FlyBase Allele Report: Dmel\skd[L7062]

General Information

Symbol

Dmel\skd^L7062

Species

D. melanogaster

Name

FlyBase ID

FBal0087026

Feature type

allele

Associated gene

Dmel\skd

Associated Insertion(s)

P{lacW}skd^L7062

Carried in Construct

Also Known As

pap^L7062, l(3)L7062

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}skd^L7062

Cytology

Description

Insertion of P{lacW} into the third skd intron, which falls in the coding region.

(Treisman, 2001)

The P{lacW} insertion is 800bp downstream of the putative ATG start codon.

(Fernandez-Funez et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:21,011,937..21,011,937 [+]

(Branco et al., 2008, Treisman, 2001, Fernandez-Funez et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^30Q.UAS

(Fernandez-Funez et al., 2000)

modeled by Hsap\ATXN1^82Q.UAS

(Fernandez-Funez et al., 2000)

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

exacerbates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

visible | dominant | maternal effect

(Bosveld et al., 2008)

Phenotype Manifest In

adult abdomen | maternal effect

(Bosveld et al., 2008)

embryonic/larval crystal cell

(Milchanowski et al., 2004)

mitotic cell cycle

(Milchanowski et al., 2004)

Detailed Description

Statement

Reference

When heterozygous females are crossed to wild-type males, 22.7% of the heterozygous progeny have abdominal segmentation defects, while 9.5% of the +/+ offspring have defects.

(Bosveld et al., 2008)

No retinal phenotypes are seen in skd^L7062 mutant flies.

(Branco et al., 2008)

The average number of crystal cells per embryo is reduced (to 9.9) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average). Mutant stage 14 embryos show cell cycle defects in the head region.

(Milchanowski et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

skd^L7062 has visible | maternal effect | dominant phenotype, non-enhanceable by MED31¹/MED31[+]

(Bosveld et al., 2008)

Enhancer of

Statement

Reference

skd^L7062 is an enhancer of visible | dominant phenotype of tna¹

(Gutierrez et al., 2003)

Suppressor of

Statement

Reference

skd^L7062/skd[+] is a suppressor of visible | dominant phenotype of Bx¹

(Bejarano et al., 2008)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

skd^L7062 has adult abdomen | maternal effect phenotype, non-enhanceable by MED31¹/MED31[+]

(Bosveld et al., 2008)

Enhancer of

Statement

Reference

skd^L7062/skd[+] is an enhancer of adult epidermis | maternal effect phenotype of MED31¹

(Bosveld et al., 2008)

skd^L7062/skd[+] is an enhancer of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

skd^L7062/skd[+] is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

skd^L7062 is an enhancer of wing phenotype of tna¹

(Gutierrez et al., 2003)

skd^L7062 is an enhancer of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

skd^L7062 is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Fernandez-Funez et al., 2000)

Suppressor of

Statement

Reference

skd^L7062/skd[+] is a suppressor of wing margin phenotype of Bx¹

(Bejarano et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

When MED31¹/MED31¹ females are crossed to skd^L7062/+ males, 54.7% of the MED31¹/skd^L7062 adult progeny have abdominal defects, indicating that zygotic skd^L7062 enhances the MED31¹ phenotype.

No increase in abdominal segmentation defects is seen in the adult progeny of MED31¹/skd^L7062 females compared to the defects of the progeny of skd^L7062/+ females, indicating that MED31¹ is not an enhancer of skd^L7062 when maternally supplied.

(Bosveld et al., 2008)

Xenogenetic Interactions

Statement

Reference

One copy of skd^L7062 enhances the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

One copy of skd^L7062 enhances the retinal phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

Complementation and Rescue Data

Complements

skd^rK760

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10198

y¹ w¹¹¹⁸; P{lacW}skd^L7062/TM3, Ser¹

Notes on Origin

Discoverer

Comments

Complements: l(3)L5541^L5541. Complements: fng^rG554.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

P10198

(Milchanowski et al., 2004)

dMED13¹

(Bosveld et al., 2008)

l(3)L7062

(Treisman, 2001, Spradling et al., 1999)

l(3)L7062^L7062

(BDGP Project Members, 1994-1999)

pap^L7062

(Milchanowski et al., 2004, Fernandez-Funez et al., 2000)

skd¹⁰¹⁹⁸

(Bejarano et al., 2008)

skd^L7062

(Branco et al., 2008)

skd^LOF

(Branco et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close