Allele Dmel\cnn^mfs3

General Information
Symbol	Dmel\cnn^mfs3	Species	D. melanogaster
Name		FlyBase ID	FBal0089468
Feature type	allele	Associated gene	Dmel\cnn

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Li et al., 1998, Megraw et al., 1999)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:9,328,212..9,328,212 evidence=experimental na_change=C9328212T pr_change=Q901\|cnn-PD,Q1131\|cnn-PC,Q931\|cnn-PB,Q959\| cnn-PA,Q941@\|cnn-PE reported_na_change=C2875T. reported_pr_change=Q959@ (Megraw et al., 1999)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: C2875T. Amino acid replacement: Q959@. Mutation results in a 190 amino acid truncation of the protein. The mutation falls within the third leucine zipper. (Megraw et al., 1999) Stop codon upstream of the normal translation termination signal. (Li et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	cytokinesis defective \| male (with Df(2R)cnn) (Li et al., 1998) female sterile (Li et al., 1998) female sterile \| recessive (Megraw et al., 1999) lethal \| embryonic stage (Eisman et al., 2009) lethal \| embryonic stage \| maternal effect \| recessive (Megraw et al., 1999) male sterile (Li et al., 1998) male sterile \| recessive (Megraw et al., 1999) mitotic cell cycle defective (Eisman et al., 2009) viable (Megraw et al., 1999)
Phenotype Manifest In
	cyst progenitor cell (with cnn^HK21) (Cheng et al., 2011) embryo \| maternal effect (Megraw et al., 1999) male germline stem cell \| supernumerary (with cnn^HK21) (Yamashita et al., 2003) meiotic cell cycle \| male (Li et al., 1998) nucleus \| embryonic stage \| maternal effect (Megraw et al., 1999) spermatid (Li et al., 1998) spindle (Eisman et al., 2009) spindle (with cnn^HK21) (Cheng et al., 2011)
Detailed Description
	Statement Reference Only 37% of testis somatic cyst stem cells show repositioning of the mitotic spindle during anaphase in cnn[mfs3]/cnn[HK21] males compared to 78% of cases in heterozygous controls. (Cheng et al., 2011) The failure of embryogenesis in mutant animals is associated with the appearance of multiple metaphase spindles fused at a common pole. (Eisman et al., 2009) The number of germ cells associated with the hub is increased 20-30% in homozygous males, from an average of 8.94 GSCs per hub in wild type to 10.69 GSCs per hub in cnn^HK21/cnn^mfs3 males. In some cases in cnn^HK21/cnn^mfs3 testes, a GSC divides and the two daughter cells remain in contact with the hub (this is not observed in the wild type). (Yamashita et al., 2003) Homozygous females produce embryos that arrest prior to cellularisation. Embryos derived from cnn^mfs3/Df(2R)cnn females have severe defects in nuclear division and distribution. The embryos never achieve cellularisation. (Megraw et al., 1999) Cytokinesis and karyokinesis during meiotic divisions are disrupted in males. The percentage of aberrant spermatids varies from testis to testis (20-50%), and the cytokinetic defects are more severe than karyokinetic defects. (Li et al., 1998)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	cnn^HK21 (Megraw et al., 1999)
Rescued by	cnn^mfs3 is rescued by cnn^+t16 (Li et al., 1998, Megraw et al., 1999) cnn^mfs3 is rescued by Scer\GAL4^{nos.UTR.T:Hsim\VP16}/cnn^{PA.Scer\UAS.P\T.T:Avic\GFP} (Eisman et al., 2009)
Partially rescued by	cnn^mfs3 is partially rescued by cnn^hs.PL (Li et al., 1998)
Comments	Expression of cnn[PA.Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] rescues embryogenesis in cnn[mfs3] animals; approximately 92% of eggs produced by cnn[mfs3] females expressing cnn[PA.Scer\UAS.P\T.T:Avic\GFP] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] hatch. (Eisman et al., 2009) Male and female sterility is rescued by cnn^+t16. Male sterility is rescued by basal levels of expression (at 25^oC) of cnn^hs.PL. (Li et al., 1998)
Stocks ( 1 )
Bloomington	27626 w¹¹¹⁸; cnn^mfs3/SM5
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	arr^mfs3 cnn^mfs3 (Eisman et al., 2009, Cheng et al., 2011) mfs3 (Li et al., 1998)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Cheng et al., 2011, Development 138(5): 831--837 Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning. [FBrf0213009] Eisman et al., 2009, Genetics 182(4): 979--997 Centrosomin: A Complex Mix of Long and Short Isoforms Is Required for Centrosome Function During Early Development in Drosophila melanogaster. [FBrf0208637] Yamashita et al., 2003, Science 301(5639): 1547--1550 Orientation of asymmetric stem cell division by the APC tumor suppressor and centrosome. [FBrf0161562] Megraw et al., 1999, Development 126(13): 2829--2839 The Centrosomin protein is required for centrosome assembly and function during cleavage in Drosophila. [FBrf0108930] Li et al., 1998, J. Cell Biol. 141(2): 455--467 Drosophila centrosomin protein is required for male meiosis and assembly of the flagellar axoneme. [FBrf0102357]

Allele Dmel\cnnmfs3

Allele Dmel\cnn^mfs3