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General Information
Symbol
Cele\ced-3UAS.cSa
Species
C. elegans
Name
Saccharomyces cerevisiae UAS construct a of Shigenaga
FlyBase ID
FBal0089771
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ced3
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a 2.5kb Cele\ced-3 cDNA that contains entire protein-coding region.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4Hml.PG-, Scer\GAL4Pxn.PS- or Scer\GAL4srp.D.cCa-mediated expression of Cele\ced-3Scer\UAS.cSa ablates larval hemocytes.

Scer\GAL4srp.D.cCa-mediated expression of Cele\ced-3Scer\UAS.cSa leads to a high lethality rate during early larval development - these larvae are devoid of hemocytes and rarely reach the third instar larval stage.

When Cele\ced-3Scer\UAS.cSa is driven by Scer\GAL4rQ286.11, to induce ablation of glial cells, a spectrum of nervous system phenotypes are seen in embryonic hemisegments, which range from having a disrupted CNS and PNS pathways to being essentially normal. Embryos that lack a visible glial sheath show defasciculated peripheral nerves as well as axonal pathfinding defects.

Scer\GAL4sca-537.4-mediated expression causes lethality at different stages from embryos to pupae and expression of some lines allows viability (Cele\ced-3 is expressed in these lines). Mediated expression can elicit cell death in embryos, as shown by TUNEL. Evidence of ectopic cell death can be seen morphologically: irregular pattern of the peripheral nervous system. Cell death induced in sensory cell anlagen results in a severe defect in the development of sensory bristles: almost all bristles on the notum, head, abdomen and compound eyes of late pupae are missing. Ectopic cell death is not observed in the eye disc, the external morphology and number of ommatidia of the pupal eyes is not affected.

Scer\GAL4ptc-559.1-mediated expression causes deletion of the scutellum. Wings are not well developed and some of the veins are missing. Arista are missing and the shape of the compound eye is abnormal. Legs are also deleted. Defects are caused by ectopic cell death in the wing disc, lateral regions of the eye disc and in the leg disc. Scer\GAL4389-mediated expression causes wing defects in emerging flies: anterior and posterior regions of the wing are missing. Cell death is induced in two lateral regions of the third instar wing disc. Scer\GAL411H-1-mediated expression causes larval lethality or in some lines 'giant larvae' that remain alive for as long as 3 weeks. Most of the cells that secrete ecdysone are missing in the prothoracic gland of these larvae. Cell death is also observed in the ring gland of first instar larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Cell death can be blocked by coexpressing BacA\p35Scer\UAS.cHa, using Scer\GAL4sca-537.4.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Cele\ced-3Scer\UAS.cSa
Cele\ced-3UAS.cSa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Shigenaga
Secondary FlyBase IDs
    References (6)