FlyBase Allele Report: Dmel\Dad[j1E4]

(Toraason et al., 2024, Park et al., 2022, Ogiso et al., 2011, Dworkin and Gibson, 2006, Muzzopappa and Wappner, 2005, Ryder and Roote, 2004.11.10, Henderson et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.cHa

(Deshpande et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The neuromuscular junctions of Dad^j1E4 homozygous transheterozygous third instar larvae present significantly more boutons than controls.

(Connolly et al., 2019)

Dad^j1E4 mutant third instar larvae show increased number of boutons on neuromuscular junctions, whereas the bouton size is comparable to controls.

(Zhang et al., 2017)

Dad^j1E4/+ larvae display a slight increase in the fraction of small boutons on the neuromuscular junctions in third instar larvae compared to controls.

(Deshpande et al., 2016)

Homozygous third instar larvae show an increase in bouton number and satellite bouton number at the neuromuscular junction compared to controls.

(Nahm et al., 2013)

The neuromuscular junctions of homozygous larvae show reduced uptake of FM1-43 dye compared to wild type, indicating a defect in endocytosis.

(Shi et al., 2013)

The average number of boutons at the neuromuscular junction is increased in mutant larvae compared to controls.

(Rodal et al., 2011)

Homozygous third instar larvae show a significant increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction compared to wild type.

(Nahm et al., 2010)

Homozygous and Dad^j1E4/Dad^271-68 larvae show an increase in both the total number of boutons and the number of satellite boutons at the neuromuscular junction compared to wild type.

(O'Connor-Giles et al., 2008)

Homozygous flies have no discernible mutant wing phenotype.

(Zeng et al., 2007)

Homozygotes/hemizygotes have ectopic vein material in the wing.

Heterozygotes show a direct effect on the shape of the wing.

(Dworkin and Gibson, 2006)

In eggs laid by Dad^j1E4/+ females, 3% exhibit short dorsal appendages and ectopic material deposited at the base.

(Muzzopappa and Wappner, 2005)

Homozygous and Dad^271-68/Dad^j1E4 larvae show no significant change in the number of synaptic boutons at the neuromuscular junction.

(McCabe et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dad^j1E4 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Nlg4^KO10/Nlg4^KO10

(Zhang et al., 2017)

Dad^j1E4 has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^elav-C155/nwk^UAS.cCa

(O'Connor-Giles et al., 2008)

Dad^j1E4 has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Dap160^UAS.cKa

(O'Connor-Giles et al., 2008)

NOT suppressed by

Statement

Reference

Dad^j1E4 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Enhancer of

Statement

Reference

Dad^j1E4 is an enhancer of visible | adult stage phenotype of shn^zf1.2.4.5

(Toraason et al., 2024)

Dad^j1E4/Dad[+] is an enhancer of abnormal neuroanatomy | third instar larval stage | semidominant phenotype of cup^Δ212

(Menon et al., 2015)

Dad^j1E4/Dad[+] is an enhancer of abnormal neuroanatomy | larval stage phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸

(Liu et al., 2014)

Dad^j1E4/Dad[+] is an enhancer of visible phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Dad^j1E4 is an enhancer of visible phenotype of nmo^adk1

(Zeng et al., 2007)

Dad^j1E4/Dad[+] is an enhancer of visible | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

Suppressor of

Statement

Reference

Dad^j1E4/Dad^j1E4 is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Dad^j1E4/Dad[+] is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Dad^j1E4/Dad[+] is a suppressor of increased cell death phenotype of Gli^UAS.cSa, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Dad^j1E4/Dad[+] is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Dad^j1E4/Dad[+] is a suppressor of visible phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

Dad^j1E4/Dad[+] is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Other

Statement

Reference

DIP-γ^MI03222, Dad^j1E4/Dad[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Carrillo et al., 2015)

Dad^j1E4/Dad[+], dpr11^MI02231 has abnormal neuroanatomy | third instar larval stage phenotype

(Carrillo et al., 2015)

Dad^j1E4/Dad[+], Fmr1^Δ50M has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Nahm et al., 2013)

Dad^j1E4/Dad[+], brat^2L-192-9 has abnormal neuroanatomy | third instar larval stage phenotype

(Shi et al., 2013)

Dad^j1E4/Dad[+], Dys^E6 has visible phenotype

(Kucherenko et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dad^j1E4 has muscle cell of A1-7 lateral longitudinal muscle 1 | third instar larval stage phenotype, suppressible | partially by Cln7^84D/Cln7^84D

(Connolly et al., 2019)

Dad^j1E4 has NMJ bouton | increased number | third instar larval stage phenotype, suppressible | partially by Cln7^84D/Cln7^84D

(Connolly et al., 2019)

Dad^j1E4 has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Nlg4^KO10/Nlg4^KO10

(Zhang et al., 2017)

Dad^j1E4 has NMJ bouton | increased number | larval stage phenotype, suppressible by Scer\GAL4^elav-C155/nwk^UAS.cCa

(O'Connor-Giles et al., 2008)

Dad^j1E4 has NMJ bouton | increased number | larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Dap160^UAS.cKa

(O'Connor-Giles et al., 2008)

NOT suppressed by

Statement

Reference

Dad^j1E4 has NMJ bouton | third instar larval stage phenotype, non-suppressible by RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Enhancer of

Statement

Reference

Dad^j1E4 is an enhancer of wing vein | ectopic phenotype of shn^zf1.2.4.5

(Toraason et al., 2024)

Dad^j1E4/Dad[+] is an enhancer of NMJ bouton | third instar larval stage | increased number phenotype of cup^Δ212

(Menon et al., 2015)

Dad^j1E4/Dad[+] is an enhancer of neuromuscular junction phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸

(Liu et al., 2014)

Dad^j1E4/Dad[+] is an enhancer of NMJ bouton | increased number phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸

(Liu et al., 2014)

Dad^j1E4/Dad[+] is an enhancer of posterior crossvein phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Dad^j1E4 is an enhancer of wing vein | ectopic phenotype of nmo^adk1

(Zeng et al., 2007)

Dad^j1E4/Dad[+] is an enhancer of wing vein | ectopic | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

Dad^j1E4/Dad[+] is an enhancer of wing | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

Suppressor of

Statement

Reference

Dad^j1E4/Dad^j1E4 is a suppressor of muscle cell of A1-7 lateral longitudinal muscle 1 | third instar larval stage phenotype of Cln7^84D

(Connolly et al., 2019)

Dad^j1E4/Dad^j1E4 is a suppressor of NMJ bouton | third instar larval stage phenotype of Cln7^84D

(Connolly et al., 2019)

Dad^j1E4/Dad^j1E4 is a suppressor | partially of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Dad^j1E4/Dad^j1E4 is a suppressor | partially of NMJ bouton | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Dad^j1E4/Dad[+] is a suppressor | partially of NMJ bouton | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Dad^j1E4/Dad[+] is a suppressor of wing disc phenotype of Gli^UAS.cSa, Scer\GAL4^ap.PU

(Sharifkhodaei et al., 2016)

Dad^j1E4/Dad[+] is a suppressor of NMJ bouton | third instar larval stage phenotype of RhoGAP92B¹/RhoGAP92B²

(Nahm et al., 2010)

Dad^j1E4/Dad[+] is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Dad^j1E4/Dad[+] is a suppressor of posterior crossvein phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

NOT Suppressor of

Statement

Reference

Dad^j1E4/Dad[+] is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^VGlut1.PD

(Deshpande et al., 2016)

Other

Statement

Reference

Dad^j1E4/Dad[+], dpr11^MI02231 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Carrillo et al., 2015)

Dad^j1E4/Dad[+], dpr11^MI02231 has NMJ bouton | increased number | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222, Dad^j1E4/Dad[+] has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Carrillo et al., 2015)

DIP-γ^MI03222, Dad^j1E4/Dad[+] has NMJ bouton | increased number | third instar larval stage phenotype

(Carrillo et al., 2015)

Dad^j1E4/Dad[+], S6KL¹⁴⁰ has NMJ bouton | increased number phenotype

(Zhao et al., 2015)

Dad^j1E4/Dad[+], Fmr1^Δ50M has NMJ bouton | increased number | third instar larval stage phenotype

(Nahm et al., 2013)

Dad^j1E4/Dad[+], brat^2L-192-9 has NMJ bouton | increased number | third instar larval stage phenotype

(Shi et al., 2013)

Dad^j1E4/Dad[+], Dys^E6 has posterior crossvein phenotype

(Kucherenko et al., 2008)

Dad^j1E4/Dad[+], Dys^RNAi.NH2.UAS has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Dad^j1E4/Dad[+], Dys^RNAi.C.UAS has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The synaptic overgrowth phenotype (increased number of boutons at neuromuscular junctions) characteristic for third instar larvae homozygous for Dad^j1E4 is completely suppressed by Nlg4^KO10 homozygosity and the resulting number of boutons per NMJ is comparable to Nlg4^KO10 single mutants and significantly lower than in wild-type controls.

(Zhang et al., 2017)

The migration of wing disc cells expressing Gli^Scer\UAS.cSa under the control of Scer\GAL4^ap.PU into the non-expressing side of the disc is completely blocked by one copy of Dad^j1E4.

(Sharifkhodaei et al., 2016)

dpr11^MI02231/Dad^j1E4 or DIP-γ^MI03222/Dad^j1E4 transheterozygote third instar larvae have extra satellite boutons at the neuromuscular junction (NMJ) compared to wild type and heterozygotes.

(Carrillo et al., 2015)

cup^Δ212/+;Dad^j1E4/+ transheterozygotes show significant increases in satellite boutons at the NMJ of third instar larvae compared to either single heterozygote.

(Menon et al., 2015)

S6KL¹⁴⁰/+ ; Dad^j1E4/+ transheterozygous mutants exhibit an increase in total bouton number not seen in either heterozygote alone.

(Zhao et al., 2015)

Dad^j1E4/+ significantly increases synaptic overgrowth of Acsl⁰⁵⁸⁴⁷/Acsl⁸ mutants.

(Liu et al., 2014)

Fmr1^Δ50M Dad^j1E4 double heterozygous third instar larvae show an increase in bouton number and satellite bouton number at the neuromuscular junction compared to controls.

(Nahm et al., 2013)

brat^2L-192-9/+ ; Dad^j1E4/+ double heterozygotes show a significant increase in the number of satellite boutons at the larval neuromuscular junction compared to wild type.

(Shi et al., 2013)

The increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in homozygous Dad^j1E4 third instar larvae is not altered if they are also mutant for RhoGAP92B¹/RhoGAP92B².

The decrease in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in third instar RhoGAP92B¹/RhoGAP92B² larvae is significantly suppressed if the larvae are also mutant for Dad^j1E4/+.

(Nahm et al., 2010)

One copy of Dad^j1E4 strongly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU but produces extra wing vein material.

One copy of Dad^j1E4 strongly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

One copy of Dad^j1E4 in a Dys^E6/+ background results in posterior crossvein defects.

One copy of Dad^j1E4 enhances the posterior crossvein phenotype seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2008)

Expression of either Dap160^Scer\UAS.cKa or nwk^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in homozygous Dad^j1E4 larvae.

(O'Connor-Giles et al., 2008)

The severity of the ectopic wing vein phenotype seen in nmo^adk1 flies is increased in Dad^j1E4 ; nmo^adk1 double mutants.

(Zeng et al., 2007)

Xenogenetic Interactions

Statement

Reference

The neuromuscular junction growth defects (reduced number of NMJ boutons, decreased NMJ area, length of the longest NMJ branch) as well as larval crawling defects characteristic for third instar larvae expressing Hsap\TARDBP^Scer\UAS.cHa under the control of Scer\GAL4^VGlut.PD can be partially rescued by combination with Dad^j1E4 in homozygous state. None of the NMJ growth aberrations can be restored by combination with just single copy of Dad^j1E4, except the highly increased fraction of small boutons on the main NMJ axis (which is slightly increased also in Dad^j1E4/+ larvae alone).

(Deshpande et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10305

y¹ w¹¹¹⁸; P{lacW}Dad^j1E4/TM3, Sb¹

Kyoto

111046

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{lacW}Dad^j1E4/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

Dad^1E4

(Henderson et al., 1999)

Dad^1jE4

(Carrillo et al., 2015)

Dad^J1e4

(O'Connor-Giles et al., 2008, McCabe et al., 2004)

Dad^j1E4

(Li et al., 2020, Sharifkhodaei et al., 2016, Carrillo et al., 2015, Ogiso et al., 2011, Kucherenko et al., 2008, Zeng et al., 2007)

Dad^j1e4

(Connolly et al., 2019, Ryder and Roote, 2004.11.10)

J1E4

(Dworkin et al., 2011, Dworkin and Gibson, 2006)

dad^1jE4

(Menon et al., 2015)

dad^J184

(Zhao et al., 2015)

dad^J1E4

(Deshpande et al., 2016, Shi et al., 2013, Dworkin and Gibson., 2006)

dad^J1e4

(Liu et al., 2014, Nahm et al., 2013, Nahm et al., 2010)

dad^j1E4

(Toraason et al., 2024, Park et al., 2022, Vuilleumier et al., 2019, Huang et al., 2016, Rodal et al., 2011, Muzzopappa and Wappner, 2005)

dadj1E4

(Zhang et al., 2017)

l(3)j1E4

(Spradling et al., 1999)

l(3)j1E4^j1E4

(Johannes and Preiss, 2002)

Name Synonyms

Secondary FlyBase IDs

References (33)

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