Adult eyes expressing sggScer\UAS.cSa under the control of Scer\GAL4GMR.PS display a slight rough-eye phenotype. Retinal sections show a relatively normal array of rhabdomeres other than some abnormal polarity.
Scer\GAL4vg.PU-driven overexpression of sggScer\UAS.cSa in the wing imaginal disc results in loss of bristles and marginal tissue in the adult wing.
Expression of sggScer\UAS.cSa under the control of Scer\GAL4vg.PM leads to a reduction in the wing pouch size within the dorsal mesothoracic disc.
Expression of sggScer\UAS.cSa under the control of Scer\GAL4Bx-MS1096 results in wing hinge defects; axillary sclerite 3 is missing, while axillary sclerite 1, axillary sclerite 2, axillary sclerite 4 and the unnamed plate are unaffected. Wings from these animals are very small and lack marginal structures. Expression of sggScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 results in duplication of the axillary pouch.
Expression of sggScer\UAS.cSa under the control of Scer\GAL4da.G32 results in the loss of 2 to 4 rows of 4o cells without affecting the hh-dependent denticles in the dorsal epidermis of the embryo (in wild-type embryos each parasegment contains 4 types of cells in the dorsal epidermis; the 1o, 2o and 3o cells in the anterior half of the parasegment and the 4o cells in the posterior half of the parasegment).
Embryos expressing sggScer\UAS.cSa under the control of Scer\GAL469B are viable but have a subtle cuticle phenotype; a few extra denticles are seen in regions of ventral cuticle that are normally naked. Similar phenotypes are seen when the Scer\GAL4da.G32 driver is used instead of Scer\GAL469B. Animals expressing one copy of sggScer\UAS.cSa under the control of Scer\GAL4how-24B at 25oC die as first or second instar larvae. The larvae hatch at a normal frequency and survive for 2-3 days but lack normal mobility. The somatic muscles, midgut and fat bodies appear superficially normal in late embryos of this genotype. Few or no copper cells are seen in the midgut of first instar larvae expressing sggScer\UAS.cSa under the control of Scer\GAL4how-24B. The interstitial cells are present. There is no anterior expansion of flat cell fate (these cells are normally found just posterior to the copper cells). Approximately 3/4 of animals expressing two copies of sggScer\UAS.cSa under the control of Scer\GAL4how-24B at 29oC die as embryos, with the rest dying as first instar larvae. Copper cell differentiation is blocked in animals that survive to the first larval instar stage. The second midgut constriction often fails to form.