Expression pattern is observed to be the same as the pattern of endogenous nub expression. Expression is not observed in fully delaminated neuroblasts at embryonic stage 9. At embryonic stage 10, expression is also seen in a small subset of ventral nerve cord neuroblasts and ganglion mother cells. Late in embryonic stage 10 and into stage 11, expression is observed in ganglion mother cells and many neuroblasts of the cephalic lobe and the ventral nerve cord. The intensity of neuroblast expression decreases during stage 12.