|Name||Saccharomyces cerevisiae UAS construct a of True||FlyBase ID||FBal0090174|
|Feature type||allele||Associated gene||Dmel\ple|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
A 2628bp EcoRI-HinDIII fragment containing the complete ple open reading frame is driven by Scer\UAS sequences.
|Carried in construct|
|Phenotype Manifest In|
Flies expressing ple[Scer\UAS.cTa] under the control of Scer\GAL4[ple.PF] show normal temperature-preference behaviour.
When pleScer\UAS.cTa is driven by Scer\GAL4ap-md544, Scer\GAL4ap-md544, Scer\GAL4Dll-md23 or Scer\GAL4en-e16E, no ectopic pigmentation is seen. If pleScer\UAS.cTa is driven by Scer\GAL4hs.2sev (with no heatshock) or Scer\GAL4how-24B, a gradual development of ectopic brown stripes in the intervein areas of the wings during adult life was seen.
Scer\GAL4hs.2sev, pleScer\UAS.cTa has body color defective phenotype, enhanceable by DdcScer\UAS.cTa, Scer\GAL4hs.2sev
|Phenotype Manifest In|
Flies co-expressing both Ddc[Scer\UAS.cTa] and ple[Scer\UAS.cTa] under the control of Scer\GAL4[ple.PF] show abnormal temperature-preference behaviour; their avoidance index against high temperatures is only 39% of wild type.
Co-expressing pleScer\UAS.cTa and DdcScer\UAS.cTa under the control of Scer\GAL4hs.2sev leads to a more intense ectopic pigmentation phenotype than pleScer\UAS.cTa alone. The adult cuticle remains underpigmented.
|Complementation & Rescue Data|
|Stocks ( 1 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of True
|Secondary FlyBase IDs|
|References ( 4 )|