Allele Dmel\mof²

General Information
Symbol	Dmel\mof²	Species	D. melanogaster
Name		FlyBase ID	FBal0088802
Feature type	allele	Associated gene	Dmel\mof

Map ( GBrowse )
Allele class
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	New/Merged/Split Record This record was either newly added to FlyBase for this release or is the result of merging or splitting existing records in this release
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation X:5,769,675..5,769,675 comment=Site of nucleic acid change inferred from report that a truncated protein of 370 amino acids is made in the mutant. evidence=experimental pr_change=Q371@\|mof-PA na_change=C5769675T (Gu et al., 1998)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Truncated protein of 370 instead of 827 amino acids. (Gu et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Conrad et al., 2012) lethal \| male (Conrad et al., 2012) partially lethal - majority die \| female (Conrad et al., 2012) short lived \| female (Conrad et al., 2012)
Phenotype Manifest In

Detailed Description
	Statement Reference Mutant males show full lethality, whereas the number of females reaching adulthood is reduced approximately 2-fold compared to wild type. Most mutant female adults are sterile. The average lifespan of mutant females is reduced to 8 days compared to 37 days in controls. (Conrad et al., 2012)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference mof[+]/mof² is an enhancer of partially lethal - majority die \| dominant \| female phenotype of sc^sisB-1, sisA[+]/sisA¹ (Gladstein et al., 2010)
NOT Enhancer of
Statement Reference mof[+]/mof² is a non-enhancer of partially lethal - majority die phenotype of Hsap\HTT^{Q93.ex1p.Scer\UAS}, Scer\GAL4^elav-C155 (Bodai et al., 2012)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference One paternally-derived copy of mof[2] reduces the viability of sc[sisB-1] sisA[1]/+ females. (Gladstein et al., 2010)
Xenogenetic Interactions
Statement Reference One copy of mof[2] does not enhance the reduction in eclosion rate seen when Hsap\HTT[Q93.ex1p.Scer\UAS] is expressed under the control of Scer\GAL4[elav-C155]. (Bodai et al., 2012)
Complementation & Rescue Data
Rescued by	mof² is rescued by mof^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PU (Conrad et al., 2012)
Partially rescued by	mof² is partially rescued by mof^{Δ1.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PU (Conrad et al., 2012) mof² is partially rescued by mof^{Δ2.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PU (Conrad et al., 2012) mof² is partially rescued by mof^{Δ3.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PU (Conrad et al., 2012) mof² is partially rescued by mof^{ΔN.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PU (Conrad et al., 2012)
Not rescued by	mof² is not rescued by mof^{W426G.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PU (Conrad et al., 2012) mof² is not rescued by mof^{Y416D.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^arm.PU (Conrad et al., 2012) mof² is not rescued by Scer\GAL4^arm.PU/mof^{ΔZn.Scer\UAS.T:Ivir\HA1} (Conrad et al., 2012)
Comments	Expression of mof[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[arm.PU] completely rescues the lethality of mof[2] males. Expression of either mof[ΔZn.Scer\UAS.T:Ivir\HA1], mof[W426G.Scer\UAS.T:Ivir\HA1] or mof[Y416D.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[arm.PU] fails to rescue the lethality of mof[2] males. The lethality of mof[2] males is partially rescued by expression of either mof[Δ1.Scer\UAS.T:Ivir\HA1] (49.6% rescue), mof[Δ2.Scer\UAS.T:Ivir\HA1] (59.3% rescue), mof[Δ3.Scer\UAS.T:Ivir\HA1] (42.5% rescue) or mof[ΔN.Scer\UAS.T:Ivir\HA1] (11.2% rescue) under the control of Scer\GAL4[arm.PU]. (Conrad et al., 2012)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	mof² (Gelbart et al., 2009, Gladstein et al., 2010, Conrad et al., 2012, Bodai et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Bodai et al., 2012, Neurodegener. Dis. 9(2): 104--106 Pcaf Modulates Polyglutamine Pathology in a Drosophila Model of Huntington's Disease. [FBrf0217401] Conrad et al., 2012, Dev. Cell 22(3): 610--624 The MOF Chromobarrel Domain Controls Genome-wide H4K16 Acetylation and Spreading of the MSL Complex. [FBrf0217803] Gladstein et al., 2010, PLoS Genet. 6(7): e1001041 Requirement of male-specific dosage compensation in Drosophila females-implications of early X chromosome gene expression. [FBrf0211448] Gelbart et al., 2009, Nat. Struct. Mol. Biol. 16(8): 825--832 Drosophila MSL complex globally acetylates H4K16 on the male X chromosome for dosage compensation. [FBrf0208599] Rattner and Meller, 2004, Genetics 166(4): 1825--1832 Drosophila Male-Specific Lethal 2 Protein Controls Sex-Specific Expression of the roX Genes. [FBrf0174697] Meller, 2003, Mech. Dev. 120(7): 759--767 Initiation of dosage compensation in Drosophila embryos depends on expression of the roX RNAs. [FBrf0162226] Gu et al., 2000, EMBO J. 19(19): 5202--5211 Targeting the chromatin-remodeling MSL complex of Drosophila to its sites of action on the X chromosome requires both acetyl transferase and ATPase activities. [FBrf0129838] Gu et al., 1998, Dev. Genet. 22(1): 56--64 Targeting of MOF, a putative histone acetyl transferase, to the X chromosome of Drosophila melanogaster. [FBrf0101916]

Allele Dmel\mof2

Allele Dmel\mof²