Allele Dmel\Ras85D^Scer\UAS.cKa

General Information
Symbol	Dmel\Ras85D^Scer\UAS.cKa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Karim	FlyBase ID	FBal0085939
Feature type	allele	Associated gene	Dmel\Ras85D

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Karim and Rubin, 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Genomic fragment carrying wild type Ras85D sequences is cloned into the pUAST vector. (Karim and Rubin, 1998)
Carried in construct	P{UAS-Ras85D.K} (Botella et al., 2003, Asha et al., 2003, Caldwell et al., 2005, Caldwell et al., 2005, Karim and Rubin, 1998, Lee et al., 2002, Prober and Edgar, 2000, Bui et al., 2000, Bergmann et al., 1998, Kim et al., 2012, Wu et al., 2009, Salzer et al., 2010, Sung et al., 2010, Shen et al., 2009, Dupuy et al., 2005, Morris et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| heat sensitive, with Scer\GAL4^Bx-MS1096 (Feldmann et al., 1999) neuroanatomy defective, with Scer\GAL4^futsch-C380 (Koh et al., 2002) neuroanatomy defective, with Scer\GAL4^sca.PC (Koh et al., 2002) viable, with Scer\GAL4^Cg.PA (Asha et al., 2003) visible, with Scer\GAL4^Bx-MS1096 (Feldmann et al., 1999) visible, with Scer\GAL4^en.PU (Kim et al., 2012) visible, with Scer\GAL4^GMR.PF (Morris et al., 2012)
Phenotype Manifest In
	bouton \| supernumerary, with Scer\GAL4^futsch-C380 (Koh et al., 2002) bouton \| supernumerary, with Scer\GAL4^sca.PC (Koh et al., 2002) embryonic/larval hemocyte \| supernumerary, with Scer\GAL4^Cg.PA (Asha et al., 2003) ommatidium, with Scer\GAL4^GMR.PF (Morris et al., 2012) wing, with Scer\GAL4^Bx-MS1096 (Feldmann et al., 1999) wing disc posterior compartment, with Scer\GAL4^en.PU (Kim et al., 2012) wing vein \| ectopic, with Scer\GAL4^en.PU (Kim et al., 2012)
Detailed Description
	Statement Reference Extra veins arise in the posterior compartment of the wing when Ras85D[Scer\UAS.cKa] is expressed under the control of Scer\GAL4[en.PU]. (Kim et al., 2012) Expression of Ras85D[Scer\UAS.cKa] with the Scer\GAL4[GMR.PF] driver produces a rough eye phenotype that results from fusion and irregular alignment of individual ommatidia. (Morris et al., 2012) Expression of Ras85D[Scer\UAS.cKa] in the larvae under the control of Scer\GAL4[Act5C.Switch.PR] does not result in lethality. Both male and female life-span not reduced significantly when Ras85D[V12.Scer\UAS] is expressed in adult flies. (Shen et al., 2009) Flies that express Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^amn-c651, exhibit normal body size, with normal-sized prothoracic gland cells. (Caldwell et al., 2005) Larvae expressing Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^Cg.PA show a mild increase in the number of hemocytes compared to wild type. (Asha et al., 2003) Overexpression of Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^C380, at both presynaptic and postsynaptic cells or at presynaptic cells (using Scer\GAL4^sca.PC) alone causes an increase in the number of boutons. (Koh et al., 2002) Flies expressing Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^Bx-MS1096 do not eclose at 25^oC, at 18^oC the flies eclose but have abnormal wings. (Feldmann et al., 1999) Scer\GAL4^dpp.blk1-mediated expression causes very little cell death. (Karim and Rubin, 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Ras85D^Scer\UAS.cKa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Ras85D^Scer\UAS.cKa (Morris et al., 2012, Morris et al., 2012)
Enhancer of
Statement Reference Ras85D^Scer\UAS.cKa, Scer\GAL4^en.PU is an enhancer of visible phenotype of Scer\GAL4^en.PU, pum^VDRC.cUa (Kim et al., 2012)
Suppressor of
Statement Reference Ras85D^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Lee et al., 2002) Ras85D^Scer\UAS.cKa is a suppressor of visible phenotype of Ras85D^Scer\UAS.cKa, Scer\GAL4^GMR.PF (Morris et al., 2012, Morris et al., 2012)
NOT Suppressor of
Statement Reference Ras85D^Scer\UAS.cKa, Scer\GAL4^GMR.PF is a non-suppressor of lethal phenotype of R^{S17A.Scer\UAS}, Scer\GAL4^GMR.PF (Dupuy et al., 2005)
Phenotype Manifest In
Suppressed by
Statement Reference Ras85D^Scer\UAS.cKa, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Ras85D^Scer\UAS.cKa (Morris et al., 2012, Morris et al., 2012)
Enhancer of
Statement Reference Ras85D^Scer\UAS.cKa, Scer\GAL4^en.PU is an enhancer of wing vein \| ectopic phenotype of Scer\GAL4^en.PU, pum^VDRC.cUa (Kim et al., 2012) Ras85D^Scer\UAS.cKa/Scer\GAL4^Appl.G1a is an enhancer of optic lobe \| adult stage phenotype of vap² (Botella et al., 2003)
Suppressor of
Statement Reference Ras85D^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Lee et al., 2002) Ras85D^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye photoreceptor cell \| ectopic phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Lee et al., 2002) Ras85D^Scer\UAS.cKa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Lee et al., 2002) Ras85D^Scer\UAS.cKa is a suppressor of ommatidium phenotype of Ras85D^Scer\UAS.cKa, Scer\GAL4^GMR.PF (Morris et al., 2012, Morris et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Overexpression of Ras85D[Scer\UAS.cKa] greatly enhances the extra wing vein phenotype resulting from the expression of pum[VDRC.cUa] via Scer\GAL4[en.PU]. (Kim et al., 2012) The rough eye phenotype resulting from the expression of Ras85D[Scer\UAS.cKa] via Scer\GAL4[GMR.PF] is suppressed by homozygous CRMP[supK1]. (Morris et al., 2012) The lethality caused by expression of R[S17A.Scer\UAS] under the control of Scer\GAL4[GMR.PF] is not rescued by co-expression of Ras85D[Scer\UAS.cKa]. (Dupuy et al., 2005) Flies which are co-expressing Ras85D^Scer\UAS.cKa and Gef26^EP388 under the control of Scer\GAL4^GMR.PF have a rather normal eye, with a normal ommatidial array and photoreceptor structure. (Lee et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Ras85D^Scer\UAS.cKa, Scer\GAL80^ts.αTub84B, Scer\GAL4^αTub84B.PL rescues Ras85D^ΔC40B (Sung et al., 2010) Scer\GAL4^Act.PU/Ras85D^Scer\UAS.cKa rescues Ras85D^KP (Wu et al., 2009) Scer\GAL4^en-e16E/Ras85D^Scer\UAS.cKa rescues Ras85D^ΔC40B (Prober and Edgar, 2000)
Comments	Expression of Ras85D[Scer\UAS.cKa] under the control of Scer\GAL4[αTub84B.PL] in combination with Scer\GAL80[ts.αTub84B] in eye disc clones rescues the ommatidial development defects of Ras85D[ΔC40B] homozygous cells. (Sung et al., 2010) Expression of Ras85D[Scer\UAS.cKa] under the control of Scer\GAL4[Act] in Ras85D[KP] mutant animals fully rescues the mutant eye phenotype. (Wu et al., 2009) The size and survival of homozygous Ras85D^ΔC40B clones in the imaginal disc is completely rescued by expression of Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E. (Prober and Edgar, 2000)
Stocks ( 2 )
Bloomington	5788 w^*; P{UAS-Ras85D.K}5-1
Kyoto	108424 w^*; P{UAS-Ras85D.K}5-1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Ras1WT (Koh et al., 2002) Ras85D^Scer\UAS.cKa Ras85D^UAS.cKa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Karim (Karim and Rubin, 1998)
Secondary FlyBase IDs

References ( 18 )
Research paper	Kim et al., 2012, PLoS ONE 7(4): e34016 Negative Regulation of EGFR/MAPK Pathway by Pumilio in Drosophila melanogaster. [FBrf0218126] Morris et al., 2012, Genetics 191(4): 1227--1238 Divergent Functions Through Alternative Splicing: The Drosophila CRMP Gene in Pyrimidine Metabolism, Brain, and Behavior. [FBrf0219149] Salzer et al., 2010, Genetics 184(1): 185--197 The retinal determination gene eyes absent is regulated by the EGF receptor pathway throughout development in Drosophila. [FBrf0209690] Sung et al., 2010, Mol. Cell. Biol. 30(24): 5649--5657 Cytosolic ras supports eye development in Drosophila. [FBrf0212407] Shen et al., 2009, Aging 1(2): 191--211 A screen of apoptosis and senescence regulatory genes for life span effects when over-expressed in Drosophila. [FBrf0209964] Wu et al., 2009, BMC Dev. Biol. 9: 53 Ras promotes cell survival by antagonizing both JNK and Hid signals in the Drosophila eye. [FBrf0209242] Caldwell et al., 2005, Curr. Biol. 15(20): 1785--1795 Ras activity in the Drosophila prothoracic gland regulates body size and developmental rate via ecdysone release. [FBrf0190001] Dupuy et al., 2005, Oncogene 24(28): 4509--4520 Novel Rap1 dominant-negative mutants interfere selectively with C3G and Epac. [FBrf0188198] Asha et al., 2003, Genetics 163(1): 203--215 Analysis of ras-induced overproliferation in Drosophila hemocytes. [FBrf0155813] Botella et al., 2003, Mol. Biol. Cell 14(1): 241--250 Deregulation of the Egfr/Ras signaling pathway induces age-related brain degeneration in the Drosophila mutant vap. [FBrf0155942] Koh et al., 2002, J. Neurosci. 22(7): 2496--2504 The Ras1-mitogen-activated protein kinase signal transduction pathway regulates synaptic plasticity through fasciclin II-mediated cell adhesion. [FBrf0151505] Lee et al., 2002, Mol. Cell. Biol. 22(21): 7658--7666 Drosophila PDZ-GEF, a guanine nucleotide exchange factor for Rap1 GTPase, reveals a novel upstream regulatory mechanism in the mitogen-activated protein kinase signaling pathway. [FBrf0152217] Bui et al., 2000, Dev. Biol. 221(2): 355--364 Functional analysis of an eye enhancer of the Drosophila eyes absent gene: differential regulation by eye specification genes. [FBrf0127029] Prober and Edgar, 2000, Cell 100(4): 435--446 Ras1 promotes cellular growth in the Drosophila wing. [FBrf0125350] Feldmann et al., 1999, Mol. Cell. Biol. 19(3): 1928--1937 Control of growth and differentiation by Drosophila RasGAP, a homolog of p120 ras-GTPase-activating protein. [FBrf0106478] Bergmann et al., 1998, Cell 95(3): 331--341 The Drosophila gene hid is a direct molecular target of Ras-dependent survival signaling. [FBrf0105197] Karim and Rubin, 1998, Development 125(1): 1--9 Ectopic expression of activated Ras1 induces hyperplastic growth and increased cell death in Drosophila imaginal tissues. [FBrf0100097]
Erratum	Caldwell et al., 2005, Curr. Biol. 15(23): 2175 Erratum to "Ras activity in the Drosophila prothoracic gland regulates body size and developmental rate via ecdysone release" (vol 15, pg 1785, 2005). [FBrf0190000]

Allele Dmel\Ras85DScer\UAS.cKa

Allele Dmel\Ras85D^Scer\UAS.cKa