FlyBase Allele Report: Dmel\Ras85D[UAS.cKa]

General Information

Symbol

Dmel\Ras85D^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Karim

FlyBase ID

FBal0085939

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{UAS-Ras85D.K}

Also Known As

UAS-ras, UAS-Ras^wt, UAS-Ras1

Key Links

Transgenic product class

genomic_DNA

(Karim and Rubin, 1998)

wild_type

(Karim and Rubin, 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Karim and Rubin, 1998)

wild_type

(Karim and Rubin, 1998)

Mutagen

in vitro construct

(Karim and Rubin, 1998)

Progenitor genotype

Carried in construct

P{UAS-Ras85D.K}

Cytology

Description

UASt regulatory sequences drive expression of wild-type Ras85D (genomic fragment).

(Karim and Rubin, 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ras85D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^futsch-C380

(Koh et al., 2002)

abnormal neuroanatomy, with Scer\GAL4^sca.PC

(Koh et al., 2002)

lethal | heat sensitive, with Scer\GAL4^Bx-MS1096

(Feldmann et al., 1999)

viable, with Scer\GAL4^Cg.PA

(Asha et al., 2003)

visible, with Scer\GAL4^Bx-MS1096

(Feldmann et al., 1999)

visible, with Scer\GAL4^en.PU

(Kim et al., 2012)

visible, with Scer\GAL4^GMR.PF

(Morris et al., 2012)

Phenotype Manifest In

bouton | increased number, with Scer\GAL4^futsch-C380

(Koh et al., 2002)

bouton | increased number, with Scer\GAL4^sca.PC

(Koh et al., 2002)

embryonic/larval hemocyte | increased number, with Scer\GAL4^Cg.PA

(Asha et al., 2003)

ommatidium, with Scer\GAL4^GMR.PF

(Morris et al., 2012)

wing, with Scer\GAL4^Bx-MS1096

(Feldmann et al., 1999)

wing disc posterior compartment, with Scer\GAL4^en.PU

(Kim et al., 2012)

wing vein | ectopic, with Scer\GAL4^en.PU

(Kim et al., 2012)

Detailed Description

Statement

Reference

Expression of Ras85D^Scer\UAS.cKa driven by Scer\GAL4^dpp.PU does not lead to tissue overgrowth in eye-antennal imaginal or wing discs (or in wing discs driven by Scer\GAL4^Bx-MS1096).

(Snee et al., 2016)

Extra veins arise in the posterior compartment of the wing when Ras85D^Scer\UAS.cKa is expressed under the control of Scer\GAL4^en.PU.

(Kim et al., 2012)

Expression of Ras85D^Scer\UAS.cKa with the Scer\GAL4^GMR.PF driver produces a rough eye phenotype that results from fusion and irregular alignment of individual ommatidia.

(Morris et al., 2012)

Expression of Ras85D^Scer\UAS.cKa in the larvae under the control of Scer\GAL4^{Act5C.Switch.PR} does not result in lethality. Both male and female life-span not reduced significantly when Ras85D^V12.Scer\UAS is expressed in adult flies.

(Shen et al., 2009)

Flies that express Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^amn-c651, exhibit normal body size, with normal-sized prothoracic gland cells.

(Caldwell et al., 2005)

Larvae expressing Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^Cg.PA show a mild increase in the number of hemocytes compared to wild type.

(Asha et al., 2003)

Overexpression of Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^C380, at both presynaptic and postsynaptic cells or at presynaptic cells (using Scer\GAL4^sca.PC) alone causes an increase in the number of boutons.

(Koh et al., 2002)

Flies expressing Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^Bx-MS1096 do not eclose at 25^oC, at 18^oC the flies eclose but have abnormal wings.

(Feldmann et al., 1999)

Scer\GAL4^dpp.blk1-mediated expression causes very little cell death.

(Karim and Rubin, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ras85D^UAS.cKa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Ras85D^UAS.cKa

(Morris et al., 2012)

Enhancer of

Statement

Reference

Ras85D^UAS.cKa, Scer\GAL4^en.PU is an enhancer of visible phenotype of Scer\GAL4^en.PU, pum^RNAi.UAS.cUa

(Kim et al., 2012)

NOT Enhancer of

Statement

Reference

Ras85D^UAS.cKa, Scer\GAL4^GMR.PU is a non-enhancer of visible | adult stage phenotype of Hsap\KCNC3^R423H.UAS, Scer\GAL4^GMR.PU

(Khare et al., 2017)

Suppressor of

Statement

Reference

Ras85D^UAS.cKa is a suppressor of visible phenotype of Ras85D^UAS.cKa, Scer\GAL4^GMR.PF

(Morris et al., 2012)

Ras85D^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF

(Lee et al., 2002)

NOT Suppressor of

Statement

Reference

Ras85D^UAS.cKa, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\KCNC3^R423H.UAS, Scer\GAL4^GMR.PU

(Khare et al., 2017)

Ras85D^UAS.cKa, Scer\GAL4^GMR.PF is a non-suppressor of lethal phenotype of Rap1^S17A.UAS, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ras85D^UAS.cKa, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Ras85D^UAS.cKa

(Morris et al., 2012)

Enhancer of

Statement

Reference

Ras85D^UAS.cKa, Scer\GAL4^en.PU is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^en.PU, pum^RNAi.UAS.cUa

(Kim et al., 2012)

Ras85D^UAS.cKa/Scer\GAL4^Appl.G1a is an enhancer of adult optic lobe | adult stage phenotype of vap²

(Botella et al., 2003)

NOT Enhancer of

Statement

Reference

Ras85D^UAS.cKa, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Hsap\KCNC3^R423H.UAS, Scer\GAL4^GMR.PU

(Khare et al., 2017)

Suppressor of

Statement

Reference

Ras85D^UAS.cKa is a suppressor of ommatidium phenotype of Ras85D^UAS.cKa, Scer\GAL4^GMR.PF

(Morris et al., 2012)

Ras85D^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF

(Lee et al., 2002)

Ras85D^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of eye photoreceptor cell | ectopic phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF

(Lee et al., 2002)

Ras85D^UAS.cKa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF

(Lee et al., 2002)

NOT Suppressor of

Statement

Reference

Ras85D^UAS.cKa, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\KCNC3^R423H.UAS, Scer\GAL4^GMR.PU

(Khare et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Dis3^KK101473 and Ras85D^Scer\UAS.cKa driven by Scer\GAL4^dpp.PU does not lead to tissue overgrowth in eye-antennal imaginal or wing discs (or in wing discs driven by Scer\GAL4^Bx-MS1096).

(Snee et al., 2016)

Overexpression of Ras85D^Scer\UAS.cKa greatly enhances the extra wing vein phenotype resulting from the expression of pum^VDRC.cUa via Scer\GAL4^en.PU.

(Kim et al., 2012)

The rough eye phenotype resulting from the expression of Ras85D^Scer\UAS.cKa via Scer\GAL4^GMR.PF is suppressed by homozygous CRMP^supK1.

(Morris et al., 2012)

The lethality caused by expression of R^{S17A.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not rescued by co-expression of Ras85D^Scer\UAS.cKa.

(Dupuy et al., 2005)

Flies which are co-expressing Ras85D^Scer\UAS.cKa and Gef26^EP388 under the control of Scer\GAL4^GMR.PF have a rather normal eye, with a normal ommatidial array and photoreceptor structure.

(Lee et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Ras85D^UAS.cKa, Scer\GAL80^ts.αTub84B, Scer\GAL4^αTub84B.PL rescues Ras85D^ΔC40B

(Sung et al., 2010)

Scer\GAL4^Act.PU/Ras85D^UAS.cKa rescues Ras85D^KP

(Wu et al., 2009)

Scer\GAL4^en-e16E/Ras85D^UAS.cKa rescues Ras85D^ΔC40B

(Prober and Edgar, 2000)

Comments

Expression of Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^αTub84B.PL in combination with Scer\GAL80^ts.αTub84B in eye disc clones rescues the ommatidial development defects of Ras85D^ΔC40B homozygous cells.

(Sung et al., 2010)

Expression of Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^Act in Ras85D^KP mutant animals fully rescues the mutant eye phenotype.

(Wu et al., 2009)

The size and survival of homozygous Ras85D^ΔC40B clones in the imaginal disc is completely rescued by expression of Ras85D^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E.

(Prober and Edgar, 2000)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5788

w^*; P{UAS-Ras85D.K}5-1

Kyoto

108424

w^*; P{UAS-Ras85D.K}5-1

Notes on Origin