with N^DN.UAS.cUa

(Kenyon et al., 2003, Kumar and Moses, 2001)

eye, with pnt^P1.UAS

(Kumar and Moses, 2001)

eye, with Raf^CA.UAS.cUa

(Kumar and Moses, 2001)

eye, with RhoGAP93B^RNAi.UAS.cBa

(Billuart et al., 2001)

eye, with Ser^DN.UAS

(Kenyon et al., 2003)

eye, with SoxN^UAS.cBa

(Savare et al., 2005)

eye, with spi^s.UAS

(Kumar and Moses, 2001)

eye, with sti³, sti^RNAi.Sym.UAS

(D'Avino et al., 2004)

eye, with sti^RNAi.Sym.UAS

(D'Avino et al., 2004)

eye, with ttk^p69.UAS

(Kumar and Moses, 2001)

eye, with wg^UAS.cLa

(Kumar and Moses, 2001)

eye, with Xvas\XhoI^{UASp.Tag:Mito(Cs1),Tag:MYC}

(Xu et al., 2008)

eye, with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Berger et al., 2005)

eye, with Zzzz\CAG^{Q97.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2005)

eye, with Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2006, Berger et al., 2006, Berger et al., 2005)

eye | adult stage, with Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2006)

eye | heat sensitive, with Ser^DN.UAS

(Kumar and Moses, 2001)

eye-antennal disc, with N^DN.UAS.cUa

(Kenyon et al., 2003)

eye-antennal disc, with Ser^DN.UAS

(Kenyon et al., 2003)

eye disc, with N^DN.UAS.cUa

(Kumar and Moses, 2001)

eye disc morphogenetic furrow, with Ser^DN.UAS

(Kumar and Moses, 2001)

head, with Atx2^UAS.cSa

(Satterfield et al., 2002)

nucleus, with sti^RNAi.Sym.UAS

(D'Avino et al., 2004)

ommatidium, with sti³, sti^RNAi.Sym.UAS

(D'Avino et al., 2004)

ommatidium, with sti^RNAi.Sym.UAS

(D'Avino et al., 2004)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)} has visible phenotype, enhanceable by Hsf[+]/Hsf¹

(Berger et al., 2006)

Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)} has abnormal size phenotype, enhanceable by Hsf[+]/Hsf¹

(Berger et al., 2006)

Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)} has short lived phenotype, enhanceable by Hsf[+]/Hsf¹

(Berger et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PB, Xvas\XhoI^{UASp.Tag:Mito(Cs1),Tag:MYC} has visible phenotype, suppressible by mt:CoI^A302T

(Xu et al., 2008)

Scer\GAL4^ey.PB, Xvas\XhoI^{UASp.Tag:Mito(Cs1),Tag:MYC} has visible phenotype, suppressible by mt:CoI^R301Q

(Xu et al., 2008)

Scer\GAL4^ey.PB, Xvas\XhoI^{UASp.Tag:Mito(Cs1),Tag:MYC} has visible phenotype, suppressible by mt:CoI^R301S

(Xu et al., 2008)

Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)} has visible phenotype, suppressible | partially by Scer\GAL4^ey.PB/pan^UAS.cWa

(Berger et al., 2005)

N^DN.UAS.cUa, Scer\GAL4^ey.PB has abnormal size | larval stage phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PB

(Kenyon et al., 2003)

N^DN.UAS.cUa, Scer\GAL4^ey.PB has decreased occurrence of cell division | larval stage phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PB

(Kenyon et al., 2003)

Suppressor of

Statement

Reference

Scer\GAL4^ey.PB/pan^UAS.cWa is a suppressor | partially of visible phenotype of Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)}

(Berger et al., 2005)

CycE^UAS.cLa, Scer\GAL4^ey.PB is a suppressor of abnormal size | larval stage phenotype of N^DN.UAS.cUa, Scer\GAL4^ey.PB

(Kenyon et al., 2003)

CycE^UAS.cLa, Scer\GAL4^ey.PB is a suppressor of decreased occurrence of cell division | larval stage phenotype of N^DN.UAS.cUa, Scer\GAL4^ey.PB

(Kenyon et al., 2003)

Scer\GAL4^ey.PB/Mmus\Eya2^UAS.cBa is a suppressor of visible | recessive phenotype of eya²

(Bui et al., 2000, Bonini et al., 1997)

Mmus\Eya1^UAS.cBa/Scer\GAL4^ey.PB is a suppressor of visible | recessive phenotype of eya²

(Bui et al., 2000)

Scer\GAL4^ey.PB/Mmus\Eya3^UAS.cBa is a suppressor of visible | recessive phenotype of eya²

(Bui et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.PB, Zzzz\Poly-Ala^{A37.UAS.EGFP,Tag:NLS(SV40-largeT)} has eye phenotype, enhanceable by Hsf[+]/Hsf¹

(Berger et al., 2006)

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has eye phenotype, enhanceable by Rho1^72O/Rho1[+]

(D'Avino et al., 2004)

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has ommatidium phenotype, enhanceable by Rho1^72O/Rho1[+]

(D'Avino et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PB, Xvas\XhoI^{UASp.Tag:Mito(Cs1),Tag:MYC} has eye phenotype, suppressible by mt:CoI^A302T

(Xu et al., 2008)

Scer\GAL4^ey.PB, Xvas\XhoI^{UASp.Tag:Mito(Cs1),Tag:MYC} has eye phenotype, suppressible by mt:CoI^R301Q

(Xu et al., 2008)

Scer\GAL4^ey.PB, Xvas\XhoI^{UASp.Tag:Mito(Cs1),Tag:MYC} has eye phenotype, suppressible by mt:CoI^R301S

(Xu et al., 2008)

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has eye phenotype, suppressible by Rac1^J11/Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1[+]

(D'Avino et al., 2004)

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has ommatidium phenotype, suppressible by Rac1^J11/Rac2^Δ/Mtl[+]/Mtl^Δ/Rac2[+]/Rac1[+]

(D'Avino et al., 2004)

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has eye phenotype, suppressible by sqh^E20.E21

(D'Avino et al., 2004)

Scer\GAL4^ey.PB, sti^RNAi.Sym.UAS has ommatidium phenotype, suppressible by sqh^E20.E21

(D'Avino et al., 2004)

N^DN.UAS.cUa, Scer\GAL4^ey.PB has eye disc phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PB

(Kenyon et al., 2003)

NOT Enhancer of

Statement

Reference

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-enhancer of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-enhancer of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-enhancer of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-enhancer of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-enhancer of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-enhancer of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-enhancer of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-enhancer of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-enhancer of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-enhancer of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-enhancer of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Suppressor of

Statement

Reference

Scer\GAL4^ey.PB/sens^UAS.cNa is a suppressor of eye phenotype of Egfr^E1

(Frankfort and Mardon, 2004)

Scer\GAL4^ey.PB/sens^UAS.cNa is a suppressor of ommatidium phenotype of Egfr^E1

(Frankfort and Mardon, 2004)

CycE^UAS.cLa, Scer\GAL4^ey.PB is a suppressor of eye disc phenotype of N^DN.UAS.cUa, Scer\GAL4^ey.PB

(Kenyon et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, eya^UAS.cPa is a suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, eya^UAS.cPa is a suppressor of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Mmus\Eya1^UAS.cBa/Scer\GAL4^ey.PB is a suppressor of eye phenotype of eya²

(Bui et al., 2000)

Scer\GAL4^ey.PB/Mmus\Eya2^UAS.cBa is a suppressor of eye phenotype of eya²

(Bui et al., 2000, Bonini et al., 1997)

Scer\GAL4^ey.PB/Mmus\Eya3^UAS.cBa is a suppressor of eye phenotype of eya²

(Bui et al., 2000)

NOT Suppressor of

Statement

Reference

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-suppressor of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa/Scer\GAL4^ey.PB is a non-suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-suppressor of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB/eya^UAS.cPa is a non-suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

dpp^UAS.cSa, Scer\GAL4^ey.PB, so^UAS.cPa is a non-suppressor of photoreceptor neuron | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-suppressor of eye disc | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Scer\GAL4^ey.PB, so^UAS.cPa, eya^UAS.cPa is a non-suppressor of eye disc morphogenetic furrow | somatic clone phenotype of smo^D16

(Pappu et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ey.PB/eya^UAS.cBa rescues eya²

(Bui et al., 2000)

Partially rescues

Scer\GAL4^ey.PB/para^13.5.UAS partially rescues para^Ocd-1

(Lindsay et al., 2008)

Scer\GAL4^ey.PB/eya^UAS.cHa partially rescues eya²

(Rayapureddi et al., 2003)

eya^D493N.UAS/Scer\GAL4^ey.PB partially rescues eya²

(Rayapureddi et al., 2003)

Scer\GAL4^ey.PB/eya^E7.UAS partially rescues eya²

(Bui et al., 2000)

Scer\GAL4^ey.PB/eya^E11.UAS partially rescues eya²

(Bui et al., 2000)

Scer\GAL4^ey.PB/eya^UAS.cBa partially rescues eya²

(Bonini et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^ey.PB

Scer\GAL4^ey.PB

Name Synonyms

eyeless promoter construct of Bonini

(Bonini et al., 1997)

Secondary FlyBase IDs

References (20)

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