Allele Dmel\Ras85D^{V12.S35.Scer\UAS}

General Information
Symbol	Dmel\Ras85D^{V12.S35.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0085936
Feature type	allele	Associated gene	Dmel\Ras85D
Also Known As	Ras85D^V12S35

Allele class
Mutagen	in vitro construct - site directed mutagenesis, in vitro construct - amino acid replacement, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Karim and Rubin, 1998) in vitro construct - regulatory fusion (Douziech et al., 2003) in vitro construct - site directed mutagenesis (Karim and Rubin, 1998, Douziech et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Ras85D^V12.Scer\UAS (Karim and Rubin, 1998)
Nature of the lesion	Statement Reference Construct: Amino acid replacement: T35S. (Douziech et al., 2003) Construct: Amino acid replacement: T35S. An effector loop mutant. (Karim and Rubin, 1998)
Carried in construct	P{UAS-Ras85D.V12.S35} (Botella et al., 2003, Asha et al., 2003, Yang and Baker, 2003, Karim and Rubin, 1998, Koh et al., 2002, Prober and Edgar, 2002, Gaengel and Mlodzik, 2003, Therrien et al., 1999, Bergmann et al., 1998, Mirey et al., 2003, Yan et al., 2009, Moressis et al., 2009, Yan et al., 2010, Jiang and Edgar, 2009, Herz et al., 2010, Freeman et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	hyperplasia, with Scer\GAL4^dpp.blk1 (Karim and Rubin, 1998) increased cell number, with Scer\GAL4^ey.PH (Yan et al., 2009) increased cell number \| third instar larval stage, with Scer\GAL4^esg-NP7397 (Jiang and Edgar, 2009) increased cell size \| somatic clone, with Scer\GAL4^Act5C.PP (Prober and Edgar, 2002) lethal, with Scer\GAL4^elav-C155 (Botella et al., 2003) neuroanatomy defective, with Scer\GAL4^futsch-C380 (Koh et al., 2002) neuroanatomy defective \| larval stage, with Scer\GAL4^unspecified (Freeman et al., 2010) neurophysiology defective \| larval stage, with Scer\GAL4^unspecified (Freeman et al., 2010) visible, with Scer\GAL4^ey.3.5.Exel (Freeman et al., 2010) visible, with Scer\GAL4^GMR.PU (Yan et al., 2010) visible, with Scer\GAL4^sca.PU (Mirey et al., 2003) visible, with Scer\GAL4^sev.EP (Therrien et al., 1999)
Phenotype Manifest In
	adult midgut precursor cell \| third instar larval stage, with Scer\GAL4^esg-NP7397 (Jiang and Edgar, 2009) embryonic/larval hemocyte \| supernumerary, with Scer\GAL4^Cg.PA (Asha et al., 2003) embryonic/larval neuromuscular junction \| larval stage, with Scer\GAL4^unspecified (Freeman et al., 2010) eye, with Scer\GAL4^ey.3.5.Exel (Freeman et al., 2010) eye, with Scer\GAL4^ey.PH (Yan et al., 2009) eye, with Scer\GAL4^GMR.PU (Yan et al., 2010) eye, with Scer\GAL4^sev.EP (Therrien et al., 1999) macrochaeta \| ectopic, with Scer\GAL4^sca.PU (Mirey et al., 2003) mitotic cell cycle \| somatic clone, with Scer\GAL4^Act5C.PP (Prober and Edgar, 2002) photoreceptor cell \| ectopic, with Scer\GAL4^GMR.PF (Yang and Baker, 2003) type I bouton \| supernumerary, with Scer\GAL4^futsch-C380 (Koh et al., 2002) wing disc, with Scer\GAL4^dpp.blk1 (Karim and Rubin, 1998)
Detailed Description
	Statement Reference Expression of Ras85D[V12.S35.Scer\UAS] in the motor neurons under the control of Scer\GAL4[unspecified] results in increased synaptic growth and increased transmitter release at the neuromuscular junction. Expression of Ras85D[V12.S35.Scer\UAS] under the control of Scer\GAL4[ey.3.5.Exel] results in tumours in the eye. (Freeman et al., 2010) Expression of Ras85D[V12.S35.Scer\UAS] in differentiating eye cells driven by Scer\GAL4[GMR.PU] causes rough eyes, eye overgrowth and other abnormalities. (Yan et al., 2010) Ectopic expression of Ras85D[V12.S35.Scer\UAS] driven by Scer\GAL4[esg-NP7397] in adult midgut progenitor cells results in their overproliferation compared with control third instar larval midguts. (Jiang and Edgar, 2009) Expression of Ras85D[V12.S35.Scer\UAS] under the control of Scer\GAL4[ey.PH] early in the entire eye results in dramatic overgrowth and an increase in organ size. (Yan et al., 2009) Expression of Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^Cg.PA results in an increase in hemocyte numbers in larvae compared to controls. (Asha et al., 2003) Ras85D^{V12.S35.Scer\UAS}; Scer\GAL4^elav-C155 is lethal. (Botella et al., 2003) Flies expressing Ras85D[V12.S35.Scer\UAS] under the control of Scer\GAL4[sca.PU] show the development of extra macrochaetae in the vicinity of the normal macrochaetae on the notum and scutellum. (Mirey et al., 2003) Caspase activation in cells posterior to the furrow in eye discs is completely suppressed by Ras85D^{V12.S35.Scer\UAS}; Scer\GAL4^GMR.PF. These discs also exhibit extensive ectopic photoreceptor differentiation posterior to the furrow. (Yang and Baker, 2003) Expression of Ras85D^{V12.S35.Scer\UAS}, under the control of Scer\GAL4^C380, induces a striking increase in the number of type I boutons at muscles 6 and 7 compared with controls expressing Ras85D^Scer\UAS.cKa and wild-type larvae. This increase is indistinguishable from the increase in bouton number observed in flies where Ras85D^V12.Scer\UAS expression is driven by Scer\GAL4^C380. (Koh et al., 2002) Expression of Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^Act5C.PP in wing disc cells results in an increase in cell size and an increase in clone area compared to controls. The proportion of cells in G₁ is decreased compared to controls. Clones expressing Ras85D^{V12.S35.Scer\UAS} under the control of Scer\GAL4^Act5C.PP are significantly more round than control clones. (Prober and Edgar, 2002) When expression is driven by Scer\GAL4^sev.EP a rough eye phenotype results. (Therrien et al., 1999) Scer\GAL4^dpp.blk1-mediated expression causes hyperplastic growth in third instar wing discs. (Karim and Rubin, 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU (Yan et al., 2010)
NOT Enhanced by
Statement Reference Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^sev.EP has visible phenotype, non-enhanceable by cnk^{hs.sev.T:Zzzz\FLAG} (Therrien et al., 1999)
Suppressed by
Statement Reference Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible \| partially by Rabex-5^{DPYT.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PU (Yan et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU has visible phenotype, suppressible \| partially by Rabex-5^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PU (Yan et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^unspecified has neuroanatomy defective \| larval stage phenotype, suppressible by CrebB^{Scer\UAS.b.cYa}, Scer\GAL4^unspecified (Freeman et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^unspecified has neuroanatomy defective \| larval stage phenotype, suppressible by kay^Fbz.Scer\UAS, Scer\GAL4^unspecified (Freeman et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^unspecified has neurophysiology defective \| larval stage phenotype, suppressible by CrebB^{Scer\UAS.b.cYa}, Scer\GAL4^unspecified (Freeman et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^unspecified has neurophysiology defective \| larval stage phenotype, suppressible by kay^Fbz.Scer\UAS, Scer\GAL4^unspecified (Freeman et al., 2010)
NOT suppressed by
Statement Reference Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^ey.3.5.Exel has visible phenotype, non-suppressible by CrebB^{Scer\UAS.b.cYa}, Scer\GAL4^ey.3.5.Exel (Freeman et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^ey.3.5.Exel has visible phenotype, non-suppressible by kay^Fbz.Scer\UAS, Scer\GAL4^ey.3.5.Exel (Freeman et al., 2010)
Suppressor of
Statement Reference Scer\GAL4^c747/Ras85D^{V12.S35.Scer\UAS} is a suppressor of learning defective phenotype of drk^ΔP24 (Moressis et al., 2009)
NOT Suppressor of
Statement Reference Scer\GAL4^c747, Scer\GAL80^ts.αTub84B, Ras85D^{V12.S35.Scer\UAS} is a non-suppressor of learning defective phenotype of drk^ΔP24 (Moressis et al., 2009)
Other
Statement Reference Rabex-5^VDRC.cUa, Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU has lethal phenotype (Yan et al., 2010) Rala^{S25N.cMa.Scer\UAS}, Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^sca.PU has lethal \| pupal stage phenotype (Mirey et al., 2003) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^tub.PU, Utx¹ has size defective \| somatic clone phenotype (Herz et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU (Yan et al., 2010)
Suppressed by
Statement Reference Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible \| partially by Rabex-5^{DPYT.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PU (Yan et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU has eye phenotype, suppressible \| partially by Rabex-5^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PU (Yan et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^unspecified has embryonic/larval neuromuscular junction \| larval stage phenotype, suppressible by CrebB^{Scer\UAS.b.cYa}, Scer\GAL4^unspecified (Freeman et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^unspecified has embryonic/larval neuromuscular junction \| larval stage phenotype, suppressible by kay^Fbz.Scer\UAS, Scer\GAL4^unspecified (Freeman et al., 2010)
NOT suppressed by
Statement Reference Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^ey.3.5.Exel has eye phenotype, non-suppressible by CrebB^{Scer\UAS.b.cYa}, Scer\GAL4^ey.3.5.Exel (Freeman et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^ey.3.5.Exel has eye phenotype, non-suppressible by kay^Fbz.Scer\UAS, Scer\GAL4^ey.3.5.Exel (Freeman et al., 2010)
Suppressor of
Statement Reference Scer\GAL4^hs.2sev/Ras85D^{V12.S35.Scer\UAS} is a suppressor of eye phenotype of W^GMR.PG/W^GMR.PG (Bergmann et al., 1998) Scer\GAL4^hs.2sev/Ras85D^{V12.S35.Scer\UAS} is a suppressor of ommatidium phenotype of W^GMR.PG/W^GMR.PG (Bergmann et al., 1998)
Other
Statement Reference Rabex-5^VDRC.cUa, Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU has antenna phenotype (Yan et al., 2010) Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^tub.PU, Utx¹ has eye \| somatic clone phenotype (Herz et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of either kay[Fbz.Scer\UAS] or CrebB-17A[Scer\UAS.b.cYa] suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in larvae expressing Ras85D[V12.S35.Scer\UAS] in the motor neurons under the control of Scer\GAL4[unspecified]. Co-expression of either kay[Fbz.Scer\UAS] or CrebB-17A[Scer\UAS.b.cYa] does not suppress the eye tumour phenotype caused by expression of Ras85D[V12.S35.Scer\UAS] under the control of Scer\GAL4[ey.3.5.Exel]. (Freeman et al., 2010) MARCM clones of Ras85D[V12.S35.Scer\UAS] in Utx[1] mosaic eyes result in mild overgrowth of the adult eye. (Herz et al., 2010) Co-expression of Rabex-5[VDRC.cUa] significantly enhances the number of eyes with black tissue and significantly increases lethality resulting from the expression of Ras85D[V12.S35.Scer\UAS] driven by Scer\GAL4[GMR.PU]. The overgrowth, extra antennae, and ectopic eye phenotypes of Ras85D[V12.S35.Scer\UAS] overexpression are also enhanced by Rabex-5[VDRC.cUa]-expression. Co-expression of Rabex-5[Scer\UAS.T:Hsap\MYC] driven by Scer\GAL4[GMR.PU] reduces the roughness, eye size, and black tissue phenotypes of Ras85D[V12.S35.Scer\UAS]-expressing eyes. The change in roughness is subtle. Co-expression of Rabex-5[DPYT.Scer\UAS.T:Hsap\MYC] driven by Scer\GAL4[GMR.PU] reduces the roughness, eye size, and black tissue phenotypes of Ras85D[V12.S35.Scer\UAS]-expressing eyes. The change in roughness is subtle. (Yan et al., 2010) Expression of Ras85D[V12.S35.Scer\UAS] alone, or combined with the Scer\GAL4[c747] driver but kept silent by Scer\GAL80[ts.αTub84B] does not suppress the learning deficit of drk[ΔP24]/+ animals. In contrast, expression of Ras85D[V12.S35.Scer\UAS] by inactivation of the Scer\GAL80[ts.αTub84B] suppressor restores normal learning to drk[ΔP24] heterozygotes, while it does not appear to affect learning in control animals. (Moressis et al., 2009) The enhancement effect of Ras85D^{V12.G37.Scer\UAS} on cnk^{hs.sev.T:Zzzz\FLAG} contrasts sharply with the lack of enhancement of cnk^{hs.sev.T:Zzzz\FLAG} by Ras85D^{V12.S35.Scer\UAS} or Ras85D^{V12.C40.Scer\UAS}. (Therrien et al., 1999) When expression is driven by Scer\GAL4^hs.2sev, acts as a strong suppressor of the W^GMR.PG eye phenotype. (Bergmann et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in a plasmid and transfected into S2 cells. (Douziech et al., 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Ras1V12S35 (Koh et al., 2002) Ras85D^{V12.S35.Scer\UAS} Ras85D^V12.S35.UAS Ras85D^V12S35 (Douziech et al., 2003, Gaengel and Mlodzik, 2003)
Name Synonym
Secondary FlyBase IDs

References ( 17 )
Research paper	Freeman et al., 2010, Brain Res. 1326: 15--29 A new genetic model of activity-induced Ras signaling dependent pre-synaptic plasticity in Drosophila. [FBrf0210453] Herz et al., 2010, Mol. Cell. Biol. 30(10): 2485--2497 The H3K27me3 demethylase dUTX is a suppressor of notch- and Rb-dependent tumors in Drosophila. [FBrf0210661] Yan et al., 2010, Curr. Biol. 20(15): 1378--1382 Rabex-5 Ubiquitin Ligase Activity Restricts Ras Signaling to Establish Pathway Homeostasis in Drosophila. [FBrf0211477] Jiang and Edgar, 2009, Development 136(3): 483--493 EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors. [FBrf0206542] Moressis et al., 2009, J. Neurosci. 29(8): 2611--2625 A dual role for the adaptor protein DRK in Drosophila olfactory learning and memory. [FBrf0207513] Yan et al., 2009, J. Cell Sci. 122(9): 1461--1470 Impairment of ubiquitylation by mutation in Drosophila E1 promotes both cell-autonomous and non-cell-autonomous Ras-ERK activation in vivo. [FBrf0207806] Asha et al., 2003, Genetics 163(1): 203--215 Analysis of ras-induced overproliferation in Drosophila hemocytes. [FBrf0155813] Botella et al., 2003, Mol. Biol. Cell 14(1): 241--250 Deregulation of the Egfr/Ras signaling pathway induces age-related brain degeneration in the Drosophila mutant vap. [FBrf0155942] Douziech et al., 2003, EMBO J. 22(19): 5068--5078 Bimodal regulation of RAF by CNK in Drosophila. [FBrf0167547] Gaengel and Mlodzik, 2003, Development 130(22): 5413--5423 Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. [FBrf0167499] Mirey et al., 2003, Mol. Cell. Biol. 23(3): 1112--1124 A Ral Guanine Exchange Factor-Ral Pathway Is Conserved in Drosophila melanogaster and Sheds New Light on the Connectivity of the Ral, Ras, and Rap Pathways. [FBrf0155960] Yang and Baker, 2003, Dev. Cell 4(3): 359--369 Cell cycle withdrawal, progression, and cell survival regulation by EGFR and its effectors in the differentiating Drosophila eye. [FBrf0158858] Koh et al., 2002, J. Neurosci. 22(7): 2496--2504 The Ras1-mitogen-activated protein kinase signal transduction pathway regulates synaptic plasticity through fasciclin II-mediated cell adhesion. [FBrf0151505] Prober and Edgar, 2002, Genes Dev. 16(17): 2286--2299 Interactions between Ras1, dMyc, and dPI3K signaling in the developing Drosophila wing. [FBrf0151998] Therrien et al., 1999, Proc. Natl. Acad. Sci. U.S.A. 96(23): 13259--13263 Functional analysis of CNK in RAS signaling. [FBrf0112056] Bergmann et al., 1998, Cell 95(3): 331--341 The Drosophila gene hid is a direct molecular target of Ras-dependent survival signaling. [FBrf0105197] Karim and Rubin, 1998, Development 125(1): 1--9 Ectopic expression of activated Ras1 induces hyperplastic growth and increased cell death in Drosophila imaginal tissues. [FBrf0100097]

Allele Dmel\Ras85DV12.S35.Scer\UAS

Allele Dmel\Ras85D^{V12.S35.Scer\UAS}