Over-expression using Scer\GAL4fkh.PH leads to a high proportion of salivary glands losing a ductal connection to the embryo surface.
Overexpression of argosScer\UAS.cHa, under the control of Scer\GAL4en-e16E in the posterior compartment of the wing leads to 100% of vein loss in vein L4, and 90% in vein L5.
Expression of argosScer\UAS.cHa, under the control of Scer\GAL4en-e16E, in embryonic posterior compartment cells leads to induction of apoptosis and reduction in compartment cell number. Surviving cells are larger than normal.
Stage 16 argosScer\UAS.cHa; Scer\GAL4en-e16E embryos have reduced numbers of oenocytes per cluster. Most clusters have 3 oenocytes, but a few have the wild-type 6, suggesting that one round of delamination of 3 oenocytes occurs as in wild-type but that a second round is frequently blocked.
Expression of aosScer\UAS.cHa under the control of Scer\GAL469B results in a narrower wing in which vein L4 and the anterior crossvein are missing.
When argosScer\UAS.cHa is driven by Scer\GAL4sca-537.4 27% of bracts in the tibia and 85% in the basitarsus of the second leg remain. Animals have fewer bracts in dorsal vs ventral rows on the basitarsus. The dorsal rows 4 and 5 have 27% and 44% of bracts respectively, compared to 85-100% for the remaining rows. Also in 3/10 legs a transformation of the huge 'apical' bristle into a chemosensory bristle of ordinary size is seen. When driven by Scer\GAL4Dll-md23 26% of bracts in the tibia and 30% in the basitarsus of the second leg remain. Animals have fewer bracts in dorsal vs ventral rows on the basitarsus of the second leg. Mutant legs also lack claws and apodemes. Also in 4/10 legs a transformation of the huge 'apical' bristle into a chemosensory bristle of ordinary size is seen.
When expression is driven by Scer\GAL4sca-C253, both macrochaetae and microchatea are suppressed on the notum.
Wing veins develop normally when argosScer\UAS.cHa is expressed under the control of Scer\GAL4604 and in some cases ectopic wing vein tissue is formed, in close proximity to vein territories.
Scer\GAL469B-mediated expression causes a mild or moderate extra-vein phenotype. Winga ares also smaller than wild type as vein cells that replace inter vein cells are more compact. Mediated expression also causes a rough eye phenotype: eyes are smaller than wild type and some ommatidia are fused. Scer\GAL4bs-1348-mediated expression causes a mild or moderate extra-vein phenotype.