FlyBase Allele Report: Dmel\Pka-R1[BDK.UAS]

General Information

Symbol

Dmel\Pka-R1^BDK.UAS

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kalderon

FlyBase ID

FBal0086779

Feature type

allele

Associated gene

Dmel\Pka-R1

Associated Insertion(s)

Carried in Construct

P{UAS-Pka-R1.BDK}

Also Known As

UAS-R*, UAS-PKAinh¹, UAS-PKAr, UAS-PKAr*, UAS-PKA^DN, UAS-PKA-R1.BDK

Key Links

Transgenic product class

dominant_negative_variant

(Wolfgang et al., 1996)

missense_variant

(Kalderon, 1998.3.12)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Wolfgang et al., 1996)

missense_variant

(Kalderon, 1998.3.12)

Mutagen

in vitro construct

(Davis et al., 1998, Kalderon, 1998.3.12, Ohlmeyer and Kalderon, 1997, Li et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-Pka-R1.BDK}

Cytology

Description

UAS regulatory sequences drive expression of a mutant form of Pka-R1 that contains mutations in two cAMP binding sites (amino acid replacements G196E and G321D). This mutant regulatory subunit acts as an inhibitor of protein kinase A (PKA) activity; the mutations slightly reduce affinity for the catalytic subunit in vitro and makes the regulatory subunit entirely insensitive to cAMP.

(Kalderon, 1998.3.12)

Encodes a dominant negative regulatory form of Pka-R1.

(Wolfgang et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pka-R1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-208.UAS}

(Gonzales et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Wu et al., 2009)

abnormal courtship behavior | female, with Scer\GAL4^fru.P1.D

(Yang et al., 2009)

abnormal developmental rate, with Scer\GAL4^{Itpr-1664GAL4}

(Venkatesh et al., 2001)

abnormal feeding behavior | adult stage, with Scer\GAL4^sNPF-R.2.5

(Hong et al., 2012)

abnormal mating behavior | female, with Scer\GAL4^fru.P1.D

(Yang et al., 2009)

abnormal neurophysiology, with Scer\GAL4^eve.RN2

(Baines, 2004)

abnormal neurophysiology | larval stage, with Scer\GAL4^Mhc.PW

(Davis et al., 1998)

abnormal thermotaxis | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Hong et al., 2008)

chemical resistant, with Scer\GAL4^NPF.1

(Chen et al., 2008)

increased sleep | adult stage, with Scer\GAL4^ey-OK107

(Machado Almeida et al., 2021)

lethal, with Scer\GAL4^h-1J3

(Kiger et al., 1999)

lethal, with Scer\GAL4^JW1

(Kiger et al., 1999)

lethal, with Scer\GAL4^PP3

(Kiger et al., 1999)

lethal - all die before end of larval stage, with Scer\GAL4^e22c

(Kiger et al., 1999)

lethal - all die before end of P-stage, with Scer\GAL4^dpp.blk1

(Guichard et al., 2006)

partially lethal, with Scer\GAL4^KO5

(Kiger et al., 1999)

partially lethal, with Scer\GAL4^RK5

(Kiger et al., 1999)

some die during larval stage, with Scer\GAL4^e22c

(Kiger et al., 1999)

some die during pharate adult stage, with Scer\GAL4^dpp.blk1

(Guichard et al., 2006)

viable, with Scer\GAL4^RZ4

(Kiger et al., 1999)

visible, with Scer\GAL4^RZ4

(Kiger et al., 1999)

wild-type, with Scer\GAL4^GMR.PF

(Cho et al., 2001)

Phenotype Manifest In

adult, with Scer\GAL4^RZ4

(Kiger et al., 1999)

adult abdomen & cuticle, with Scer\GAL4^RK5

(Kiger et al., 1999)

adult cuticle, with Scer\GAL4^KO5

(Kiger et al., 1999)

adult thorax & cuticle, with Scer\GAL4^RK5

(Kiger et al., 1999)

cuticle, with Scer\GAL4^E62-2

(Li et al., 1995)

denticle belt & abdominal segment, with Scer\GAL4^e22c

(Kiger et al., 1999)

denticle belt & thoracic segment, with Scer\GAL4^e22c

(Kiger et al., 1999)

larva, with Scer\GAL4^RZ4

(Kiger et al., 1999)

leg, with Scer\GAL4^dpp.blk1

(Guichard et al., 2006)

leg, with Scer\GAL4^h-1J3

(Kiger et al., 1999)

leg sensillum | ectopic, with Scer\GAL4^h-1J3

(Kiger et al., 1999)

marginal wing cell | anterior, with Scer\GAL4^RZ4

(Kiger et al., 1999)

metathoracic metatarsus, with Scer\GAL4^C166-2

(Li et al., 1995)

metathoracic metatarsus, with Scer\GAL4^h-1J3

(Li et al., 1995)

oocyte | germline clone, with Scer\GAL4^{VP16.mat.αTub67C}

(Baum et al., 2000)

scutellar bristle, with Scer\GAL4^KO5

(Kiger et al., 1999)

scutellar bristle, with Scer\GAL4^RK5

(Kiger et al., 1999)

ventral denticle belt | posterior, with Scer\GAL4^e22c

(Ohlmeyer and Kalderon, 1997)

wing, with Scer\GAL4^C166-2

(Li et al., 1995)

wing, with Scer\GAL4^dpp.blk1

(Guichard et al., 2006)

wing, with Scer\GAL4^E62-2

(Li et al., 1995)

wing, with Scer\GAL4^h-1J3

(Li et al., 1995)

wing, with Scer\GAL4^KO5

(Kiger et al., 1999)

wing, with Scer\GAL4^RK5

(Kiger et al., 1999)

wing, with Scer\GAL4^RZ4

(Kiger et al., 1999)

wing blade anterior compartment | proximal, with Scer\GAL4^30A

(Wolfgang et al., 1996)

wing disc, with Scer\GAL4^ap-md544

(Vervoort et al., 1999)

yolk | germline clone, with Scer\GAL4^{VP16.mat.αTub67C}

(Baum et al., 2000)

yolk granule | germline clone, with Scer\GAL4^{VP16.mat.αTub67C}

(Baum et al., 2000)

Detailed Description

Statement

Reference

Flies expressing Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^sNPF-R.2.5 show significantly decreased food consumption compared to controls.

(Hong et al., 2012)

Expression of Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} (in the presence of RU486) results in an increase in sleep.

Expression of Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} (in the presence of RU486) completely suppresses the effects of caffeine on sleep.

Expression of Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^{Mef2.247.Switch} (in the presence of RU486) has no effect on the effects of caffeine on sleep.

(Wu et al., 2009)

Virgin females expressing Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^fru.P1.D show reduced sexual receptivity and an increased egg-laying rate compared to control virgin females. The mutant virgin females show rejection behaviours towards courting males.

(Yang et al., 2009)

Flies expressing Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^npf.1 are significantly more resistant to ethanol intoxication relative to paired controls. These flies develop normal alcohol tolerance. The ethanol levels in these flies immediately after exposure to ethanol vapor is the same as in controls, indicating that the flies have similar absorption rates. Further, the turnover rates of ethanol in these flies is the same as controls.

(Chen et al., 2008)

After 24 hours of feeding with RU486, flies expressing Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^{elav.Switch.PO} show a decreased avoidance of low temperature, but not high temperature.

Flies expressing Pka-R1^BDK.Scer\UAS under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c747 (restricted to 16 hours expression from day three of adulthood onwards using Scer\GAL80^ts.αTub84B) show decreased avoidance of low temperatures.

(Hong et al., 2008)

Expression of Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^dpp.blk1 results in lethality during late pupariation. Excavation of these adults shows severe wing and leg patterning defects.

(Guichard et al., 2006)

Postsynaptic expression of Mmus\Pkaca^mC.Scer\UAS, under the control of Scer\GAL4^eve.RN2, results in the averaged amplitude of synaptic currents recorded from whole-cell voltage clamp recordings of aCC/RP2 motoneurons being significantly decreased compared with controls.

(Baines, 2004)

Many cells persist for much longer after eclosion in the posterior compartment of the wings of Pka-R1^BDK.Scer\UAS; Scer\GAL4^en-e16E adults than in wild-type. This results in separation between the dorsal and ventral cuticular sheets in the posterior compartment of the wings.

(Kimura et al., 2004)

Expression of Pka-R1^BDK.Scer\UAS, under the control of Scer\GAL4^eve.RN2O, in aCC/RP2 neurons does not affect action potential or inward Na⁺ current.

(Baines, 2003)

When Pka-R1^BDK.Scer\UAS is driven by Scer\GAL4^GMR.PF, no eye phenotype is seen.

(Cho et al., 2001)

When expression is driven by Scer\GAL4^{Itp-r83A-1664GAL4} larval molting is delayed, particularly at the transitions between instars and at the L3 to pupal and pupal to adult transitions. Lethality is not observed. Similar effects are seen when expression is driven by Scer\GAL4^hs.PB grown at 29^oC. Developmental delay can be rescued by feeding afflicted L2 larvae with 20-hydroxyecdysone.

(Venkatesh et al., 2001)

Pka-R1 germ-line clone oocytes lose both nurse cell cortical actin (leading to cell fusion and ectopic actin structures within the oocyte close to the nurse cell-oocyte interface. Pka-R1 germ-line clone oocytes exhibit mRNA polarity defects. Yolk also fails to form within the oocyte of many mutant egg chambers.

(Baum et al., 2000)

Expression of insertion P{UAS-Pka-R1.BDK}33 in Scer\GAL4^KO5 and Scer\GAL4^RK5 backgrounds allows partial survival; 22 with a sibling class of 123, and 14 with a sibling class of 117, respectively. With Scer\GAL4^RK5, small larvae feed and linger for up to 3 weeks before dying. With Scer\GAL4^RK5 or Scer\GAL4^KO5, those that survive to adulthood are all abnormal: the cuticle of the thorax and abdomen has an abnormal sheen, there is melanisation of the thorax and wings and there are crossed scutellar bristles. Expression of insertion P{UAS-Pka-R1.BDK}33 in Scer\GAL4^JW1, and Scer\GAL4^PP3 backgrounds show near complete lethality, acting during embryonic and young first instar larvae. Insertion P{UAS-Pka-R1.BDK}35 also shows partial survival in a Scer\GAL4^KO5 background; 4 with a sibling class of 84. P{UAS-Pka-R1.BDK}35 in Scer\GAL4^RK5, Scer\GAL4^JW1 and Scer\GAL4^PP3 backgrounds show complete or near complete lethality. P{UAS-Pka-R1.BDK}35 in the Scer\GAL4^RZ4 background produces adult flies with 51% having anterior wing duplications (62/122 have at least one wing affected). Those flies with normal wings are often reduced in size because of retarded larval growth. P{UAS-Pka-R1.BDK}35 in the Scer\GAL4^e22c background leads to early larval lethality, most larvae hatch and die within a few hours. Many of these larvae exhibit an absence of thoracic denticle belts, as well as a change in the pattern of abdominal denticle belts. This phenotype is partly suppressed by Pka-C1^{Scer\UAS.T:Zzzz\FLAG}, suppression being more evident for abdominal than for thoracic denticle belts. When expression is driven by Scer\GAL4^h-1J3 most adults fail to emerge from the pupal case and have truncated legs with multiple ectopic bristle columns.

(Kiger et al., 1999)

When driven by Scer\GAL4^ap-md544 leads to an outgrowth of the wing disc.

(Vervoort et al., 1999)

Expression of Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^Mhc.PW significantly increases the average spontaneous miniature excitatory junctional current amplitude in muscle 6 in larvae. There is a significant increase in the average compound excitatory junctional current at the neuromuscular junction in larvae expressing Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^Mhc.PW compared to wild-type larvae.

(Davis et al., 1998)

Embryos expressing Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^e22c give rise to larvae with abnormal denticle belts; the wide posterior denticle rows of each segment are replaced by narrower and often incomplete rows of denticles with different morphology and polarity from wild-type.

(Ohlmeyer and Kalderon, 1997)

Produces partial wing duplications in the most proximal portion of the anterior compartment of the wing, when expressed using Scer\GAL4^30A.

(Wolfgang et al., 1996)

Partial wing duplication, cuticular outgrowth, distally truncated tarsae with multiple ectopic bristle columns.

(Li et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pka-R1^BDK.UAS, Scer\GAL4^{Itpr-1664GAL4} has abnormal developmental rate phenotype, enhanceable by Itpr^1664GAL4/Itpr⁰⁵⁶¹⁶

(Venkatesh et al., 2001)

Suppressed by

Statement

Reference

Pka-R1^BDK.UAS has lethal | pharate adult stage phenotype, suppressible by Scer\GAL4^dpp.blk1/Bant\EF^UAS.cGa

(Guichard et al., 2006)

Pka-R1^BDK.UAS, Scer\GAL4^Mhc.PW has abnormal neurophysiology | larval stage phenotype, suppressible by Pde4^dnc-M14

(Davis et al., 1998)

NOT suppressed by

Statement

Reference

Pka-R1^BDK.UAS, Scer\GAL4^{Itpr-1664GAL4} has abnormal developmental rate phenotype, non-suppressible by Itpr^UAS.cVa, Scer\GAL4^{Itpr-1664GAL4}

(Venkatesh et al., 2001)

Suppressor of

Statement

Reference

Pka-R1^BDK.UAS, Scer\GAL4^elav.PU is a suppressor of abnormal sleep phenotype of Hsap\HTT^{128Q.1-208.UAS}, Scer\GAL4^elav.PU

(Gonzales et al., 2016)

Pka-R1^BDK.UAS, Scer\GAL4^elav-C155 is a suppressor of partially lethal - majority die phenotype of Hsap\HTT^{128Q.1-208.UAS}, Scer\GAL4^elav-C155

(Gonzales et al., 2016)

Pka-R1^BDK.UAS, Scer\GAL4^sNPF-R.2.5 is a suppressor of abnormal feeding behavior | adult stage phenotype of Scer\GAL4^sNPF-R.2.5, sNPF-R^UAS.cLa

(Hong et al., 2012)

Pka-R1^BDK.UAS/Scer\GAL4^eve.RN2O is a suppressor of abnormal neurophysiology | dominant phenotype of Ace^j50

(Baines, 2003)

Other

Statement

Reference

Itpr^90B.0/Itpr^1664GAL4, Pka-R1^BDK.UAS, Scer\GAL4^{Itpr-1664GAL4} has lethal phenotype

(Venkatesh et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pka-R1^BDK.UAS, Scer\GAL4^{VP16.mat.αTub67C} has oocyte | germline clone phenotype, enhanceable by capt^k01217

(Baum et al., 2000)

Suppressed by

Statement

Reference

Pka-R1^BDK.UAS has wing phenotype, suppressible by Scer\GAL4^dpp.blk1/Bant\EF^UAS.cGa

(Guichard et al., 2006)

Pka-R1^BDK.UAS has leg phenotype, suppressible by Scer\GAL4^dpp.blk1/Bant\EF^UAS.cGa

(Guichard et al., 2006)

Pka-R1^BDK.UAS, Scer\GAL4^RZ4 has wing phenotype, suppressible by Pka-C1^UAS.Tag:FLAG, Scer\GAL4^RZ4

(Kiger et al., 1999)

Pka-R1^BDK.UAS, Scer\GAL4^e22c has denticle belt & thoracic segment phenotype, suppressible | partially by Pka-C1^UAS.Tag:FLAG, Scer\GAL4^e22c

(Kiger et al., 1999)

Pka-R1^BDK.UAS, Scer\GAL4^e22c has denticle belt & abdominal segment phenotype, suppressible by Pka-C1^UAS.Tag:FLAG, Scer\GAL4^e22c

(Kiger et al., 1999)

NOT Enhancer of

Statement

Reference

Pka-R1^BDK.UAS, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Pdk1^EP837, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Suppressor of

Statement

Reference

Pka-R1^BDK.UAS is a suppressor of wing phenotype of Scer\GAL4^OK386, nej^UAS.cMa

(Bantignies et al., 2000)

Pka-R1^BDK.UAS, Scer\GAL4^RZ4 is a suppressor of wing phenotype of Pka-C1^UAS.Tag:FLAG, Scer\GAL4^RZ4

(Kiger et al., 1999)

NOT Suppressor of

Statement

Reference

Pka-R1^BDK.UAS, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Pdk1^EP837, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in food consumption compared to controls seen in adults expressing sNPF-R^Scer\UAS.cLa under the control of Scer\GAL4^sNPF-R.2.5 is suppressed by co-expression of Pka-R1^BDK.Scer\UAS.

(Hong et al., 2012)

Flies expressing Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^Oamb.4.4 in a Oamb^unspecified heterozygous background show wild type ovulation levels. Wild type ovulation levels are also seen when Scer\GAL80^ts.αTub84B is used to limit expression to 3 days prior to mating.

(Lee et al., 2009)

Expression of Pka-R1^BDK.Scer\UAS, under the control of Scer\GAL4^eve.RN2O, in aCC/RP2 neurons with an Ace^j50 background suppresses the reduction of inward Na⁺ current and the reduction in excitability seen in Ace^j50 alone.

(Baines, 2003)

The lethality of the third instars in the Itp-r83A^1664GAL4/Itp-r83A^90B.0, Scer\GAL4^{Itp-r83A-1664GAL4} genotype may mask an increased developmental delay.

(Venkatesh et al., 2001)

The combination of capt^k01217 and Pka-R1^BDK.Scer\UAS in germ-line clones produces an exaggerated phenotype, with complete fusion of the germline and ectopic actin aggregates at the posterior pole of the syncytium.

(Baum et al., 2000)

Coexpression of Pka-C1^{Scer\UAS.T:Zzzz\FLAG} and Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^RZ4 results in normal wing development. Coexpression of P{UAS-Pka-R1.BDK} and P{UAS-Pka-C1.FLAG} in a Scer\GAL4^e22c background rescues the abdominal denticle belt phenotype of P{UAS-Pka-R1.BDK}, and partially restores thoracic denticle belts.

(Kiger et al., 1999)

The increase in average spontaneous miniature excitatory junctional current amplitude seen in larvae expressing Pka-R1^BDK.Scer\UAS under the control of Scer\GAL4^Mhc.PW is significantly suppressed by dnc^M14.

(Davis et al., 1998)

Reduction or elimination of protein kinase A activity has no effect on the phenotype generated when the G-sα60A pathway is activated in wing epithelial cells; wing duplications that contain blisters are formed when Pka-R1^BDK.Scer\UAS and G-sα60A^{Q215L.Scer\UAS} are co-expressed using Scer\GAL4^30A.

(Wolfgang et al., 1996)

Adult pattern defects are suppressed by a mouse mutant catalytic subunit expressed under the influence of Scer\GAL4.

(Li et al., 1995)

Xenogenetic Interactions

Statement

Reference

Co-expression of Pka-R1^BDK.Scer\UAS suppresses lethality in flies with expression of Hsap\HTT^{128Q.1-208.Scer\UAS} driven by Scer\GAL4^elav-C155 (only tested weak F27B insertion), and suppresses sleep and sleep homeostasis phenotypes (tested F27B) (expression driven by Scer\GAL4^elav.PU).

(Gonzales et al., 2016)

Co-expression of Zzzz\EF^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 suppresses Pka-R1^BDK.Scer\UAS-induced lethality and the associated pupal phenotypes.

(Guichard et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

35550

w^*; P{UAS-Pka-R1.BDK}35

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Pka-R1^BDK.Scer\UAS

Pka-R1^BDK.UAS

Pka-R1^R.UAS

R^dn

(Wolfgang et al., 1996)

UAS-R*

(Apionishev et al., 2005, Ohlmeyer and Kalderon, 1998)

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kalderon

(Baum et al., 2000)

Secondary FlyBase IDs

FBal0042699

References (49)

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