FlyBase Allele Report: Dmel\RhoGEF2[4.1]

General Information

Symbol

Dmel\RhoGEF2^4.1

Species

D. melanogaster

Name

FlyBase ID

FBal0085930

Feature type

allele

Associated gene

Dmel\RhoGEF2

Associated Insertion(s)

Carried in Construct

Also Known As

DRhoGEF2^4.1

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Padash Barmchi et al., 2005, Nikolaidou, 2004)

Mutagen

ethyl methanesulfonate

(Barrett et al., 1997)

Progenitor genotype

Cytology

Description

Breakpoint between amino acids 763 and 885.

(Nikolaidou, 2004)

DNA rearrangement that disrupts the coding region.

(Barrett et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Embryos derived from RhoGEF2^4.1 germline clones contain a variable proportion of multinucleate cells.

(Großhans et al., 2005)

RhoGEF2^5.1/RhoGEF2^4.1 animals show 50% embryonic viability, 13% first larval instar viability and 0% adult viability. RhoGEF2^6.5/RhoGEF2^4.1 animals show 60% embryonic viability, 6% first larval instar viability and 0% adult viability. RhoGEF2^4.4/RhoGEF2^4.1 animals show 98% embryonic viability, 97% first larval instar viability and 0% adult viability. RhoGEF2^1.1/RhoGEF2^4.1 animals show 97% embryonic viability, 96% first larval instar viability and 0% adult viability. RhoGEF2^PX6/RhoGEF2^4.1 animals show 27% adult viability. 70% of the adults have wing defects; wings are crumpled and/or blistered. Wing discs of mid-third-instar larvae appear buckled, rather than having the normal pattern of folding. RhoGEF2^PX10/RhoGEF2^4.1 animals show 71% adult viability. 4% of the adults have wing defects. Salivary gland cells are not internalised in RhoGEF2^6.5/RhoGEF2^4.1 embryos, but remain on the surface (in contrast to wild-type embryos). Muscle and neuronal tissues show no significant defects in gross morphology in late-stage RhoGEF2^6.5/RhoGEF2^4.1 and RhoGEF2^5.1/RhoGEF2^4.1 embryos.

(Nikolaidou and Barrett, 2004)

The planar polarity of hairs in RhoGEF2^4.1/RhoGEF2^PX6 clones in the wing is normal. Ommatidial polarity is normal in homozygous clones in the eye.

(Nikolaidou, 2004)

Embryos derived from homozygous female germline clones have normal dorso-ventral and anterior-posterior patterning, and normal mesoderm specification. Germband extension and posterior midgut invagination appear defective. The cells of the mesectoderm fail to intercalate at the ventral midline. Gastrulation is highly disorganised and ventral furrow formation never occurs. Inappropriate lateral folds are formed. Invagination of the anterior midgut is also defective. Although cellularisation appears normal, nuclear migrations in the mesoderm precursors are not accompanied by obvious apical flattening, in contrast to wild-type, and the majority of mesodermal cells expand their apical surfaces rather than undergoing apical membrane constrictions at gastrulation.

(Barrett et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

RhoGEF2^PX10/RhoGEF2^4.1 has visible phenotype, enhanceable by cta⁵/cta[+]

(Nikolaidou and Barrett, 2004)

RhoGEF2^PX10/RhoGEF2^4.1 has partially lethal phenotype, enhanceable by cta⁵/cta[+]

(Nikolaidou and Barrett, 2004)

RhoGEF2^PX6/RhoGEF2^4.1 has visible phenotype, enhanceable by cta⁵/cta[+]

(Nikolaidou and Barrett, 2004)

RhoGEF2^PX6/RhoGEF2^4.1 has partially lethal phenotype, enhanceable by cta⁵/cta[+]

(Nikolaidou and Barrett, 2004)

Suppressor of

Statement

Reference

RhoGEF2^4.1/RhoGEF2[+] is a suppressor of abnormal neuroanatomy | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

RhoGEF2^4.1/RhoGEF2[+] is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

RhoGEF2^4.1 is a suppressor of visible phenotype of Rho1^GMR.PH

(Barrett et al., 1997)

Other

Statement

Reference

RhoGEF2^4.1, fog⁴/fog[+] has visible phenotype

(Nikolaidou, 2004, Nikolaidou and Barrett, 2004)

RhoGEF2^4.1/RhoGEF2[+], fog⁴ has visible | dominant phenotype

(Nikolaidou, 2004, Nikolaidou and Barrett, 2004)

Rho1^E3.10, RhoGEF2^4.1/RhoGEF2[+] has visible phenotype

(Bayer et al., 2003)

Rho1^12-6/Rho1[+], RhoGEF2^4.1 has visible phenotype

(Bayer et al., 2003)

RhoGEF2^4.1, Sb^sbd-1/Sb^sbd-201 has visible phenotype

(Bayer et al., 2003)

Rho1^J3.8, RhoGEF2^4.1/RhoGEF2[+] has visible phenotype

(Bayer et al., 2003)

RhoGEF2^4.1/RhoGEF2[+], zip^Ebr has visible phenotype

(Bayer et al., 2003)

RhoGEF2^4.1/RhoGEF2[+], Sb^sbd-1/Sb^sbd-201 has visible phenotype

(Bayer et al., 2003)

Rho1^12-6, RhoGEF2^4.1/RhoGEF2[+] has visible | dominant phenotype

(Bayer et al., 2003)

Rho1^E3.10/Rho1[+], RhoGEF2^4.1 has visible | dominant phenotype

(Bayer et al., 2003)

Rho1^J3.8/Rho1[+], RhoGEF2^4.1 has visible | dominant phenotype

(Bayer et al., 2003)

RhoGEF2^4.1, zip[+]/zip^Ebr has visible | dominant phenotype

(Bayer et al., 2003, Halsell et al., 2000)

RhoGEF2^4.1, zip^Ebr has visible | dominant phenotype

(Halsell et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

RhoGEF2^PX10/RhoGEF2^4.1 has wing phenotype, enhanceable by cta⁵/cta[+]

(Nikolaidou and Barrett, 2004)

RhoGEF2^PX6/RhoGEF2^4.1 has wing phenotype, enhanceable by cta⁵/cta[+]

(Nikolaidou and Barrett, 2004)

Suppressor of

Statement

Reference

RhoGEF2^4.1/RhoGEF2[+] is a suppressor of adult mushroom body | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

RhoGEF2^4.1/RhoGEF2[+] is a suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

RhoGEF2^4.1 is a suppressor of eye phenotype of Rho1^GMR.PH

(Barrett et al., 1997)

RhoGEF2^4.1 is a suppressor of embryonic head | dorsal phenotype of fog^hkb.PB

(Barrett et al., 1997)

Other

Statement

Reference

RhoGEF2^4.1, fog⁴ has wing phenotype

(Nikolaidou, 2004, Nikolaidou and Barrett, 2004)

RhoGEF2^4.1/RhoGEF2[+], fog⁴ has wing phenotype

(Nikolaidou, 2004, Nikolaidou and Barrett, 2004)

Rho1^E3.10, RhoGEF2^4.1 has wing phenotype

(Bayer et al., 2003)

RhoGEF2^4.1, Sb^sbd-1/Sb^sbd-201 has leg phenotype

(Bayer et al., 2003)

Rho1^E3.10, RhoGEF2^4.1 has leg phenotype

(Bayer et al., 2003)

Rho1^12-6, RhoGEF2^4.1 has leg phenotype

(Bayer et al., 2003)

RhoGEF2^4.1/RhoGEF2[+], Sb^sbd-1/Sb^sbd-201 has leg phenotype

(Bayer et al., 2003)

Rho1^12-6, RhoGEF2^4.1/RhoGEF2[+] has leg phenotype

(Bayer et al., 2003)

Rho1^J3.8, RhoGEF2^4.1 has leg phenotype

(Bayer et al., 2003)

Rho1^E3.10/Rho1[+], RhoGEF2^4.1 has wing phenotype

(Bayer et al., 2003)

Rho1^E3.10/Rho1[+], RhoGEF2^4.1 has leg phenotype

(Bayer et al., 2003)

Rho1^J3.8/Rho1[+], RhoGEF2^4.1 has leg phenotype

(Bayer et al., 2003)

RhoGEF2^4.1, zip[+]/zip^Ebr has leg phenotype

(Bayer et al., 2003, Halsell et al., 2000)

RhoGEF2^4.1, zip^Ebr has leg phenotype

(Bayer et al., 2003, Halsell et al., 2000)

RhoGEF2^4.1, zip[+]/zip^Ebr has wing phenotype

(Halsell et al., 2000)

RhoGEF2^4.1, zip[+]/zip^Ebr has femur phenotype

(Halsell et al., 2000)

RhoGEF2^4.1, zip[+]/zip^Ebr has tibia phenotype

(Halsell et al., 2000)

RhoGEF2^4.1, zip^Ebr has wing phenotype

(Halsell et al., 2000)

RhoGEF2^4.1, zip^Ebr has femur phenotype

(Halsell et al., 2000)

RhoGEF2^4.1, zip^Ebr has tibia phenotype

(Halsell et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

RhoGEF2^PX6/cta⁵ RhoGEF2^4.1 animals show 8% adult viability. The frequency of wing defects in these animals is 92% (compared to 70% in RhoGEF2^PX6/RhoGEF2^4.1 adults). RhoGEF2^PX10/cta⁵ RhoGEF2^4.1 animals show 28% adult viability. The frequency of wing defects in these animals is 35% (compared to 4% in RhoGEF2^PX10/RhoGEF2^4.1 adults). 6% of fog⁴/+ ; RhoGEF2^4.1/+ animals have wing defects.

(Nikolaidou and Barrett, 2004)

fog⁴/+ ; RhoGEF2^4.1/+ animals have wing defects.

(Nikolaidou, 2004)

RhoGEF2^4.1 shows a moderate interaction (25-49% of double heterozygotes have at least one malformed leg) with the following mutations: Rho1^12-6. br¹ fails to show a significant genetic interaction (assayed in terms of a malformed leg phenotype) with RhoGEF2^4.1. RhoGEF2^4.1 shows a moderate interaction (25-49% of double heterozygotes have at least one malformed wing) with the following mutations: Rho1^E3.10. Sb^sbd-201/Sb^sbd-1 shows a weak interaction (5-24% of double mutants have at least one malformed leg) with the following mutations: RhoGEF2^4.1/+.

(Bayer et al., 2003)

27% of RhoGEF2^4.1/zip^Ebr double heterozygotes have a malformed leg phenotype, which shows variable expressivity. In less severe cases, there is a dent in the femur or tibia. Flies with malformed legs often also show wing defects. Weak phenotypes include a broadening of the wing blade and folding back of the distal portion of the wing blade. In the most severe cases, wings are greatly reduced in size.

(Halsell et al., 2000)

Dominantly suppresses the Rho1^GMR.PH rough eye phenotype. The transient depression in the dorsal head region seen in embryos carrying fog^hkb.PB in a wild-type background is not seen in embryos carrying fog^hkb.PB which are derived from homozygous RhoGEF2^4.1 female germline clones.

(Barrett et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

RhoGEF2^11-3

(Bayer et al., 2003)

RhoGEF2⁰⁴²⁹¹

(Barrett et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9122

y¹ w^*; P{FRT(w^hs)}G13 RhoGEF2^4.1/CyO

Notes on Origin

Discoverer

Comments

Isolated as a dominant suppressor of the Rho1^GMR.PH rough eye phenotype.

(Barrett et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

4.1

(Nikolaidou, 2004)

DRhoGEF2^4.1

(Chang et al., 2016, Padash Barmchi et al., 2005, Nikolaidou and Barrett, 2004, Bayer et al., 2003, Asha et al., 1999, Barrett et al., 1997)

DrhoGEF^4.1

(Baer et al., 2007)

RhoGEF2^4.1

(Corrigall et al., 2007)

RhoGEF^4.1

(Ng and Luo, 2004)

Su(Rho1)2B^4.1

(Barrett et al., 1997)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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