One copy of sqhA21.T:Zzzz\FLAG in a wild-type background has no effect on eye development.
Approximately 10-20% of egg chambers in germline clones expressing sqhA21.T:Zzzz\FLAG apparently have a failure of cytokinesis during the fourth round of cystocyte division as they consist of 7 binuclear nurse cells and one binuclear oocyte. A fraction of eggs derived from germline clones expressing sqhA21.T:Zzzz\FLAG are deposited and have three or four dorsal appendages. The four appendages are found in two pairs separated by a variable fraction of the egg circumference. When three appendages are present, the central one often appears to be a fusion of two appendages. Eggs with three or four dorsal appendages can be fertilised and begin development. These embryos have posterior segmentation defects. Only 36% of these embryos show partial dorsalisation (reduced head skeleton and a narrowing of the denticle belts).