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General Information
Symbol
Dmel\Klc8ex94
Species
D. melanogaster
Name
FlyBase ID
FBal0089617
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Df(3L)8ex94
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Imprecise excision of a P-element, deleting most of the Klc transcription unit.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Klc8ex94/+ flies do not exhibit significantly increased bang sensitivity, nor any significant difference in seizure threshold or spikes per discharge in an electroconvulsive stimulation paradigm, as compared to wild type.

    Klc8ex94/Klcc02312 transheterozygotes show a partial disruption of the internal structure of the third instar larval mushroom body, as compared to controls.

    Klc8ex94 homozygous clones within mosaic third instar larval mushroom bodies show prevalent peduncle defects (split fascicles are observed in the majority of cases) and fully prevalent defects in the extension of calix dendrites, as compared to control clones.

    Homozygous null Klc8ex94 eye clones in third instar larvae and adults are somewhat small and roughened, with axons that are frequently disordered and sometimes short and defasciculated, despite photoreceptors maintaining viability and undergoing normal differentiation. Axonal mitochondria are numerous, as in controls.

    Ooplasmic streaming still occurs in mutant stage 9 and stage 10b oocytes, although the movements are often less vigorous than in wild type.

    Homozygotes exhibit organelle accumulation in larval neurons. Heterozygotes do not show an organelle jamming phenotype.

    Homozygotes die at the boundary of the second and third larval instar stages. Klc8ex94/Klc59A animals survive until the late third larval instar stage. Klc8ex94/Klc59A larvae flip their posterior end off the surface of the food during crawling.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Enhanced by
    NOT Enhanced by
    Enhancer of
    Statement
    Reference
    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Klc8ex94/+ fully suppresses the bang sensitivity and lowered seizure threshold phenotypes of pksple-1/+ mutants.

    Atg125, Klc8ex94 heterozygous third instar larval mushroom bodies show defects in the extension of calix dendrites, but no obvious core integrity, as compared to either heterozygous controls.

    Heterozygosity for Klc8ex94 dramatically increases the percentage of eggs laid by heat shocked Dmnhs.PD flies with displaces and/or highly reduced dorsal appendages.

    The addition of ApplScer\UAS.cTa to Klc8ex94/+ animals causes the organelle jamming phenotype to be seen in this animals, thus enhancing the phenotype. The addition of ApplsdΔC.Scer\UAS or Appls.Scer\UAS has no effect on this phenotype.

    Xenogenetic Interactions
    Statement
    Reference

    Klc8ex94/+ enhances the partialy lethality of individuals expressing either Hsap\MAPTScer\UAS.cWa or Hsap\MAPTR406W.Scer\UAS under the control of Scer\GAL4Appl.G1a.

    The addition of Hsap\APP695-Swedish.Scer\UAS, Hsap\APP695SPA4CT.Scer\UAS.T:Hsap\MYC, Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC, or Hsap\APP695.T.Scer\UAS (when driven by Scer\GAL4Appl.G1a) to Klc8ex94/+ animals causes the organelle jamming phenotype to be seen in this animals, thus enhancing the phenotype. The addition of Hsap\APP695ΔCT.Scer\UAS.T:Hsap\MYC has no effect on this phenotype.

    Complementation and Rescue Data
    Partially rescued by
    Comments

    KlcGEN rescues homozygous and Klc8ex94/Klc59A animals to adulthood. KlcUbi-p63E.T:Hsap\MYC rescues Klc8ex94/Klc59A animals to adulthood. KlcUbi-p63E.T:Hsap\MYC allows homozygotes to survive to the late third larval instar stage, although these animals never enter the wandering stage that occurs before pupariation. Klc8ex94/Klc59A larvae flip their posterior end off the surface of the food during crawling. This phenotype is rescued by KlcGEN and KlcUbi-p63E.T:Hsap\MYC.

    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    Comments
    Comments

    Complements lethal mutations in Ptp69D.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (5)
    References (19)