The synaptic overgrowth phenotype (increased number of boutons at neuromuscular junctions) characteristic for third instar larvae homozygous for Dadj1E4 is completely suppressed by Nlg4KO10 homozygosity and the resulting number of boutons per NMJ is comparable to Nlg4KO10 single mutants and significantly lower than in wild-type controls.
The increase in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in homozygous Dadj1E4 third instar larvae is not altered if they are also mutant for RhoGAP92B1/RhoGAP92B2.
The decrease in bouton number/muscle area and satellite bouton number at the neuromuscular junction which is seen in third instar RhoGAP92B1/RhoGAP92B2 larvae is significantly suppressed if the larvae are also mutant for Dadj1E4/+.
One copy of Dadj1E4 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU but produces extra wing vein material.
One copy of Dadj1E4 strongly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU but produces extra wing vein material.
Expression of either Dap160Scer\UAS.cKa or nwkScer\UAS.cCa under the control of Scer\GAL4elav-C155 suppresses the increase in the total number of boutons and in the number of satellite boutons at the neuromuscular junction that is seen in homozygous Dadj1E4 larvae.
The neuromuscular junction growth defects (reduced number of NMJ boutons, decreased NMJ area, length of the longest NMJ branch) as well as larval crawling defects characteristic for third instar larvae expressing Hsap\TARDBPScer\UAS.cHa under the control of Scer\GAL4VGlut.PD can be partially rescued by combination with Dadj1E4 in homozygous state. None of the NMJ growth aberrations can be restored by combination with just single copy of Dadj1E4, except the highly increased fraction of small boutons on the main NMJ axis (which is slightly increased also in Dadj1E4/+ larvae alone).