Allele Dmel\tkv^{Q199D.Scer\UAS}

General Information
Symbol	Dmel\tkv^{Q199D.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0090090
Feature type	allele	Associated gene	Dmel\tkv

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Das and Cho, 1998.6.17) in vitro construct - regulatory fusion (Das and Cho, 1998.6.17)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Amino acid replacement: Q199D. Scer\UAS regulatory sequences drive expression of a mutated form of tkv. (Das and Cho, 1998.6.17)
Carried in construct	P{UAS-tkv.Q199D.C} (de Navascués and Modolell, 2007, Kawase et al., 2004, Xie and Spradling, 1998, Das and Cho, 1998.6.17, Layden et al., 2006, Kirilly et al., 2005, Galindo et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	egg chamber, with Scer\GAL4^hs.PB (Xie and Spradling, 1998)
Detailed Description
	Statement Reference When neutral marked clones are induced in the ovary, the proportion of germaria carrying marked somatic stem cells 3 weeks after clone induction is around 40% of that seen one week after clone induction. However, for tkv[Q199D.Scer\UAS] Scer\GAL4[Act5C.PI] clones induced using this method, this increases to 65.6%. Also, while the number of germaria containing tkv[Q199D.Scer\UAS] Scer\GAL4[Act5C.PI] clones in somatic stem cells decreases over time after induction, the number of germaria carrying only marked somatic stem cells actually increases over time after induction from 0.59% after one week to 8.9% after 3 weeks. (Kirilly et al., 2005) When expression is driven by Scer\GAL4^hs.PB in the somatic cells of the germarium, no germline tumor phenotype is observed. Egg chamber budding was affected, indicating a later effect on somatic cell function. (Xie and Spradling, 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, tkv^{Q199D.Scer\UAS} is a suppressor \| partially of follicle stem cell \| somatic clone phenotype of dsh³ (Kirilly et al., 2005)
NOT Suppressor of
Statement Reference Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, tkv^{Q199D.Scer\UAS} is a non-suppressor \| somatic clone of follicle stem cell \| somatic clone phenotype of smo^D16 (Kirilly et al., 2005) Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, tkv^{Q199D.Scer\UAS} is a non-suppressor of follicle stem cell \| somatic clone phenotype of smo³ (Kirilly et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	S.H. Cho

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	tkv^{Q199D.Scer\UAS} tkv^Q199D.UAS
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Galindo et al., 2011, Dev. Biol. 353(2): 396--410 Control of Distal-less expression in the Drosophila appendages by functional 3' enhancers. [FBrf0213529] de Navascués and Modolell, 2007, Development 134(9): 1779--1788 tailup, a LIM-HD gene, and Iro-C cooperate in Drosophila dorsal mesothorax specification. [FBrf0201638] Layden et al., 2006, Dev. Biol. 291(2): 253--263 Zfh1, a somatic motor neuron transcription factor, regulates axon exit from the CNS. [FBrf0190136] Kirilly et al., 2005, Dev. Cell 9(5): 651--662 BMP signaling is required for controlling somatic stem cell self-renewal in the Drosophila ovary. [FBrf0190209] Kawase et al., 2004, Development 131(6): 1365--1375 Gbb/Bmp signaling is essential for maintaining germline stem cells and for repressing bam transcription in the Drosophila testis. [FBrf0174556] Xie and Spradling, 1998, Cell 94(2): 251--260 decapentaplegic is essential for the maintenance and division of germline stem cells in the Drosophila ovary. [FBrf0103396]
Personal communication to FlyBase	Das and Cho, 1998.6.17, tkv construct. tkv construct. [FBrf0102810]

Allele Dmel\tkvQ199D.Scer\UAS

Allele Dmel\tkv^{Q199D.Scer\UAS}