the Mushroom Body (MB) γ neurons retain their larval characteristics including both dorsal and medial lobes and a prominent calyx. Gross abnormalities in brain development are also seen.
animals survive to the third larval instar stage, but they do not pupariate. They initiate wandering, but fail to evert spiracles, shorten, attach themselves to a solid surface, or harden their cuticle. The larvae easily slip free of their cuticle when dissected, indicating that apolysis of the larval cuticle has been completed. The imaginal cells of the midgut islands begin to proliferate in EcRW53st
larvae, but they fail to form a tube surrounding the larval epithelial cells, and the epithelial cells fail to become condensed into a compact mass. Proliferation of abdominal histoblasts is initiated in these larvae, but is limited to one or two cell doublings.