FlyBase Allele Report: Dmel\sty[Δ5]

Eggs derived from egg chambers containing complete homozygous follicle cell clones have an expanded dorsal midline region, with the dorsal appendages being further apart than normal. In addition, most of the appendages are mildly shorter in length or thicker in width than normal, some have branched tips, and rarely, a small pseudo-appendage is seen at the base of one of the appendages.

(Lachance et al., 2009)

Eggs derived from females with mosaic sty^Δ5 egg chambers occasionally have multiple, ectopic dorsal appendages. More commonly, the eggs have thinner dorsal appendages that are located more laterally than normal.

(Zartman et al., 2009)

sty^Δ5 larva show extra terminal cells in their tracheal systems, when compared to wild-type larva.

(Jarvis et al., 2006)

sty^Δ5 homozygous clones in the dorsal air sac primordium are found at the tip of the primordium at a significantly lower frequency than wild-type clones.

(Cabernard and Affolter, 2005)

Homozygotes have a variable number of outer photoreceptor neurons per ommatidium; some ommatidia have more and some have fewer than the normal number of outer photoreceptor neurons, and although the average number is 6.01 per ommatidium in the mutant (almost the same as wild type), the standard deviation is large (1.05).

(Iwanami et al., 2005)

When mutant somatic clones are made in the border cells no effect is seen.

(Duchek et al., 2001)

Homozygous eggs are not recovered from females containing homozygous germ line clones, suggesting a requirement for sty function in the germline.

(Casci et al., 1999)

Escapers of lethality show a disorganized eye phenotype. Majority of ommatidia have extra R7 cells, generated by transformation of cone cells. Some of the extra photoreceptors resemble outer (R1-R6) photoreceptor neurons. Mystery cells assume R3/R4 fates. In heterozygous condition, 1-7% of ommatidia show an extra R7 cell or an occasional gain or loss of an outer photoreceptor cell. In embryos, 26% of hemisegments show an extra chordotonal organ in the lateral cluster. Midline glial cells are increased in number.

(Kramer et al., 1999)

When clones are induced in the ovary, or specifically in the follicle cells, a range of phenotypes occurs, depending on the size of the clone. Eggs may be dorsalized, dorsal appendages may be misplaced or enlarged, and, rarely, dorsal appendages may be duplicated or split.

(Reich et al., 1999)

Pupal lethal when hemizygous. 30-120% more tracheal branches than normal bud from the dorsal, lateral trunk anterior, lateral trunk posterior, ganglionic and visceral branches. These additional branches arise from the stalks of the primary branches, close to the positions where secondary and terminal branches normally bud. The total number of tracheal cells is not increased in these embryos. In homozygous clones in which only the tracheal tip cell GB1 is sty^-, there is always an ectopic branch in the neighbouring sty⁺ stalk cell, GB2. These ectopic branches usually turn away from the ventral nerve cord, and growth of the normal branch from the sty^- GB1 cell may be slowed. In contrast, in clones in which a subset of stalk cells (GB2-7) are sty^- and GB1 is sty⁺, the ganglionic branch generally appears normal, with no ectopic branches. In clones in which the tracheal tip cell DB1 is sty^-, nearby sty⁺ cells form ectopic branches.

(Hacohen et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sty[+]/sty^Δ5 is an enhancer of visible phenotype of Chmp1^GD11219, Scer\GAL4^Bx-MS1096

(Valentine et al., 2014)

sty^Δ5 is an enhancer of abnormal cell polarity phenotype of fz²⁰/fz¹⁹

(Strutt and Strutt, 2003)

sty^Δ5 is an enhancer of visible | dominant phenotype of Kr^If-1

(Abrell et al., 2000)

Suppressor of

Statement

Reference

sty^Δ5 is a suppressor of abnormal cell polarity phenotype of Ras64B^V14.Act5C

(Strutt and Strutt, 2003)

sty^Δ5 is a suppressor of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

sty^Δ5 is a suppressor of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

NOT Suppressor of

Statement

Reference

sty[+]/sty^Δ5 is a non-suppressor of abnormal size | adult stage phenotype of Pp2B-14D^act.UAS.EYFP, Scer\GAL4^tin.CΔ4

(Lee et al., 2014)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

sty^Δ5 has phenotype, non-suppressible by aos^GMR.PS

(Kramer et al., 1999)

Enhancer of

Statement

Reference

sty[+]/sty^Δ5 is an enhancer of wing vein phenotype of Chmp1^GD11219, Scer\GAL4^Bx-MS1096

(Valentine et al., 2014)

sty[+]/sty^Δ5 is an enhancer of wing vein | ectopic phenotype of Hsap\ATN1^N917.65Q.UAS, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Charroux et al., 2006)

sty[+]/sty^Δ5 is an enhancer of photoreceptor cell R7 | increased number phenotype of csw::Src64B^hs.2sev

(Jarvis et al., 2006)

sty^Δ5 is an enhancer of ommatidium phenotype of fz²⁰/fz¹⁹

(Strutt and Strutt, 2003)

sty^Δ5 is an enhancer of eye phenotype of Kr^If-1

(Abrell et al., 2000)

sty^Δ5 is an enhancer of ommatidium phenotype of Kr^If-1

(Abrell et al., 2000)

Suppressor of

Statement

Reference

sty[+]/sty^Δ5 is a suppressor | partially of adult heart phenotype of Pp2B-14D^act.UAS.EYFP, Scer\GAL4^tin.CΔ4

(Lee et al., 2014)

sty[+]/sty^Δ5 is a suppressor of eye phenotype of rau^f04153/Df(2L)rau^Δ

(Sieglitz et al., 2013)

sty^Δ5 is a suppressor of eye photoreceptor cell | somatic clone phenotype of mop^T612

(Miura et al., 2008)

sty[+]/sty^Δ5 is a suppressor | partially of outer photoreceptor cell phenotype of csw^{G547E.hs.2sev}

(Jarvis et al., 2006)

sty^Δ5 is a suppressor of ommatidium phenotype of Ras64B^V14.Act5C

(Strutt and Strutt, 2003)

sty^Δ5 is a suppressor of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

sty^Δ5 is a suppressor of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

sty^Δ5 is a suppressor of phenotype of aos^GMR.PS

(Kramer et al., 1999)

NOT Suppressor of

Statement

Reference

sty[+]/sty^Δ5 is a non-suppressor of outer photoreceptor cell phenotype of csw^CS.hs.2sev

(Jarvis et al., 2006)

Other

Statement

Reference

aos^Δ7, sty^Δ5 has ommatidial precursor cluster | somatic clone phenotype

(Brown et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

sty^Δ5/+ rescues the reduced fractional shortening phenotype but not the cardiac enlargement of flies expressing Pp2B-14D^{act.Scer\UAS.T:Avic\GFP-EYFP} under the control of Scer\GAL4^tin.CΔ4.

(Lee et al., 2014)

sty^Δ5/+ suppresses the rough eye of Df(2L)rau^Δ adults and the 'R7 cell loss' phenotype seen in Df(2L)rau^Δ eye discs.

(Sieglitz et al., 2013)

The loss of photoreceptor cells seen in homozygous mop^T612 clones in the eye disc is rescued if the clones are also homozygous for sty^Δ5.

(Miura et al., 2008)

argos^Δ7 sty^Δ5 Minute clones in the eye disc form large, disorganised clusters in the morphogenetic furrow compared to the clusters formed in wild-type eye discs.

(Brown et al., 2006)

Flies that express one copy of csw::Src64B^hs.2sev in developing eyes with a sty^Δ5/+ background show an enhancement of the extra R7 photoreceptor cell phenotype seen when csw::Src64B^hs.2sev is expressed in a wild-type background. A higher proportion of the csw::Src64B^hs.2sev/+; sty^Δ5/+ flies show 3, 4, 5,or 6 R7 photoreceptor cells per ommatidium.

(Jarvis et al., 2006)

The addition of sty^Δ5/+ enhances the eye cell polarity phenotype seen in fz¹⁹/fz²⁰ animals. Heterozygotes suppress the ommatidial polarity defects seen in Ras64B^V14.Act5C.

(Strutt and Strutt, 2003)

Weakly suppresses the eye phenotype produced by activated arm constructs. (either arm^S44Y.GMR or arm^S56F.GMR).

(Freeman and Bienz, 2001)

Eyes mutant for both sty^Δ5 and carrying argos^GMR.PS have a normal-size eye and an extra-R7 phenotype similar to that of sty^Δ5 alone.

(Kramer et al., 1999)

Xenogenetic Interactions

Statement

Reference

The ectopic wing vein phenotype seen when Hsap\ATN1^{N917.65Q.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF is enhanced in sty^Δ5 heterozygotes.

(Charroux et al., 2006)

Complementation and Rescue Data

Fails to complement

sty⁵¹⁸

(Sullivan and Rubin, 2002)

sty^L1

(Abrell et al., 2000)

sty^S73

(Casci et al., 1999)

sty^S88

(Casci et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6382

w^*; sty^Δ5/TM3, Sb¹ P{35UZ}2

Notes on Origin

Discoverer

Comments

Amorph or strong hypomorph.

(Kramer et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Sty^Δ5

(Duchek et al., 2001)

sprouty^Δ5

(Freeman and Bienz, 2001)

spry^D5

(Cabernard and Affolter, 2005)

spry^Δ5

(Jarvis et al., 2006, Iwanami et al., 2005, Kramer et al., 1999, Reich et al., 1999, Hacohen et al., 1998)

sty^D5

(Rhiner et al., 2009, Sullivan and Rubin, 2002)

sty^Δ5

(Dollar et al., 2016, Lee et al., 2014, Valentine et al., 2014, Fregoso Lomas et al., 2013, Sieglitz et al., 2013, Sen et al., 2011, Lachance et al., 2009, Rhiner et al., 2009, Zartman et al., 2009, Miura et al., 2008, Brown et al., 2006, Charroux et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (26)

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