Allele Dmel\numb^{fl.Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\numb^{fl.Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0088741
Feature type	allele	Associated gene	Dmel\numb

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Yaich et al., 1998) in vitro construct - regulatory fusion (Yaich et al., 1998, Park et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length wild-type numb cDNA, tagged at the C-terminus with T:Hsap\MYC, is expressed under the control of Scer\UAS regulatory sequences. (Yaich et al., 1998)
Carried in construct	P{UAS-numb.T:Myc.Y} (Langevin et al., 2005, Han and Bodmer, 2003, Su et al., 1999, Park et al., 1998, Yaich et al., 1998, Thomas and van Meyel, 2007, Smith et al., 2007, Wirtz-Peitz et al., 2008, Orgogozo et al., 2002, Tong et al., 2010)
Tagged with	T:Hsap\MYC (Yaich et al., 1998, Park et al., 1998, Su et al., 1999, Han and Bodmer, 2003, Langevin et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4¹⁸⁹ (Yaich et al., 1998)
Phenotype Manifest In
	embryonic pericardial cell, with Scer\GAL4^eve.eme (Han and Bodmer, 2003) macrochaeta, with Scer\GAL4¹⁸⁹ (Yaich et al., 1998) pericardial cell, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Park et al., 1998) wing sensillum, with Scer\GAL4^bbg-C96 (Yaich et al., 1998)
Detailed Description
	Statement Reference When numb^{fl.Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^eve.eme a loss of eve expressing pericardial cells is seen. Instead two pairs of DA1 and DO2 muscle founders are seen. (Han and Bodmer, 2003) spi¹ embryos in which numb^{fl.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^how-24B and Scer\GAL4^twi.PG lack the eve-expressing pericardial cells and DA1 muscles. (Su et al., 1999) Embryos expressing numb^{fl.Scer\UAS.T:Hsap\MYC} under the control of both Scer\GAL4^how-24B and Scer\GAL4^twi.PG form DA1 muscles but the number of eve-expressing pericardial cells is drastically reduced. (Park et al., 1998) 76-100% of macrochaetae are missing in most lines of flies expressing numb^{fl.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4¹⁸⁹. Overexpression of numb^{fl.Scer\UAS.T:Hsap\MYC} using Scer\GAL4^C96 results in twinning of bristles on the wing margin. (Yaich et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference numb^{fl.Scer\UAS.T:Hsap\MYC}/Scer\GAL4¹⁴⁰⁷ is a suppressor of neuroanatomy defective phenotype of Scer\GAL4¹⁴⁰⁷, aPKC^{CAAXWT.Scer\UAS} (Wirtz-Peitz et al., 2008)
Phenotype Manifest In
Suppressor of
Statement Reference numb^{fl.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^eve.eme is a suppressor \| partially of A1-7 dorsal acute muscle 1 phenotype of N^{int.G.Scer\UAS}, Scer\GAL4^eve.eme (Han and Bodmer, 2003) numb^{fl.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^eve.eme is a suppressor \| partially of mesothoracic dorsal acute muscle 1 phenotype of N^{int.G.Scer\UAS}, Scer\GAL4^eve.eme (Han and Bodmer, 2003) numb^{fl.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^eve.eme is a suppressor \| partially of metathoracic dorsal acute muscle 1 phenotype of N^{int.G.Scer\UAS}, Scer\GAL4^eve.eme (Han and Bodmer, 2003) numb^{fl.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^eve.eme is a suppressor \| partially of pericardial cell \| ectopic phenotype of N^{int.G.Scer\UAS}, Scer\GAL4^eve.eme (Han and Bodmer, 2003) numb^{fl.Scer\UAS.T:Hsap\MYC}/Scer\GAL4¹⁴⁰⁷ is a suppressor of embryonic/larval brain \| larval stage phenotype of Scer\GAL4¹⁴⁰⁷, aPKC^{CAAXWT.Scer\UAS} (Wirtz-Peitz et al., 2008) numb^{fl.Scer\UAS.T:Hsap\MYC}/Scer\GAL4¹⁴⁰⁷ is a suppressor of embryonic/larval neuroblast phenotype of Scer\GAL4¹⁴⁰⁷, aPKC^{CAAXWT.Scer\UAS} (Wirtz-Peitz et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference The overproliferation of neuroblasts seen in the larval brain in animals expressing aPKC[CAAXWT.Scer\UAS] under the control of Scer\GAL4[1407] at 29[o]C is suppressed by co-expression of numb[fl.Scer\UAS.T:Hsap\MYC]. (Wirtz-Peitz et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	numb^{fl.Scer\UAS.T:Hsap\MYC} numb^fl.UAS.T:Myc
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Haenfler et al., 2012, Dev. Biol. 365(1): 219--228 Cortical aPKC kinase activity distinguishes neural stem cells from progenitor cells by ensuring asymmetric segregation of Numb. [FBrf0218950] Tong et al., 2010, Mol. Biol. Cell 21(5): 802--810 Numb independently antagonizes sanpodo membrane targeting and notch signaling in Drosophila sensory organ precursor cells. [FBrf0210112] Wirtz-Peitz et al., 2008, Cell 135(1): 161--173 Linking cell cycle to asymmetric division: Aurora-A phosphorylates the Par complex to regulate Numb localization. [FBrf0206088] Smith et al., 2007, EMBO J. 26(2): 468--480 aPKC-mediated phosphorylation regulates asymmetric membrane localization of the cell fate determinant Numb. [FBrf0194071] Thomas and van Meyel, 2007, Development 134(3): 591--600 The glycosyltransferase Fringe promotes Delta-Notch signaling between neurons and glia, and is required for subtype-specific glial gene expression. [FBrf0192828] Langevin et al., 2005, Curr. Biol. 15(10): 955--962 Lethal giant larvae controls the localization of notch-signaling regulators numb, neuralized, and Sanpodo in Drosophila sensory-organ precursor cells. [FBrf0187315] Han and Bodmer, 2003, Development 130(13): 3039--3051 Myogenic cells fates are antagonized by Notch only in asymmetric lineages of the Drosophila heart, with or without cell division. [FBrf0160590] Orgogozo et al., 2002, Development 129(20): 4677--4684 Binary cell death decision regulated by unequal partitioning of Numb at mitosis. [FBrf0151928] Su et al., 1999, Development 126(14): 3241--3251 The Drosophila homeobox genes zfh-1 and even-skipped are required for cardiac-specific differentiation of a numb-dependent lineage decision. [FBrf0109098] Park et al., 1998, Mech. Dev. 75(1,2): 117--126 Mesodermal cell fate decisions in Drosophila are under the control of the lineage genes numb, Notch and sanpodo. [FBrf0104706] Yaich et al., 1998, J. Biol. Chem. 273(17): 10381--10388 Functional analysis of the numb phosphotyrosine-binding domain using site-directed mutagenesis. [FBrf0102421]
Supplementary material	Wirtz-Peitz et al., 2008, Cell 135(1): Supplemental Data. [FBrf0211096]

Allele Dmel\numbfl.Scer\UAS.T:Hsap\MYC

Allele Dmel\numb^{fl.Scer\UAS.T:Hsap\MYC}