FlyBase Allele Report: Dmel\CtBP[03463]

General Information

Symbol

Dmel\CtBP⁰³⁴⁶³

Species

D. melanogaster

Name

FlyBase ID

FBal0086957

Feature type

allele

Associated gene

Dmel\CtBP

Associated Insertion(s)

P{PZ}CtBP⁰³⁴⁶³

Carried in Construct

Also Known As

dCtBP⁰³⁴⁶³, P1590, CtBP^P1590

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Zhang and Arnosti, 2011)

Mutagen

P-element activity

(Nibu et al., 1998, Poortinga et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{PZ}CtBP⁰³⁴⁶³

Cytology

Description

The P{PZ} insertion is 7353bp upstream of the putative ATG start codon.

(Fernandez-Funez et al., 2000)

P{PZ} insertion maps within the 5' untranslated intron, intron is located 37bp downstream of the 5' end of the largest CtBP cDNA.

(Nibu et al., 1998)

P{PZ} insertion maps within the CtBP transcription unit.

(Poortinga et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:13,012,243..13,012,243 [+]

(Bi et al., 2022, Park et al., 2022, Zhang and Arnosti, 2011, Basu and Atchison, 2010, Biryukova and Heitzler, 2008, Stathopoulos and Levine, 2005, Bianchi-Frias et al., 2004, Struffi et al., 2004, Fernandez-Funez et al., 2000, Spradling et al., 1999, Nibu et al., 1998, Poortinga et al., 1998, Braun et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^30Q.UAS

(Fernandez-Funez et al., 2000)

modeled by Hsap\ATXN1^82Q.UAS

(Fernandez-Funez et al., 2000)

exacerbates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

abnormal neuroanatomy | pharate adult stage (with Df(3R)ry615)

(Hoang et al., 2010)

abnormal size | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

abnormal size | pharate adult stage (with Df(3R)ry615)

(Hoang et al., 2010)

increased size | adult stage | somatic clone - tissue specific

(Vissers et al., 2020)

lethal | embryonic stage | maternal effect

(Perrimon et al., 1996)

lethal | maternal effect | recessive

(Poortinga et al., 1998)

lethal | pharate adult stage | recessive

(Poortinga et al., 1998)

lethal | recessive

(Spradling et al., 1999, Perrimon et al., 1996)

lethal - all die before end of larval stage

(Hoang et al., 2010)

lethal - all die before end of P-stage (with CtBP^87De-10)

(Hoang et al., 2010, Barolo et al., 2002)

lethal - all die before end of P-stage (with Df(3R)ry615)

(Hoang et al., 2010)

lethal - all die before end of pupal stage | recessive

(Braun et al., 1997)

some die during pharate adult stage (with CtBP^87De-10)

(Barolo et al., 2002)

some die during pupal stage | recessive

(Braun et al., 1997)

visible | adult stage | somatic clone - tissue specific

(Vissers et al., 2020)

visible | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

Phenotype Manifest In

adult cuticle | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

adult head | somatic clone - tissue specific

(Vissers et al., 2020)

anterior dorsocentral bristle | pharate adult stage | increased number

(Stern et al., 2009)

anterior postalar bristle | pharate adult stage | increased number

(Stern et al., 2009)

anterior postalar bristle | pharate adult stage | increased number (with CtBP^87De-10)

(Stern et al., 2009)

anterior scutellar bristle | pharate adult stage (with CtBP^87De-10)

(Stern et al., 2009)

anterior scutellar bristle | pharate adult stage | increased number

(Stern et al., 2009)

anterior scutellar bristle | pharate adult stage | increased number (with CtBP^87De-10)

(Stern et al., 2009)

embryonic/first instar larval cuticle | germline clone | maternal effect | ventral

(Poortinga et al., 1998)

embryonic/first instar larval cuticle | maternal effect | ventral

(Perrimon et al., 1996)

embryonic/larval crystal cell

(Milchanowski et al., 2004)

external sensory organ precursor cell | larval stage | increased number

(Stern et al., 2009)

eye | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

eye | pharate adult stage (with Df(3R)ry615)

(Hoang et al., 2010)

eye | somatic clone - tissue specific

(Vissers et al., 2020)

frontal bristle | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

interocellar bristle | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

interommatidial bristle | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

macrochaeta (with CtBP^87De-10)

(Barolo et al., 2002)

macrochaeta | ectopic | maternal effect

(Poortinga et al., 1998)

macrochaeta | increased number

(Stern et al., 2007)

mesonotum | pharate adult stage

(Stern et al., 2009)

mesonotum | pharate adult stage (with CtBP^87De-10)

(Stern et al., 2009)

ocellar triangle | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

ocellus | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

ommatidium | pharate adult stage | increased number (with CtBP^87De-10)

(Hoang et al., 2010)

ommatidium | pharate adult stage | increased number (with Df(3R)ry615)

(Hoang et al., 2010)

orbital bristle | pharate adult stage | increased number (with CtBP^87De-10)

(Hoang et al., 2010)

posterior dorsocentral bristle | pharate adult stage (with CtBP^87De-10)

(Stern et al., 2009)

posterior dorsocentral bristle | pharate adult stage | increased number

(Stern et al., 2009)

posterior dorsocentral bristle | pharate adult stage | increased number (with CtBP^87De-10)

(Stern et al., 2009)

posterior postalar bristle | pharate adult stage | increased number

(Stern et al., 2009)

posterior postalar bristle | pharate adult stage | increased number (with CtBP^87De-10)

(Stern et al., 2009)

posterior scutellar bristle | pharate adult stage (with CtBP^87De-10)

(Stern et al., 2009)

posterior scutellar bristle | pharate adult stage | increased number

(Stern et al., 2009)

posterior scutellar bristle | pharate adult stage | increased number (with CtBP^87De-10)

(Stern et al., 2009)

posterior supraalar bristle | pharate adult stage | increased number

(Stern et al., 2009)

posterior supraalar bristle | pharate adult stage | increased number (with CtBP^87De-10)

(Stern et al., 2009)

scutellum & macrochaeta | maternal effect

(Poortinga et al., 1998)

scutum & macrochaeta | maternal effect

(Poortinga et al., 1998)

segment | maternal effect

(Perrimon et al., 1996)

ventral denticle belt | maternal effect

(Poortinga et al., 1998)

vertical bristle 2 | pharate adult stage (with CtBP^87De-10)

(Hoang et al., 2010)

vertical bristle | pharate adult stage | increased number (with CtBP^87De-10)

(Hoang et al., 2010)

Detailed Description

Statement

Reference

Heterozygous CtBP⁰³⁴⁶³ mutant flies carrying two copies of CtBP^{NAD.T:Zzzz\FLAG} show notched wings, while no phenotype is observed when these transgenes are present in a wild-type background or if only one copy of CtBP^{NAD.T:Zzzz\FLAG} is present.

(Zhang and Arnosti, 2011)

CtBP^87De-10/CtBP⁰³⁴⁶³ pharate adults exhibit head bristle defects: occasional duplicated vertical and orbital bristles; occasional double and/or misoriented orbital and postvertical bristles; and variable loss of interocellar and frontal bristles when compared to controls. Also in those transheterozygotes, the ocelli and surrounding ocellar cuticle are somewhat larger while the distance across the dorsal head between eyes are similar compared to controls.

CtBP⁰³⁴⁶³/CtBP^87De-10 pharate adults exhibit larger and mildly rough eyes with a decrease in the number of interommatidial bristles, and (those animals and also CtBP⁰³⁴⁶³/Df(3R)ry615 animals) exhibit a significant increase in the number of ommatidia when compared to controls.

(Hoang et al., 2010)

CtBP⁰³⁴⁶³/CtBP^87De-10 heterozygous and CtBP⁰³⁴⁶³ homozygous pharates display extra dorsal thoracic mechanosensory bristles (additional bristles attached to sockets) and, less frequently, double bristles (two bristles housed in the same socket structure) and bald cuticle (complete loss of both bristles and their associated sockets). Only a subset of bristles are affected.

CtBP⁰³⁴⁶³ homozygous wing discs contain two, instead of the normal one, sens-positive posterior dorsocentral SOP cells.

(Stern et al., 2009)

Single cell class IV dendrite arborisation (da) neuron clones that are homozygous for CtBP⁰³⁴⁶³ do not show defects in the establishment or maintenance of dendritic tiling.

(Parrish et al., 2007)

2.7% of CtBP⁰³⁴⁶³ heterozygotes show an extra macrochaete phenotype on the dorsal thorax.

(Stern et al., 2007)

The average number of crystal cells per embryo is reduced (to 13.3) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average).

(Milchanowski et al., 2004)

CtBP⁰³⁴⁶³/CtBP^87De-10 pharate adults show bristle shaft defects and shaft loss.

(Barolo et al., 2002)

When dissected from the pupal case homozygotes exhibit duplicated and ectopic bristles on the notum and scutellum. Loss of maternal CtBP does not result in a neurogenic phenotype. Larval cuticles generated from germline mosaics exhibit a severely disrupted pattern: segmentation defects resulting in pair-wise fusions and large holes in the ventral cuticle.

(Poortinga et al., 1998)

Germline clones produce eggs with patterning defects: embryos exhibit ventral holes and segment fusion.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

CtBP⁰³⁴⁶³ has increased size | adult stage | somatic clone - tissue specific phenotype, enhanceable by sav²/sav²

(Vissers et al., 2020)

CtBP⁰³⁴⁶³ has visible | adult stage | somatic clone - tissue specific phenotype, enhanceable by sav²/sav²

(Vissers et al., 2020)

Enhancer of

Statement

Reference

CtBP[+]/CtBP⁰³⁴⁶³ is an enhancer of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

CtBP⁰³⁴⁶³ is an enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

NOT Enhancer of

Statement

Reference

CtBP[+]/CtBP⁰³⁴⁶³ is a non-enhancer of visible phenotype of Df(1)os¹

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of homeotic | dominant | male | adult stage phenotype of Pc¹

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of visible | dominant | male | adult stage phenotype of Pc¹

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of homeotic | dominant | male | adult stage phenotype of Pc³

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of visible | dominant | male | adult stage phenotype of Pc³

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor of visible | adult stage phenotype of Scer\GAL4^sca-109-68, ham^UAS.cMa

(Endo et al., 2011)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor of partially lethal phenotype of Scer\GAL4^nanos.PG, run^UAS.cLa

(Wheeler et al., 2002)

NOT Suppressor of

Statement

Reference

CtBP[+]/CtBP⁰³⁴⁶³ is a non-suppressor of visible phenotype of Df(1)os¹

(Mukherjee et al., 2006)

Other

Statement

Reference

CtBP⁰³⁴⁶³, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa has visible | adult stage phenotype

(Worley et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

CtBP⁰³⁴⁶³ has eye | somatic clone - tissue specific phenotype, enhanceable by sav²/sav²

(Vissers et al., 2020)

CtBP⁰³⁴⁶³ has adult head | somatic clone - tissue specific phenotype, enhanceable by sav²/sav²

(Vissers et al., 2020)

Enhancer of

Statement

Reference

CtBP[+]/CtBP⁰³⁴⁶³ is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

CtBP[+]/CtBP⁰³⁴⁶³ is an enhancer of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

CtBP⁰³⁴⁶³ is an enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

CtBP⁰³⁴⁶³ is an enhancer of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

CtBP⁰³⁴⁶³ is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

NOT Enhancer of

Statement

Reference

CtBP[+]/CtBP⁰³⁴⁶³ is a non-enhancer of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

CtBP[+]/CtBP⁰³⁴⁶³ is a non-enhancer of eye phenotype of Df(1)os¹

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor of metathoracic leg | male phenotype of Pc¹

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of prothoracic leg | ectopic | male phenotype of Pc¹

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of mesothoracic leg | male phenotype of Pc¹

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of metathoracic leg | male phenotype of Pc³

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of mesothoracic leg | male phenotype of Pc³

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of prothoracic leg | ectopic | male phenotype of Pc³

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of mesothoracic leg | male phenotype of Scm^D1

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | partially of prothoracic leg | ectopic | male phenotype of Scm^D1

(Bi et al., 2022)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor of notal microchaeta | adult stage phenotype of Scer\GAL4^sca-109-68, ham^UAS.cMa

(Endo et al., 2011)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor of tormogen cell | adult stage phenotype of Scer\GAL4^sca-109-68, ham^UAS.cMa

(Endo et al., 2011)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor of trichogen cell | ectopic | adult stage phenotype of Scer\GAL4^sca-109-68, ham^UAS.cMa

(Endo et al., 2011)

CtBP[+]/CtBP⁰³⁴⁶³ is a suppressor | maternal effect of embryonic/first instar larval cuticle | pair rule expression pattern phenotype of gro^E47/gro[+], hry³⁰/hry²⁶

(Phippen et al., 2000)

CtBP⁰³⁴⁶³ is a suppressor of phenotype of hry²⁶

(Poortinga et al., 1998)

CtBP⁰³⁴⁶³ is a suppressor of phenotype of hry³⁰

(Poortinga et al., 1998)

NOT Suppressor of

Statement

Reference

CtBP[+]/CtBP⁰³⁴⁶³ is a non-suppressor of eye phenotype of Df(1)os¹

(Mukherjee et al., 2006)

Other

Statement

Reference

CtBP⁰³⁴⁶³, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa has wing | ectopic phenotype

(Worley et al., 2018)

CtBP⁰³⁴⁶³, Polr2B^wimp has ventral denticle belt phenotype

(Poortinga et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of egr^UAS.cUa under the control of Scer\GAL4^rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in CtBP⁰³⁴⁶³/+ background leads to ectopic wings compared to controls.

(Worley et al., 2018)

Sir2^05327a does not show a dominant synthetic lethal interaction with CtBP⁰³⁴⁶³.

(Rosenberg and Parkhurst, 2002)

The partial lethality due to run^Scer\UAS.cLa; Scer\GAL4^nos.PG (3% viable) is partially suppressed by maternal heterozygosity for CtBP⁰³⁴⁶³ (rescues to 15% viable).

(Wheeler et al., 2002)

The pair-rule phenotype of h²⁶/h³⁰ embryos is suppressed by one maternal copy of CtBP⁰³⁴⁶³. The enhancement of the pair-rule phenotype of h²⁶/h³⁰ embryos caused by one maternal copy of gro^E47 is reversed when one copy of CtBP⁰³⁴⁶³ is also present maternally.

(Phippen et al., 2000)

Embryos derived from mothers transheterozygous for RpII140^wimp and CtBP⁰³⁴⁶³ die and cuticle preparations of the embryos show segmentation defects ranging from pair-wise fusion of adjacent denticle belts to more widespread denticle fusions.

(Poortinga et al., 1998)

Xenogenetic Interactions

Statement

Reference

One copy of CtBP⁰³⁴⁶³ does not enhance the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

One copy of CtBP⁰³⁴⁶³ enhances the retinal phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

Complementation and Rescue Data

Fails to complement

CtBP^87De-10

(Poortinga et al., 1998)

CtBP^87De-1

(Poortinga et al., 1998)

Partially rescued by

CtBP⁰³⁴⁶³/CtBP^87De-10 is partially rescued by CtBP^Tag:FLAG

(Zhang and Arnosti, 2011)

CtBP⁰³⁴⁶³ is partially rescued by CtBP^Tag:FLAG

(Zhang and Arnosti, 2011)

CtBP⁰³⁴⁶³ is partially rescued by CtBP^S.Tag:FLAG

(Zhang and Arnosti, 2011)

CtBP⁰³⁴⁶³ is partially rescued by CtBP^L.Tag:FLAG

(Zhang and Arnosti, 2011)

CtBP⁰³⁴⁶³ is partially rescued by CtBP^CAT.Tag:FLAG

(Zhang and Arnosti, 2011)

Not rescued by

CtBP⁰³⁴⁶³ is not rescued by CtBP^NAD.Tag:FLAG

(Zhang and Arnosti, 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

A. Spradling.

Comments

Mobilisation of the P{PZ} element demonstrates the insertion is responsible for the mutant phenotype.

(Poortinga et al., 1998)

Complements: l(3)j2C3^j2C3. Complements: l(3)neo39¹. Complements: l(3)neo40¹.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

03463

(Mukherjee et al., 2006)

CtBP⁰³⁴⁶³

(Bi et al., 2022, Park et al., 2022, Vissers et al., 2020, Johns et al., 2018, Worley et al., 2018, Endo et al., 2011, Walrad et al., 2011, Zhang and Arnosti, 2011, Basu and Atchison, 2010, Hoang et al., 2010, Parrish et al., 2007, Cowden and Levine, 2003)

CtBP^32P

(Bi et al., 2022)

CtBP^P1590

(Bi et al., 2022, Domanitskaya and Schüpbach, 2012, Technau et al., 2012, Cinnamon et al., 2004)

CtBP^mat-

(Cinnamon et al., 2004)

P11590

(Milchanowski et al., 2004)

P1590

(Nibu et al., 2003, Zhao et al., 2002, Phippen et al., 2000, Poortinga et al., 1998)

ctbp⁰³⁴⁶³

(Srinivasan and Atchison, 2004)

dCtBP⁰³⁴⁶³

(Stern et al., 2009, Branco et al., 2008, Stern et al., 2007, Barolo et al., 2002, Rosenberg and Parkhurst, 2002, Wheeler et al., 2002, Wen et al., 2000)

l(3)03463

(Spradling et al., 1999)

l(3)03463⁰³⁴⁶³

Name Synonyms

Secondary FlyBase IDs

References (44)

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