Open Close
General Information
Symbol
Dmel\Ras85DUAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Karim
FlyBase ID
FBal0085939
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ras, UAS-Raswt, UAS-Ras1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of wild-type Ras85D (genomic fragment).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Ras85DScer\UAS.cKa driven by Scer\GAL4dpp.PU does not lead to tissue overgrowth in eye-antennal imaginal or wing discs (or in wing discs driven by Scer\GAL4Bx-MS1096).

Extra veins arise in the posterior compartment of the wing when Ras85DScer\UAS.cKa is expressed under the control of Scer\GAL4en.PU.

Expression of Ras85DScer\UAS.cKa with the Scer\GAL4GMR.PF driver produces a rough eye phenotype that results from fusion and irregular alignment of individual ommatidia.

Expression of Ras85DScer\UAS.cKa in the larvae under the control of Scer\GAL4Act5C.Switch.PR does not result in lethality. Both male and female life-span not reduced significantly when Ras85DV12.Scer\UAS is expressed in adult flies.

Flies that express Ras85DScer\UAS.cKa under the control of Scer\GAL4amn-c651, exhibit normal body size, with normal-sized prothoracic gland cells.

Larvae expressing Ras85DScer\UAS.cKa under the control of Scer\GAL4Cg.PA show a mild increase in the number of hemocytes compared to wild type.

Overexpression of Ras85DScer\UAS.cKa under the control of Scer\GAL4C380, at both presynaptic and postsynaptic cells or at presynaptic cells (using Scer\GAL4sca.PC) alone causes an increase in the number of boutons.

Flies expressing Ras85DScer\UAS.cKa under the control of Scer\GAL4Bx-MS1096 do not eclose at 25oC, at 18oC the flies eclose but have abnormal wings.

Scer\GAL4dpp.blk1-mediated expression causes very little cell death.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Dis3KK101473 and Ras85DScer\UAS.cKa driven by Scer\GAL4dpp.PU does not lead to tissue overgrowth in eye-antennal imaginal or wing discs (or in wing discs driven by Scer\GAL4Bx-MS1096).

Overexpression of Ras85DScer\UAS.cKa greatly enhances the extra wing vein phenotype resulting from the expression of pumVDRC.cUa via Scer\GAL4en.PU.

The rough eye phenotype resulting from the expression of Ras85DScer\UAS.cKa via Scer\GAL4GMR.PF is suppressed by homozygous CRMPsupK1.

The lethality caused by expression of RS17A.Scer\UAS under the control of Scer\GAL4GMR.PF is not rescued by co-expression of Ras85DScer\UAS.cKa.

Flies which are co-expressing Ras85DScer\UAS.cKa and Gef26EP388 under the control of Scer\GAL4GMR.PF have a rather normal eye, with a normal ommatidial array and photoreceptor structure.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Ras85DScer\UAS.cKa under the control of Scer\GAL4αTub84B.PL in combination with Scer\GAL80ts.αTub84B in eye disc clones rescues the ommatidial development defects of Ras85DΔC40B homozygous cells.

Expression of Ras85DScer\UAS.cKa under the control of Scer\GAL4Act in Ras85DKP mutant animals fully rescues the mutant eye phenotype.

The size and survival of homozygous Ras85DΔC40B clones in the imaginal disc is completely rescued by expression of Ras85DScer\UAS.cKa under the control of Scer\GAL4en-e16E.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Ras85DScer\UAS.cKa
Ras85DUAS.cKa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Karim
Secondary FlyBase IDs
    References (23)