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General Information
Symbol
Dmel\zipEbr
Species
D. melanogaster
Name
FlyBase ID
FBal0088529
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:
    G24997064A
    Reported nucleotide change:
    G14374A
    Amino acid change:
    R361H | zip-PA; R316H | zip-PB; R276H | zip-PC; R321H | zip-PD; R276H | zip-PE; R284H | zip-PF; R276H | zip-PG; R284H | zip-PH; R329H | zip-PI
    Reported amino acid change:
    R276H
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Amino acid replacement: R277H.
    Nucleotide substitution: G14374A. Amino acid replacement: R276H. Nucleotide coordinates are relative to the sequence record U35816. Amino acid coordinates are relative to the transcript published in FBrf0052857.
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference
    zipEbr/zip02957 transheterozygotes are viable but have malformed wings with varying degrees of severity. Mutants also have rough eyes. In these retina the Mysoin-II system is disorganised . Some rhabdomeres have gaps along their lengths. Moreover, the rhabdomeres are not as straight as in wild-type, but appear to meander slightly. At the retinal floor of mutants, the hexagonal arrangement of the pigment cell pedicels exhibit irregularities, moreover, the retinal floor is not completely flattened but wrinkled in some areas.
    zipEbr/zip33-1 animals have a severe leg malformation phenotype.
    About 70% of zipEbr/zip02957 adults have malformed wings.
    Mutants die as late embryos or early larvae.
    Lethality acts post-embryonically.
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressed by
    Statement
    Reference
    Rho172BH, zipEbr has visible | dominant phenotype, suppressible by Mbs3
    RhoGEF204291, zipEbr has visible | dominant phenotype, suppressible by Mbs3
    Rho172O, zipEbr has visible | dominant phenotype, suppressible | partially by Mbs3
    zip02957/zipEbr has visible phenotype, suppressible | partially by Mbs3/Mbs[+]
    Rok2, zipEbr has visible | dominant phenotype, suppressible by Mbs3
    Enhancer of
    Statement
    Reference
    zip[+]/zipEbr is an enhancer of visible | dominant phenotype of Sb70
    zip[+]/zipEbr is an enhancer of visible | dominant phenotype of Sb63b
    zip[+]/zipEbr is an enhancer of visible | dominant phenotype of br1
    Suppressor of
    Statement
    Reference
    zipEbr is a suppressor of lethal | recessive | larval stage phenotype of MoeG0323
    zip[+]/zipEbr is a suppressor of lethal | embryonic stage phenotype of Scer\GAL4arm.PS, RokUAS.cMa
    zip[+], zipEbr, Scer\GAL4[-] is a suppressor of lethal | maternal effect | embryonic stage phenotype of MbsEP3727/Df(3L)th117, Mbs3
    zip[+], zipEbr, Scer\GAL4[-] is a suppressor of lethal | maternal effect | embryonic stage phenotype of Mbs3/Df(3L)th117, MbsEP3727
    Other
    Statement
    Reference
    Sb70, zip[+]/zipEbr has visible | dominant phenotype
    Rok2, zip[+]/zipEbr has visible | dominant phenotype
    RhoGEF204291/RhoGEF2[+], zipEbr has visible phenotype
    rok[+]/Rok2, zipEbr has visible phenotype
    Rok1, zip[+]/zipEbr has visible | dominant phenotype
    E(zip)12-5[+]/E(zip)12-512-5, zipEbr has visible phenotype
    E(zip)18-5[+]/E(zip)18-518-5, zipEbr has visible phenotype
    E(zip)31-631-6/E(zip)31-6[+], zipEbr has visible phenotype
    Rho112-6/Rho1[+], zipEbr has visible phenotype
    RhoGEF21.1/RhoGEF2[+], zipEbr has visible phenotype
    RhoGEF211-3/RhoGEF2[+], zipEbr has visible phenotype
    RhoGEF24.1/RhoGEF2[+], zipEbr has visible phenotype
    Sb[+]/Sb70, zipEbr has visible phenotype
    rok[+]/Rok1, zipEbr has visible phenotype
    E(br)121Ebr121/E(br)121[+], zipEbr has visible phenotype
    E(br)160[+]/E(br)160Ebr160, zipEbr has visible phenotype
    E(br)444[+]/E(br)444Ebr444, zipEbr has visible phenotype
    E(br)65Ebr65/E(br)65[+], zipEbr has visible phenotype
    Rho1Ebr233/Rho1[+], zipEbr has visible phenotype
    Rho1[+]/Rho1Ebr246, zipEbr has visible phenotype
    Sb[+]/SbEbr20, zipEbr has visible phenotype
    Sb[+]/SbEbr48, zipEbr has visible phenotype
    Phenotype Manifest In
    NOT Enhanced by
    Statement
    Reference
    zipEbr has phenotype, non-enhanceable by Eip74EFDL-1
    zipEbr has phenotype, non-enhanceable by Eip74EFneo24
    zipEbr has phenotype, non-enhanceable by Khc8
    zipEbr has phenotype, non-enhanceable by l(2)52FbD2.33
    zipEbr has phenotype, non-enhanceable by l(2)52FcD8.13
    zipEbr has phenotype, non-enhanceable by l(2)52FdE3.27
    zipEbr has phenotype, non-enhanceable by l(2)52FeE6.17
    Suppressed by
    Statement
    Reference
    RhoGEF204291, zipEbr has wing phenotype, suppressible by Mbs3
    Rho172O, zipEbr has wing phenotype, suppressible | partially by Mbs3
    Rho172BH, zipEbr has wing phenotype, suppressible by Mbs3
    zip02957/zipEbr has wing phenotype, suppressible | partially by Mbs3/Mbs[+]
    Rok2, zipEbr has wing phenotype, suppressible by Mbs3
    Enhancer of
    Statement
    Reference
    zip[+]/zipEbr is an enhancer of leg phenotype of Sb70
    zip[+]/zipEbr is an enhancer of leg phenotype of br1
    zip[+]/zipEbr is an enhancer of leg phenotype of Sb63b
    Other
    Statement
    Reference
    Sb70, zip[+]/zipEbr has wing phenotype
    Rok2, zip[+]/zipEbr has leg phenotype
    Rok1, zip[+]/zipEbr has leg phenotype
    Rok1, zip[+]/zipEbr has wing phenotype
    RhoGEF211-3, zip[+]/zipEbr has leg phenotype
    RhoGEF211-3, zip[+]/zipEbr has wing phenotype
    Rho112-6, zip[+]/zipEbr has leg phenotype
    Rho112-6, zip[+]/zipEbr has wing phenotype
    SbEbr536, zip[+]/zipEbr has leg phenotype
    E(br)65Ebr65, zip[+]/zipEbr has leg phenotype
    Rho1Ebr233, zip[+]/zipEbr has leg phenotype
    SbEbr48, zip[+]/zipEbr has leg phenotype
    SbEbr448, zip[+]/zipEbr has leg phenotype
    SbEbr623, zip[+]/zipEbr has leg phenotype
    Rho1Ebr246, zip[+]/zipEbr has leg phenotype
    SbEbr20, zip[+]/zipEbr has leg phenotype
    Rho172BH/Rho1[+], zipEbr has wing phenotype
    Rho172O/Rho1[+], zipEbr has wing phenotype
    RhoGEF204291/RhoGEF2[+], zipEbr has wing phenotype
    rok[+]/Rok2, zipEbr has wing phenotype
    Rho1J3.8, zip[+]/zipEbr has leg phenotype
    Rho172O, zip[+]/zipEbr has leg phenotype
    Rho172F, zip[+]/zipEbr has leg phenotype
    Rho172F, zip[+]/zipEbr has wing phenotype
    Rho1E3.10, zip[+]/zipEbr has leg phenotype
    Rho1E3.10, zip[+]/zipEbr has wing phenotype
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    A zip2/zipEbr mutant background (which reducing tension in cells) induces cell death at the anterior boundary when fweScer\UAS.P\T.Lose-A.C.T:Ivir\HA1 is expressed under the control of Scer\GAL4dpp.PU in the anterior compartment of the wing disc.
    The frequency of the malformed leg phenotype seen in Sb63b/+ heterozygotes (8%) is increased if the flies are also heterozygous for zipEbr (63%). The frequency of the malformed leg phenotype seen in Sb70/+ heterozygotes (7%) is increased if the flies are also heterozygous for zipEbr (96%). The frequency of the malformed leg phenotype seen in br1/Y males (1%) is increased if the flies are also heterozygous for zipEbr (56%).
    zipEbr shows no interaction with sqhunspecified (assayed in terms of a malformed leg phenotype). zipEbr shows a strong interaction (at least 50% of double heterozygotes have at least one malformed leg) with the following mutations: RhoGEF211-3, E(zip)12-512-5 and Rho112-6. zipEbr shows a moderate interaction (25-49% of double heterozygotes have at least one malformed leg) with the following mutations: E(zip)18-518-5. zipEbr shows a weak interaction (5-24% of double heterozygotes have at least one malformed leg) with the following mutations: E(zip)31-631-6. zipEbr shows a strong interaction (at least 50% of double heterozygotes have at least one malformed wing) with the following mutations: rok1, Sb70, RhoGEF211-3, E(zip)12-512-5, Rho112-6 and E(zip)31-631-6. zipEbr shows a moderate interaction (25-49% of double heterozygotes have at least one malformed wing) with the following mutations: E(zip)18-518-5.
    The fraction of flies showing a malformed leg phenotype in at least one leg, for zipEbr in double heterozygous combination with one of the following alleles is - SbEbr20 : 46%, SbEbr48 : 30%, SbEbr228 : 14%, SbEbr448 : 57%, SbEbr536 : 33%, SbEbr623 : 26%, Rho1Ebr233 : 93%, Rho1Ebr246 : 83%, bsEbr292 : 2%, E(br)24Ebr24 : 9%, E(br)65Ebr65 : 67%, E(br)155Ebr155 : 7%, E(br)165Ebr165 : 6%, E(br)333Ebr333 : 4%, E(br)72Ebr72 : 4%, E(br)121Ebr121 : 82%, E(br)160Ebr160 : 20%, E(br)187Ebr187 : 6%, E(br)420Ebr420 : 15% and E(br)444Ebr444 : 84%.
    The lethality of Mbs mutant embryos derived from MbsEP3727/Df(3L)th117 females mated to Mbs3/+ males is suppressed by zipEbr/+.
    27% of RhoGEF24.1/zipEbr double heterozygotes have a malformed leg phenotype, which shows variable expressivity. In less severe cases, there is a dent in the femur or tibia. Flies with malformed legs often also show wing defects. Weak phenotypes include a broadening of the wing blade and folding back of the distal portion of the wing blade. In the most severe cases, wings are greatly reduced in size. 38% of RhoGEF204291/zipEbr double heterozygotes have a malformed leg phenotype. 33% of RhoGEF21.1/zipEbr double heterozygotes have a malformed leg phenotype. 100% of Rho1J3.8/zipEbr double heterozygotes have a malformed leg phenotype. 100% of Rho1E3.10/zipEbr double heterozygotes have a malformed leg phenotype, which shows variable expressivity. In more severe cases, a dent and twisting of the femur is apparent, along with shortening and thickening of the tibia. Notching of the posterior region of the wing blade is also seen. 96% of Rho172F/zipEbr double heterozygotes have a malformed leg phenotype. Wing defects are also seen. Weak phenotypes include a broadening of the wing blade and folding back of the distal portion of the wing blade. In more severe cases, wings lacking adhesion between the wing blade bilayer and any apparent wing venation are seen. 92% of Rho172O/zipEbr double heterozygotes have a malformed leg phenotype. 90% of Df(2R)Jp4/zipEbr double heterozygotes have a malformed leg phenotype. 98% of Df(2R)Jp8/zipEbr double heterozygotes have a malformed leg phenotype.
    Produces malformed phenotype when in double heterozygosis with second site zip non-complementing mutants. Shows a higher level of penetrance with second site non-complementers than does zipmhc-c6.1.
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (6)
    References (19)