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General Information
Symbol
Dmel\commUAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kidd
FlyBase ID
FBal0086681
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-comm
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 1.7kb XhoI-XbaI comm cDNA fragment is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of commScer\UAS.cKa under the control of Scer\GAL4Pdf.PU does not significantly affect the length of sLNv axons compared to controls.

Expression of commScer\UAS.cKa under the control of Scer\GAL4elav-C155 results in a "roundabout" phenotype of longitudinal axons crossing the midline in embryos.

Down-regulation of robo through targeted expression of commScer\UAS.cKa under the control of Scer\GAL4eve.CQ2 induces ectopic dendritic innervation of the midline neuropile (ith 100% penetrance for MN-LL1 and 62.5% penetrance for MN-DA3) with a concomitant reduction of the lateral arbor.

The antennal lobes of commScer\UAS.cKa; Scer\GAL4SG18.1 flies have a disorganized pattern of glomeruli, and while the antennal commissure is present, its axons are loosely fasciculated.

When expression is driven by Scer\GAL412.1 the cd sensory neuron projections are shifted from the intermediate to the medial longitudinal fascicles. In approximately 50% of cases, cd sensory neuron projections cross the midline.

Longitudinal tracts look normal in embryos expressing commScer\UAS.cKa under the control of Scer\GAL4btl.PS. 17% of ganglionic branches cross the midline in these embryos, 14% turn anteriorly at the midline and 15% stall prematurely.

Expression of commScer\UAS.cKa under the regulation of Scer\GAL4A307, in the giant fiber system results in the collapse of the giant fiber axons at the midline, with the axons often wrapping around each other. The latency (a measure of synaptic function) of these axons is unaffected. Expression of commScer\UAS.cKa in the tergotrochanteral motor neurons, under the control of Scer\GAL4shakB.lethal.4.1 has no effect on the dendritic projection or the function of the synapse.

Expression of commScer\UAS.cKa under the control of Scer\GAL4ap-md52 causes ap-expressing neurons to cross the midline, in contrast to wild type. Thoracic-specific ap-expressing neurons cross in the posterior commissure (PC), whereas other ap-expressing neurons cross in the anterior commissure (AC).

Axons freely cross and recross the midline in the central nervous system (CNS) of embryos expressing commScer\UAS.cKa under the control of Scer\GAL4sca-537.4. Increasing the copy number of commScer\UAS.cKa results in a more severe phenotype, the strongest phenotype has axons entering the midline but not leaving it, leading to a collapse of the CNS scaffold onto the midline.

More axons than normal cross the midline in embryos expressing commScer\UAS.cKa under the control of Scer\GAL4sca-537.4. The pCC pathway crosses the midline, joins with its contralateral pathway and forms circles around the midline. The midline glia are present and migrate to their normal position. The phenotype caused by commScer\UAS.cKa expressed under the control of Scer\GAL4sca-537.4 is dosage sensitive.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Suppressor of
Other
Statement
Reference

Scer\GAL4ap-md52, commUAS.cKa, drlUAS.cCa has embryonic ventral nervous system & embryonic thoracic segment phenotype

Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Rab5S43N.Scer\UAS.P\T.T:Avic\GFP-YFP has no effect on the "roundabout" phenotype (longitudinal axons crossing the midline) in embryos expressing commScer\UAS.cKa under the control of Scer\GAL4elav-C155.

Expression of commScer\UAS.cKa under the control of Scer\GAL4eg-Mz360 partially suppresses the EW axon guidance defects in fraunspecified mutants.

Overexpression of commScer\UAS.cKa under the control of Scer\GAL4eg-Mz360 in Df(1)NetABΔ mutants does not affect the guidance defects seen in Df(1)NetABΔ mutant embryos.

The lateral shifts in the giant fibers induced by overexpression of roboScer\UAS.cKa or leaScer\UAS.cSa, under the control of Scer\GAL4A307 can be suppressed by co-overexpression of commScer\UAS.cKa with Scer\GAL4A307. When commScer\UAS.cKa is coexpressed with roboScer\UAS.cKa or leaScer\UAS.cSa, the lateral displacement of the giant fiber axons is completely rescued. When commScer\UAS.cKa is coexpressed with roboScer\UAS.cKa, under the control of Scer\GAL4shakB.lethal.4.1, one copy of commScer\UAS.cKa is able to suppress the anatomical and physiological effect of one or two copies of roboScer\UAS.cKa.

When both commScer\UAS.cKa and fra::roboScer\UAS.FR.T:Hsap\MYC are both expressed in all neurons by Scer\GAL4elav.PLu, suppression of the fra::roboScer\UAS.FR.T:Hsap\MYC phenotype is seen with some significant commissure formation in greater than 60% of segments (n+29 animals).

Co-expression of drlScer\UAS.cCa under the control of Scer\GAL4ap-md52 switches the projections of all thoracic-specific ap-expressing neurons from the PC to the AC, whereas co-expression of drlΔE.Scer\UAS has no effect.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
commScer\UAS.cKa
commUAS.cKa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Kidd
Secondary FlyBase IDs
    References (14)