Allele Dmel\cas^Scer\UAS.cKa

General Information
Symbol	Dmel\cas^Scer\UAS.cKa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Kambadur	FlyBase ID	FBal0086694
Feature type	allele	Associated gene	Dmel\cas

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Kambadur et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: cas cDNA containing the open reading frame is cloned into the pUAST vector. (Kambadur et al., 1998)
Carried in construct	P{UAS-cas.K} (Kambadur et al., 1998, Grosskortenhaus et al., 2006, Maurange et al., 2008, Baumgardt et al., 2009, Losada-Pérez et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| embryonic stage, with Scer\GAL4^en-e16E (Grosskortenhaus et al., 2006) neuroanatomy defective \| embryonic stage, with Scer\GAL4^pros.PMG (Grosskortenhaus et al., 2006)
Phenotype Manifest In
	subesophageal ganglion neuron, with Scer\GAL4^elav-C155 (Losada-Pérez et al., 2010) U4 neuron, with Scer\GAL4^en-e16E (Grosskortenhaus et al., 2006) U4 neuron, with Scer\GAL4^pros.PMG (Grosskortenhaus et al., 2006) U5 neuron, with Scer\GAL4^en-e16E (Grosskortenhaus et al., 2006) U5 neuron, with Scer\GAL4^pros.PMG (Grosskortenhaus et al., 2006)
Detailed Description
	Statement Reference Animals expressing cas[Scer\UAS.cKa] under the control of Scer\GAL4[elav-C155] have an increase in the number of SELK neurons compared to control animals. (Losada-Pérez et al., 2010) Embryos expressing cas^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E lack U4 and U5 neurons, although U1-U3 neuron fates are normal. Embryos expressing cas^Scer\UAS.cKa under the control of Scer\GAL4^pros.PMG lack U4 and U5 neurons. (Grosskortenhaus et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4^elav-C155/cas^Scer\UAS.cKa is a suppressor of increased cell death phenotype of Scer\GAL4^elav-C155, abd-A^{1dB5.Scer\UAS} (Maurange et al., 2008)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^elav-C155/cas^Scer\UAS.cKa is a suppressor of neuroblast phenotype of Scer\GAL4^elav-C155, abd-A^{1dB5.Scer\UAS} (Maurange et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference The neuroblast apoptosis seen in the thorax and abdomen of 96 hour larvae expressing abd-A[1dB5.Scer\UAS] under the control of Scer\GAL4[elav-C155] is suppressed by co-expression of cas[Scer\UAS.cKa]. (Maurange et al., 2008) Co-expression of cas^Scer\UAS.cKa and pdm2^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E results in embryos which have normal U1/U2 neurons, but show a loss of the U3/U4 neurons and extra U5 neurons. (Grosskortenhaus et al., 2006)
Xenogenetic Interactions
Statement Reference Co-expression of BacA\p35^Scer\UAS.cHa and cas^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E results in loss of U4/U5 neurons (as is seen in embryos expressing cas^Scer\UAS.cKa alone under the control of Scer\GAL4^en-e16E). (Grosskortenhaus et al., 2006)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	cas^Scer\UAS.cKa cas^UAS.cKa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Kambadur (Kambadur et al., 1998)
Secondary FlyBase IDs

References ( 5 )
Research paper	Losada-Pérez et al., 2010, Mech. Dev. 127(9-12): 458--471 Lineage-unrelated neurons generated in different temporal windows and expressing different combinatorial codes can converge in the activation of the same terminal differentiation gene. [FBrf0212045] Baumgardt et al., 2009, Cell 139(5): 969--982 Neuronal subtype specification within a lineage by opposing temporal feed-forward loops. [FBrf0209429] Maurange et al., 2008, Cell 133(5): 891--902 Temporal transcription factors and their targets schedule the end of neural proliferation in Drosophila. [FBrf0204899] Grosskortenhaus et al., 2006, Genes Dev. 20(18): 2618--2627 Pdm and Castor specify late-born motor neuron identity in the NB7-1 lineage. [FBrf0193400] Kambadur et al., 1998, Genes Dev. 12(2): 246--260 Regulation of POU genes by castor and hunchback establishes layered compartments in the Drosophila CNS. [FBrf0100094]

Allele Dmel\casScer\UAS.cKa

Allele Dmel\cas^Scer\UAS.cKa