FlyBase Allele Report: Hsap\CASP1[UAS.cSa]

General Information

Symbol

Hsap\CASP1^UAS.cSa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Shigenaga

FlyBase ID

FBal0089638

Feature type

allele

Associated gene

Hsap\CASP1

Associated Insertion(s)

Carried in Construct

P{UAS-Hsap\CASP1.S}

Also Known As

UAS-Ice

Key Links

Transgenic product class

cDNA

(Shigenaga et al., 1997)

wild_type

(Shigenaga et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Shigenaga et al., 1997)

wild_type

(Shigenaga et al., 1997)

Mutagen

in vitro construct

(Shigenaga et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-Hsap\CASP1.S}

Cytology

Description

UASt regulatory sequences drive expression of a 1.3kb Hsap\CASP1 cDNA that contains the entire protein-coding region.

(Shigenaga et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\CASP1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^Hml.PG

(Shia et al., 2009)

increased cell death, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

lethal - all die before end of larval stage, with Scer\GAL4^11H-1

(Shigenaga et al., 1997)

lethal - all die before end of pupal stage, with Scer\GAL4³⁸⁹

(Shigenaga et al., 1997)

lethal - all die before end of pupal stage, with Scer\GAL4^ptc-559.1

(Shigenaga et al., 1997)

lethal - all die during larval stage, with Scer\GAL4^srp.D.cCa

(Shia et al., 2009)

lethal - all die during pupal stage, with Scer\GAL4^Hml.PG

(Shia et al., 2009)

lethal - all die during pupal stage, with Scer\GAL4^Pxn.PS

(Shia et al., 2009)

partially lethal - majority die, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

some die during larval stage, with Scer\GAL4^11H-1

(Shigenaga et al., 1997)

some die during pupal stage, with Scer\GAL4³⁸⁹

(Shigenaga et al., 1997)

some die during pupal stage, with Scer\GAL4^ptc-559.1

(Shigenaga et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^Hml.PG-, Scer\GAL4^Pxn.PS- or Scer\GAL4^srp.D.cCa-mediated expression of Hsap\CASP1^Scer\UAS.cSa ablates larval hemocytes.

Scer\GAL4^srp.D.cCa-mediated expression of Hsap\CASP1^Scer\UAS.cSa leads to a high lethality rate during early larval development - these larvae are devoid of hemocytes and rarely reach the third instar larval stage.

The paired lobes of the larval haematopoietic organ (lymph gland) are reduced in size in Scer\GAL4^Pxn.PS, Hsap\CASP1^Scer\UAS.cSa or Scer\GAL4^Hml.PG, Hsap\CASP1^Scer\UAS.cSa animals. These animals die during pupal stages, but the duration of the three larval stages is not altered and no melanotic tumours are observed.

Unlike wild type, Scer\GAL4^Hml.PG Hsap\CASP1^Scer\UAS.cSa larvae fail to clear bacterial loads after septic injury (with E. coli, E. carotovara or M. luteus) or oral infection (with E. carotovara).

(Shia et al., 2009)

Scer\GAL4^sca-537.4-mediated expression causes lethality at different stages from embryos to pupae and expression of some lines allows viability (Cele\ced-3 is expressed in these lines). Mediated expression can elicit cell death in Drosophila.

(Shigenaga et al., 1997)

Scer\GAL4³⁸⁹-mediated expression causes wing defects in emerging flies: anterior and posterior regions of the wing are missing. Cell death is induced in two lateral regions of the third instar wing disc. Scer\GAL4^11H-1-mediated expression causes larval lethality or in some lines 'giant larvae' that remain alive for as long as 3 weeks. Most of the cells that secrete ecdysone are missing in the prothoracic gland of these larvae. Cell death is also observed in the ring gland of first instar larvae.

(Shigenaga et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\CASP1^UAS.cSa, Scer\GAL4^sca-537.4 has partially lethal - majority die phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\CASP1^UAS.cSa, Scer\GAL4^sca-537.4 has macrochaeta | pharate adult stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Cell death can be blocked by coexpressing BacA\p35^Scer\UAS.cHa, using Scer\GAL4^sca-537.4.

(Shigenaga et al., 1997)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\CASP1^Scer\UAS.cSa

Hsap\CASP1^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Shigenaga

(Shigenaga et al., 1997)

Secondary FlyBase IDs

References (4)

Report Sections

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