FlyBase Allele Report: Cele\ced-3[UAS.cSa]

General Information

Symbol

Cele\ced-3^UAS.cSa

Species

C. elegans

Name

Saccharomyces cerevisiae UAS construct a of Shigenaga

FlyBase ID

FBal0089771

Feature type

allele

Associated gene

Cele\ced-3

Associated Insertion(s)

Carried in Construct

P{UAS-Cele\ced-3.S}

Also Known As

UAS-ced3

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Shigenaga et al., 1997)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-Cele\ced-3.S}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of a 2.5kb Cele\ced-3 cDNA that contains entire protein-coding region.

(Shigenaga et al., 1997)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Cele\ced-3

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | embryonic stage, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

increased cell death | larval stage, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

lethal - all die before end of larval stage, with Scer\GAL4^11H-1

(Shigenaga et al., 1997)

lethal - all die before end of pupal stage, with Scer\GAL4³⁸⁹

(Shigenaga et al., 1997)

lethal - all die before end of pupal stage, with Scer\GAL4^ptc-559.1

(Shigenaga et al., 1997)

lethal - all die during larval stage, with Scer\GAL4^srp.D.cCa

(Shia et al., 2009)

partially lethal - majority die, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

some die during larval stage, with Scer\GAL4^11H-1

(Shigenaga et al., 1997)

some die during pupal stage, with Scer\GAL4³⁸⁹

(Shigenaga et al., 1997)

some die during pupal stage, with Scer\GAL4^ptc-559.1

(Shigenaga et al., 1997)

visible, with Scer\GAL4^Act88F.PD

(Truscott et al., 2011, Morris et al., 2006)

Phenotype Manifest In

embryonic/larval glial cell, with Scer\GAL4^rQ286.11

(Sepp et al., 2001)

embryonic/larval hemocyte, with Scer\GAL4^Hml.PG

(Shia et al., 2009)

embryonic/larval hemocyte, with Scer\GAL4^Pxn.PS

(Shia et al., 2009)

embryonic/larval hemocyte, with Scer\GAL4^srp.D.cCa

(Shia et al., 2009)

embryonic/larval nervous system, with Scer\GAL4^rQ286.11

(Sepp et al., 2001)

eye-antennal disc, with Scer\GAL4^ptc-559.1

(Shigenaga et al., 1997)

interommatidial bristle | P-stage, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

macrochaeta & abdomen & pupa, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

macrochaeta & head & pupa, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

macrochaeta & scutum & pupa, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

macrochaeta | pharate adult stage, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

peripheral nervous system, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

prothoracic gland, with Scer\GAL4^11H-1

(Shigenaga et al., 1997)

ring gland, with Scer\GAL4^11H-1

(Shigenaga et al., 1997)

ventral thoracic disc, with Scer\GAL4^ptc-559.1

(Shigenaga et al., 1997)

wing blade, with Scer\GAL4^Act88F.PD

(Truscott et al., 2011, Morris et al., 2006)

wing disc, with Scer\GAL4³⁸⁹

(Shigenaga et al., 1997)

wing disc, with Scer\GAL4^ptc-559.1

(Shigenaga et al., 1997)

wing disc, with Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

Detailed Description

Statement

Reference

Scer\GAL4^Hml.PG-, Scer\GAL4^Pxn.PS- or Scer\GAL4^srp.D.cCa-mediated expression of Cele\ced-3^Scer\UAS.cSa ablates larval hemocytes.

Scer\GAL4^srp.D.cCa-mediated expression of Cele\ced-3^Scer\UAS.cSa leads to a high lethality rate during early larval development - these larvae are devoid of hemocytes and rarely reach the third instar larval stage.

(Shia et al., 2009)

When Cele\ced-3^Scer\UAS.cSa is driven by Scer\GAL4^rQ286.11, to induce ablation of glial cells, a spectrum of nervous system phenotypes are seen in embryonic hemisegments, which range from having a disrupted CNS and PNS pathways to being essentially normal. Embryos that lack a visible glial sheath show defasciculated peripheral nerves as well as axonal pathfinding defects.

(Sepp et al., 2001)

Scer\GAL4^sca-537.4-mediated expression causes lethality at different stages from embryos to pupae and expression of some lines allows viability (Cele\ced-3 is expressed in these lines). Mediated expression can elicit cell death in embryos, as shown by TUNEL. Evidence of ectopic cell death can be seen morphologically: irregular pattern of the peripheral nervous system. Cell death induced in sensory cell anlagen results in a severe defect in the development of sensory bristles: almost all bristles on the notum, head, abdomen and compound eyes of late pupae are missing. Ectopic cell death is not observed in the eye disc, the external morphology and number of ommatidia of the pupal eyes is not affected.

(Shigenaga et al., 1997)

Scer\GAL4^ptc-559.1-mediated expression causes deletion of the scutellum. Wings are not well developed and some of the veins are missing. Arista are missing and the shape of the compound eye is abnormal. Legs are also deleted. Defects are caused by ectopic cell death in the wing disc, lateral regions of the eye disc and in the leg disc. Scer\GAL4³⁸⁹-mediated expression causes wing defects in emerging flies: anterior and posterior regions of the wing are missing. Cell death is induced in two lateral regions of the third instar wing disc. Scer\GAL4^11H-1-mediated expression causes larval lethality or in some lines 'giant larvae' that remain alive for as long as 3 weeks. Most of the cells that secrete ecdysone are missing in the prothoracic gland of these larvae. Cell death is also observed in the ring gland of first instar larvae.

(Shigenaga et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cele\ced-3^UAS.cSa, Scer\GAL4^Act88F.PD has visible phenotype, suppressible by BacA\p35^UAS.cHa

(Morris et al., 2006)

Cele\ced-3^UAS.cSa, Scer\GAL4^sca-537.4 has partially lethal - majority die phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

NOT suppressed by

Statement

Reference

Cele\ced-3^UAS.cSa, Scer\GAL4^Act88F.PD has visible phenotype, non-suppressible by mir-11^UAS.DsRed2, Scer\GAL4^Act88F.PD

(Truscott et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cele\ced-3^UAS.cSa, Scer\GAL4^Act88F.PD has wing blade phenotype, suppressible by BacA\p35^UAS.cHa

(Morris et al., 2006)

Cele\ced-3^UAS.cSa, Scer\GAL4^sca-537.4 has macrochaeta | pharate adult stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^sca-537.4

(Shigenaga et al., 1997)

NOT suppressed by

Statement

Reference

Cele\ced-3^UAS.cSa, Scer\GAL4^Act88F.PD has wing blade phenotype, non-suppressible by mir-11^UAS.DsRed2, Scer\GAL4^Act88F.PD

(Truscott et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Cell death can be blocked by coexpressing BacA\p35^Scer\UAS.cHa, using Scer\GAL4^sca-537.4.

(Shigenaga et al., 1997)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cele\ced-3^Scer\UAS.cSa

Cele\ced-3^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Shigenaga

(Shigenaga et al., 1997)

Secondary FlyBase IDs

References (6)

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