Allele Dmel\EcR^W53st

General Information
Symbol	Dmel\EcR^W53st	Species	D. melanogaster
Name		FlyBase ID	FBal0083483
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\EcR

Allele class
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Bender et al., 1997)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: W53. W53 falls within the EcR-B1 specific exon 2. Nucleotide substitution: G?A. (Bender et al., 1997)
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Bender et al., 1997) lethal \| embryonic stage (with EcR¹²⁰) (Carney et al., 2004) semi-lethal \| pupal stage (with EcR¹¹) (Carney et al., 2004)
Phenotype Manifest In
	midgut imaginal island (Bender et al., 1997) larval midgut & embryo (Bender et al., 1997) histoblast nest (Bender et al., 1997) mushroom body (with EcR³¹) (Lee et al., 2000) gamma-lobe & neuron (with EcR³¹) (Lee et al., 2000) adult brain (with EcR³¹) (Lee et al., 2000)
Detailed Description
	Statement Reference Some EcR^W53st/EcR^M554fs animals survive to the third larval instar stage, but they do not pupariate. They initiate wandering, but fail to evert spiracles, shorten, attach themselves to a solid surface, or harden their cuticle. The larvae easily slip free of their cuticle when dissected, indicating that apolysis of the larval cuticle has been completed. The imaginal cells of the midgut islands begin to proliferate in EcR^W53st/EcR^M554fs larvae, but they fail to form a tube surrounding the larval epithelial cells, and the epithelial cells fail to become condensed into a compact mass. Proliferation of abdominal histoblasts is initiated in these larvae, but is limited to one or two cell doublings. (Bender et al., 1997) In EcR^W53st/EcR³¹ the Mushroom Body (MB) γ neurons retain their larval characteristics including both dorsal and medial lobes and a prominent calyx. Gross abnormalities in brain development are also seen. (Lee et al., 2000)
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	EcR¹¹ (Carney et al., 2004)
Fails to complement	EcR¹²⁰ (Carney et al., 2004)
Rescued by	EcR³¹/EcR^W53st is rescued by Scer\GAL4^Tab2-201Y/EcR^B1.Scer\UAS (Lee et al., 2000) EcR³¹/EcR^W53st is rescued by Scer\GAL4^Tab2-201Y/EcR^B2.Scer\UAS (Lee et al., 2000)
Not rescued by	EcR³¹/EcR^W53st is not rescued by Scer\GAL4^Tab2-201Y/EcR^A.Scer\UAS (Lee et al., 2000)
Comments
Stocks ( 2 )
Kyoto	108328 EcR^W53st/SM6a
Bloomington	5604 cn¹ EcR^W53st bw¹/SM6b, bw^*
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	EcR^w53st (Biyasheva et al., 2001) EcR^W53St (Lee et al., 2000, Schubiger et al., 1998) EcR^W53st
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Davis et al., 2005, Dev. Biol. 282(2): 385--396 Phenotypic analysis of EcR-A mutants suggests that EcR isoforms have unique functions during Drosophila development. [FBrf0187381] Carney et al., 2004, Molec. Genet. Genomics 271(3): 282--290 Creation of EcR isoform-specific mutations in Drosophila melanogaster via local P element transposition, imprecise P element excision, and male recombination. [FBrf0174972] Cherbas et al., 2003, Development 130(2): 271--284 EcR isoforms in Drosophila: testing tissue-specific requirements by targeted blockade and rescue. [FBrf0155708] Chen et al., 2002, Mech. Dev. 114(1-2): 95--107 Induction of the early-late Ddc gene during Drosophila metamorphosis by the ecdysone receptor. [FBrf0147144] Biyasheva et al., 2001, Dev. Biol. 231(1): 234--251 Glue secretion in the Drosophila salivary gland: a model for steroid-regulated exocytosis. [FBrf0134544] Lee et al., 2000, Neuron 28(3): 807--818 Cell-autonomous requirement of the USP/EcR-B ecdysone receptor for mushroom body neuronal remodeling in Drosophila. [FBrf0132434] Schubiger et al., 1998, Development 125(11): 2053--2062 Drosophila EcR-B ecdysone receptor isoforms are required for larval molting and for neuron remodeling during metamorphosis. [FBrf0102730] Bender et al., 1997, Cell 91(6): 777--788 Drosophila ecdysone receptor mutations reveal functional differences among receptor isoforms. [FBrf0099891]

Allele Dmel\EcRW53st

Allele Dmel\EcR^W53st