Allele Dmel\Cam^3c1

General Information
Symbol	Dmel\Cam^3c1	Species	D. melanogaster
Name		FlyBase ID	FBal0083600
Feature type	allele	Associated gene	Dmel\Cam

Map ( GBrowse )
Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Arredondo et al., 1998, Harrisingh et al., 2007)
Mutagen	ethyl methanesulfonate (Nelson et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:8,152,338..8,152,338 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=E32K\|Cam-PB,E32K\|Cam-PA reported_pr_change=E31K na_change=G8152338A (Arredondo et al., 1998)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: E31K. (Arredondo et al., 1998) Amino acid replacement: E31K. FlyBase curator comment: The Figure shows lesion as an E to K change. The Figure Legend incorrectly reports a Q to K change. (Nelson et al., 1997)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective (Nelson et al., 1997, Arredondo et al., 1998) flight defective (Arredondo et al., 1998) flightless (Nelson et al., 1997) lethal \| recessive (Nelson et al., 1997) locomotor behavior defective (Arredondo et al., 1998) neuroanatomy defective (with Camⁿ³³⁹) (Arredondo et al., 1998) uncoordinated (with Camⁿ³³⁹) (Arredondo et al., 1998)
Phenotype Manifest In
	bouton & abdominal 2 ventral longitudinal muscle 2 (Arredondo et al., 1998) bouton & abdominal 3 ventral longitudinal muscle 2 (Arredondo et al., 1998) bouton & abdominal 4 ventral longitudinal muscle 2 (Arredondo et al., 1998) bouton & abdominal 5 ventral longitudinal muscle 2 (Arredondo et al., 1998) bouton & abdominal 6 ventral longitudinal muscle 2 (Arredondo et al., 1998) bouton & abdominal 7 ventral longitudinal muscle 2 (Arredondo et al., 1998) mesothoracic tergum (Nelson et al., 1997) wing vein \| ectopic (Nelson et al., 1997)
Detailed Description
	Statement Reference Cam^3c1 heterozygotes exhibit little effect on the average period of free-running. (Harrisingh et al., 2007) Cam^3c1/Camⁿ³³⁹ flies exhibit reduced locomotion, coordination and flight ability. A slight dominant effect can be detected for both Cam^3c1 and Camⁿ³³⁹. The recessive mutant effects are more substantial. In the larval neuromuscular junction voltage clamp preparation, working on muscle 6, ejc amplitude for Cam^3c1/Camⁿ³³⁹ is normal. Ejc amplitude is increased approximately three fold by quinidine at low external calcium concentrations (at 0.4mM external calcium quinidine has no effect. The mejc amplitude and frequency is unchanged, suggesting the increase in ejc reflects increased neurotransmitter release. Cam^3c1/Camⁿ³³⁹ larvae show structural synaptic abnormalities: the terminal arbor forms a thickened, or large, misshapen structure with few distinct boutons. This results in a reduced number of boutons and a nearly complete lack of terminal branching in pleural external longitudinal muscle 13. Muscle 13 shows the same ejc phenotype as ventral internal longitudinal muscle 6. No abnormalities in nerve terminals on muscles ventral internal longitudinal muscle 6, 7 or pleural external longitudinal muscle 12 have been observed. Cam^3c1/Cam^3c1 homozygous larvae show normal synaptic transmission, even in the presence of quinidine, and normal structure of synaptic boutons. (Arredondo et al., 1998) Electrophysiological and physiological defects. Mutation has a detectable maternal effect on egg hatch rate. Mutation may also interfere with male mating behaviour. Transheterozygous combinations with other Cam mutations produces an incompletely penetrant ectopic wing vein phenotype and either lateral or bilateral cuticular 'scabs' on the notum. (Nelson et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Cam^3c1, l(2)C43^C43 has viable \| reduced phenotype (Nelson et al., 1997, Nelson et al., 1997)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference In combination with l(2)C43^C43 mutants exhibit markedly reduced viability. (Nelson et al., 1997)
Xenogenetic Interactions
Statement Reference A heterozygous Cam^3c1 background in flies expressing Mmmm\PV^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^P2.4.Pdf has no effect on the length of the free-running period, compared to controls, after 1-5 weeks in constant darkness. (Harrisingh et al., 2007)
Complementation & Rescue Data
Partially complements	Cam^4c1 (Nelson et al., 1997) Cam⁵ (Nelson et al., 1997) Cam^6c4 (Nelson et al., 1997) Cam⁷ (Nelson et al., 1997) Cam^8t (Nelson et al., 1997) Camⁿ³³⁹ (Nelson et al., 1997)
Rescued by	Cam^3c1/Camⁿ³³⁹ is rescued by Cam^hs.PA (Arredondo et al., 1998) Cam^3c1 is rescued by Cam^hs.PN (Nelson et al., 1997)
Comments	Defects can be completely rescued by expression of Cam^hs.PN. (Nelson et al., 1997)
Stocks ( 1 )
Bloomington	6811 Cam^3c1 L²/CyO, amos^Roi-1 bw¹
Notes on Origin
Discoverer

Comments
	The Cam^3c1 mutation might effect Ca²⁺ buffering or interfere with the activation or inhibition of a calmodulin target distinct from that encoded by CaMKII or calmodulin activated adenylyl cyclase, rut. (Arredondo et al., 1998) Allelic series of the relative strength of the lethal mutations: Cam^8t > Cam⁷ > Cam^6c4 > Cam⁵ > Cam^4c1 > Cam^3c1. (Nelson et al., 1997)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Cam^3c1 (Harrisingh et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Harrisingh et al., 2007, J. Neurosci. 27(46): 12489--12499 Intracellular Ca[2+] Regulates Free-Running Circadian Clock Oscillation In Vivo. [FBrf0204998] Arredondo et al., 1998, Genetics 150(1): 265--274 Increased transmitter release and aberrant synapse morphology in a Drosophila calmodulin mutant. [FBrf0104398] Nelson et al., 1997, Genetics 147(4): 1783--1798 Calmodulin point mutations affect Drosophila development and behavior. [FBrf0099767]

Allele Dmel\Cam3c1

Allele Dmel\Cam^3c1