Allele Dmel\sqh^{A21.T:Zzzz\FLAG}

General Information
Symbol	Dmel\sqh^{A21.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0083733
Feature type	allele	Associated gene	Dmel\sqh

Allele class
Mutagen	in vitro construct - site directed mutagenesis, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Jordan and Karess, 1997) in vitro construct - site directed mutagenesis (Jordan and Karess, 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: S21A. Construct: Contains sqh, expressed under its own promoter and containing the mutation described above, tagged with T:Zzzz\FLAG. (Jordan and Karess, 1997)
Carried in construct	P{sqh-A21} (Jordan and Karess, 1997, Billuart et al., 2001, Roth et al., 1999, Vereshchagina et al., 2004, Lee and Treisman, 2004, Winter et al., 2001)
Tagged with	T:Zzzz\FLAG (Jordan and Karess, 1997, Roth et al., 1999, Winter et al., 2001, Billuart et al., 2001, Lee and Treisman, 2004, Vereshchagina et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	cytokinesis defective (Roth et al., 1999)
Phenotype Manifest In
	cephalopharyngeal skeleton \| germline clone (Roth et al., 1999) cystocyte \| germline clone (Roth et al., 1999) denticle belt \| germline clone (Roth et al., 1999) dorsal appendage \| germline clone (Roth et al., 1999) egg \| germline clone (Roth et al., 1999) egg chamber \| germline clone (Roth et al., 1999) embryonic segment \| germline clone (Roth et al., 1999) nurse cell \| germline clone (Roth et al., 1999) oocyte \| germline clone (Roth et al., 1999)
Detailed Description
	Statement Reference One copy of sqh^{A21.T:Zzzz\FLAG} in a wild-type background has no effect on eye development. (Lee and Treisman, 2004) Approximately 10-20% of egg chambers in germline clones expressing sqh^{A21.T:Zzzz\FLAG} apparently have a failure of cytokinesis during the fourth round of cystocyte division as they consist of 7 binuclear nurse cells and one binuclear oocyte. A fraction of eggs derived from germline clones expressing sqh^{A21.T:Zzzz\FLAG} are deposited and have three or four dorsal appendages. The four appendages are found in two pairs separated by a variable fraction of the egg circumference. When three appendages are present, the central one often appears to be a fusion of two appendages. Eggs with three or four dorsal appendages can be fertilised and begin development. These embryos have posterior segmentation defects. Only 36% of these embryos show partial dorsalisation (reduced head skeleton and a narrowing of the denticle belts). (Roth et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference sqh^{A21.T:Zzzz\FLAG} is an enhancer of visible \| recessive phenotype of dsh¹ (Winter et al., 2001)
NOT Enhancer of
Statement Reference sqh^{A21.T:Zzzz\FLAG} is a non-enhancer of neuroanatomy defective phenotype of RhoGAPp190^{dsRNA.GAP.Scer\UAS}, Scer\GAL4^ey-OK107 (Billuart et al., 2001)
Suppressor of
Statement Reference sqh^{A21.T:Zzzz\FLAG} is a suppressor of lethal \| male phenotype of flw⁶ (Vereshchagina et al., 2004) sqh^{A21.T:Zzzz\FLAG} is a suppressor of lethal \| male phenotype of flw^G0172/flw^G0172 (Vereshchagina et al., 2004)
NOT Suppressor of
Statement Reference sqh^{A21.T:Zzzz\FLAG} is a non-suppressor of lethal \| recessive \| larval stage phenotype of rok² (Winter et al., 2001) sqh^{A21.T:Zzzz\FLAG} is a non-suppressor of neuroanatomy defective phenotype of RhoGAPp190^{dsRNA.GAP.Scer\UAS}, Scer\GAL4^ey-OK107 (Billuart et al., 2001)
Phenotype Manifest In
Enhancer of
Statement Reference sqh^{A21.T:Zzzz\FLAG} is an enhancer of eye disc \| somatic clone phenotype of Mbs^T541 (Lee and Treisman, 2004) sqh^{A21.T:Zzzz\FLAG} is an enhancer of wing hair \| supernumerary phenotype of dsh¹ (Winter et al., 2001)
NOT Enhancer of
Statement Reference sqh^{A21.T:Zzzz\FLAG} is a non-enhancer of mushroom body vertical lobe phenotype of RhoGAPp190^{dsRNA.GAP.Scer\UAS}, Scer\GAL4^ey-OK107 (Billuart et al., 2001)
Suppressor of
Statement Reference sqh^{A21.T:Zzzz\FLAG} is a suppressor of eye disc \| somatic clone phenotype of Mbs^T541 (Lee and Treisman, 2004)
NOT Suppressor of
Statement Reference sqh^{A21.T:Zzzz\FLAG} is a non-suppressor of mushroom body vertical lobe phenotype of RhoGAPp190^{dsRNA.GAP.Scer\UAS}, Scer\GAL4^ey-OK107 (Billuart et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference Mbs^T541 photoreceptor clones are restored to their wild-type apical location in the eye disc when discs carry one copy of the constitutively inactive the sqh^{A21.T:Zzzz\FLAG} transgene. (Lee and Treisman, 2004) The viability of both flw^G0172 and flw⁶ mutants is much greater in a sqh^{A21.T:Zzzz\FLAG} background than a sqh^{A20.T:Zzzz\FLAG} background. (Vereshchagina et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	sqh^{A21.T:Zzzz\FLAG} partially rescues sqh^AX3 (Jordan and Karess, 1997)
Comments
Stocks ( 1 )
Bloomington	25765 y¹ w^*; P{sqh-A21}5
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	sqh^A21.T:FLAG sqh^{A21.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Lee and Treisman, 2004, Mol. Biol. Cell 15(7): 3285--3295 Excessive myosin activity in Mbs mutants causes photoreceptor movement out of the Drosophila eye disc epithelium. [FBrf0180500] Vereshchagina et al., 2004, Mol. Biol. Cell 15(10): 4395--4405 the essential role of PP1beta in Drosophila is to regulate nonmuscle myosin. [FBrf0180501] Billuart et al., 2001, Cell 107(2): 195--207 Regulating axon branch stability. The role of p190 RhoGAP in repressing a retraction signaling pathway. [FBrf0139610] Winter et al., 2001, Cell 105(1): 81--91 Drosophila Rho-associated kinase (Drok) links Frizzled-mediated planar cell polarity signaling to the actin cytoskeleton. [FBrf0135688] Roth et al., 1999, Development 126(5): 927--934 Binuclear Drosophila oocytes: consequences and implications for dorsal-ventral patterning in oogenesis and embryogenesis. [FBrf0107182] Jordan and Karess, 1997, J. Cell Biol. 139(7): 1805--1819 Myosin light chain-activating phosphorylation sites are required for oogenesis in Drosophila. [FBrf0100091]

Allele Dmel\sqhA21.T:Zzzz\FLAG

Allele Dmel\sqh^{A21.T:Zzzz\FLAG}