FlyBase Allele Report: Dmel\CSN5[L4032]

(Gibert et al., 2005, Muzzopappa and Wappner, 2005, Doronkin et al., 2002, Suh et al., 2002, Beaton, 2000.1.31, Freilich et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage (with CSN5^ex21)

(Doronkin et al., 2002)

dorsal appendage | germline clone

(Doronkin et al., 2002)

egg | germline clone

(Doronkin et al., 2002)

egg chorion | germline clone

(Doronkin et al., 2002)

fusome | germline clone | oogenesis

(Doronkin et al., 2003)

germarium

(Doronkin et al., 2003)

larva

(Freilich et al., 1999)

nurse cell (with CSN5^ex13)

(Doronkin et al., 2003)

nurse cell (with CSN5^ex21)

(Doronkin et al., 2003)

nurse cell spectrosome | germline clone

(Doronkin et al., 2003)

primordial germ cell | germline clone

(Doronkin et al., 2002)

pupa

(Freilich et al., 1999)

Detailed Description

Statement

Reference

6.3% of egg chambers in CSN5^ex21/CSN5^L4032 flies raised at 18^oC have twice the normal number of germ cells (n>500). Oogenesis is normal in CSN5^L4032/+ flies. 10.1% of egg chambers in CSN5^ex13/CSN5^L4032 flies raised at 18^oC have twice the normal number of germ cells (n>500). In germaria carrying CSN5^L4032 germ-line clones, the fusome is often less branched, and sometimes there were more individual fusomes than in wild-type germaria. These germaria contain unbranched fusomes and spherical spectrosomes (fusome precursors) not only at the anterior, but also more posteriorly. Both M-phase and S phase markers can be seen much more posteriorly in these germaria than in wild-type.

(Doronkin et al., 2003)

Females carrying homozygous germline clones lay eggs with a range of abnormal phenotypes that are affected by temperature. Flies raised at 25^oC lay eggs with phenotypes closest to normal. At 18^oC, many of the defective eggs have fused dorsal appendages. At 29^oC, there is an increasing frequency of properly separated, but short, dorsal appendages and dorsalised eggs. Some eggs have an unusually weak eggshell. Somatic clones induced in the ovary do not result in eggshell defects. At 29^oC, homozygotes die during early development and less than 1% of the mutant larvae become prepupae. At 18^oC. 90% of mutant larvae pupariate and 1-2% escape as adults. The number of pole cells is often reduced in embryos derived from homozygous female germline clones and in some cases the pole cells are not tightly clustered but are found in a loose, non-contiguous group near, but not tightly associated with the posterior end of the embryo. 5-15% of eggs laid by CSN5^ex21/CSN5^L4032 females have fused dorsal appendages.

(Doronkin et al., 2002)

A few embryos are recovered from females containing homozygous germ-line clones. These embryos cannot undergo the cell movements required for gastrulation.

(Oron et al., 2002)

Homozygous mutant embryos hatch normally and proceed through first and second larval instars without any apparent defect. Developmental retardation was apparent during the middle of the third larval instar. 10-30% of larvae become increasingly sluggish and flaccid and do not reach pupariation. The majority of larvae pupate but cease to develop, less than 10% reach pharate adult stage. No adults eclose. No cuticular abnormalities are apparent these adults.

(Freilich et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

CSN5^L4032/CSN5^ex21 has increased cell number | oogenesis phenotype, suppressible by CycE^AR95/CycE[+]

(Doronkin et al., 2003)

CSN5^L4032/CSN5^ex21 has increased cell number | oogenesis phenotype, suppressible by CycE[+]/CycE⁰⁵²⁰⁶

(Doronkin et al., 2003)

Other

Statement

Reference

CSN5^L4032, Rpn6^k00103 has increased occurrence of cell division | oogenesis phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032 has increased cell death | oogenesis phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032 has increased occurrence of cell division | oogenesis phenotype

(Doronkin et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

CSN5^L4032 has wing phenotype, enhanceable by trn^28.4/trn[+]

(Doronkin et al., 2003)

Suppressed by

Statement

Reference

CSN5^L4032/CSN5^ex21 has nurse cell phenotype, suppressible by CycE^AR95/CycE[+]

(Doronkin et al., 2003)

CSN5^L4032/CSN5^ex21 has nurse cell phenotype, suppressible | partially by CycE[+]/CycE⁰⁵²⁰⁶

(Doronkin et al., 2003)

CSN5^L4032/CSN5^ex13 has nurse cell phenotype, suppressible | partially by CycE[+]/CycE⁰⁵²⁰⁶

(Doronkin et al., 2003)

CSN5^L4032/CSN5^ex21 has dorsal appendage phenotype, suppressible by mei-41^unspecified/mei-41^unspecified

(Doronkin et al., 2002)

Enhancer of

Statement

Reference

CSN5^L4032/CSN5[+] is an enhancer of female germline stem cell | germline clone phenotype of slmb²

(Muzzopappa and Wappner, 2005)

CSN5^L4032/CSN5[+] is an enhancer of dorsal appendage phenotype of grk^HF

(Doronkin et al., 2002)

CSN5^L4032/CSN5[+] is an enhancer of dorsal appendage phenotype of Egfr^f2

(Doronkin et al., 2002)

CSN5^L4032/CSN5[+] is an enhancer of dorsal appendage phenotype of grk^2B6

(Doronkin et al., 2002)

Other

Statement

Reference

CSN5^L4032, slmb² has germarium | germline clone phenotype

(Muzzopappa and Wappner, 2005)

CSN5^L4032/CSN5[+], slmb² has germarium | germline clone phenotype

(Muzzopappa and Wappner, 2005)

CSN4^k08018, CSN5^L4032 has ovary phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032 has nurse cell phenotype

(Doronkin et al., 2003)

CSN4^k08018/CSN4[+], CSN5^L4032 has egg chamber phenotype

(Doronkin et al., 2003)

CSN5^L4032, Rpn6[+]/Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN5^L4032, Rpn6[+]/Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN5^L4032, Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN5^L4032, Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032 has egg chamber phenotype

(Doronkin et al., 2003)

CSN5^L4032/CSN5[+], Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN5^L4032/CSN5[+], Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032/CSN5[+] has ovary phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032/CSN5[+] has nurse cell phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032/CSN5[+] has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018/CSN4[+], CSN5^L4032 has ovary phenotype

(Doronkin et al., 2003)

CSN4^k08018/CSN4[+], CSN5^L4032 has nurse cell phenotype

(Doronkin et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

CSN5^L4032/+; slmb² germline clones show enhanced phenotypes compared to slmb² germline clones: the proportion of egg chambers with extra germ cells is increased and germaria are enlarged.

(Muzzopappa and Wappner, 2005)

The number of egg chambers with twice the normal number of germ cells in CSN5^ex21/CSN5^L4032 flies, is reduced to 0.6% by CycE^AR95/+. The number of egg chambers with twice the normal number of germ cells in CSN5^ex13/CSN5^L4032 flies, is reduced to 3.4% by CycE⁰⁵²⁰⁶/+. (n>500) The ovaries of CSN5^L4032/CSN4^k08018 transheterozygotes exhibit apoptosis and extra cell division of nurse cells, as well as fused egg chambers. The penetrance of these phenotypes can exceed 50%. Unlike either single heterozygote, nurse cells in CSN5^L4032; Rpn6^k00103 transheterozygous females exhibit ectopic apoptosis, incorrect numbers of mitotic divisions and fusions of neighbouring egg chambers.

(Doronkin et al., 2003)

The fused dorsal appendage phenotype of eggs laid by grk^2B6/+ females is enhanced if the females are also heterozygous for CSN5^L4032. The fused dorsal appendage phenotype of eggs laid by grk^HF/+ females is enhanced if the females are also heterozygous for CSN5^L4032. The fused dorsal appendage phenotype of eggs laid by Egfr^f2/+ females is weakly enhanced if the females are also heterozygous for CSN5^L4032.

(Doronkin et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10301

y¹ w¹¹¹⁸; P{lacW}CSN5^L4032/TM3, Ser¹

Kyoto

111044

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{lacW}CSN5^L4032/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

Comments

Excision of the P{lacW} can be associated with a reversion to wild-type.

(Freilich et al., 1999)

Reversion analysis proved that the P{lacW} is responsible for the lethal phenotype.

(Beaton, 2000.1.31)

Precise excision of the P{lacW} element reverts the mutant phenotype.

(Doronkin et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CH5^L4032

CSN5^L4032

(Wu et al., 2005)

CSN5^P

(Suh et al., 2002)

csn5^L4032

(Ullah et al., 2007)

csn5^P

(Oron et al., 2002)

dch5-1

(Freilich et al., 1999)

l(3)L4032

(Spradling et al., 1999)

l(3)L4032^L4032

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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