FlyBase Allele Report: Dmel\sgg[UAS.cSa]

Adult eyes expressing sgg^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PS display a slight rough-eye phenotype. Retinal sections show a relatively normal array of rhabdomeres other than some abnormal polarity.

(Chatterjee et al., 2009)

Scer\GAL4^vg.PU-driven overexpression of sgg^Scer\UAS.cSa in the wing imaginal disc results in loss of bristles and marginal tissue in the adult wing.

(Kalamegham et al., 2007)

Expression of sgg^Scer\UAS.cSa under the control of Scer\GAL4^vg.PM leads to a reduction in the wing pouch size within the dorsal mesothoracic disc.

(Mohit et al., 2006)

sgg^Scer\UAS.cSa; Scer\GAL4^vg.PM flies have reduced, serrated wings. 30% of these flies show partial wing to notum transformation.

(Bajpai et al., 2004)

Flies expressing sgg^Scer\UAS.cSa under the control of Scer\GAL4^sd-SG29.1 show loss of wing and duplication of the dorsal thorax.

(Giraldez et al., 2002)

When sgg^Scer\UAS.cSa is driven by Scer\GAL4^elav-C155, no visible external phenotype is seen other than mildly disordered bristles.

(Jackson et al., 2002)

Expression of sgg^Scer\UAS.cSa under the control of Scer\GAL4^Bx-MS1096 results in wing hinge defects; axillary sclerite 3 is missing, while axillary sclerite 1, axillary sclerite 2, axillary sclerite 4 and the unnamed plate are unaffected. Wings from these animals are very small and lack marginal structures. Expression of sgg^Scer\UAS.cSa under the control of Scer\GAL4^dpp.blk1 results in duplication of the axillary pouch.

(Buratovich and Wilder, 2001)

Expression of sgg^Scer\UAS.cSa under the control of Scer\GAL4^da.G32 results in the loss of 2 to 4 rows of 4^o cells without affecting the hh-dependent denticles in the dorsal epidermis of the embryo (in wild-type embryos each parasegment contains 4 types of cells in the dorsal epidermis; the 1^o, 2^o and 3^o cells in the anterior half of the parasegment and the 4^o cells in the posterior half of the parasegment).

(Buratovich et al., 2000)

Enhances the wing nicking phenotype seen in mam^N.Scer\UAS, Scer\GAL4^C96 flies.

(Helms et al., 1999)

Embryos expressing sgg^Scer\UAS.cSa under the control of Scer\GAL4^69B are viable but have a subtle cuticle phenotype; a few extra denticles are seen in regions of ventral cuticle that are normally naked. Similar phenotypes are seen when the Scer\GAL4^da.G32 driver is used instead of Scer\GAL4^69B. Animals expressing one copy of sgg^Scer\UAS.cSa under the control of Scer\GAL4^how-24B at 25^oC die as first or second instar larvae. The larvae hatch at a normal frequency and survive for 2-3 days but lack normal mobility. The somatic muscles, midgut and fat bodies appear superficially normal in late embryos of this genotype. Few or no copper cells are seen in the midgut of first instar larvae expressing sgg^Scer\UAS.cSa under the control of Scer\GAL4^how-24B. The interstitial cells are present. There is no anterior expansion of flat cell fate (these cells are normally found just posterior to the copper cells). Approximately 3/4 of animals expressing two copies of sgg^Scer\UAS.cSa under the control of Scer\GAL4^how-24B at 29^oC die as embryos, with the rest dying as first instar larvae. Copper cell differentiation is blocked in animals that survive to the first larval instar stage. The second midgut constriction often fails to form.

(Steitz et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sgg^UAS.cSa, Scer\GAL4^GMR.PS is an enhancer of abnormal size phenotype of Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

sgg^UAS.cSa, Scer\GAL4^GMR.PS is an enhancer of abnormal planar polarity phenotype of Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^GMR.PS is an enhancer of abnormal size phenotype of Hsap\MAPT^GMR.Ex.PJ

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^GMR.PS is an enhancer of abnormal planar polarity phenotype of Hsap\MAPT^GMR.Ex.PJ

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^GMR.PS is an enhancer of abnormal size phenotype of Hsap\MAPT^GMR.S11A

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^elav-C155 is an enhancer of visible phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

sgg^UAS.cSa/Scer\GAL4^bbg-C96 is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^vg.PM, sgg^UAS.cSa has mesonotum | ectopic phenotype, enhanceable by tws[+]/tws^j11C8

(Bajpai et al., 2004)

Scer\GAL4^vg.PM, sgg^UAS.cSa has wing phenotype, enhanceable by tws[+]/tws^j11C8

(Bajpai et al., 2004)

Scer\GAL4^vg.PM, sgg^UAS.cSa has wing margin phenotype, enhanceable by tws[+]/tws^j11C8

(Bajpai et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^vg.PM, sgg^UAS.cSa has wing disc phenotype, suppressible by Cyp310a1^RNAi.Sym.UAS, Scer\GAL4^vg.PM

(Mohit et al., 2006)

Scer\GAL4^vg.PM, sgg^UAS.cSa has wing disc phenotype, suppressible by Scer\GAL4^vg.PM/peg^RNAi.Sym.UAS

(Mohit et al., 2006)

Enhancer of

Statement

Reference

sgg^UAS.cSa, Scer\GAL4^GMR.PS is an enhancer of interommatidial bristle phenotype of Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

sgg^UAS.cSa, Scer\GAL4^GMR.PS is an enhancer of rhabdomere phenotype of Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^GMR.PS is an enhancer of ommatidium phenotype of Hsap\MAPT^GMR.Ex.PJ

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^GMR.PS is an enhancer of eye phenotype of Hsap\MAPT^GMR.Ex.PJ

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^GMR.PS is an enhancer of interommatidial bristle phenotype of Hsap\MAPT^GMR.Ex.PJ

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^GMR.PS is an enhancer of rhabdomere phenotype of Hsap\MAPT^GMR.Ex.PJ

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^GMR.PS is an enhancer of ommatidium phenotype of Hsap\MAPT^GMR.S11A

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^GMR.PS is an enhancer of eye phenotype of Hsap\MAPT^GMR.S11A

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^GMR.PS is an enhancer of interommatidial bristle phenotype of Hsap\MAPT^GMR.S11A

(Chatterjee et al., 2009)

sgg^UAS.cSa, Scer\GAL4^GMR.PS is an enhancer of ommatidium phenotype of Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

sgg^UAS.cSa, Scer\GAL4^GMR.PS is an enhancer of eye phenotype of Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

sgg^UAS.cSa/Scer\GAL4^elav-C155 is an enhancer of eye phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

sgg^UAS.cSa/Scer\GAL4^elav-C155 is an enhancer of ommatidium phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

sgg^UAS.cSa/Scer\GAL4^bbg-C96 is an enhancer of wing phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

NOT Enhancer of

Statement

Reference

sgg^UAS.cSa, Scer\GAL4^GMR.PS is a non-enhancer of interommatidial bristle phenotype of Hsap\MAPT^UAS.S2A, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

sgg^UAS.cSa, Scer\GAL4^GMR.PS is a non-enhancer of eye phenotype of Hsap\MAPT^UAS.S2A, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

sgg^UAS.cSa, Scer\GAL4^GMR.PS is a non-enhancer of retina phenotype of Hsap\MAPT^UAS.S2A, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Suppressor of

Statement

Reference

sgg^UAS.cSa/Scer\GAL4^elav-C155 is a suppressor of eye photoreceptor cell phenotype of Apc^Q8

(Ahmed et al., 1998)

NOT Suppressor of

Statement

Reference

sgg^UAS.cSa/Scer\GAL4^how-24B is a non-suppressor of embryonic midgut constriction 2 phenotype of Scer\GAL4^how-24B, arm^{S10.UAS.Tag:MYC}

(Steitz et al., 1998)

Other

Statement

Reference

Hsap\MAPT^GMR.Ex.PJ, Scer\GAL4^elav-C155, sgg^UAS.cSa has lamina phenotype

(Jackson et al., 2002)

Hsap\MAPT^GMR.Ex.PJ, Scer\GAL4^elav-C155, sgg^UAS.cSa has retina phenotype

(Jackson et al., 2002)

Hsap\MAPT^GMR.Ex.PJ, Scer\GAL4^elav-C155, sgg^UAS.cSa has medulla phenotype

(Jackson et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of Cyp310a1^{dsRNA.Sym.Scer\UAS} or CG17278^{dsRNA.Sym.Scer\UAS} to create Cyp310a1 or CG17278 dsRNA results in a suppression of the reduction in wing pouch size seen when sgg^Scer\UAS.cSa is expressed under the control of Scer\GAL4^vg.PM.

(Mohit et al., 2006)

The wing phenotypes of sgg^Scer\UAS.cSa; Scer\GAL4^vg.PM flies are enhanced by tws^j11C8/+: complete wing to notum transformation occurs in 87% of these flies.

(Bajpai et al., 2004)

Many photoreceptors are rescued from death in Apc^Q8 homozygotes carrying one copy of sgg^Scer\UAS.cSa expressed under the control of Scer\GAL4^elav-C155.

(Ahmed et al., 1998)

Does not alter the abnormal second midgut constriction phenotype seen in embryos expressing arm^{S10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^how-24B.

(Steitz et al., 1998)

Xenogenetic Interactions

Statement

Reference

Co-expression of sgg^Scer\UAS.cSa markedly enhances the Hsap\MAPT^Scer\UAS.wt, Scer\GAL4^GMR.PS eye phenotype, results in smaller eye with abnormally fused ommatidia and complete loss of bristles. The internal retinal architecture of the double transgenics is severely disrupted.

The relatively normal eye phenotype of Hsap\MAPT^Scer\UAS.S2A, Scer\GAL4^GMR.PS flies remains unchanged when sgg^Scer\UAS.cSa is co-expressed.

The eye and retinal phenotypes seen in Hsap\MAPT^GMR.Ex.PJ animals are enhanced by Scer\GAL4^GMR.PS-mediated co-expression of sgg^Scer\UAS.cSa.

The Hsap\MAPT^GMR.S11A small and rough eye phenotype is enhanced by Scer\GAL4^GMR.PS-mediated co-expression of sgg^Scer\UAS.cSa. Retinas of the double transgenics show dramatic disruption, and rhabdomeres show disorganization with some photoreceptor loss.

(Chatterjee et al., 2009)

When sgg^Scer\UAS.cSa (when driven by Scer\GAL4^elav-C155) is added to Hsap\MAPT^GMR.Ex.PJ, the eye phenotype is enhanced. The eye is markedly reduced and there is an almost complete loss of normal ommatidia and a loss of the majority of bristles. Abundant discrete flam-shaped and fibrillar aggregates and vacuolisation are observed throughout the retina and underlying lamina and medulla.

(Jackson et al., 2002)

Complementation and Rescue Data

Partially rescues

sgg^UAS.cSa/Scer\GAL4^arm.PS partially rescues sgg^M11

(Kalamegham et al., 2007)

sgg^UAS.cSa/Scer\GAL4^69B partially rescues sgg^M11

(Steitz et al., 1998)

Comments

Zygotic expression of sgg^Scer\UAS.cSa driven by Scer\GAL4^arm.PS in maternal mutant sgg^M11 embryos partially restores segmentation.

(Kalamegham et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sgg^Scer\UAS.cSa

sgg^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Steitz

(Steitz et al., 1998)

Secondary FlyBase IDs

References (15)

Report Sections

Open Close