|Feature type||allele||Associated gene||Dmel\fz2|
|Mutagen||in vitro construct - coding region fusion, in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Construct: Scer\UAS regulatory sequences drive expression of the extracellular domain of fz2 with a GPI linkage. The protein is also tagged with T:Hsap\MYC.
|Carried in construct|
(Yoshikawa et al., 2003, Sudarsan et al., 2001, Ghazi et al., 2003, Buratovich and Wilder, 2001, Packard et al., 2002, Lin et al., 2004, Jaiswal et al., 2006, Giraldez and Cohen, 2003, Buratovich et al., 2000, Strigini and Cohen, 2000, Helms et al., 1999, Bajpai et al., 2004, Dubois et al., 2001, Bhat, 1998, Cadigan et al., 1998, Rousset et al., 2001, Brennecke et al., 2003, Nusse et al., 1997, Yan et al., 2009, Yan et al., 2009, Sato et al., 2006, Sinenko et al., 2009, Kleinschmit et al., 2010)
(Nusse et al., 1997, Cadigan et al., 1998, Bhat, 1998, Helms et al., 1999, Buratovich et al., 2000, Strigini and Cohen, 2000, Rousset et al., 2001, Dubois et al., 2001, Sudarsan et al., 2001, Buratovich and Wilder, 2001, Packard et al., 2002, Yoshikawa et al., 2003, Brennecke et al., 2003, Ghazi et al., 2003, Giraldez and Cohen, 2003, Lin et al., 2004, Bajpai et al., 2004, Piddini et al., 2005)
|Phenotype Manifest In|
Expression of fz2[ECD.GPI.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[dome-PG14] results in mislocalisation of hemocyte precursors from the medullary zone into the cortical zone of the lymph gland, and differentiation of hemocyte precursors into plasmatocytes is observed. Expression of fz2[ECD.GPI.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[Antp-10] causes a significant reduction in the number of cells of the posterior signalling center of the lymph gland. There is a reduction in the pool of hemocyte precursors, and the overall size of the lymph gland is reduced during third instar. Expression of fz2[ECD.GPI.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[lz-gal4] results in a significant reduction in the number of crystal cells.
Ectopic expression of fz2[ECD.GPI.Scer\UAS.T:Hsap\MYC], under the control of Scer\GAL4[vg.PM] generates ectopic wing margin bristles.
Expression of fz2[ECD.GPI.Scer\UAS.T:Hsap\MYC] in the retina behind the furrow using Scer\GAL4[GMR.PF] results in the projection of ventral R axons into the dorsal lamina.
Photoreceptor apoptosis at the periphery of developing eyes at 42 hours after puparium formation is almost completely eliminated in fz2ECD.GPI.Scer\UAS.T:Hsap\MYC; Scer\GAL4GMR.PF animals.
Expression of fz2ECD.GPI.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 results in a phenotype similar to that produced by overexpression of sgg; the number of 1o, 2o and 3o denticles is unaffected, but the number of 4o denticles is severely reduced in the dorsal epidermis of the embryo (in wild-type embryos each parasegment contains 4 types of cells in the dorsal epidermis; the 1o, 2o and 3o cells in the anterior half of the parasegment and the 4o cells in the posterior half of the parasegment).
When driven by Scer\GAL4C96, almost all flies exhibit wings with anterior terminal nicking.
Embryos expressing fz2ECD.GPI.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 show partially penetrant defects in the NB4-2 -> GMC-1 -> RP2/sib lineage and show defects in row 6 neuroblast formation. Temperature shift experiments in which fz2ECD.GPI.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4hs.PB at different times during embryogenesis indicate that the highest penetrance of RP2/sib lineage defects in these embryos occurs when fz2ECD.GPI.Scer\UAS.T:Hsap\MYC is expressed during midstage 8.
Flies expressing fz2ECD.GPI.Scer\UAS.T:Hsap\MYC in the wing pouch under the control of Scer\GAL4unspecified have severe notching of the wings.
|Phenotype Manifest In|
|NOT Suppressor of|
Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, fz2ECD.GPI.Scer\UAS.T:Hsap\MYC is a non-suppressor of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, dshScer\UAS.cAa
Enhances the wing nicking phenotype seen in mamN.Scer\UAS, Scer\GAL4C96 flies producing a wide range of phenotypes, including severe loss of blade material.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
|Secondary FlyBase IDs|
|References ( 24 )|
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|List References by type|
|Recent research papers (0)|
|All research papers listed in FlyBase were published before 2011|
|Recent reviews (0)|
|All reviews listed in FlyBase were published before 2011|