Allele Dmel\htl^Scer\UAS.cMa

General Information
Symbol	Dmel\htl^Scer\UAS.cMa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Michelson	FlyBase ID	FBal0090412
Feature type	allele	Associated gene	Dmel\htl

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Michelson et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length htl is expressed under the control of Scer\UAS regulatory sequences. (Michelson et al., 1998)
Carried in construct	P{UAS-htl.M} (Kumar and Moses, 2001, Stathopoulos et al., 2004, Kumar and Moses, 2001, Garcia-Alonso et al., 2000, Michelson, 1999.8.9, Wilson et al., 2005, Schumacher et al., 2004, Bradley et al., 2003, Michelson et al., 1998, Maqbool et al., 2006, Reim et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	axon & mechanosensory neuron & adult head, with Scer\GAL4^Bx-MS1096, Scer\GAL4^Mz1277, Scer\GAL4^twi.PB, htl^YY262/htl^AB42 (Garcia-Alonso et al., 2000) axon & ocellus sensory structure, with Scer\GAL4^Bx-MS1096, Scer\GAL4^Mz1277, Scer\GAL4^twi.PB, htl^YY262/htl^AB42 (Garcia-Alonso et al., 2000) muscle fiber \| supernumerary, with Scer\GAL4¹¹⁵¹ (Maqbool et al., 2006) myoblast \| supernumerary, with Scer\GAL4¹¹⁵¹ (Maqbool et al., 2006) tarsal levator muscle, with Scer\GAL4¹¹⁵¹ (Maqbool et al., 2006)
Detailed Description
	Statement Reference Expression of htl[Scer\UAS.cMa] in developing myoblasts under the control of Scer\GAL4[1151] results in an increase in the number of myoblasts (specifically tilm myoblasts). Consistent with these larval phenotypes, over-expression leads to a significantly higher number of fibres contributing to leg muscles. For example, the average number of htl[Scer\UAS.cMa]-induced muscle fibres (counted in 10 adult legs) of the tarsus levator muscle (talm) is 14.8 compared to 10 fibres in the wild-type. However, the overall leg muscle pattern and capacity of fibres to interact with internal tendons appears unchanged. (Maqbool et al., 2006) Expression of htl^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1 has no effect on furrow formation in the eye disc. (Kumar and Moses, 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference htl^Scer\UAS.cMa/Scer\GAL4^MS1075 is a suppressor \| partially of axon & adult head \| conditional ts phenotype of Nrg^l10 (Garcia-Alonso et al., 2000)
NOT Suppressor of
Statement Reference htl^Scer\UAS.cMa/Scer\GAL4^twi.PB is a non-suppressor of mesoderm phenotype of pbl³ (Schumacher et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Mesoderm spreading and differentiation in pbl³ homozygous embryos is not rescued by htl^Scer\UAS.cMa; Scer\GAL4^twi.PB. (Schumacher et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	htl^Scer\UAS.cMa/Scer\GAL4^twi.PB rescues htl^AB42 (Schumacher et al., 2004) htl^Scer\UAS.cMa/Scer\GAL4^twi.PG rescues htl^AB42 (Michelson et al., 1998, Wilson et al., 2005, Bradley et al., 2003)
Partially rescues	htl^Scer\UAS.cMa, Scer\GAL4^twi.PB, Scer\GAL4^Mz1277, Scer\GAL4^Bx-MS1096 partially rescues htl^YY262/htl^AB42 (Garcia-Alonso et al., 2000) htl^Scer\UAS.cMa/Scer\GAL4^tey-5053A partially rescues htl^AB42 (Reim et al., 2012)
Fails to rescue	htl^Scer\UAS.cMa/Scer\GAL4^twi.PB fails to rescue htl^YY262/htl^AB42 (Garcia-Alonso et al., 2000)
Comments	Expression of htl[Scer\UAS.cMa] under the control of Scer\GAL4[tey-5053A] rescues the longitudinal visceral muscle (LVM) founder cell death seen in htl[AB42] mutant stage 13 embryos. The founder cell migration defects are also partially rescued, however some cells acquire abnormal shapes, adhere to positions more distant from the TVM both dorsally and ventrally, and form clusters, resulting in missing LVM cells in the most anterior trunk segments. (Reim et al., 2012) Expression of htl^Scer\UAS.cMa under the control of Scer\GAL4^twi.PG is able to induce bilaterally symmetrical flattening of the mesodermal tube onto the ectoderm in the early stages of mesoderm morphogenesis in htl^AB42 embryos. (Wilson et al., 2005) htl^Scer\UAS.cMa; Scer\GAL4^twi.PB rescues the migration of the mesoderm after invagination in htl^AB42 embryos. As a result the number of hemisegments with eve expressing mesodermal cells is 22, just as in wild-type. (Schumacher et al., 2004) Expression of htl^Scer\UAS.cMa under the control of Scer\GAL4^twi.PG rescues the formation of the visceral mesoderm and salivary gland migration in htl^AB42/htl^AB42 embryos. (Bradley et al., 2003) The addition of htl^Scer\UAS.cMa driven by Scer\GAL4^twi.PB fails to rescue the lethality seen in htl^YY262/htl^AB42. However, when htl^Scer\UAS.cMa is driven by Scer\GAL4^Mz1277, Scer\GAL4^Bx-MS1096 and Scer\GAL4^twi.PB concurrently, some escaper pupae do survive. (Garcia-Alonso et al., 2000) The addition of htl^Scer\UAS.cMa driven by Scer\GAL4^twi.PB, fails rescue the lethality seen in htl^YY262/htl^AB42. However, when htl^Scer\UAS.cMa is driven by Scer\GAL4^Mz1277, Scer\GAL4^Bx-MS1096 and Scer\GAL4^twi.PB concurrently some escaper pupae do survive. (Garcia-Alonso et al., 2000)
Stocks ( 2 )
Bloomington	5419 y¹ w^*; P{UAS-htl.M}YYDFR-F16
Kyoto	108219 y¹ w^*; P{UAS-htl.M}YYDFR-F16
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	htl^Scer\UAS.cMa htl^UAS.cMa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Michelson (Michelson et al., 1998)
Secondary FlyBase IDs

References ( 11 )
Research paper	Reim et al., 2012, Dev. Biol. 368(1): 28--43 The FGF8-related signals Pyramus and Thisbe promote pathfinding, substrate adhesion, and survival of migrating longitudinal gut muscle founder cells. [FBrf0218673] Maqbool et al., 2006, PLoS ONE 1(1): e122 Shaping Leg Muscles in Drosophila: Role of ladybird, a Conserved Regulator of Appendicular Myogenesis. [FBrf0193570] Wilson et al., 2005, Development 132(3): 491--501 FGF signalling and the mechanism of mesoderm spreading in Drosophila embryos. [FBrf0183935] Schumacher et al., 2004, Development 131(11): 2631--2640 The RhoGEF Pebble is required for cell shape changes during cell migration triggered by the Drosophila FGF receptor Heartless. [FBrf0179437] Stathopoulos et al., 2004, Genes Dev. 18(6): 687--699 pyramus and thisbe: fGF genes that pattern the mesoderm of Drosophila embryos. [FBrf0174649] Bradley et al., 2003, Dev. Biol. 257(2): 249--262 Posterior migration of the salivary gland requires an intact visceral mesoderm and integrin function. [FBrf0158855] Kumar and Moses, 2001, Development 128(14): 2689--2697 The EGF receptor and notch signaling pathways control the initiation of the morphogenetic furrow during Drosophila eye development. [FBrf0138358] Kumar and Moses, 2001, Cell 104(5): 687--697 EGF receptor and Notch signaling act upstream of Eyeless/Pax6 to control eye specification. [FBrf0134490] Garcia-Alonso et al., 2000, Neuron 28(3): 741--752 The EGF and FGF receptors mediate neuroglian function to control growth cone decisions during sensory axon guidance in Drosophila. [FBrf0132430] Michelson et al., 1998, Dev. Genet. 22(3): 212--229 Dual functions of the heartless fibroblast growth factor receptor in development of the Drosophila embryonic mesoderm. [FBrf0102648]
Personal communication to FlyBase	Michelson, 1999.8.9, UAS constructs and insertions from Alan Michelson. UAS constructs and insertions from Alan Michelson. [FBrf0109187]

Allele Dmel\htlScer\UAS.cMa

Allele Dmel\htl^Scer\UAS.cMa