Allele Dmel\fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0089261
Feature type	allele	Associated gene	Dmel\fz2

Allele class
Mutagen	in vitro construct - coding region fusion, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Nusse et al., 1997, Cadigan et al., 1998) in vitro construct - regulatory fusion (Nusse et al., 1997, Cadigan et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of the extracellular domain of fz2 with a GPI linkage. The protein is also tagged with T:Hsap\MYC. (Cadigan et al., 1998) GPI-linked extracellular domain of fz2 is cloned into pUAST vector. (Nusse et al., 1997)
Carried in construct	P{UAS-fz2(ECD)-GPI} (Yoshikawa et al., 2003, Sudarsan et al., 2001, Ghazi et al., 2003, Buratovich and Wilder, 2001, Packard et al., 2002, Lin et al., 2004, Jaiswal et al., 2006, Giraldez and Cohen, 2003, Buratovich et al., 2000, Strigini and Cohen, 2000, Helms et al., 1999, Bajpai et al., 2004, Dubois et al., 2001, Bhat, 1998, Cadigan et al., 1998, Rousset et al., 2001, Brennecke et al., 2003, Nusse et al., 1997, Yan et al., 2009, Yan et al., 2009, Sato et al., 2006, Sinenko et al., 2009, Kleinschmit et al., 2010)
Tagged with	T:Hsap\MYC (Nusse et al., 1997, Cadigan et al., 1998, Bhat, 1998, Helms et al., 1999, Buratovich et al., 2000, Strigini and Cohen, 2000, Rousset et al., 2001, Dubois et al., 2001, Sudarsan et al., 2001, Buratovich and Wilder, 2001, Packard et al., 2002, Yoshikawa et al., 2003, Brennecke et al., 2003, Ghazi et al., 2003, Giraldez and Cohen, 2003, Lin et al., 2004, Bajpai et al., 2004, Piddini et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell death \| pupal stage, with Scer\GAL4^GMR.PF (Lin et al., 2004) neuroanatomy defective, with Scer\GAL4^GMR.PF (Sato et al., 2006) visible, with Scer\GAL4^unspecified (Cadigan et al., 1998, Nusse et al., 1997)
Phenotype Manifest In
	crystal cell \| third instar larval stage, with Scer\GAL4^lz-gal4 (Sinenko et al., 2009) dorsal denticle, with Scer\GAL4^da.G32 (Buratovich et al., 2000) embryonic/larval lymph gland \| third instar larval stage, with Scer\GAL4^Antp-10 (Sinenko et al., 2009) embryonic epidermis \| dorsal, with Scer\GAL4^da.G32 (Buratovich et al., 2000) eye photoreceptor cell, with Scer\GAL4^GMR.PF (Sato et al., 2006) eye photoreceptor cell \| P-stage, with Scer\GAL4^GMR.PF (Lin et al., 2004) lamina, with Scer\GAL4^GMR.PF (Sato et al., 2006) lymph gland derived hemocyte \| third instar larval stage, with Scer\GAL4^dome-PG14 (Sinenko et al., 2009) neuron, with Scer\GAL4^GMR.PF (Sato et al., 2006) RP2 motor neuron, with Scer\GAL4^da.G32 (Bhat, 1998) RP2 motor neuron, with Scer\GAL4^hs.PB (Bhat, 1998) wing, with Scer\GAL4^bbg-C96 (Helms et al., 1999) wing, with Scer\GAL4^unspecified (Cadigan et al., 1998, Nusse et al., 1997)
Detailed Description
	Statement Reference Expression of fz2[ECD.GPI.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[dome-PG14] results in mislocalisation of hemocyte precursors from the medullary zone into the cortical zone of the lymph gland, and differentiation of hemocyte precursors into plasmatocytes is observed. Expression of fz2[ECD.GPI.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[Antp-10] causes a significant reduction in the number of cells of the posterior signalling center of the lymph gland. There is a reduction in the pool of hemocyte precursors, and the overall size of the lymph gland is reduced during third instar. Expression of fz2[ECD.GPI.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[lz-gal4] results in a significant reduction in the number of crystal cells. (Sinenko et al., 2009) Ectopic expression of fz2[ECD.GPI.Scer\UAS.T:Hsap\MYC], under the control of Scer\GAL4[vg.PM] generates ectopic wing margin bristles. (Jaiswal et al., 2006) Expression of fz2[ECD.GPI.Scer\UAS.T:Hsap\MYC] in the retina behind the furrow using Scer\GAL4[GMR.PF] results in the projection of ventral R axons into the dorsal lamina. (Sato et al., 2006) Photoreceptor apoptosis at the periphery of developing eyes at 42 hours after puparium formation is almost completely eliminated in fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^GMR.PF animals. (Lin et al., 2004) Expression of fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^da.G32 results in a phenotype similar to that produced by overexpression of sgg; the number of 1^o, 2^o and 3^o denticles is unaffected, but the number of 4^o denticles is severely reduced in the dorsal epidermis of the embryo (in wild-type embryos each parasegment contains 4 types of cells in the dorsal epidermis; the 1^o, 2^o and 3^o cells in the anterior half of the parasegment and the 4^o cells in the posterior half of the parasegment). (Buratovich et al., 2000) When driven by Scer\GAL4^C96, almost all flies exhibit wings with anterior terminal nicking. (Helms et al., 1999) Embryos expressing fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^da.G32 show partially penetrant defects in the NB4-2 -> GMC-1 -> RP2/sib lineage and show defects in row 6 neuroblast formation. Temperature shift experiments in which fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^hs.PB at different times during embryogenesis indicate that the highest penetrance of RP2/sib lineage defects in these embryos occurs when fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} is expressed during midstage 8. (Bhat, 1998) Flies expressing fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} in the wing pouch under the control of Scer\GAL4^unspecified have severe notching of the wings. (Cadigan et al., 1998) Scer\GAL4^unspecified-mediated expression results in loss of the wing margin. (Nusse et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Scer\GAL4^bbg-C96/fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, mam^N.Scer\UAS (Helms et al., 1999)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^da.G32, fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} has RP2 motor neuron phenotype, enhanceable by wg[+]/wg^unspecified (Bhat, 1998)
Suppressor of
Statement Reference Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF, fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} is a suppressor of interommatidial bristle \| cell autonomous phenotype of wg^sev.PC (Rousset et al., 2001)
NOT Suppressor of
Statement Reference Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF, fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, dsh^Scer\UAS.cAa (Rousset et al., 2001) Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF, fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} is a non-suppressor of interommatidial bristle phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, dsh^Scer\UAS.cAa (Rousset et al., 2001)
Other
Statement Reference Scer\GAL4^dome-PG14, fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}, fz^Scer\UAS.N has lymph gland derived hemocyte \| third instar larval stage phenotype (Sinenko et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Enhances the wing nicking phenotype seen in mam^N.Scer\UAS, Scer\GAL4^C96 flies producing a wide range of phenotypes, including severe loss of blade material. (Helms et al., 1999) The penetrance of the phenotype seen in embryos expressing fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^da.G32 is enhanced if the embryos are also heterozygous for wg^unspecified. (Bhat, 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in a plasmid and transfected into S2 cells to study the effects of the expressed membrane-bound fz2 on wg internalization. (Piddini et al., 2005)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC} fz2^{ECD.GPI.UAS.T:Myc}
Name Synonym
Secondary FlyBase IDs

References ( 24 )

Generate a list of	All references relating to this allele ( 24 )

List References by type	Research paper ( 22 ) Supplementary material ( 1 ) Review ( 1 )
Recent research papers (0)
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Recent reviews (0)
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Allele Dmel\fz2ECD.GPI.Scer\UAS.T:Hsap\MYC

Allele Dmel\fz2^{ECD.GPI.Scer\UAS.T:Hsap\MYC}