FlyBase Allele Report: Dmel\Ras85D[V12.S35.UAS]

Expression of Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^esg-NP5130 in intestinal stem cell (ISC) somatic flip-out clones (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut results in massive clone overgrowth and highly increased ISC division.

(Jin et al., 2015)

Fly hearts expressing Scer\GAL4^tin.CΔ4>Ras85D^{V12.S35.Scer\UAS} have reduced end-diastolic dimensions compared with controls. Hearts isolated from the transgenic flies show abnormal cardiac morphology compared with controls. Histological analysis of cardiac chambers from adult transgenic flies show heart wall thickness that is similar to that of controls. The number of cardiomyocytes in the transgenic hearts is also similar to that of wild-type.

(Yu et al., 2013)

Expression of Ras85D^{V12.S35.Scer\UAS} in the midgut, under the control of Scer\GAL4^esg-NP7397 results in the generation of many new midgut cells, including enterocyte-like cells.

(Jiang et al., 2011)

Expression of Ras85D^{V12.S35.Scer\UAS} in the motor neurons under the control of Scer\GAL4^unspecified results in increased synaptic growth and increased transmitter release at the neuromuscular junction.

Expression of Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^ey.3.5.Exel results in tumours in the eye.

(Freeman et al., 2010)

Expression of Ras85D^{V12.S35.Scer\UAS} in differentiating eye cells driven by Scer\GAL4^GMR.PU causes rough eyes, eye overgrowth and other abnormalities.

(Yan et al., 2010)

Ectopic expression of Ras85D^{V12.S35.Scer\UAS} driven by Scer\GAL4^esg-NP7397 in adult midgut progenitor cells results in their overproliferation compared with control third instar larval midguts.

(Jiang and Edgar, 2009)

Expression of Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^ey.PH early in the entire eye results in dramatic overgrowth and an increase in organ size.

(Yan et al., 2009)

Expression of Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^Cg.PA results in an increase in hemocyte numbers in larvae compared to controls.

(Asha et al., 2003)

Ras85D^{V12.S35.Scer\UAS}; Scer\GAL4^elav-C155 is lethal.

(Botella et al., 2003)

Flies expressing Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^sca.PU show the development of extra macrochaetae in the vicinity of the normal macrochaetae on the notum and scutellum.

(Mirey et al., 2003)

Caspase activation in cells posterior to the furrow in eye discs is completely suppressed by Ras85D^{V12.S35.Scer\UAS}; Scer\GAL4^GMR.PF. These discs also exhibit extensive ectopic photoreceptor differentiation posterior to the furrow.

(Yang and Baker, 2003)

Expression of Ras85D^{V12.S35.Scer\UAS}, under the control of Scer\GAL4^C380, induces a striking increase in the number of type I boutons at muscles 6 and 7 compared with controls expressing Ras85D^Scer\UAS.cKa and wild-type larvae. This increase is indistinguishable from the increase in bouton number observed in flies where Ras85D^V12.Scer\UAS expression is driven by Scer\GAL4^C380.

(Koh et al., 2002)

Expression of Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^Act5C.PP in wing disc cells results in an increase in cell size and an increase in clone area compared to controls. The proportion of cells in G₁ is decreased compared to controls. Clones expressing Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^Act5C.PP are significantly more round than control clones.

(Prober and Edgar, 2002)

When expression is driven by Scer\GAL4^sev.EP a rough eye phenotype results.

(Therrien et al., 1999)

Scer\GAL4^dpp.blk1-mediated expression causes hyperplastic growth in third instar wing discs.

(Karim and Rubin, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU

(Yan et al., 2010)

NOT Enhanced by

Statement

Reference

Ras85D^V12.S35.UAS, Scer\GAL4^sev.EP has visible phenotype, non-enhanceable by cnk^{hs.sev.Tag:FLAG}

(Therrien et al., 1999)

Suppressed by

Statement

Reference

Ras85D^V12.S35.UAS, Scer\GAL4^unspecified has neuroanatomy defective | larval stage phenotype, suppressible by CrebB^UAS.b.cYa, Scer\GAL4^unspecified

(Freeman et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^unspecified has neurophysiology defective | larval stage phenotype, suppressible by CrebB^UAS.b.cYa, Scer\GAL4^unspecified

(Freeman et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^unspecified has neuroanatomy defective | larval stage phenotype, suppressible by kay^Fbz.UAS, Scer\GAL4^unspecified

(Freeman et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^unspecified has neurophysiology defective | larval stage phenotype, suppressible by kay^Fbz.UAS, Scer\GAL4^unspecified

(Freeman et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU has visible phenotype, suppressible | partially by Rabex-5^UAS.Tag:MYC, Scer\GAL4^GMR.PU

(Yan et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU has visible phenotype, suppressible | partially by Rabex-5^{DPYT.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Yan et al., 2010)

NOT suppressed by

Statement

Reference

Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel has visible phenotype, non-suppressible by kay^Fbz.UAS, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel has visible phenotype, non-suppressible by CrebB^UAS.b.cYa, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^c747/Ras85D^V12.S35.UAS is a suppressor of learning defective phenotype of drk^ΔP24

(Moressis et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^c747, Scer\GAL80^ts.αTub84B, Ras85D^V12.S35.UAS is a non-suppressor of learning defective phenotype of drk^ΔP24

(Moressis et al., 2009)

Other

Statement

Reference

Ras85D^V12.S35.UAS, Scer\GAL4^Tub.PU, Utx¹ has size defective | somatic clone phenotype

(Herz et al., 2010)

Rabex-5^VDRC.cUa, Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU has lethal phenotype

(Yan et al., 2010)

Rala^S25N.cMa.UAS, Ras85D^V12.S35.UAS, Scer\GAL4^sca.PU has lethal | pupal stage phenotype

(Mirey et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU

(Yan et al., 2010)

Suppressed by

Statement

Reference

Ras85D^V12.S35.UAS, Scer\GAL4^NP5130 has adult midgut | somatic clone phenotype, suppressible | partially by cic^UAS.Tag:HA, Scer\GAL4^NP5130

(Jin et al., 2015)

Ras85D^V12.S35.UAS, Scer\GAL4^NP5130 has intestinal stem cell | somatic clone | adult stage phenotype, suppressible | partially by cic^UAS.Tag:HA, Scer\GAL4^NP5130

(Jin et al., 2015)

Ras85D^V12.S35.UAS, Scer\GAL4^NP5130 has adult midgut | somatic clone phenotype, suppressible | partially by cic^{ΔC2.UAS.Tag:HA}, Scer\GAL4^NP5130

(Jin et al., 2015)

Ras85D^V12.S35.UAS, Scer\GAL4^NP5130 has intestinal stem cell | somatic clone | adult stage phenotype, suppressible | partially by cic^{ΔC2.UAS.Tag:HA}, Scer\GAL4^NP5130

(Jin et al., 2015)

Ras85D^V12.S35.UAS, Scer\GAL4^unspecified has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by kay^Fbz.UAS, Scer\GAL4^unspecified

(Freeman et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^unspecified has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by CrebB^UAS.b.cYa, Scer\GAL4^unspecified

(Freeman et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by Rabex-5^UAS.Tag:MYC, Scer\GAL4^GMR.PU

(Yan et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by Rabex-5^{DPYT.UAS.Tag:MYC}, Scer\GAL4^GMR.PU

(Yan et al., 2010)

NOT suppressed by

Statement

Reference

Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel has eye phenotype, non-suppressible by kay^Fbz.UAS, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel has eye phenotype, non-suppressible by CrebB^UAS.b.cYa, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^hs.2sev/Ras85D^V12.S35.UAS is a suppressor of eye phenotype of hid^GMR.PG/W^GMR.PG

(Bergmann et al., 1998)

Scer\GAL4^hs.2sev/Ras85D^V12.S35.UAS is a suppressor of ommatidium phenotype of hid^GMR.PG/W^GMR.PG

(Bergmann et al., 1998)

Other

Statement

Reference

Ras85D^V12.S35.UAS, Scer\GAL4^Tub.PU, Utx¹ has eye | somatic clone phenotype

(Herz et al., 2010)

Rabex-5^VDRC.cUa, Ras85D^V12.S35.UAS, Scer\GAL4^GMR.PU has antenna phenotype

(Yan et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing btl::Egfr^{Scer\UAS.T:λ\cI-DD} under the control of Scer\GAL4^esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut is partially suppressed by co-expression of either cic^{Scer\UAS.T:Ivir\HA1} or cic^{ΔC2.Scer\UAS.T:Ivir\HA1}.

The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing cic^KK100838 under the control of Scer\GAL4^esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut cannot be suppressed by co-expression of Ras85D^{dsRNA.Scer\UAS.cUa}.

(Jin et al., 2015)

Co-expression of either kay^Fbz.Scer\UAS or CrebB-17A^{Scer\UAS.b.cYa} suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in larvae expressing Ras85D^{V12.S35.Scer\UAS} in the motor neurons under the control of Scer\GAL4^unspecified.

Co-expression of either kay^Fbz.Scer\UAS or CrebB-17A^{Scer\UAS.b.cYa} does not suppress the eye tumour phenotype caused by expression of Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^ey.3.5.Exel.

(Freeman et al., 2010)

MARCM clones of Ras85D^{V12.S35.Scer\UAS} in Utx¹ mosaic eyes result in mild overgrowth of the adult eye.

(Herz et al., 2010)

Co-expression of Rabex-5^VDRC.cUa significantly enhances the number of eyes with black tissue and significantly increases lethality resulting from the expression of Ras85D^{V12.S35.Scer\UAS} driven by Scer\GAL4^GMR.PU. The overgrowth, extra antennae, and ectopic eye phenotypes of Ras85D^{V12.S35.Scer\UAS} overexpression are also enhanced by Rabex-5^VDRC.cUa-expression.

Co-expression of Rabex-5^{Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PU reduces the roughness, eye size, and black tissue phenotypes of Ras85D^{V12.S35.Scer\UAS}-expressing eyes. The change in roughness is subtle.

Co-expression of Rabex-5^{DPYT.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^GMR.PU reduces the roughness, eye size, and black tissue phenotypes of Ras85D^{V12.S35.Scer\UAS}-expressing eyes. The change in roughness is subtle.

(Yan et al., 2010)

Expression of Ras85D^{V12.S35.Scer\UAS} alone, or combined with the Scer\GAL4^c747 driver but kept silent by Scer\GAL80^ts.αTub84B does not suppress the learning deficit of drk^ΔP24/+ animals. In contrast, expression of Ras85D^{V12.S35.Scer\UAS} by inactivation of the Scer\GAL80^ts.αTub84B suppressor restores normal learning to drk^ΔP24 heterozygotes, while it does not appear to affect learning in control animals.

(Moressis et al., 2009)

The enhancement effect of Ras85D^{V12.G37.Scer\UAS} on cnk^{hs.sev.T:Zzzz\FLAG} contrasts sharply with the lack of enhancement of cnk^{hs.sev.T:Zzzz\FLAG} by Ras85D^{V12.S35.Scer\UAS} or Ras85D^{V12.C40.Scer\UAS}.

(Therrien et al., 1999)

When expression is driven by Scer\GAL4^hs.2sev, acts as a strong suppressor of the W^GMR.PG eye phenotype.

(Bergmann et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2 cells.

(Douziech et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ras1V12S35

(Koh et al., 2002)

Ras85D^{V12.S35.Scer\UAS}

Ras85D^V12.S35.UAS

Ras85D^V12S35

(Douziech et al., 2003, Gaengel and Mlodzik, 2003)

Name Synonyms

Secondary FlyBase IDs

References (20)

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