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General Information
Symbol
Dmel\Ras85DV12.S35.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0085936
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-RasV12S35, Ras85DV12S35, UAS-Ras185DV12S35
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Construct: Amino acid replacement: T35S.

A mutation has been introduced into the effector loop (amino acid replacement T35S).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4esg-NP5130 in intestinal stem cell (ISC) somatic flip-out clones (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut results in massive clone overgrowth and highly increased ISC division.

Fly hearts expressing Scer\GAL4tin.CΔ4>Ras85DV12.S35.Scer\UAS have reduced end-diastolic dimensions compared with controls. Hearts isolated from the transgenic flies show abnormal cardiac morphology compared with controls. Histological analysis of cardiac chambers from adult transgenic flies show heart wall thickness that is similar to that of controls. The number of cardiomyocytes in the transgenic hearts is also similar to that of wild-type.

Expression of Ras85DV12.S35.Scer\UAS in the midgut, under the control of Scer\GAL4esg-NP7397 results in the generation of many new midgut cells, including enterocyte-like cells.

Expression of Ras85DV12.S35.Scer\UAS in the motor neurons under the control of Scer\GAL4unspecified results in increased synaptic growth and increased transmitter release at the neuromuscular junction.

Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel results in tumours in the eye.

Expression of Ras85DV12.S35.Scer\UAS in differentiating eye cells driven by Scer\GAL4GMR.PU causes rough eyes, eye overgrowth and other abnormalities.

Ectopic expression of Ras85DV12.S35.Scer\UAS driven by Scer\GAL4esg-NP7397 in adult midgut progenitor cells results in their overproliferation compared with control third instar larval midguts.

Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4ey.PH early in the entire eye results in dramatic overgrowth and an increase in organ size.

Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4Cg.PA results in an increase in hemocyte numbers in larvae compared to controls.

Flies expressing Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4sca.PU show the development of extra macrochaetae in the vicinity of the normal macrochaetae on the notum and scutellum.

Caspase activation in cells posterior to the furrow in eye discs is completely suppressed by Ras85DV12.S35.Scer\UAS; Scer\GAL4GMR.PF. These discs also exhibit extensive ectopic photoreceptor differentiation posterior to the furrow.

Expression of Ras85DV12.S35.Scer\UAS, under the control of Scer\GAL4C380, induces a striking increase in the number of type I boutons at muscles 6 and 7 compared with controls expressing Ras85DScer\UAS.cKa and wild-type larvae. This increase is indistinguishable from the increase in bouton number observed in flies where Ras85DV12.Scer\UAS expression is driven by Scer\GAL4C380.

Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4Act5C.PP in wing disc cells results in an increase in cell size and an increase in clone area compared to controls. The proportion of cells in G1 is decreased compared to controls. Clones expressing Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4Act5C.PP are significantly more round than control clones.

When expression is driven by Scer\GAL4sev.EP a rough eye phenotype results.

Scer\GAL4dpp.blk1-mediated expression causes hyperplastic growth in third instar wing discs.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut is partially suppressed by co-expression of either cicScer\UAS.T:Ivir\HA1 or cicΔC2.Scer\UAS.T:Ivir\HA1.

The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing cicKK100838 under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut cannot be suppressed by co-expression of Ras85DdsRNA.Scer\UAS.cUa.

Co-expression of either kayFbz.Scer\UAS or CrebB-17AScer\UAS.b.cYa suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in larvae expressing Ras85DV12.S35.Scer\UAS in the motor neurons under the control of Scer\GAL4unspecified.

Co-expression of either kayFbz.Scer\UAS or CrebB-17AScer\UAS.b.cYa does not suppress the eye tumour phenotype caused by expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel.

MARCM clones of Ras85DV12.S35.Scer\UAS in Utx1 mosaic eyes result in mild overgrowth of the adult eye.

Co-expression of Rabex-5VDRC.cUa significantly enhances the number of eyes with black tissue and significantly increases lethality resulting from the expression of Ras85DV12.S35.Scer\UAS driven by Scer\GAL4GMR.PU. The overgrowth, extra antennae, and ectopic eye phenotypes of Ras85DV12.S35.Scer\UAS overexpression are also enhanced by Rabex-5VDRC.cUa-expression.

Co-expression of Rabex-5Scer\UAS.T:Hsap\MYC driven by Scer\GAL4GMR.PU reduces the roughness, eye size, and black tissue phenotypes of Ras85DV12.S35.Scer\UAS-expressing eyes. The change in roughness is subtle.

Co-expression of Rabex-5DPYT.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4GMR.PU reduces the roughness, eye size, and black tissue phenotypes of Ras85DV12.S35.Scer\UAS-expressing eyes. The change in roughness is subtle.

Expression of Ras85DV12.S35.Scer\UAS alone, or combined with the Scer\GAL4c747 driver but kept silent by Scer\GAL80ts.αTub84B does not suppress the learning deficit of drkΔP24/+ animals. In contrast, expression of Ras85DV12.S35.Scer\UAS by inactivation of the Scer\GAL80ts.αTub84B suppressor restores normal learning to drkΔP24 heterozygotes, while it does not appear to affect learning in control animals.

The enhancement effect of Ras85DV12.G37.Scer\UAS on cnkhs.sev.T:Zzzz\FLAG contrasts sharply with the lack of enhancement of cnkhs.sev.T:Zzzz\FLAG by Ras85DV12.S35.Scer\UAS or Ras85DV12.C40.Scer\UAS.

When expression is driven by Scer\GAL4hs.2sev, acts as a strong suppressor of the WGMR.PG eye phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid and transfected into S2 cells.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Ras1V12S35
Ras85DV12.S35.Scer\UAS
Ras85DV12.S35.UAS
Name Synonyms
Secondary FlyBase IDs
    References (20)