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General Information
Symbol
Dmel\dsUAO71
Species
D. melanogaster
Name
FlyBase ID
FBal0089339
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dsUA071
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

dsYAC41/dsUAO71 transheterozygous adults show hair polarity defects in abdominal bristles.

Expression of dsΔECD.Scer\UAS under the control of Scer\GAL4hh.PU in a dsUAO71 mutant background produces wing disc tissue overgrowth, particularly in the hinge region.

dsUAO71/ds05142 mutant wings display abnormal morphology. The region between wing veins L4 and L5 of dsUAO71/ds05142 mutant wings retains wild-type hair polarity, but has primarily anterior-posterior ridges.

dsUAO71/ds38k flies have abnormally shaped wings, but wing area is not much altered compared to controls. The flies have a planar polarity phenotype in the wing.

Flies expressing dsAct5C.T:Avic\GFP-EGFP in a dsUAO71/ds38k background show a reduction in wing area compared to wild type.

Flies expressing dsSAx3.Act5C.T:Avic\GFP-EGFP in a dsUAO71/ds38k background show a reduction in wing area compared to wild type.

ds38k/dsUAO71 wings show a planar cell polarity phenotype; reverse orientation and swirling of wing hairs is seen in the proximal region of the wing with high penetrance.

ds38k/dsUAO71 animals show defects in the directional preferences of growing microtubules in mutant wing cells compared to wild type; growing microtubules fail to converge along the proximodistal axis as strongly as in wild type. The distally biased asymmetry of microtubule growth that is seen in the wild-type is substantially weakened in the mutant at the anterior crossvein and at the L3-1 location (position of the most proximal dorsal campaniform sensillum on vein L3) at 24 hours after puparium formation (APF). In addition, plus end-proximal microtubules are more abundant at the L3-3 location (position of the most distal dorsal campaniform sensillum on vein L3) in the mutants at 24 hours APF (microtubule polarity is symmetrical at this position in wild-type flies at this stage).

dsUAO71 mutants exhibit largely depolarized denticle belts.

dsUAO71 larvae show mild wing disc overgrowth.

Postmitotic orientation is randomized in ds36D/dsUAO71 clones in the wing disc.

The orientation of cell division is randomized in ds36D/dsUAO71 mitotic clones in wing discs. Therefore, in contrast to wild-type postmitotic cells, ds36D/dsUAO71 postmitotic cells do not show any preferential orientation. Mitotic recombination clones in ds36D/dsUAO71 mutant wings show a rounded shape, instead of the elongated shape seen in wild-type clones. At the adult stage, ds36D/dsUAO71 wings are shorter, rounder and wider than wild type. Likewise, ds36D/dsUAO71 mitotic clones in eye discs show a defective shape and preferential orientations of cell divisions are lost. Resulting adult eyes are rounder than wild type. ds36D/dsUAO71 flies also have shorter, wider legs than wild-type flies.

Occasionally in ds38k/dsUAO71 escapers, the wing is replaced by a winglet and, posterior to it, a lateral protuberance that looks like an scutum and scutellum. The winglet is composed of proximal anterior structures arranged in a mirror-image duplication. The smallest examples of these winglets consist of a mirror image duplication of anterior hinge structures - the tegula and humeral sclerite. Larger examples also have a rudimentary wing blade consisting of a mirror image duplication of anterior structures - the costa and anterior wing margin.

ds38k/dsUAO71 flies have disorganised ommatidial polarity; no obvious equator is present. Flies expressing dsScer\UAS.P\T.cSa under the control of Scer\GAL4αTub84B.PL in a ds38k/dsUAO71 background have a normal overall pattern of polarisation in the eye; an equator can easily be discerned. 97.6% of ommatidia are correctly constructed and rotated in these animals. Expression of dsScer\UAS.P\T.cSa under the control of Scer\GAL4fj-VG1 in a ds38k/dsUAO71 background results in eyes with disorganised ommatidial polarity.

Planar polarity is disrupted in dsUAO71 eyes; dorsal and ventral ommatidia are intermixed and no obvious equatorial line can be drawn.

Homozygous clones in the eye can non-autonomously affect the planar polarity of wild-type ommatidia on the equatorial side of the clone. Polarity is also affected within the clone.

In the abdomen of dsUAO71 mutant flies, the anterior parts of the anterior compartments are fairly normal, but much of the rest of the anterior and posterior compartments is affected by whorls. In addition, hair orientation in the back half of the P compartments, both dorsal and ventral, is reversed In the tergites, dsUAO71 somatic clones are characterized by whorling hairs within the clone. In the anterior compartments clones cause some swirly repolarization of the hairs in front of the clone, but not behind. In the posterior compartments clones induce clear reversal of hairs behind the clone without affecting the front. Those clones situated at the compartment boundary reorient hairs outside the clone, both anterior to the clone and posterior to it. Hairs within the clone are more whorly than with fj expressing clones.

The wings of ds33k/dsUAO71 animals have a trichome swirling pattern that is distinct from that due to fz15/fz25. In ds33k/dsUAO71 wings, trichomes form on cell edges. The dorsal-ventral polarity of ommatidia in ds38k/dsUAO71 flies is partially randomised. Defects in anterior-posterior ommatidial polarity or randomised rotation are not seen.

About 40% of ommatidia in homozygous mutant clones show reversed polarity. When homozygous mutant clones are made specifically in the equatorial R3/R4 precursor, almost all (87%) of the consequent ommatidia are in the wild-type polarity. When clones are made specifically in the polar R3/R4 precursor, about half (43%) of the consequent ommatidia are in the reverse polarity. The mutant photoreceptor precursor eventually becomes an R3 photoreceptor 68% of the time (50% in wild-type). The eyes of homozygous mutant or dsUAO71/ds38k escapers have eyes with ommatidia that randomly adopt either dorsal or ventral polarity. Homozygous dsUAO71 mutant clones also display ommatidial polarity defects, both within the clone and in neighbouring wild-type tissue along the equatorial border of the clone.

Homozygous clones in the wing have a tissue polarity phenotype. Rare homozygous adult flies have shortened wings with misplaced and severely eroded crossveins, a short fat body and severely distorted legs. ds33k/dsUAO71 flies have reduced viability, with only approximately half the expected number of adults emerging. Most of the adults that emerge quickly get stuck in the food and die. These flies also have a severely distorted body shape. ds33k/dsUAO71 flies have a wing tissue polarity phenotype similar to that of dsUAO71 homozygotes and hemizygotes. There is a region at the anterior margin with a swirl of partially reversed polarity hairs. In the anterior/central proximal part of the wing blade there are regions of completely reversed polarity (hairs pointing proximally). These regions are separated from regions of relatively normal polarity by a region of swirling. The polarity disruptions are more severe on the ventral surface of the wing than the dorsal surface. Prehairs initiate in the vicinity of the distal vertex of the wing cells (as in wild-type) in wing regions of ds33k/dsUAO71 flies that do not show substantial polarity alterations. In most regions with abnormal polarity, prehairs initiate at an alternative location along the cell periphery. In the regions with reversed polarity, prehairs initiate at the proximal-most vertex, while in regions where hair polarity is close to random and in the regions at the centre of swirls, prehairs initiate in the central regions of the cells. Approximately half the wings of dsUAO71/ds1 flies have a very weak tissue polarity phenotype, consisting of a couple of multiple hair cells and/or a small region of polarity disruption in the proximal part of the wing.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Enhancer of
NOT Enhancer of
Statement
Reference

dsUAO71/dsYAC41 is a non-enhancer of visible | adult stage phenotype of inIH56

Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4αTub84B.PL, dsUAO71, ft15, ftUAS.cMa has trichome & tergite | cell non-autonomous | somatic clone phenotype, enhanceable | somatic clone by fjUAS.cZa/Scer\GAL4αTub84B.PL

dsUAO71 has denticle belt phenotype, enhanceable by ft15

dsUAO71 has denticle row 5 phenotype, enhanceable by ft15

dsUAO71 has denticle row 6 phenotype, enhanceable by ft15

ds1/dsUAO71 has wing phenotype, enhanceable by fjd1/fjd1

Suppressed by
Statement
Reference

Scer\GAL4αTub84B.PL, dsUAO71, dsecto.UAS, ft15 has trichome & tergite | cell non-autonomous | somatic clone phenotype, suppressible | somatic clone by fjUAS.cZa/Scer\GAL4αTub84B.PL

dsUAO71 has wing phenotype, suppressible by fzI.hs

dsUAO71 has wing hair phenotype, suppressible by fzI.hs

Enhancer of
Statement
Reference

dsUAO71/ds[+] is an enhancer of ommatidium phenotype of Scer\GAL4hs.2sev, dgoUAS.cFa

dsUAO71 is an enhancer of denticle belt phenotype of ft12/ft15

dsUAO71 is an enhancer of denticle row 5 phenotype of ft12/ft15

dsUAO71 is an enhancer of denticle row 6 phenotype of ft12/ft15

dsUAO71 is an enhancer of wing disc phenotype of ft8/ftG-rv

ds1/dsUAO71 is an enhancer of wing phenotype of fjd1

dsUAO71/ds[+] is an enhancer of crossvein phenotype of fjt14.Tag:Golgi(hGALNT3)

dsUAO71/ds[+] is an enhancer of wing phenotype of fjt14.Tag:Golgi(hGALNT3)

dsUAO71 is an enhancer of phenotype of fzunspecified

NOT Enhancer of
Statement
Reference

dsUAO71/ds[+] is a non-enhancer of wing hair phenotype of Scer\GAL4hs.2sev, dgoUAS.cFa

dsUAO71 is a non-enhancer of pigment cell | somatic clone phenotype of ft422

dsUAO71 is a non-enhancer of eye | somatic clone phenotype of ft422

Suppressor of
NOT Suppressor of
Statement
Reference

dsUAO71/ds[+] is a non-suppressor of wing hair phenotype of Scer\GAL4hs.2sev, dgoUAS.cFa

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

inIH56;dsYAC41/dsUAO71 double mutant adults display hair polarity defects in abdominal bristles that are similarly severe as in inIH56 single mutants.

Expression of wapVDRC.cUa substantially suppresses the wing disc morphological defects seen when dsΔECD.Scer\UAS is expressed under the control of Scer\GAL4hh.PU in a dsUAO71 mutant background.

The overgrowth of the wing disc caused by expression of ftΔICD.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hh-Gal4 is partially suppressed in a dsUAO71 background.

dsUAO71/+ enhances the ommatidial rotation defects induced by the overexpression of dgoScer\UAS.cFa under the control of Scer\GAL4hs.2sev.

dsUAO71 ft15 double mutant clones in the dorsal adult abdomen where the clones are also expressing dsecto.Scer\UAS under the control of Scer\GAL4αTub84B.PL strongly repolarise hairs behind the clone, reversing their polarity (100% of clones repolarise with a maximum range of up to seven cells). This repolarisation is largely suppressed by co-expression of fjScer\UAS.cZa within the clone (48% of clones show some repolarisation with a range of one to three cells).

dsUAO71 ft15 double mutant clones in the dorsal adult abdomen where the clones are also expressing ftScer\UAS.cMa under the control of Scer\GAL4αTub84B.PL cause repolarisation in front of the clones, so that hairs point away from the clone (64% of clones repolarise with a maximum range of seven cells). Co-expression of fjScer\UAS.cZa within these clones strengthens the repolarisation (95% of clones repolarise with a maximum range of seven cells).

Adding fjp1/fjd1 has little effect on the wing area of dsUAO71/ds38k flies.

Adding fjp1/fjd1 dramatically reduces the wing area of flies expressing dsAct5C.T:Avic\GFP-EGFP in a dsUAO71/ds38k background.

Adding fjp1/fjd1 has no effect on the wing area of flies expressing dsSAx3.Act5C.T:Avic\GFP-EGFP in a dsUAO71/ds38k background.

Adding fjp1/fjd1 has no effect on the wing area of flies expressing dsSDx3.Act5C.T:Avic\GFP-EGFP in a dsUAO71/ds38k background.

dsUAO71 ft15 double mutants exhibit largely depolarized denticle belts (with rows 5 and 6 more disturbed than in dsUAO71 mutants). In addition to the orientation defects, the rows partially lose their distinctive characteristics.

Expression of fzScer\UAS.cZa in dsUAO71 larvae under the control of Scer\GAL4ptc-559.1 does not affect denticle belt polarity.

dsUAO71, ftG-rv/ft8 double mutant larvae show an enhancement of the wing disc overgrowth phenotype of ftG-rv/ft8 single mutants.

dsUAO71 mutants do not significantly enhance the extra ommatidial cell phenotype of ft422 mutants.

ds38k fjN7/dsUAO71 fjd1 flies have disorganised ommatidial polarity; no obvious equator is present. Flies expressing both fjScer\UAS.cZa and dsScer\UAS.P\T.cSa under the control of Scer\GAL4αTub84B.PL in a ds38k fjN7/dsUAO71 fjd1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing dsScer\UAS.P\T.cSa under the control of Scer\GAL4αTub84B.PL in a ds38k fjN7/dsUAO71 fjd1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing fjScer\UAS.cZa under the control of Scer\GAL4αTub84B.PL in a ds38k fjN7/dsUAO71 fjd1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing dsScer\UAS.P\T.cSa under the control of Scer\GAL4fj-VG1 in a dsUAO71 fjVG1/ds38k fjN7 background have a disorganised ommatidial polarity; the eye contains a clearly perceptible equator in which most of the nearby ommatidia are reversed in polarity and thus point away from the equator. Flies expressing both fjScer\UAS.cZa and dsScer\UAS.P\T.cSa under the control of Scer\GAL4αTub84B.PL in a ds38k fjN7/dsUAO71 fjd1 background have normal polarity throughout the wing blade with the exception of a small region along the anterior proximal margin of the wing, where a small swirl is often present. Flies expressing dsScer\UAS.P\T.cSa under the control of Scer\GAL4αTub84B.PL in a ds38k fjN7/dsUAO71 fjd1 background have normal polarity throughout the wing blade with the exception of a small region along the anterior proximal margin of the wing, where a small swirl is often present.

Unlike either single mutant, 'fjd1 ds1/fjd1 dsUAO71' flies completely lack a posterior crossvein and have defects in wing hair polarity. 16% of ommatidia in these double mutants are inverted compared to less than 1% in either single mutant. The number of ommatidial inversions in ds1/dsUAO71 flies is also enhanced by fjunspecified/+. Reduction in the distance between anterior and posterior cross-veins in fjt14.T:Hsap\GALNT3 flies is enhanced by dsUAO71/+.

When ftG-rv/dsUAO71 homozygous mutant clones are made specifically in the equatorial R3/R4 precursor, most (64%) of the consequent ommatidia are in the reverse polarity. When clones are made specifically in the polar R3/R4 precursor, almost all (97.5%) of the consequent ommatidia are in the wild-type polarity. The mutant photoreceptor precursor eventually becomes an R4 photoreceptor 80% of the time (50% in wild-type). When dsUAO71 homozygous mutant clones are made specifically in the equatorial R3/R4 precursors of fjN7 mutant flies, almost all (92%) of the consequent ommatidia are in the wild-type polarity. When clones are made specifically in the polar R3/R4 precursor, most (73%) of the consequent ommatidia are in the reverse polarity. The mutant photoreceptor precursor eventually becomes an R3 photoreceptor 85% of the time (50% in wild-type). When fjN7 homozygous mutant clones are made in the R3/R4 precursors of dsUAO71 mutant flies, The mutant photoreceptor precursor eventually becomes an R3 photoreceptor half of the time (50% in wild-type). When fjN7 homozygous mutant clones are made in the R3/R4 precursors of dsUAO71 mutant flies, The mutant photoreceptor precursor eventually becomes an R3 photoreceptor half of the time (50% in wild-type).

The distinctive ds polarity phenotype of ds33k/dsUAO71 flies (regions of reversed polarity on the wing) is suppressed by expression of fzI.hs 24 hours after white prepupa formation using a 1 hour heat shock. The appendage shape abnormalities and wing vein placement abnormalities are not suppressed. The domineering nonautonomy of fz1 clones is enhanced by dsUAO71/ds33k.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

The planar polarity defects seen in the wings of dsUAO71/ds38k animals are almost completely rescued by dsAct5C.T:Avic\GFP-EGFP.

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Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Separable from: TBCD1

The DL1 olfactory projection neuron (PN) defects observed on the dsUAO71 chromosome are caused by a second-site mutation, TBCD1 : a ds rescue construct is unable to rescue the construct, the PN phenotypes and ds mutation can be genetically segregated using meiotic recombination, and another strong ds mutant does not show any defects in PN morphology.

Comments
Comments

Analysis of ds33k/dsUAO71 clones shows that ds displays domineering cell nonautonomy.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (37)