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General Information
Symbol
Dmel\fz2ECD.GPI.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0089261
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-GPI-Dfz2, UAS-DFz2-GPI
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of the extracellular domain of fz2 with a GPI linkage. The protein is also tagged with Tag:MYC.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of fz2ECD.GPI.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4dome-PG14 results in mislocalisation of hemocyte precursors from the medullary zone into the cortical zone of the lymph gland, and differentiation of hemocyte precursors into plasmatocytes is observed. Expression of fz2ECD.GPI.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Antp-10 causes a significant reduction in the number of cells of the posterior signalling center of the lymph gland. There is a reduction in the pool of hemocyte precursors, and the overall size of the lymph gland is reduced during third instar. Expression of fz2ECD.GPI.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4lz-gal4 results in a significant reduction in the number of crystal cells.
Ectopic expression of fz2ECD.GPI.Scer\UAS.T:Hsap\MYC, under the control of Scer\GAL4vg.PM generates ectopic wing margin bristles.
Expression of fz2ECD.GPI.Scer\UAS.T:Hsap\MYC in the retina behind the furrow using Scer\GAL4GMR.PF results in the projection of ventral R axons into the dorsal lamina.
Photoreceptor apoptosis at the periphery of developing eyes at 42 hours after puparium formation is almost completely eliminated in fz2ECD.GPI.Scer\UAS.T:Hsap\MYC; Scer\GAL4GMR.PF animals.
Expression of fz2ECD.GPI.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 results in a phenotype similar to that produced by overexpression of sgg; the number of 1o, 2o and 3o denticles is unaffected, but the number of 4o denticles is severely reduced in the dorsal epidermis of the embryo (in wild-type embryos each parasegment contains 4 types of cells in the dorsal epidermis; the 1o, 2o and 3o cells in the anterior half of the parasegment and the 4o cells in the posterior half of the parasegment).
When driven by Scer\GAL4C96, almost all flies exhibit wings with anterior terminal nicking.
Embryos expressing fz2ECD.GPI.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 show partially penetrant defects in the NB4-2 -> GMC-1 -> RP2/sib lineage and show defects in row 6 neuroblast formation. Temperature shift experiments in which fz2ECD.GPI.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4hs.PB at different times during embryogenesis indicate that the highest penetrance of RP2/sib lineage defects in these embryos occurs when fz2ECD.GPI.Scer\UAS.T:Hsap\MYC is expressed during midstage 8.
Flies expressing fz2ECD.GPI.Scer\UAS.T:Hsap\MYC in the wing pouch under the control of Scer\GAL4unspecified have severe notching of the wings.
Scer\GAL4unspecified-mediated expression results in loss of the wing margin.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Enhances the wing nicking phenotype seen in mamN.Scer\UAS, Scer\GAL4C96 flies producing a wide range of phenotypes, including severe loss of blade material.
The penetrance of the phenotype seen in embryos expressing fz2ECD.GPI.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 is enhanced if the embryos are also heterozygous for wgunspecified.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments
Carried in a plasmid and transfected into S2 cells to study the effects of the expressed membrane-bound fz2 on wg internalization.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
fz2ECD.GPI.Scer\UAS.T:Hsap\MYC
fz2ECD.GPI.UAS.T:Myc
fz2ECD.GPI.UAS.Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (25)