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General Information
Symbol
Hsap\CASP1UAS.cSa
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Shigenaga
FlyBase ID
FBal0089638
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Ice
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a 1.3kb Hsap\CASP1 cDNA that contains the entire protein-coding region.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4Hml.PG-, Scer\GAL4Pxn.PS- or Scer\GAL4srp.D.cCa-mediated expression of Hsap\CASP1Scer\UAS.cSa ablates larval hemocytes.

Scer\GAL4srp.D.cCa-mediated expression of Hsap\CASP1Scer\UAS.cSa leads to a high lethality rate during early larval development - these larvae are devoid of hemocytes and rarely reach the third instar larval stage.

The paired lobes of the larval haematopoietic organ (lymph gland) are reduced in size in Scer\GAL4Pxn.PS, Hsap\CASP1Scer\UAS.cSa or Scer\GAL4Hml.PG, Hsap\CASP1Scer\UAS.cSa animals. These animals die during pupal stages, but the duration of the three larval stages is not altered and no melanotic tumours are observed.

Unlike wild type, Scer\GAL4Hml.PG Hsap\CASP1Scer\UAS.cSa larvae fail to clear bacterial loads after septic injury (with E. coli, E. carotovara or M. luteus) or oral infection (with E. carotovara).

Scer\GAL4sca-537.4-mediated expression causes lethality at different stages from embryos to pupae and expression of some lines allows viability (Cele\ced-3 is expressed in these lines). Mediated expression can elicit cell death in Drosophila.

Scer\GAL4389-mediated expression causes wing defects in emerging flies: anterior and posterior regions of the wing are missing. Cell death is induced in two lateral regions of the third instar wing disc. Scer\GAL411H-1-mediated expression causes larval lethality or in some lines 'giant larvae' that remain alive for as long as 3 weeks. Most of the cells that secrete ecdysone are missing in the prothoracic gland of these larvae. Cell death is also observed in the ring gland of first instar larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Cell death can be blocked by coexpressing BacA\p35Scer\UAS.cHa, using Scer\GAL4sca-537.4.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\CASP1Scer\UAS.cSa
Hsap\CASP1UAS.cSa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Shigenaga
Secondary FlyBase IDs
    References (4)