cswlf hemizygous adult males display a rough eye phenotype.
cswlf/Y flies show loss of wing vein material.
Homozygous females and hemizygous males have small, rough eyes. Wing veins L2 and L5 do not reach the wing margin.
Homozygotes have a reproducible rough eye and wing vein gap phenotype. Stronger phenotypes are seen at higher temperatures. The rough eye phenotype results from a loss of photoreceptors, commonly R7, although there is also occasional loss of outer photoreceptors. The wing vein gaps are most frequently seen in the distal regions of L5 and L2, and occasional gaps are seen in L4. Homozygous females are essentially sterile and only rarely produce fertilised eggs. The eggs from homozygous females have shells that are partially ventralised; the dorsal appendages are partially fused. Embryos derived from homozygous females fail to hatch. Embryos derived from a cross of females containing homozygous germline clones to hemizygous males fail to hatch. The cuticles of these embryos are largely wild type, although mild defects such as partial fusion of denticle bands along the midline, mild twisting and incomplete germ band shortening are frequently seen. The eve-expressing DA1/#1 and pericardial cell precursors are variably missing. The VA2#27 muscle precursor is also variably missing. Embryos show either relatively little tracheal disruption (such as interruption of the dorsal trunk) or significant disruption where the dorsal trunk is interrupted one or more times along the anteroposterior axis and the dorsoventral tracheal branch pattern is misrouted.
Rough eye and vein gap phenotypes.