Construct: Amino acid replacement: T35S.
A mutation has been introduced into the effector loop (amino acid replacement T35S).
Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4esg-NP5130 in intestinal stem cell (ISC) somatic flip-out clones (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut results in massive clone overgrowth and highly increased ISC division.
Fly hearts expressing Scer\GAL4tin.CΔ4>Ras85DV12.S35.Scer\UAS have reduced end-diastolic dimensions compared with controls. Hearts isolated from the transgenic flies show abnormal cardiac morphology compared with controls. Histological analysis of cardiac chambers from adult transgenic flies show heart wall thickness that is similar to that of controls. The number of cardiomyocytes in the transgenic hearts is also similar to that of wild-type.
Expression of Ras85DV12.S35.Scer\UAS in the midgut, under the control of Scer\GAL4esg-NP7397 results in the generation of many new midgut cells, including enterocyte-like cells.
Expression of Ras85DV12.S35.Scer\UAS in the motor neurons under the control of Scer\GAL4unspecified results in increased synaptic growth and increased transmitter release at the neuromuscular junction.
Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel results in tumours in the eye.
Expression of Ras85DV12.S35.Scer\UAS in differentiating eye cells driven by Scer\GAL4GMR.PU causes rough eyes, eye overgrowth and other abnormalities.
Ectopic expression of Ras85DV12.S35.Scer\UAS driven by Scer\GAL4esg-NP7397 in adult midgut progenitor cells results in their overproliferation compared with control third instar larval midguts.
Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4ey.PH early in the entire eye results in dramatic overgrowth and an increase in organ size.
Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4Cg.PA results in an increase in hemocyte numbers in larvae compared to controls.
Ras85DV12.S35.Scer\UAS; Scer\GAL4elav-C155 is lethal.
Flies expressing Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4sca.PU show the development of extra macrochaetae in the vicinity of the normal macrochaetae on the notum and scutellum.
Caspase activation in cells posterior to the furrow in eye discs is completely suppressed by Ras85DV12.S35.Scer\UAS; Scer\GAL4GMR.PF. These discs also exhibit extensive ectopic photoreceptor differentiation posterior to the furrow.
Expression of Ras85DV12.S35.Scer\UAS, under the control of Scer\GAL4C380, induces a striking increase in the number of type I boutons at muscles 6 and 7 compared with controls expressing Ras85DScer\UAS.cKa and wild-type larvae. This increase is indistinguishable from the increase in bouton number observed in flies where Ras85DV12.Scer\UAS expression is driven by Scer\GAL4C380.
Expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4Act5C.PP in wing disc cells results in an increase in cell size and an increase in clone area compared to controls. The proportion of cells in G1 is decreased compared to controls. Clones expressing Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4Act5C.PP are significantly more round than control clones.
When expression is driven by Scer\GAL4sev.EP a rough eye phenotype results.
Scer\GAL4dpp.blk1-mediated expression causes hyperplastic growth in third instar wing discs.
Ras85DV12.S35.UAS, Scer\GAL4GMR.PU has visible phenotype, enhanceable by Rabex-5VDRC.cUa, Scer\GAL4GMR.PU
Ras85DV12.S35.UAS, Scer\GAL4sev.EP has visible phenotype, non-enhanceable by cnkhs.sev.Tag:FLAG
Ras85DV12.S35.UAS, Scer\GAL4unspecified has abnormal neuroanatomy | larval stage phenotype, suppressible by CrebBUAS.b.cYa, Scer\GAL4unspecified
Ras85DV12.S35.UAS, Scer\GAL4unspecified has abnormal neurophysiology | larval stage phenotype, suppressible by CrebBUAS.b.cYa, Scer\GAL4unspecified
Ras85DV12.S35.UAS, Scer\GAL4unspecified has abnormal neuroanatomy | larval stage phenotype, suppressible by kayFbz.UAS, Scer\GAL4unspecified
Ras85DV12.S35.UAS, Scer\GAL4unspecified has abnormal neurophysiology | larval stage phenotype, suppressible by kayFbz.UAS, Scer\GAL4unspecified
Ras85DV12.S35.UAS, Scer\GAL4GMR.PU has visible phenotype, suppressible | partially by Rabex-5UAS.Tag:MYC, Scer\GAL4GMR.PU
Ras85DV12.S35.UAS, Scer\GAL4GMR.PU has visible phenotype, suppressible | partially by Rabex-5DPYT.UAS.Tag:MYC, Scer\GAL4GMR.PU
Ras85DV12.S35.UAS, Scer\GAL4ey.3.5.Exel has visible phenotype, non-suppressible by kayFbz.UAS, Scer\GAL4ey.3.5.Exel
Ras85DV12.S35.UAS, Scer\GAL4ey.3.5.Exel has visible phenotype, non-suppressible by CrebBUAS.b.cYa, Scer\GAL4ey.3.5.Exel
Scer\GAL4c747/Ras85DV12.S35.UAS is a suppressor of abnormal learning phenotype of drkΔP24
Scer\GAL4c747, Scer\GAL80ts.αTub84B, Ras85DV12.S35.UAS is a non-suppressor of abnormal learning phenotype of drkΔP24
Ras85DV12.S35.UAS, Scer\GAL4Tub.PU, Utx1 has abnormal size | somatic clone phenotype
Rabex-5VDRC.cUa, Ras85DV12.S35.UAS, Scer\GAL4GMR.PU has lethal phenotype
RalaS25N.cMa.UAS, Ras85DV12.S35.UAS, Scer\GAL4sca.PU has lethal | pupal stage phenotype
Ras85DV12.S35.UAS, Scer\GAL4GMR.PU has eye phenotype, enhanceable by Rabex-5VDRC.cUa, Scer\GAL4GMR.PU
Ras85DV12.S35.UAS, Scer\GAL4NP5130 has adult midgut | somatic clone phenotype, suppressible | partially by cicUAS.Tag:HA, Scer\GAL4NP5130
Ras85DV12.S35.UAS, Scer\GAL4NP5130 has intestinal stem cell | somatic clone | adult stage phenotype, suppressible | partially by cicUAS.Tag:HA, Scer\GAL4NP5130
Ras85DV12.S35.UAS, Scer\GAL4NP5130 has adult midgut | somatic clone phenotype, suppressible | partially by cicΔC2.UAS.Tag:HA, Scer\GAL4NP5130
Ras85DV12.S35.UAS, Scer\GAL4NP5130 has intestinal stem cell | somatic clone | adult stage phenotype, suppressible | partially by cicΔC2.UAS.Tag:HA, Scer\GAL4NP5130
Ras85DV12.S35.UAS, Scer\GAL4unspecified has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by kayFbz.UAS, Scer\GAL4unspecified
Ras85DV12.S35.UAS, Scer\GAL4unspecified has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by CrebBUAS.b.cYa, Scer\GAL4unspecified
Ras85DV12.S35.UAS, Scer\GAL4GMR.PU has eye phenotype, suppressible | partially by Rabex-5UAS.Tag:MYC, Scer\GAL4GMR.PU
Ras85DV12.S35.UAS, Scer\GAL4GMR.PU has eye phenotype, suppressible | partially by Rabex-5DPYT.UAS.Tag:MYC, Scer\GAL4GMR.PU
Ras85DV12.S35.UAS, Scer\GAL4ey.3.5.Exel has eye phenotype, non-suppressible by kayFbz.UAS, Scer\GAL4ey.3.5.Exel
Ras85DV12.S35.UAS, Scer\GAL4ey.3.5.Exel has eye phenotype, non-suppressible by CrebBUAS.b.cYa, Scer\GAL4ey.3.5.Exel
Scer\GAL4hs.2sev/Ras85DV12.S35.UAS is a suppressor of eye phenotype of hidGMR.PG/WGMR.PG
Scer\GAL4hs.2sev/Ras85DV12.S35.UAS is a suppressor of ommatidium phenotype of hidGMR.PG/WGMR.PG
Ras85DV12.S35.UAS, Scer\GAL4Tub.PU, Utx1 has eye | somatic clone phenotype
Rabex-5VDRC.cUa, Ras85DV12.S35.UAS, Scer\GAL4GMR.PU has antenna phenotype
The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing btl::EgfrScer\UAS.T:λ\cI-DD under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut is partially suppressed by co-expression of either cicScer\UAS.T:Ivir\HA1 or cicΔC2.Scer\UAS.T:Ivir\HA1.
The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing cicKK100838 under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut cannot be suppressed by co-expression of Ras85DdsRNA.Scer\UAS.cUa.
Co-expression of either kayFbz.Scer\UAS or CrebB-17AScer\UAS.b.cYa suppresses the increased synaptic growth and transmitter release observed at the neuromuscular junction in larvae expressing Ras85DV12.S35.Scer\UAS in the motor neurons under the control of Scer\GAL4unspecified.
Co-expression of either kayFbz.Scer\UAS or CrebB-17AScer\UAS.b.cYa does not suppress the eye tumour phenotype caused by expression of Ras85DV12.S35.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel.
MARCM clones of Ras85DV12.S35.Scer\UAS in Utx1 mosaic eyes result in mild overgrowth of the adult eye.
Co-expression of Rabex-5VDRC.cUa significantly enhances the number of eyes with black tissue and significantly increases lethality resulting from the expression of Ras85DV12.S35.Scer\UAS driven by Scer\GAL4GMR.PU. The overgrowth, extra antennae, and ectopic eye phenotypes of Ras85DV12.S35.Scer\UAS overexpression are also enhanced by Rabex-5VDRC.cUa-expression.
Co-expression of Rabex-5Scer\UAS.T:Hsap\MYC driven by Scer\GAL4GMR.PU reduces the roughness, eye size, and black tissue phenotypes of Ras85DV12.S35.Scer\UAS-expressing eyes. The change in roughness is subtle.
Co-expression of Rabex-5DPYT.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4GMR.PU reduces the roughness, eye size, and black tissue phenotypes of Ras85DV12.S35.Scer\UAS-expressing eyes. The change in roughness is subtle.
Expression of Ras85DV12.S35.Scer\UAS alone, or combined with the Scer\GAL4c747 driver but kept silent by Scer\GAL80ts.αTub84B does not suppress the learning deficit of drkΔP24/+ animals. In contrast, expression of Ras85DV12.S35.Scer\UAS by inactivation of the Scer\GAL80ts.αTub84B suppressor restores normal learning to drkΔP24 heterozygotes, while it does not appear to affect learning in control animals.
The enhancement effect of Ras85DV12.G37.Scer\UAS on cnkhs.sev.T:Zzzz\FLAG contrasts sharply with the lack of enhancement of cnkhs.sev.T:Zzzz\FLAG by Ras85DV12.S35.Scer\UAS or Ras85DV12.C40.Scer\UAS.
When expression is driven by Scer\GAL4hs.2sev, acts as a strong suppressor of the WGMR.PG eye phenotype.
Carried in a plasmid and transfected into S2 cells.