FB2022_02, released March 29, 2022

Allele: Dmel\Ras85DV12.S35.UAS

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General Information
Symbol
Dmel\Ras85DV12.S35.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0085936
Feature type
allele
Associated gene
Dmel\Ras85D
Associated Insertion(s)
Carried in Construct
P{UAS-Ras85D.V12.S35}
Also Known As
UAS-RasV12S35, Ras85DV12S35, UAS-Ras185DV12S35
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - site directed mutagenesis
(Douziech et al., 2003, Karim and Rubin, 1998)
in vitro construct - amino acid replacement
(Karim and Rubin, 1998)
in vitro construct - regulatory fusion
(Douziech et al., 2003)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Ras85DV12.UAS
      (Karim and Rubin, 1998)
      Carried in construct
      P{UAS-Ras85D.V12.S35}
      Cytology
      Nature of the lesion
      Statement
      Reference

      Construct: Amino acid replacement: T35S.

      (Douziech et al., 2003)

      A mutation has been introduced into the effector loop (amino acid replacement T35S).

      (Karim and Rubin, 1998)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UAS
      UASt
      Encoded product / tool
      Ras85D
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cardiomyopathy
      CEA
      (Yu et al., 2013)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4esg-NP5130 in intestinal stem cell (ISC) somatic flip-out clones (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut results in massive clone overgrowth and highly increased ISC division.

      (Jin et al., 2015)

      Fly hearts expressing Scer\GAL4tin.CΔ4>Ras85DV12.S35.Scer\UAS have reduced end-diastolic dimensions compared with controls. Hearts isolated from the transgenic flies show abnormal cardiac morphology compared with controls. Histological analysis of cardiac chambers from adult transgenic flies show heart wall thickness that is similar to that of controls. The number of cardiomyocytes in the transgenic hearts is also similar to that of wild-type.

      (Yu et al., 2013)

      Expression of Ras85DV12.S35.Scer\UAS in the midgut, under the control of Scer\GAL4esg-NP7397 results in the generation of many new midgut cells, including enterocyte-like cells.

      (Jiang et al., 2011)

      Expression of Ras85DV12.S35.Scer\UAS in the motor neurons under the control of Scer\GAL4unspecified results in increased synaptic growth and increased transmitter release at the neuromuscular junction.

      Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel results in tumours in the eye.

      (Freeman et al., 2010)

      Expression of Ras85DV12.S35.Scer\UAS in differentiating eye cells driven by Scer\GAL4GMR.PU causes rough eyes, eye overgrowth and other abnormalities.

      (Yan et al., 2010)

      Ectopic expression of Ras85DV12.S35.Scer\UAS driven by Scer\GAL4esg-NP7397 in adult midgut progenitor cells results in their overproliferation compared with control third instar larval midguts.

      (Jiang and Edgar, 2009)

      Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4ey.PH early in the entire eye results in dramatic overgrowth and an increase in organ size.

      (Yan et al., 2009)

      Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4Cg.PA results in an increase in hemocyte numbers in larvae compared to controls.

      (Asha et al., 2003)

      Ras85DV12.S35.Scer\UAS; Scer\GAL4elav-C155 is lethal.

      (Botella et al., 2003)

      Flies expressing Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4sca.PU show the development of extra macrochaetae in the vicinity of the normal macrochaetae on the notum and scutellum.

      (Mirey et al., 2003)

      Caspase activation in cells posterior to the furrow in eye discs is completely suppressed by Ras85DV12.S35.Scer\UAS; Scer\GAL4GMR.PF. These discs also exhibit extensive ectopic photoreceptor differentiation posterior to the furrow.

      (Yang and Baker, 2003)

      Expression of Ras85DV12.S35.Scer\UAS, under the control of Scer\GAL4C380, induces a striking increase in the number of type I boutons at muscles 6 and 7 compared with controls expressing Ras85DScer\UAS.cKa and wild-type larvae. This increase is indistinguishable from the increase in bouton number observed in flies where Ras85DV12.Scer\UAS expression is driven by Scer\GAL4C380.

      (Koh et al., 2002)

      Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4Act5C.PP in wing disc cells results in an increase in cell size and an increase in clone area compared to controls. The proportion of cells in G1 is decreased compared to controls. Clones expressing Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4Act5C.PP are significantly more round than control clones.

      (Prober and Edgar, 2002)

      When expression is driven by Scer\GAL4sev.EP a rough eye phenotype results.

      (Therrien et al., 1999)

      Scer\GAL4dpp.blk1-mediated expression causes hyperplastic growth in third instar wing discs.

      (Karim and Rubin, 1998)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Ras85DV12.S35.UAS, Scer\GAL4GMR.PU has visible phenotype, enhanceable by Rabex-5VDRC.cUa, Scer\GAL4GMR.PU

      (Yan et al., 2010)
      NOT Enhanced by
      Statement
      Reference

      Ras85DV12.S35.UAS, Scer\GAL4sev.EP has visible phenotype, non-enhanceable by cnkhs.sev.Tag:FLAG

      (Therrien et al., 1999)
      Suppressed by
      NOT suppressed by
      Suppressor of
      Statement
      Reference

      Scer\GAL4c747/Ras85DV12.S35.UAS is a suppressor of abnormal learning phenotype of drkΔP24

      (Moressis et al., 2009)
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Ras85DV12.S35.UAS, Scer\GAL4GMR.PU has eye phenotype, enhanceable by Rabex-5VDRC.cUa, Scer\GAL4GMR.PU

      (Yan et al., 2010)
      Suppressed by
      NOT suppressed by
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut is partially suppressed by co-expression of either cicScer\UAS.T:Ivir\HA1 or cicΔC2.Scer\UAS.T:Ivir\HA1.

      The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing cicKK100838 under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut cannot be suppressed by co-expression of Ras85DdsRNA.Scer\UAS.cUa.

      (Jin et al., 2015)

      Co-expression of either kayFbz.Scer\UAS or CrebB-17AScer\UAS.b.cYa suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in larvae expressing Ras85DV12.S35.Scer\UAS in the motor neurons under the control of Scer\GAL4unspecified.

      Co-expression of either kayFbz.Scer\UAS or CrebB-17AScer\UAS.b.cYa does not suppress the eye tumour phenotype caused by expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel.

      (Freeman et al., 2010)

      MARCM clones of Ras85DV12.S35.Scer\UAS in Utx1 mosaic eyes result in mild overgrowth of the adult eye.

      (Herz et al., 2010)

      Co-expression of Rabex-5VDRC.cUa significantly enhances the number of eyes with black tissue and significantly increases lethality resulting from the expression of Ras85DV12.S35.Scer\UAS driven by Scer\GAL4GMR.PU. The overgrowth, extra antennae, and ectopic eye phenotypes of Ras85DV12.S35.Scer\UAS overexpression are also enhanced by Rabex-5VDRC.cUa-expression.

      Co-expression of Rabex-5Scer\UAS.T:Hsap\MYC driven by Scer\GAL4GMR.PU reduces the roughness, eye size, and black tissue phenotypes of Ras85DV12.S35.Scer\UAS-expressing eyes. The change in roughness is subtle.

      Co-expression of Rabex-5DPYT.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4GMR.PU reduces the roughness, eye size, and black tissue phenotypes of Ras85DV12.S35.Scer\UAS-expressing eyes. The change in roughness is subtle.

      (Yan et al., 2010)

      Expression of Ras85DV12.S35.Scer\UAS alone, or combined with the Scer\GAL4c747 driver but kept silent by Scer\GAL80ts.αTub84B does not suppress the learning deficit of drkΔP24/+ animals. In contrast, expression of Ras85DV12.S35.Scer\UAS by inactivation of the Scer\GAL80ts.αTub84B suppressor restores normal learning to drkΔP24 heterozygotes, while it does not appear to affect learning in control animals.

      (Moressis et al., 2009)

      The enhancement effect of Ras85DV12.G37.Scer\UAS on cnkhs.sev.T:Zzzz\FLAG contrasts sharply with the lack of enhancement of cnkhs.sev.T:Zzzz\FLAG by Ras85DV12.S35.Scer\UAS or Ras85DV12.C40.Scer\UAS.

      (Therrien et al., 1999)

      When expression is driven by Scer\GAL4hs.2sev, acts as a strong suppressor of the WGMR.PG eye phenotype.

      (Bergmann et al., 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in a plasmid and transfected into S2 cells.

      (Douziech et al., 2003)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Ras1V12S35
      (Koh et al., 2002)
      Ras85DV12.S35.Scer\UAS
      Ras85DV12.S35.UAS
      Ras85DV12S35
      (Douziech et al., 2003, Gaengel and Mlodzik, 2003)
      Name Synonyms
      Secondary FlyBase IDs
        References (20)