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General Information
Symbol
Dmel\brmK804R.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0086705
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-brm[K804R], UAS-brmDN, UAS-brmK804R, brmK804R, UASBrmK804R
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 6.7kb fragment containing the brm coding sequence (with the amino acid replacement: K804R and tagged with Tag:HA), flanked by 66bp of 5' genomic DNA and approximately 1.3kb of 3' genomic DNA is expressed under the control of UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
ameliorates  cancer
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

macrochaeta & wing, with Scer\GAL469B

sensillum campaniformium & wing, with Scer\GAL469B

Detailed Description
Statement
Reference

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4C587 does not cause any obvious defects in germaria morphology and development.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey.PU results in small and rough eye phenotype in adult flies.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey.PH results in small eye phenotype in adults.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4wor.PA has no effect on neuroblast number.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 via the Scer\GAL4en-e16E driver leads to a loss of the L5 wing vein and loss of the posterior cross vein.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4hs.PB results in a slight increase of S-phase cells in third instar imaginal wing discs compared with controls. Expression of brmK804R.Scer\UAS.T:Ivir\HA1 has little effect on mitosis or cell survival.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.G32 does not alter the morphology of mitotic chromosomes prepared from neuroblasts of third instar larvae.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey.PH results in flies with eyes that are slightly rough and reduced in size. The severity of the phenotype is enhanced if the flies also carry brm2, brm3369 or Df(3L)brm11. Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4neur-GAL4-A101 results in high embryonic or larval lethality (78%), early pupal lethality (17%) and midpupal lethality (5%). Pupae expressing brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4neur-GAL4-A101 contain variable-sized patches of tissue containing wild-type or defective sensory organ precursor (SOP) lineages. The defective SOP lineages contain fewer socket cells, fewer sheath cells and extra neurons.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL469B at 18oC results in the loss of distal portions of wing vein L5 and deletion of the posterior crossvein.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey.PH results in a rough eye phenotype.

brmK804R.Scer\UAS.T:Ivir\HA1 when driven by Scer\GAL4ey.PH produces flies with slightly smaller or rough eyes. A small percentage of these flies die as pharate adults.

When expression is driven by Scer\GAL4bi-omb-Gal4, loss of the distal wing margin, formation of ectopic campaniform sensillae and wing margin bristles and disruptions of wing vein morphology are seen. Expression of brmK804R.Scer\UAS.T:Ivir\HA1 at the wing margin driven by Scer\GAL4vg.PM, results in a loss the proximal, posterior wing margin.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4h-1J3 or Scer\GAL4en-e16E has no effect on embryonic viability or segmentation. Expression of brmK804R.Scer\UAS.T:Ivir\HA1 in imaginal tissues under the control of Scer\GAL469B causes greater than 99% lethality. At 25oC or higher, the majority of these animals pupate but fail to differentiate. Some flies expressing brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL469B can survive to adulthood when raised at 20oC. These animals show partial transformation of first leg to second leg, as shown by a reduction in the number of sex comb teeth. These flies also show twinning of mechanosensory bristles. Wings are reduced in size, and the posterior crossvein and vein L5 are usually missing. Defects in the wing campaniform sensilla are also seen, including missing sensilla, duplication or triplication of sensilla, transformation of sensilla into bristles and the appearance of ectopic sensilla. Ectopic sensilla and bristles are most often seen on the L3 vein.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4ey.PH, brmK804R.UAS.Tag:HA has eye phenotype, enhanceable by Snr1R3/Snr1[+]

Scer\GAL4ey.PH, brmK804R.UAS.Tag:HA has eye phenotype, enhanceable by Dl9P/Dl[+]

Scer\GAL4ey.PH, brmK804R.UAS.Tag:HA has eye phenotype, enhanceable by RpII140[+]/RpII140A5

Scer\GAL4ey.PH, brmK804R.UAS.Tag:HA has eye phenotype, enhanceable by RpII140[+]/RpII140brie

Scer\GAL4ey.PH, brmK804R.UAS.Tag:HA has eye phenotype, enhanceable by vn[+]/vn643

Scer\GAL4ey.PH, brmK804R.UAS.Tag:HA has eye phenotype, enhanceable by zen2/zen[+]

Scer\GAL4ey.PH, brmK804R.UAS.Tag:HA has eye phenotype, enhanceable by zen436/zen[+]

Scer\GAL4ey.PH, brmK804R.UAS.Tag:HA has eye phenotype, enhanceable by zen714/zen[+]

NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The tumorigenic accumulation of germline stem cell-like cells in germaria, characteristic for females expressing ph-pGD17398 under the control of Scer\GAL4C587 (together with tub-Gal80[ts] to limit the time of expression to adulthood) is strongly suppressed by co-expression of brmK804R.Scer\UAS.T:Ivir\HA1.

The small and rough eye phenotype observed in adult flies expressing brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey.PU is exacerbated further by Marcal1del homozygosity.

The co-expression of brmK804R.Scer\UAS.T:Ivir\HA1 and scribHMS01490 under the control of Scer\GAL4Bx-MS1096 is synthetic lethal.

The co-expression of brmK804R.Scer\UAS.T:Ivir\HA1 suppresses the tumor phenotype and tissue disorganization resulting from the expression of dlg1HMS01954 under the control of Scer\GAL4Bx-MS1096.

The small adult eye phenotype induced by expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey.PH is further worsened by co-expression of httGD14943.

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of an insertion of Scer\GAL4GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2) enhances the supernumerary neuroblast phenotype seen in bratDG19310/brat11 mutant larval brains. Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4wor.PA also enhances the phenotype. No enhancement is seen when brmK804R.Scer\UAS.T:Ivir\HA1 is expressed using an insertion of Scer\GAL4GMR9D11 that expresses in ase[+] INPs (P{GMR9D11-GAL4}attP2).

Expression of brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4wor.PA enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm1/Df(2L)erm2 mutants expressing ermT:Zzzz\FLAG).

Co-expression of brmK804R.Scer\UAS.T:Ivir\HA1 and gemininScer\UAS.cQa driven by Scer\GAL4en-e16E causes a dramatic developmental defect leading to lethality during the first instar larval stage.

Co-expression of brmK804R.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4hs.PB does not enhance the S-phase, mitotic and cell death phenotypes of gemininScer\UAS.cQa expression.

Co-expression of brmK804R.Scer\UAS.T:Ivir\HA1 with phlScer\UAS.F179 via Scer\GAL4bbg-C96 does not significantly affect the notched wing phenotype resulting from the expression of phlScer\UAS.F179 alone.

Co-expression of both brmK804R.Scer\UAS.T:Ivir\HA1 and gemininScer\UAS.cQa with phlScer\UAS.F179 via Scer\GAL4bbg-C96 results in a significant suppression of the notched wing phenotype resulting from the expression of phlScer\UAS.F179 alone.

The loss of wing vein L5 and the posterior crossvein seen in animals expressing brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL469B at 18oC is suppressed by Snr1E1/+. The loss of wing veins seen in animals expressing brmK804R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL469B is suppressed by Snr1R3.

The rough eye phenotype of brmK804R.Scer\UAS.T:Ivir\HA1; Scer\GAL4ey.PH flies is enhanced by asf1Scer\UAS.cMa.

The majority of brmK804R.Scer\UAS.T:Ivir\HA1, Scer\GAL4ey.PH, Df(1)18.1.15 flies fail, to eclose with extremely rough, reduced or missing eyes. This enhancement is suppressible by the addition of dalaoScer\UAS.T:Ivir\HA1,T:Zzzz\His6.

The wing phenotypes caused by expression of brmK804R.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4bi-omb-Gal4 are strongly enhanced by the addition of osa308. The wing phenotypes caused by expression of brmK804R.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4vg.PM are enhanced by osa308. This phenotype is suppressed by the addition of osaScer\UAS.cCa. The addition of brmK804R.Scer\UAS.T:Ivir\HA1 suppresses the wing hinge and small wing size phenotypes seen in osaScer\UAS.cCa/Scer\GAL4vg.PM flies. The addition of brmK804R.Scer\UAS.T:Ivir\HA1 enhances the variable eye size phenotype seen in osaScer\UAS.cCa/Scer\GAL4ey.PH flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
brmK804R.Scer\UAS.T:Ivir\HA1
brmK804R.UAS.T:HA1
brmK804R.UAS.Tag:HA
Name Synonyms
Secondary FlyBase IDs
    References (23)