Allele Dmel\cora⁵

General Information
Symbol	Dmel\cora⁵	Species	D. melanogaster
Name		FlyBase ID	FBal0089377
Feature type	allele	Associated gene	Dmel\cora
Also Known As	cor⁵

Allele class	loss of function allele, amorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Bauer et al., 2004) loss of function allele (Ward et al., 1998)
Mutagen	ethyl methanesulfonate (Lamb et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	cell polarity defective (with cora¹⁵) (Venema et al., 2004) lethal \| embryonic stage \| recessive (Lamb et al., 1998) lethal \| recessive (Ward et al., 2001) neuroanatomy defective (Chen et al., 2005) neuroanatomy defective (with cora^k08713) (Chen et al., 2005) neurophysiology defective (Chen et al., 2005) neurophysiology defective (with cora^k08713) (Chen et al., 2005)
Phenotype Manifest In
	denticle belt (Lamb et al., 1998) dorsal closure embryo (Ward et al., 2001) dorsal trunk primordium (with cora⁴) (Paul et al., 2003) embryonic/first instar larval cuticle (Ward et al., 2001, Lamb et al., 1998) embryonic/first instar larval cuticle \| dorsal (Lamb et al., 1998, Ward et al., 1998) embryonic/larval salivary gland \| embryonic stage (Lamb et al., 1998, Ward et al., 1998) embryonic ganglionic branch & embryonic tracheole (with cora⁴) (Paul et al., 2003) pleated septate junction (Ward et al., 2001, Lamb et al., 1998) presumptive embryonic/larval tracheal system (Ward et al., 2001, Llimargas et al., 2004) presumptive embryonic salivary gland (Ward et al., 2001) septate junction (Genova and Fehon, 2003) septate junction \| embryonic stage (Bauer et al., 2004) wing hair (with cora¹⁵) (Venema et al., 2004)
Detailed Description
	Statement Reference Homozygous cora⁵ mutants and cora^k08713/cora⁵ transheterozygotes show electrophysiologically detectable loss of glutamate receptors. (Chen et al., 2005) The septate junctions of cora⁵ mutant stage 15 embryos are disrupted. (Bauer et al., 2004) The tracheal systems of lates stage cora⁵ homozygous embryos have overgrown tubes with unusual expansions, defects in the accumulation of lumen antigens and lumen breaks. (Llimargas et al., 2004) cora⁵/cora¹⁵ mutant wings exhibit a wing hair chevron phenotype and matches of non-parallel alignment. (Venema et al., 2004) In cora⁵ homozygous mutants the adherens junction remains intact, with the regular spacing of plasma membranes maintained. However, the septae normally located between these membranes are reduced in number or absent. cora⁵/cora⁵ somatic clones generated in larvae (approx. 72 hours after egg laying) do not survive in adult tissues. (Genova and Fehon, 2003) In cora⁵/cora⁴ embryos, tracheal phenotypes are apparent from stage 15. At stage 16 in these embryos the average dorsal trunk length is significantly greater than wild type (P<0.005) (122+/-2% mean+/-s.e.m., n>5, normalized to stage 16 wild-type value). In addition these embryos have moderately severe diameter increases in the dorsal trunk and other primary tracheal branches, and some ganglionic branches exhibit missing lumen. Diameter increases are also seen in the primary tracheal branches of cora⁵ homozygous embryos. Unlike wild-type, post stage 15 trachea in cora⁵ homozygotes are unable to exclude from their lumens, a fluorescently labelled 10kDa dextran injected into the body cavity. This is consistent with these trachea lacking a functioning septate junction barrier. (Paul et al., 2003) Homozygous embryos have a dorsal open phenotype, cuticular thinning, necrosis of the salivary glands and a tracheal inflation defect. Embryos lack the individual septae that normally characterise the pleated septate junction. The salivary gland epithelium shows permeability to rhodamine-dextran, in contrast to wild type. (Ward et al., 2001) Homozygous embryos have a faint cuticle and a dorsal hole. The remains of the salivary glands are present as necrotic material. The epicuticle fails to adhere to the procuticle. The pleated septae that normally characterise the pleated septate junction are absent in mutant epithelia, and the transepithelial barrier function of the septate junction is also disrupted. The adherens junction appears unaffected in mutant embryos. No adult escapers are produced. Hemizygous embryos have a dorsal hole and detached cuticle. The denticle belts contain fewer denticles than normal, although the overall pattern of the denticle belts is normal. (Lamb et al., 1998) Homozygous embryos have dorsal closure defects; dorsal holes are seen in the cuticle. Necrosis is seen in the salivary glands. (Ward et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference cora⁵ is a suppressor of visible \| dominant phenotype of Egfr^E1 (Lamb et al., 1998)
Phenotype Manifest In
NOT Enhanced by
Statement Reference cora⁵ has presumptive embryonic/larval tracheal system phenotype, non-enhanceable by nrv2^nwu3/nrv2^nwu3 (Paul et al., 2003)
NOT suppressed by
Statement Reference cora⁵ has presumptive embryonic/larval tracheal system phenotype, non-suppressible by nrv2^nwu3/nrv2^nwu3 (Paul et al., 2003)
NOT Enhancer of
Statement Reference cora⁵/cora⁵ is a non-enhancer of presumptive embryonic/larval tracheal system phenotype of nrv2^nwu3 (Paul et al., 2003, Paul et al., 2003)
Suppressor of
Statement Reference cora⁵ is a suppressor of eye phenotype of Egfr^E1 (Lamb et al., 1998)
NOT Suppressor of
Statement Reference cora⁵/cora⁵ is a non-suppressor of presumptive embryonic/larval tracheal system phenotype of nrv2^nwu3 (Paul et al., 2003, Paul et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference nrv2^nwu3; cora⁵ double mutant embryos have the same phenotype as either single mutant. (Paul et al., 2003) Dominantly suppresses the rough eye phenotype of Egfr^E1/+ heterozygotes; the eye is smaller than wild type with some roughening. (Lamb et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	cora¹ (Lamb et al., 1998) cora² (Lamb et al., 1998)
Rescued by	cora⁵ is rescued by cora^1.Ubi-p63E (Lamb et al., 1998, Ward et al., 2001) cora⁵ is rescued by cora^2.Ubi-p63E (Ward et al., 2001) cora⁵ is rescued by cora^3.Ubi-p63E (Ward et al., 2001) cora⁵ is rescued by cora^{fl.hs.T:Hsap\MYC} (Ward et al., 2001)
Partially rescued by	cora⁵ is partially rescued by cora^{1-383.hs.T:Hsap\MYC} (Ward et al., 2001)
Not rescued by	cora⁵ is not rescued by cora^{1-383.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^T80 (Ward et al., 2001) cora⁵ is not rescued by cora^{1-1698.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^T80 (Ward et al., 2001) cora⁵ is not rescued by cora^{378-1698.hs.T:Hsap\MYC} (Ward et al., 2001)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	cor⁵ (Genova and Fehon, 2003, Behr et al., 2003, Wu et al., 2004, Ward et al., 2001, Lamb et al., 1998, Ward et al., 1998, Wu et al., 2007, Nelson et al., 2010) cora⁵ (Chen et al., 2005, Izumi et al., 2012) coracle⁵ (Stork et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 15 )
Research paper	Izumi et al., 2012, J. Cell Sci. 125(20): 4923--4933 A novel protein complex, Mesh-Ssk, is required for septate junction formation in the Drosophila midgut. [FBrf0220284] Nelson et al., 2010, Genetics 185(3): 831--839 The Drosophila Claudin Kune-kune Is Required for Septate Junction Organization and Tracheal Tube Size Control. [FBrf0211332] Stork et al., 2008, J. Neurosci. 28(3): 587--597 Organization and function of the blood-brain barrier in Drosophila. [FBrf0202685] Wu et al., 2007, Development 134(5): 999--1009 Drosophila Varicose, a member of a new subgroup of basolateral MAGUKs, is required for septate junctions and tracheal morphogenesis. [FBrf0194079] Chen et al., 2005, J. Neurosci. 25(28): 6667--6675 The 4.1 protein coracle mediates subunit-selective anchoring of Drosophila glutamate receptors to the postsynaptic actin cytoskeleton. [FBrf0187898] Bauer et al., 2004, Mol. Biol. Cell 15(6): 2992--3004 Gap junction channel protein innexin 2 is essential for epithelial morphogenesis in the Drosophila embryo. [FBrf0179129] Llimargas et al., 2004, Development 131(1): 181--190 Lachesin is a component of a septate junction-based mechanism that controls tube size and epithelial integrity in the Drosophila tracheal system. [FBrf0167526] Venema et al., 2004, Dev. Biol. 275(2): 301--314 Transient apical polarization of Gliotactin and Coracle is required for parallel alignment of wing hairs in Drosophila. [FBrf0180106] Wu et al., 2004, J. Cell Biol. 164(2): 313--323 Sinuous is a Drosophila claudin required for septate junction organization and epithelial tube size control. [FBrf0167773] Behr et al., 2003, Dev. Cell 5(4): 611--620 The claudin-like megatrachea is essential in septate junctions for the epithelial barrier function in Drosophila. [FBrf0167473] Genova and Fehon, 2003, J. Cell Biol. 161(5): 979--989 Neuroglian, Gliotactin, and the Na+/K+ ATPase are essential for septate junction function in Drosophila. [FBrf0160546] Paul et al., 2003, Development 130(20): 4963--4974 The Na+/K+ ATPase is required for septate junction function and epithelial tube-size control in the Drosophila tracheal system. [FBrf0162083] Ward et al., 2001, Genetics 159(1): 219--228 The Protein 4.1, Ezrin, Radixin, Moesin (FERM) Domain of Drosophila Coracle, a Cytoplasmic Component of the Septate Junction, Provides Functions Essential for Embryonic Development and Imaginal Cell Proliferation. [FBrf0138412] Lamb et al., 1998, Mol. Biol. Cell 9(12): 3505--3519 Drosophila coracle, a member of the protein 4.1 superfamily, has essential structural functions in the septate junctions and developmental functions in embryonic and adult epithelial cells. [FBrf0105869] Ward et al., 1998, J. Cell Biol. 140(6): 1463--1473 A conserved functional domain of Drosophila coracle is required for localization at the septate junction and has membrane-organizing activity. [FBrf0102020]

Allele Dmel\cora5

Allele Dmel\cora⁵