|Feature type||allele||Associated gene||Dmel\cnn|
|Map ( GBrowse )|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Nucleotide substitution: C2875T. Amino acid replacement: Q959@. Mutation results in a 190 amino acid truncation of the protein. The mutation falls within the third leucine zipper.
Stop codon upstream of the normal translation termination signal.
|Phenotype Manifest In|
Only 37% of testis somatic cyst stem cells show repositioning of the mitotic spindle during anaphase in cnn[mfs3]/cnn[HK21] males compared to 78% of cases in heterozygous controls.
The failure of embryogenesis in mutant animals is associated with the appearance of multiple metaphase spindles fused at a common pole.
The number of germ cells associated with the hub is increased 20-30% in homozygous males, from an average of 8.94 GSCs per hub in wild type to 10.69 GSCs per hub in cnnHK21/cnnmfs3 males. In some cases in cnnHK21/cnnmfs3 testes, a GSC divides and the two daughter cells remain in contact with the hub (this is not observed in the wild type).
Homozygous females produce embryos that arrest prior to cellularisation. Embryos derived from cnnmfs3/Df(2R)cnn females have severe defects in nuclear division and distribution. The embryos never achieve cellularisation.
Cytokinesis and karyokinesis during meiotic divisions are disrupted in males. The percentage of aberrant spermatids varies from testis to testis (20-50%), and the cytokinetic defects are more severe than karyokinetic defects.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Fails to complement|
|Partially rescued by|
Expression of cnn[PA.Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] rescues embryogenesis in cnn[mfs3] animals; approximately 92% of eggs produced by cnn[mfs3] females expressing cnn[PA.Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] hatch.
|Stocks ( 1 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
|Secondary FlyBase IDs|
|References ( 5 )|