Allele Dmel\slmb⁰⁰²⁹⁵

General Information
Symbol	Dmel\slmb⁰⁰²⁹⁵	Species	D. melanogaster
Name		FlyBase ID	FBal0083748
Feature type	allele	Associated gene	Dmel\slmb
Also Known As	slimb^P1493, slimb^P, l(3)00295

Allele class	loss of function allele
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Muzzopappa and Wappner, 2005)
Mutagen	P-element activity (Jiang and Struhl, 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} insertion 182 nucleotides upstream of the ATG. (Jiang and Struhl, 1998) Insertion of P{PZ} into the first exon of slmb. (Theodosiou et al., 1998)
Caused by insertion	P{PZ}slmb⁰⁰²⁹⁵ (Muzzopappa and Wappner, 2005, Jiang and Struhl, 1998, BDGP Project Members, 1994-1999, Watts et al., 2003, Noureddine et al., 2002, Theodosiou et al., 1998, Spradling et al., 1999, Roberts et al., 2012, Kuo et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death (Kuo et al., 2006) lethal (Jiang and Struhl, 1998) lethal \| embryonic stage \| recessive (Theodosiou et al., 1998) lethal \| pupal stage (Kuo et al., 2006) lethal \| recessive (Spradling et al., 1999) neuroanatomy defective (Kuo et al., 2006)
Phenotype Manifest In
	haltere (Jiang and Struhl, 1998) leg (Theodosiou et al., 1998) macrochaeta & wing margin (Jiang and Struhl, 1998) macrochaeta \| ectopic (Jiang and Struhl, 1998) ventral thoracic disc (Theodosiou et al., 1998) wing (Theodosiou et al., 1998) wing disc (Theodosiou et al., 1998)
Detailed Description
	Statement Reference slmb[00295] mutant C4da neuron clones exhibit an increase in cell death and die during early metamorphosis. (Kuo et al., 2006) No defects are seen in axon pruning during metamorphosis. (Watts et al., 2003) Homozygotes carrying a single copy of slmb^αTub84B.PJ are viable and show normal morphology, 5-10% of adults exhibit duplication of the haltere. Mutant cells undergo only a few divisions and show phenotypes characteristic of ectopic wg and hh signal transduction. Clones in the wing cause generation of ectopic sensory bristles along the wing margin, instead of epidermal hairs. Heterozygotes with slmb¹ die as pupae and heterozygotes with slmb² die during early larval life. (Jiang and Struhl, 1998) Clones of mutant cells in the leg and wing discs cause outgrowths of slmb⁺ cells and pattern disruptions including duplicated structures. Outgrowths occur both ventrally and dorsally and both anteriorly and posteriorly. (Theodosiou et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference ago³, slmb⁰⁰²⁹⁵ has increased cell number \| embryonic stage phenotype, suppressible by gcm²⁶ (Ho et al., 2009) ago³, slmb⁰⁰²⁹⁵ has increased cell number \| embryonic stage phenotype, suppressible by gcm^N7-4/gcm[+] (Ho et al., 2009) ago³, slmb⁰⁰²⁹⁵ has increased cell number \| embryonic stage phenotype, suppressible by slmb^{αTub84B.T:Hsap\MYC} (Ho et al., 2009)
Other
Statement Reference ago³, slmb⁰⁰²⁹⁵ has increased cell number \| embryonic stage phenotype (Ho et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference ago³, slmb⁰⁰²⁹⁵ has glial cell \| embryonic stage phenotype, suppressible by gcm²⁶ (Ho et al., 2009) ago³, slmb⁰⁰²⁹⁵ has glial cell \| embryonic stage phenotype, suppressible by gcm^N7-4/gcm[+] (Ho et al., 2009) ago³, slmb⁰⁰²⁹⁵ has glial cell \| embryonic stage phenotype, suppressible by slmb^{αTub84B.T:Hsap\MYC} (Ho et al., 2009)
Other
Statement Reference ago³, slmb⁰⁰²⁹⁵ has glial cell \| embryonic stage phenotype (Ho et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference slmb[00295], ago[3] double mutant embryos display a disrupted glial pattern compared to single mutant or control embryos. An increase in repo-positive glial cell number is observed. An increase in cell proliferation is observed, and no increase in cell death is seen. Expression of slmb[αTub84B.T:Hsap\MYC] in slmb[00295], ago[3] double mutant embryos significantly suppresses the increased number of glial cells observed in the slmb[00295], ago[3] mutant embryos. slmb[00295], ago[3], gcm[N7-4] triple mutants results in the absence of almost all glia, as observed in gcm[N7-4] single mutants. One copy of gcm[N7-4] suppresses the increased glial cell number phenotype observed in slmb[00295], ago[3] double mutants. gcm[26] suppresses the increased glial cell number phenotype observed in slmb[00295], ago[3] double mutants. (Ho et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	slmb⁰⁴²⁹⁵ (BDGP Project Members, 1994-1999) slmb⁰⁵⁴¹⁵ (BDGP Project Members, 1994-1999)
Rescued by	slmb⁰⁰²⁹⁵ is rescued by slmb^hs.PT (Theodosiou et al., 1998) slmb⁰⁰²⁹⁵ is rescued by slmb^αTub84B.PJ (Jiang and Struhl, 1998)
Comments
Stocks ( 2 )
Bloomington	11493 ry⁵⁰⁶ P{PZ}slmb⁰⁰²⁹⁵/TM3, ry^RK Sb¹ Ser¹
Kyoto	108870 ry⁵⁰⁶ P{PZ}slmb⁰⁰²⁹⁵/TM3, ry^RK Sb¹ Ser¹
Notes on Origin
Discoverer	A. Spradling.

Comments
	Wild type slmb function can be restored by precise excision of the P{PZ} insertion. (Jiang and Struhl, 1998) Complements: Atpα^01453a. Complements: ppan⁰²²³¹. Complements: Atpα⁰⁷⁰⁰⁸. Complements: rtet⁰⁷⁰⁸⁶. Complements: Atpα⁰⁷⁰⁹⁷. Complements: ppan^j6B6. Complements: l(3)neo54¹. (BDGP Project Members, 1994-1999) Previously assigned as an allele of Atpα in FlyBase (in error). Now known to be an allele of slmb, based on the insertion of the P{PZ} element within the slmb transcription unit, reversion of the mutant phenotype by precise excision of the P{PZ} insertion and rescue of the mutant phenotype by a transgene containing the slmb coding region (see FBrf0100581 and FBrf0104785).
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	00295 (Watts et al., 2003) Atpα⁰⁰²⁹⁵ l(3)00295 (Spradling et al., 1999, Jiang and Struhl, 1998, ) slimb^P1493 (Noureddine et al., 2002, Takacs et al., 2008, Ho et al., 2009) slimb^P (Ou et al., 2002, Wang et al., 1999) slmb⁰⁰²⁹⁵ (Christensen et al., 2008.4.15) slmb^P1493 slmb^PZ00295 (Theodosiou et al., 1998)
Name Synonym
Secondary FlyBase IDs
	FBal0000794
References ( 16 )
Research paper	Roberts et al., 2012, PLoS ONE 7(2): e31284 Defining Components of the ßcatenin Destruction Complex and Exploring Its Regulation and Mechanisms of Action during Development. [FBrf0217546] Ho et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(16): 6778--6783 Gcm protein degradation suppresses proliferation of glial progenitors. [FBrf0208044] Kuo et al., 2006, Neuron 51(3): 283--290 Identification of E2/E3 ubiquitinating enzymes and caspase activity regulating Drosophila sensory neuron dendrite pruning. [FBrf0195405] Muzzopappa and Wappner, 2005, Development 132(11): 2561--2571 Multiple roles of the F-box protein Slimb in Drosophila egg chamber development. [FBrf0187475] Watts et al., 2003, Neuron 38(6): 871--885 Axon pruning during Drosophila metamorphosis: evidence for local degeneration and requirement of the ubiquitin-proteasome system. [FBrf0161044] Noureddine et al., 2002, Dev. Cell 2(6): 757--770 Drosophila Roc1a encodes a RING-H2 protein with a unique function in processing the Hh signal transducer Ci by the SCF E3 ubiquitin ligase. [FBrf0148948] Ou et al., 2002, Genes Dev. 16(18): 2403--2414 Distinct protein degradation mechanisms mediated by Cul1 and Cul3 controlling Ci stability in Drosophila eye development. [FBrf0152002] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Wang et al., 1999, Genes Dev. 13(21): 2828--2837 Protein kinase A antagonizes Hedgehog signaling by regulating both the activator and repressor forms of cubitus interruptus. [FBrf0123234] Jiang and Struhl, 1998, Nature 391(6666): 493--496 Regulation of the Hedgehog and Wingless signalling pathways by the F-box/WD40-repeat protein Slimb. [FBrf0100581] Theodosiou et al., 1998, Development 125(17): 3411--3416 slimb coordinates wg and dpp expression in the dorsal-ventral and anterior-posterior axes during limb development. [FBrf0104785]
Supplementary material	Takacs et al., 2008, Science 319(5861): Supporting online material. [FBrf0202572]
Personal communication to FlyBase	Christensen et al., 2008.4.15, Isolation and characterization of Df(3R)BSC508. Isolation and characterization of Df(3R)BSC508. [FBrf0204568] Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\slmb00295

Allele Dmel\slmb⁰⁰²⁹⁵