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General Information
Symbol
Dmel\Dadj1E4
Species
D. melanogaster
Name
FlyBase ID
FBal0086978
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dad-lacZ, Dadj1E4-lacZ, J1E4, Dad1jE4
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{lacW}Dadj1E4
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The neuromuscular junctions of Dadj1E4 homozygous transheterozygous third instar larvae present significantly more boutons than controls.

Dadj1E4 mutant third instar larvae show increased number of boutons on neuromuscular junctions, whereas the bouton size is comparable to controls.

Dadj1E4/+ larvae display a slight increase in the fraction of small boutons on the neuromuscular junctions in third instar larvae compared to controls.

Homozygous third instar larvae show an increase in bouton number and satellite bouton number at the neuromuscular junction compared to controls.

The neuromuscular junctions of homozygous larvae show reduced uptake of FM1-43 dye compared to wild type, indicating a defect in endocytosis.

The average number of boutons at the neuromuscular junction is increased in mutant larvae compared to controls.

Homozygous third instar larvae show a significant increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction compared to wild type.

Homozygous and Dadj1E4/Dad271-68 larvae show an increase in both the total number of boutons and the number of satellite boutons at the neuromuscular junction compared to wild type.

Homozygous flies have no discernible mutant wing phenotype.

Homozygotes/hemizygotes have ectopic vein material in the wing.

Heterozygotes show a direct effect on the shape of the wing.

In eggs laid by Dadj1E4/+ females, 3% exhibit short dorsal appendages and ectopic material deposited at the base.

Homozygous and Dad271-68/Dadj1E4 larvae show no significant change in the number of synaptic boutons at the neuromuscular junction.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

Dadj1E4/Dad[+] is an enhancer of visible phenotype of DgdsRNA.UAS, Scer\GAL4Tub.PU

Dadj1E4 is an enhancer of visible phenotype of nmoadk1

Dadj1E4/Dad[+] is an enhancer of visible | heat sensitive phenotype of HC2.hs

Suppressor of
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

Dadj1E4/Dad[+] is an enhancer of neuromuscular junction phenotype of Acsl05847/Acsl8

Dadj1E4/Dad[+] is an enhancer of NMJ bouton | increased number phenotype of Acsl05847/Acsl8

Dadj1E4 is an enhancer of wing vein | ectopic phenotype of nmoadk1

Dadj1E4/Dad[+] is an enhancer of wing vein | ectopic | heat sensitive phenotype of HC2.hs

Dadj1E4/Dad[+] is an enhancer of wing | heat sensitive phenotype of HC2.hs

Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The synaptic overgrowth phenotype (increased number of boutons at neuromuscular junctions) characteristic for third instar larvae homozygous for Dadj1E4 is completely suppressed by Nlg4KO10 homozygosity and the resulting number of boutons per NMJ is comparable to Nlg4KO10 single mutants and significantly lower than in wild-type controls.

The migration of wing disc cells expressing GliScer\UAS.cSa under the control of Scer\GAL4ap.PU into the non-expressing side of the disc is completely blocked by one copy of Dadj1E4.

dpr11MI02231/Dadj1E4 or DIP-γMI03222/Dadj1E4 transheterozygote third instar larvae have extra satellite boutons at the neuromuscular junction (NMJ) compared to wild type and heterozygotes.

cupΔ212/+;Dadj1E4/+ transheterozygotes show significant increases in satellite boutons at the NMJ of third instar larvae compared to either single heterozygote.

S6KL140/+ ; Dadj1E4/+ transheterozygous mutants exhibit an increase in total bouton number not seen in either heterozygote alone.

Dadj1E4/+ significantly increases synaptic overgrowth of Acsl05847/Acsl8 mutants.

Fmr1Δ50M Dadj1E4 double heterozygous third instar larvae show an increase in bouton number and satellite bouton number at the neuromuscular junction compared to controls.

brat2L-192-9/+ ; Dadj1E4/+ double heterozygotes show a significant increase in the number of satellite boutons at the larval neuromuscular junction compared to wild type.

The increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in homozygous Dadj1E4 third instar larvae is not altered if they are also mutant for RhoGAP92B1/RhoGAP92B2.

The decrease in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in third instar RhoGAP92B1/RhoGAP92B2 larvae is significantly suppressed if the larvae are also mutant for Dadj1E4/+.

One copy of Dadj1E4 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.

One copy of Dadj1E4 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU but produces extra wing vein material.

One copy of Dadj1E4 in a DysE6/+ background results in posterior crossvein defects.

One copy of Dadj1E4 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

Expression of either Dap160Scer\UAS.cKa or nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155 suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in homozygous Dadj1E4 larvae.

The severity of the ectopic wing vein phenotype seen in nmoadk1 flies is increased in Dadj1E4 ; nmoadk1 double mutants.

Xenogenetic Interactions
Statement
Reference

The neuromuscular junction growth defects (reduced number of NMJ boutons, decreased NMJ area, length of the longest NMJ branch) as well as larval crawling defects characteristic for third instar larvae expressing Hsap\TARDBPScer\UAS.cHa under the control of Scer\GAL4VGlut.PD can be partially rescued by combination with Dadj1E4 in homozygous state. None of the NMJ growth aberrations can be restored by combination with just single copy of Dadj1E4, except the highly increased fraction of small boutons on the main NMJ axis (which is slightly increased also in Dadj1E4/+ larvae alone).

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (14)
References (31)