UASt regulatory sequences drive expression of a 2.5kb Cele\ced-3 cDNA that contains entire protein-coding region.
macrochaeta & abdomen & pupa, with Scer\GAL4sca-537.4
macrochaeta & head & pupa, with Scer\GAL4sca-537.4
macrochaeta & scutum & pupa, with Scer\GAL4sca-537.4
Scer\GAL4Hml.PG-, Scer\GAL4Pxn.PS- or Scer\GAL4srp.D.cCa-mediated expression of Cele\ced-3Scer\UAS.cSa ablates larval hemocytes.
Scer\GAL4srp.D.cCa-mediated expression of Cele\ced-3Scer\UAS.cSa leads to a high lethality rate during early larval development - these larvae are devoid of hemocytes and rarely reach the third instar larval stage.
When Cele\ced-3Scer\UAS.cSa is driven by Scer\GAL4rQ286.11, to induce ablation of glial cells, a spectrum of nervous system phenotypes are seen in embryonic hemisegments, which range from having a disrupted CNS and PNS pathways to being essentially normal. Embryos that lack a visible glial sheath show defasciculated peripheral nerves as well as axonal pathfinding defects.
Scer\GAL4sca-537.4-mediated expression causes lethality at different stages from embryos to pupae and expression of some lines allows viability (Cele\ced-3 is expressed in these lines). Mediated expression can elicit cell death in embryos, as shown by TUNEL. Evidence of ectopic cell death can be seen morphologically: irregular pattern of the peripheral nervous system. Cell death induced in sensory cell anlagen results in a severe defect in the development of sensory bristles: almost all bristles on the notum, head, abdomen and compound eyes of late pupae are missing. Ectopic cell death is not observed in the eye disc, the external morphology and number of ommatidia of the pupal eyes is not affected.
Scer\GAL4ptc-559.1-mediated expression causes deletion of the scutellum. Wings are not well developed and some of the veins are missing. Arista are missing and the shape of the compound eye is abnormal. Legs are also deleted. Defects are caused by ectopic cell death in the wing disc, lateral regions of the eye disc and in the leg disc. Scer\GAL4389-mediated expression causes wing defects in emerging flies: anterior and posterior regions of the wing are missing. Cell death is induced in two lateral regions of the third instar wing disc. Scer\GAL411H-1-mediated expression causes larval lethality or in some lines 'giant larvae' that remain alive for as long as 3 weeks. Most of the cells that secrete ecdysone are missing in the prothoracic gland of these larvae. Cell death is also observed in the ring gland of first instar larvae.
Cele\ced-3UAS.cSa, Scer\GAL4Act88F.PD has visible phenotype, suppressible by BacA\p35UAS.cHa
Cele\ced-3UAS.cSa, Scer\GAL4sca-537.4 has partially lethal - majority die phenotype, suppressible by BacA\p35UAS.cHa, Scer\GAL4sca-537.4
Cele\ced-3UAS.cSa, Scer\GAL4Act88F.PD has visible phenotype, non-suppressible by mir-11UAS.DsRed2, Scer\GAL4Act88F.PD
Cele\ced-3UAS.cSa, Scer\GAL4Act88F.PD has wing blade phenotype, suppressible by BacA\p35UAS.cHa
Cele\ced-3UAS.cSa, Scer\GAL4sca-537.4 has macrochaeta | pharate adult stage phenotype, suppressible by BacA\p35UAS.cHa, Scer\GAL4sca-537.4
Cele\ced-3UAS.cSa, Scer\GAL4Act88F.PD has wing blade phenotype, non-suppressible by mir-11UAS.DsRed2, Scer\GAL4Act88F.PD
Cell death can be blocked by coexpressing BacA\p35Scer\UAS.cHa, using Scer\GAL4sca-537.4.