Allele Dmel\rho^Scer\UAS.cBa

General Information
Symbol	Dmel\rho^Scer\UAS.cBa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Bier	FlyBase ID	FBal0090071
Feature type	allele	Associated gene	Dmel\rho

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Buff et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of rho. (Buff et al., 1998)
Carried in construct	P{UAS-ve.B} (Guichard et al., 2002, Ghiglione et al., 2002, Lunde et al., 2003, Gallio et al., 2004, Schulz et al., 2002, Guichard et al., 1999, Guichard et al., 2000, Buff et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^Bx-MS1096 (Guichard et al., 1999)
Phenotype Manifest In
	chorion, with Scer\GAL4^CY2 (Guichard et al., 2000) dorsal appendage, with Scer\GAL4^CY2 (Guichard et al., 2000) eye (Birkholz et al., 2009) ganglionic branch 1 \| embryonic stage, with Scer\GAL4^bs-23.26 (Gallio et al., 2004) ganglionic branch 1 \| embryonic stage, with Scer\GAL4^sim.PS (Gallio et al., 2004) muscle founder cell \| embryonic stage, with Scer\GAL4^twi.PG (Buff et al., 1998) ommatidium (Birkholz et al., 2009) wing, with Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) wing vein, with Scer\GAL4^Bx-MS1096 (Guichard et al., 2002) wing vein \| ectopic, with Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) yellow ommatidium, with Scer\GAL4^GMR.PF (Birkholz et al., 2009)
Detailed Description
	Statement Reference rho[Scer\UAS.cBa]; Scer\GAL4[GMR.PF] adults have rough eyes and the proportion of ommatidia with pale-type R8 cells is significantly increased. Many of these cells are found in ommatidia with a yellow-type R7. (Birkholz et al., 2009) In rho^Scer\UAS.cBa; Scer\GAL4^sim.PS embryos, about a fifth of ganglionic tracheal branch 1s turn posteriorly prematurely, before reaching their characteristic positions close to the midline. Ganglionic branch 1s turn prematurely before reaching the midline in rho^Scer\UAS.cBa; Scer\GAL4^bs-23.26 embryos. Longitudinal fascicles of the ventral nerve cord appear normal in these embryos. (Gallio et al., 2004) The wings of rho^Scer\UAS.cBa; Scer\GAL4^Bx-MS1096 flies have ectopic vein material. (Guichard et al., 2002) When stet^Scer\UAS.cGa is driven by Scer\GAL4^CY2 a strong dorsalisation of the eggshell is seen, resulting in a fused dorsal appendage covering the anterior end of the egg. (Guichard et al., 2000) Misexpression of rho^Scer\UAS.cBa driven by Scer\GAL4^Bx-MS1096 converts almost the entire wing blade into solid vein material and dramatically reduces the size of the wing. (Guichard et al., 1999) Embryos expressing rho^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG show a marked overproduction of eve-positive mesodermal founder cells. (Buff et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has visible phenotype, suppressible by Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999)
NOT Enhancer of
Statement Reference rho^Scer\UAS.cBa, Scer\GAL4^Bx-MS1096 is a non-enhancer of visible phenotype of Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) rho^Scer\UAS.cBa/S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-enhancer of visible phenotype of Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) rho^Scer\UAS.cBa/vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 is a non-enhancer of visible phenotype of Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999)
Suppressor of
Statement Reference rho^Scer\UAS.cBa/Scer\GAL4^kni.L2 is a suppressor of visible phenotype of kni^ri-1 (Lunde et al., 2003)
NOT Suppressor of
Statement Reference rho^Scer\UAS.cBa, Scer\GAL4^Bx-MS1096 is a non-suppressor of visible phenotype of Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) rho^Scer\UAS.cBa/S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-suppressor of visible phenotype of Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) rho^Scer\UAS.cBa/vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 is a non-suppressor of visible phenotype of Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999)
Other
Statement Reference Egfr^Scer\UAS.cBa, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has visible phenotype (Guichard et al., 1999) S^Scer\UAS.cGb, Scer\GAL4^kni.L2, rho^Scer\UAS.cBa has visible phenotype (Lunde et al., 2003, Lunde et al., 2003)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing phenotype, non-enhanceable by S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing phenotype, non-enhanceable by vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L3 phenotype, non-enhanceable by S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L3 phenotype, non-enhanceable by vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L4 phenotype, non-enhanceable by S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L4 phenotype, non-enhanceable by vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L5 phenotype, non-enhanceable by S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L5 phenotype, non-enhanceable by vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein phenotype, non-enhanceable by S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein phenotype, non-enhanceable by vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999)
Suppressed by
Statement Reference Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein \| ectopic \| somatic clone phenotype, suppressible by S^X155 (Guichard et al., 1999) Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein \| ectopic phenotype, suppressible \| partially by S^X155/S[+] (Guichard et al., 1999) Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein phenotype, suppressible by S^DN.Scer\UAS, Scer\GAL4^Bx-MS1096 (Guichard et al., 2002) Scer\GAL4^twi.PG, rho^Scer\UAS.cBa has muscle founder cell \| embryonic stage phenotype, suppressible \| partially by Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^twi.PG (Buff et al., 1998)
NOT suppressed by
Statement Reference Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing phenotype, non-suppressible by S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing phenotype, non-suppressible by vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L3 phenotype, non-suppressible by S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L3 phenotype, non-suppressible by vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L4 phenotype, non-suppressible by S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L4 phenotype, non-suppressible by vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L5 phenotype, non-suppressible by S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein L5 phenotype, non-suppressible by vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein phenotype, non-suppressible by S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999) Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein phenotype, non-suppressible by vn^Scer\UAS.cSa, Scer\GAL4^Bx-MS1096 (Guichard et al., 1999)
NOT Enhancer of
Statement Reference rho^Scer\UAS.cBa, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing phenotype of Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 (Guichard et al., 2000)
Suppressor of
Statement Reference rho^Scer\UAS.cBa/Scer\GAL4^elav.PLu is a suppressor \| partially of embryonic/larval ganglionic branch phenotype of ru^unspecified (Gallio et al., 2004) rho^Scer\UAS.cBa/Scer\GAL4^kni.L2 is a suppressor of wing vein L2 phenotype of kni^ri-1 (Lunde et al., 2003) rho^Scer\UAS.cBa/Scer\GAL4^{nos.UTR.T:Hsim\VP16} is a suppressor \| partially of testis phenotype of stet^unspecified (Schulz et al., 2002) rho^Scer\UAS.cBa/Scer\GAL4^sim.PS is a suppressor \| partially of embryonic/larval ganglionic branch phenotype of ru^unspecified (Gallio et al., 2004) rho^Scer\UAS.cBa/Scer\GAL4^sli.PS is a suppressor \| partially of embryonic/larval ganglionic branch phenotype of ru^unspecified (Gallio et al., 2004)
NOT Suppressor of
Statement Reference rho^Scer\UAS.cBa, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing phenotype of Egfr^{DN.Scer\UAS.cBa}, Scer\GAL4^Bx-MS1096 (Guichard et al., 2000)
Other
Statement Reference Egfr^Scer\UAS.cBa, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing phenotype (Guichard et al., 1999) Egfr^Scer\UAS.cBa, Scer\GAL4^Bx-MS1096, rho^Scer\UAS.cBa has wing vein phenotype (Guichard et al., 1999) S^Scer\UAS.cGb, Scer\GAL4^kni.L2, rho^Scer\UAS.cBa has wing phenotype (Lunde et al., 2003, Lunde et al., 2003) S^Scer\UAS.cGb, Scer\GAL4^kni.L2, rho^Scer\UAS.cBa has wing vein \| ectopic phenotype (Lunde et al., 2003, Lunde et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference rho^Scer\UAS.cBa; Scer\GAL4^sli.PS and rho^Scer\UAS.cBa; Scer\GAL4^sim.PS partially suppress ventral midline crossing by ganglionic tracheal branches in ru^unspecified mutant stage 16 embryos. (Gallio et al., 2004) Expression of rho^Scer\UAS.cBa under the control of Scer\GAL4^kni.L2 rescues the L2 wing vein phenotype of kni^ri-1 flies. Co-expression of S^Scer\UAS.cGb and rho^Scer\UAS.cBa under the control of Scer\GAL4^kni.L2 results in ectopic veins that are displaced from the endogenous L2 wing vein by a few cells. (Lunde et al., 2003) Co-expression of Egfr^{DN.Scer\UAS.cBa} and rho^Scer\UAS.cBa driven by Scer\GAL4^Bx-MS1096 in females deletes parts of dorsal veins L3, L5 and the distal portion of L4, reduces the width of the wing, and causes mis-alignment between dorsal and ventral components of the veins. This is an almost identical phenotype to Scer\GAL4^Bx-MS1096 driven Egfr^{DN.Scer\UAS.cBa} expression alone. In males a stronger vein-loss phenotype is seen. Co-expression of Egfr^{DN.Scer\UAS.cBa}, Egfr^Scer\UAS.cBa and rho^Scer\UAS.cBa driven by Scer\GAL4^Bx-MS1096 fully restores the rho^Scer\UAS.cBa induced ectopic vein phenotype. Overexpression of rho^Scer\UAS.cBa driven by Scer\GAL4^Bx-MS1096 in S^X155 heterozygotes, leads to a partial suppression of the rho^Scer\UAS.cBa induced solid vein phenotype. Overexpression of rho^Scer\UAS.cBa driven by Scer\GAL4^Bx-MS1096 in S^X155 homozygous clones, produced a S^X155 type phenotype, the rho^Scer\UAS.cBa induced solid wing phenotype is completely suppressed. (Guichard et al., 1999) The overproduction of eve-positive mesodermal founder cells seen in embryos expressing rho^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG is partly suppressed if they are also carrying Egfr^{DN.Scer\UAS.cBa}. (Buff et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	E. Bier

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	rho^Scer\UAS.cBa ve^Scer\UAS.cBa (Guichard et al., 1999, ) ve^UAS.cBa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Bier (Buff et al., 1998)
Secondary FlyBase IDs

References ( 9 )
Research paper	Birkholz et al., 2009, J. Neurosci. 29(9): 2666--2675 rhomboid mediates specification of blue- and green-sensitive R8 photoreceptor cells in Drosophila. [FBrf0207540] Gallio et al., 2004, Development 131(15): 3605--3614 Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline. [FBrf0179216] Lunde et al., 2003, Development 130(2): 235--248 Activation of the knirps locus links patterning to morphogenesis of the second wing vein in Drosophila. [FBrf0155705] Ghiglione et al., 2002, Development 129(1): 175--186 Mechanism of activation of the Drosophila EGF Receptor by the TGFalpha ligand Gurken during oogenesis. [FBrf0141506] Guichard et al., 2002, Proc. Natl. Acad. Sci. U.S.A. 99(6): 3752--3757 A screen for dominant mutations applied to components in the Drosophila EGF-R pathway. [FBrf0147188] Schulz et al., 2002, Development 129(19): 4523--4534 Signaling from germ cells mediated by the rhomboid homolog stet organizes encapsulation by somatic support cells. [FBrf0151926] Guichard et al., 2000, Dev. Biol. 226(2): 255--266 brother of rhomboid, a rhomboid-related gene expressed during early Drosophila oogenesis, promotes EGF-R/MAPK signaling. [FBrf0129841] Guichard et al., 1999, Development 126(12): 2663--2676 rhomboid and Star interact synergistically to promote EGFR/MAPK signaling during Drosophila wing vein development. [FBrf0108778] Buff et al., 1998, Development 125(11): 2075--2086 Signalling by the Drosophila epidermal growth factor receptor is required for the specification and diversification of embryonic muscle progenitors. [FBrf0102731]

Allele Dmel\rhoScer\UAS.cBa

Allele Dmel\rho^Scer\UAS.cBa