Allele Dmel\sty^Scer\UAS.cHa

General Information
Symbol	Dmel\sty^Scer\UAS.cHa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Hacohen	FlyBase ID	FBal0086309
Feature type	allele	Associated gene	Dmel\sty

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Hacohen et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A 2.2kb sty cDNA fragment containing the entire coding region is expressed under the control of Scer\UAS regulatory sequences. (Hacohen et al., 1998)
Carried in construct	P{UAS-sty.H} (Iwanami et al., 2005, Reich et al., 1999, Bai et al., 2001, Kumar and Moses, 2001, Kramer et al., 1999, Hacohen et al., 1998, Kumar and Moses, 2001, Jarvis et al., 2006, Dutta et al., 2005, Mao and Freeman, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^GMR.PF (Bai et al., 2001) visible, with Scer\GAL4^hs.2sev (Jarvis et al., 2006)
Phenotype Manifest In
	chordotonal organ, with Scer\GAL4^en-e16E (Kramer et al., 1999) dorsal appendage, with Scer\GAL4^T155 (Reich et al., 1999) egg, with Scer\GAL4^CY2 (Kramer et al., 1999) egg, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Reich et al., 1999) egg, with Scer\GAL4^T155 (Reich et al., 1999) embryo, with Scer\GAL4^CY2 (Kramer et al., 1999) eye, with Scer\GAL4^elav-C155 (Kramer et al., 1999) eye, with Scer\GAL4^GMR.PF (Bai et al., 2001) eye, with Scer\GAL4^hs.2sev (Kramer et al., 1999) eye, with Scer\GAL4^sev.EP (Mao and Freeman, 2009) eye photoreceptor cell, with Scer\GAL4^hs.2sev (Jarvis et al., 2006) interommatidial bristle, with Scer\GAL4^hs.2sev (Jarvis et al., 2006) muscle founder cell, with Scer\GAL4¹¹⁵¹ (Dutta et al., 2005) ommatidium, with Scer\GAL4^elav-C155 (Kramer et al., 1999) ommatidium, with Scer\GAL4^hs.2sev (Kramer et al., 1999, Jarvis et al., 2006) photoreceptor cell R1, with Scer\GAL4^elav-C155 (Kramer et al., 1999) photoreceptor cell R1, with Scer\GAL4^hs.2sev (Kramer et al., 1999) photoreceptor cell R3, with Scer\GAL4^elav-C155 (Kramer et al., 1999) photoreceptor cell R3, with Scer\GAL4^hs.2sev (Kramer et al., 1999) photoreceptor cell R4, with Scer\GAL4^elav-C155 (Kramer et al., 1999) photoreceptor cell R4, with Scer\GAL4^hs.2sev (Kramer et al., 1999) photoreceptor cell R6, with Scer\GAL4^elav-C155 (Kramer et al., 1999) photoreceptor cell R6, with Scer\GAL4^hs.2sev (Kramer et al., 1999) photoreceptor cell R7, with Scer\GAL4^elav-C155 (Kramer et al., 1999) photoreceptor cell R7, with Scer\GAL4^hs.2sev (Kramer et al., 1999) wing, with Scer\GAL4^BH1 (Kramer et al., 1999) wing, with Scer\GAL4^Bx-MS1096 (Reich et al., 1999) wing vein, with Scer\GAL4^BH1 (Kramer et al., 1999) wing vein L2, with Scer\GAL4^Bx-MS1096 (Reich et al., 1999) wing vein L3, with Scer\GAL4^Bx-MS1096 (Reich et al., 1999) wing vein L3, with Scer\GAL4^dpp.blk1 (Reich et al., 1999) wing vein L4, with Scer\GAL4^Bx-MS1096 (Reich et al., 1999)
Detailed Description
	Statement Reference Expression of sty[Scer\UAS.cHa] under the control of Scer\GAL4[sev.EP] results in a rough-eye phenotype. (Mao and Freeman, 2009) Expression of sty^Scer\UAS.cHa under the control of Scer\GAL4^hs.2sev causes misrotation and disorganization of ommatidia, missing photoreceptors in 5% of ommatidia, and external roughening of the eye. (Jarvis et al., 2006) Expression of sty^Scer\UAS.cHa under the control of Scer\GAL4¹¹⁵¹ leads to a decrease in the number of founder cells; there are ~7 dorsal founders and ~8 lateral founders per hemisegment in Scer\GAL4¹¹⁵¹>sty^Scer\UAS.cHa pupae, compared to 17-22 and 20 of these founder types in control pupae. However, there is no comparable change in the number of fusion-competent myoblasts. (Dutta et al., 2005) When expression is driven by Scer\GAL4^GMR.PF eyes are small and rough. (Bai et al., 2001) Expression of sty^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 has no effect on furrow initiation in the eye disc. (Kumar and Moses, 2001) When expression is driven by Scer\GAL4^hs.2sev or Scer\GAL4^elav-C155 the eyes of the resulting flies are small and disorganized. Majority of ommatidia lack one or more outer photoreceptor cells of the R3/R4/R1/R6 type. The R7 is missing in 11+-4% of Scer\GAL4^hs.2sev-driven ommatidia and 18+-6% of Scer\GAL4^elav-C155-driven ommatidia. R2/R5 and R8 are unaffected. When expression is driven by Scer\GAL4^en-e16E, the number of chordotonal organs in the embryo is reduced. When expression is driven by Scer\GAL4^BH1, wing veins fail to form, and the size of the wing blade is reduced. This phenotype is similar to that caused by loss of function Egfr or vn mutants. When expression is driven by Scer\GAL4^CY2, resulting eggs had a ventralized egg shell and the resulting embryos were also ventralized. This phenotype is similar to that caused by Egfr loss of function mutants. (Kramer et al., 1999) When expression is driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} the resulting phenotype resembles that caused by loss of function alleles in Egfr. When expression is driven by Scer\GAL4^T155 the egg is ventralized and the dorsal appendages are fused and reduced. When expression is driven by Scer\GAL4^dpp.blk1 the L3 vein of the adult wing is truncated. When expression is driven by Scer\GAL4^Bx-MS1096 the L2, L3 and L4 veins of the adult wing are truncated. The size of the wing is somewhat reduced. (Reich et al., 1999)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, sty^Scer\UAS.cHa has visible phenotype, enhanceable by ed^Scer\UAS.cBa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bai et al., 2001)
Suppressed by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, sty^Scer\UAS.cHa has visible phenotype, suppressible \| partially by ed^lF20 (Bai et al., 2001)
Suppressor of
Statement Reference Scer\GAL4^71B/sty^Scer\UAS.cHa is a suppressor of lethal phenotype of Scer\GAL4^71B, phl^{Scer\UAS.F179} (Reich et al., 1999)
Other
Statement Reference Scer\GAL4^71B, phl^{Scer\UAS.F179}, sty^Scer\UAS.cHa has visible phenotype (Reich et al., 1999, Reich et al., 1999)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, sty^Scer\UAS.cHa has eye phenotype, enhanceable by ed^Scer\UAS.cBa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Bai et al., 2001)
Suppressed by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, sty^Scer\UAS.cHa has eye phenotype, suppressible \| partially by ed^lF20 (Bai et al., 2001) Scer\GAL4^sev.EP, sty^Scer\UAS.cHa has eye phenotype, suppressible by Fas2^EB112/Fas2[+] (Mao and Freeman, 2009)
Suppressor of
Statement Reference sty^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 is a suppressor \| partially of wing phenotype of Scer\GAL4^Bx-MS1096, btl::Egfr^{Scer\UAS.T:λ\cI-DD} (Reich et al., 1999) sty^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 is a suppressor \| partially of wing vein phenotype of Scer\GAL4^Bx-MS1096, btl::Egfr^{Scer\UAS.T:λ\cI-DD} (Reich et al., 1999) sty^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 is a suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, htl^{Scer\UAS.T:λ\cI-DD} (Reich et al., 1999)
Other
Statement Reference Scer\GAL4^71B, phl^{Scer\UAS.F179}, sty^Scer\UAS.cHa has wing vein phenotype (Reich et al., 1999, Reich et al., 1999)
Additional Comments
Genetic Interactions
Statement Reference The rough-eye phenotype observed in flies expressing sty[Scer\UAS.cHa] under the control of Scer\GAL4[sev.EP] is suppressed in the presence of Fas2[EB112]/+. (Mao and Freeman, 2009) When expression is driven by Scer\GAL4^Bx-MS1096 in combination with btl::Egfr^{Scer\UAS.T:λ\cI-DD}, the wing size of the btl::Egfr^{Scer\UAS.T:λ\cI-DD} is largely normal and the wing veins, though still slightly thickened compared to wild type, are largely restored. When expression is driven by Scer\GAL4^Bx-MS1096 in combination with htl^{Scer\UAS.T:λ\cI-DD}, the wing vein defects caused by htl^{Scer\UAS.T:λ\cI-DD} are largely suppressed. Lethality of phl^{Scer\UAS.F179}, Scer\GAL4^71B is rescued by sty^Scer\UAS.cHa. Wings of phl^{Scer\UAS.F179}, sty^Scer\UAS.cHa, Scer\GAL4^71B mutants are largely devoid of wing vein. (Reich et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	sty^Scer\UAS.cHa sty^UAS.cHa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Hacohen (Hacohen et al., 1998)
Secondary FlyBase IDs

References ( 10 )
Research paper	Mao and Freeman, 2009, Development 136(3): 473--481 Fasciclin 2, the Drosophila orthologue of neural cell-adhesion molecule, inhibits EGF receptor signalling. [FBrf0206540] Jarvis et al., 2006, Development 133(6): 1133--1142 Sprouty proteins are in vivo targets of Corkscrew/SHP-2 tyrosine phosphatases. [FBrf0190302] Dutta et al., 2005, PLoS Biol. 3(10): e337 Drosophila Heartless acts with Heartbroken/Dof in muscle founder differentiation. [FBrf0191368] Iwanami et al., 2005, Genes Cells 10(7): 743--752 Cell-type specific utilization of multiple negative feedback loops generates developmental constancy. [FBrf0187603] Bai et al., 2001, Development 128(4): 591--601 The cell adhesion molecule Echinoid defines a new pathway that antagonizes the Drosophila EGF receptor signaling pathway. [FBrf0134572] Kumar and Moses, 2001, Development 128(14): 2689--2697 The EGF receptor and notch signaling pathways control the initiation of the morphogenetic furrow during Drosophila eye development. [FBrf0138358] Kumar and Moses, 2001, Cell 104(5): 687--697 EGF receptor and Notch signaling act upstream of Eyeless/Pax6 to control eye specification. [FBrf0134490] Kramer et al., 1999, Development 126(11): 2515--2525 Sprouty: a common antagonist of FGF and EGF signaling pathways in Drosophila. [FBrf0108521] Reich et al., 1999, Development 126(18): 4139--4147 Sprouty is a general inhibitor of receptor tyrosine kinase signaling. [FBrf0111472] Hacohen et al., 1998, Cell 92(2): 253--263 sprouty encodes a novel antagonist of FGF signaling that patterns apical branching of the Drosophila airways. [FBrf0100567]

Allele Dmel\styScer\UAS.cHa

Allele Dmel\sty^Scer\UAS.cHa