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General Information
Symbol
Dmel\Cdc27L7123
Species
D. melanogaster
Name
FlyBase ID
FBal0087027
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion 532bp upstream of the ATG codon.

Insertion 519bp upstream of the initiating ATG codon.

The insertion is in the 5'UTR ofthe Cdc27 transcript.

Insertion components
P{lacW}Cdc27L7123
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

aster & neuroblast

chromatin & neuroblast

chromosome & neuroblast

mitotic metaphase & neuroblast

spindle & neuroblast

Detailed Description
Statement
Reference

In Cdc27L7123 mutants, there is a high proportion of cells in a metaphase-like state (a ratio of 8.7 metaphase:anaphase compared to 2.8 in wild-type). Along with the low proportion of cells in anaphase, this suggests that Cdc27L7123 cells arrest or delay in metaphase. The extent of chromosome condensation observed indicates that cells have been delayed in metaphase for a period of time. Cdc27L7123 anaphase cells are frequently disorganised, with chromosomes lagging along the spindle. A low frequency (~3% of mitoses) of polyploid cells are also found in Cdc27L7123 cells. In Cdc27L7123 larval stage brains the sister chromosomes of at least one chromosome are found to be separated in 20% of all mitotic figures. 19% of Cdc27L7123 mitotic cells are found to contain a 4N complement of chromosomes in which there are four sites of hybridization indicating that the sister chromosomes are separated. This is compared to a frequency of 0.4% in wild-type metaphase cells. In mitotic Cdc27L7123 cells, the centromeric regions of the two pairs of major autosomes are present as a series of four separate pairs of dots, indicative of chromosome separation. Approximately 3.9% of Cdc27L7123 cells contain a replicated tetraploid set of chromosomes. Chromatin in Cdc27L7123 mitotic cells is distributed in a broad band. Cdc27L7123 cells exhibit overcondensed chromosomes. Up to 16 separated kinetochores of sister chromosomes are observed in Cdc27L7123 cells, similar to the phenotype observed in polo hypomorphs.

Homozygous flies occasionally survive to adulthood. These escapers have many eye and bristle defects, are invariably sterile and only live for a few days.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
NOT Suppressor of
Statement
Reference

Cdc27[+]/Cdc27L7123 is a non-suppressor of increased cell death phenotype of shtd1

Phenotype Manifest In
NOT Enhanced by
Statement
Reference

Cdc27L7123 has mitotic metaphase & neuroblast & larva phenotype, non-enhanceable by BubR1unspecified

NOT Suppressor of
Statement
Reference

Cdc27[+]/Cdc27L7123 is a non-suppressor of eye phenotype of shtd1

Cdc27[+]/Cdc27L7123 is a non-suppressor of ommatidium phenotype of shtd1

Additional Comments
Genetic Interactions
Statement
Reference

The slight increase in lymph gland size seen in Zfrp8SM206/+ larvae is dominantly enhanced by Cdc27L7123.

Examination of the larval stage central nervous system of BubR1unspecified Cdc27L7123 double mutant shows cells arrested at metaphase at a comparable frequency to the Cdc27L7123 mutant alone. Moreover, the high frequency of anaphases seen in the BubR1unspecified mutant as a result of the malfunctioning checkpoint is replaced in the double mutant by an elevated frequency of metaphase-like figures typical of Cdc27L7123. The appearance of mitotic figures in squashed preparations of BubR1unspecified Cdc27L7123 is indistinguishable from those seen in Cdc27L7123 alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (9)