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General Information
Symbol
Dmel\EcRW53st
Species
D. melanogaster
Name
FlyBase ID
FBal0083483
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
G6123403A
Reported nucleotide change:
G?A
Amino acid change:
W53term | EcR-PB; W53term | EcR-PG
Reported amino acid change:
W53term
Comment:
TGG to TAG nonsense mutation at codon W53.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Mutation is within the EcR-B1 specific exon 2.
Nucleotide substitution: G?A.
Amino acid replacement: W53term.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
In EcRW53st/EcR31 the Mushroom Body (MB) γ neurons retain their larval characteristics including both dorsal and medial lobes and a prominent calyx. Gross abnormalities in brain development are also seen.
Some EcRW53st/EcRM554fs animals survive to the third larval instar stage, but they do not pupariate. They initiate wandering, but fail to evert spiracles, shorten, attach themselves to a solid surface, or harden their cuticle. The larvae easily slip free of their cuticle when dissected, indicating that apolysis of the larval cuticle has been completed. The imaginal cells of the midgut islands begin to proliferate in EcRW53st/EcRM554fs larvae, but they fail to form a tube surrounding the larval epithelial cells, and the epithelial cells fail to become condensed into a compact mass. Proliferation of abdominal histoblasts is initiated in these larvae, but is limited to one or two cell doublings.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
EcRW53st/EcR[+] is a suppressor of visible phenotype of PaxUAS.cCa, Scer\GAL432B
Phenotype Manifest In
Suppressor of
Statement
Reference
EcRW53st/EcR[+] is a suppressor of wing phenotype of PaxUAS.cCa, Scer\GAL432B
Additional Comments
Genetic Interactions
Statement
Reference
One copy of EcRW53st significantly suppresses the Scer\GAL432B-driven PaxScer\UAS.cCa-induced wing blistering.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)