Allele Dmel\ple^Scer\UAS.cTa

General Information
Symbol	Dmel\ple^Scer\UAS.cTa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of True	FlyBase ID	FBal0090174
Feature type	allele	Associated gene	Dmel\ple

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (True and Carroll, 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A 2628bp EcoRI-HinDIII fragment containing the complete ple open reading frame is driven by Scer\UAS sequences. (True et al., 1999) Construct: Scer\UAS sequences drive ple expression. (True and Carroll, 1998)
Carried in construct	P{UAS-ple.T} (True et al., 1999, True and Carroll, 1998, Bang et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	body color defective, with Scer\GAL4^hs.2sev (True et al., 1999) body color defective, with Scer\GAL4^unspecified (True and Carroll, 1998)
Phenotype Manifest In
	adult abdomen, with Scer\GAL4^unspecified (True and Carroll, 1998) wing cell, with Scer\GAL4^unspecified (True and Carroll, 1998)
Detailed Description
	Statement Reference Flies expressing ple[Scer\UAS.cTa] under the control of Scer\GAL4[ple.PF] show normal temperature-preference behaviour. (Bang et al., 2011) When ple^Scer\UAS.cTa is driven by Scer\GAL4^ap-md544, Scer\GAL4^ap-md544, Scer\GAL4^Dll-md23 or Scer\GAL4^en-e16E, no ectopic pigmentation is seen. If ple^Scer\UAS.cTa is driven by Scer\GAL4^hs.2sev (with no heatshock) or Scer\GAL4^how-24B, a gradual development of ectopic brown stripes in the intervein areas of the wings during adult life was seen. (True et al., 1999) Expression of ple^Scer\UAS.cTa under the control of Scer\GAL4^unspecified results in lightening of the adult abdominal stripes and a low level of ectopic pigmentation in the intervein areas of the wing. (True and Carroll, 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^hs.2sev, ple^Scer\UAS.cTa has body color defective phenotype, enhanceable by Ddc^Scer\UAS.cTa, Scer\GAL4^hs.2sev (True et al., 1999) Scer\GAL4^unspecified, ple^Scer\UAS.cTa has body color defective phenotype, enhanceable by Ddc^Scer\UAS.cTa, Scer\GAL4^unspecified (True and Carroll, 1998)
Suppressed by
Statement Reference Scer\GAL4^unspecified, ple^Scer\UAS.cTa has body color defective phenotype, suppressible by Ddc^Scer\UAS.cTa, Scer\GAL4^unspecified (True and Carroll, 1998)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^unspecified, ple^Scer\UAS.cTa has wing cell phenotype, enhanceable by Ddc^Scer\UAS.cTa, Scer\GAL4^unspecified (True and Carroll, 1998)
Suppressed by
Statement Reference Scer\GAL4^unspecified, ple^Scer\UAS.cTa has adult abdomen phenotype, suppressible by Ddc^Scer\UAS.cTa, Scer\GAL4^unspecified (True and Carroll, 1998)
Additional Comments
Genetic Interactions
Statement Reference Flies co-expressing both Ddc[Scer\UAS.cTa] and ple[Scer\UAS.cTa] under the control of Scer\GAL4[ple.PF] show abnormal temperature-preference behaviour; their avoidance index against high temperatures is only 39% of wild type. (Bang et al., 2011) Co-expressing ple^Scer\UAS.cTa and Ddc^Scer\UAS.cTa under the control of Scer\GAL4^hs.2sev leads to a more intense ectopic pigmentation phenotype than ple^Scer\UAS.cTa alone. The adult cuticle remains underpigmented. (True et al., 1999) Expression of ple^Scer\UAS.cTa together with Ddc^Scer\UAS.cTa under the control of Scer\GAL4^unspecified enhances the wing pigmentation phenotype and variably suppresses the abdominal pigmentation phenotype. (True and Carroll, 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	37539 w^*; P{UAS-ple.T}331f2
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	ple^Scer\UAS.cTa ple^UAS.cTa
Name Synonym	Saccharomyces cerevisiae UAS construct a of True (True and Carroll, 1998)
Secondary FlyBase IDs

References ( 4 )
Research paper	Bang et al., 2011, PLoS Genet. 7(3): e1001346 Dopamine signalling in mushroom bodies regulates temperature-preference behaviour in Drosophila. [FBrf0213392] True et al., 1999, Curr. Biol. 9(23): 1382--1391 Drosophila wing melanin patterns form by vein-dependent elaboration of enzymatic prepatterns. [FBrf0123221]
Review	O'Grady and DeSalle, 2000, Curr. Biol. 10(2): R75--R77 How the fruit fly changed (some of) its spots. [FBrf0125815]
Abstract	True and Carroll, 1998, Dev. Biol. 198(1): 199 Genetic analysis of melanin patterning in Drosophila. [FBrf0102773]

Allele Dmel\pleScer\UAS.cTa

Allele Dmel\ple^Scer\UAS.cTa