Co-expression of htlScer\UAS.cMa rescues the phenotype of reduced bouton number per muscle at the neuromuscular junction of Scer\GAL4how-24B>SmndsRNA.N.Scer\UAS.WIZ or Scer\GAL4how-24B>SmndsRNA.C.Scer\UAS.WIZ third instar larvae.
Expression of htlScer\UAS.cMa under the control of Scer\GAL4alrm.PD strongly restores infiltration of astrocyte processes into the neuropil in htlAB42 embryos, however, migration of the ventral-most astrocyte is not rescued.
Expression of htlScer\UAS.cMa under the control of Scer\GAL4htl.POS restores dorsal muscle development in approximately half of htlAB42 embryos. Some of the rescued embryos develop cardiac and pericardial cells.
Expression of htlScer\UAS.cMa under the control of Scer\GAL4tey-5053A rescues the longitudinal visceral muscle (LVM) founder cell death seen in htlAB42 mutant stage 13 embryos. The founder cell migration defects are also partially rescued, however some cells acquire abnormal shapes, adhere to positions more distant from the TVM both dorsally and ventrally, and form clusters, resulting in missing LVM cells in the most anterior trunk segments.
Expression of htlScer\UAS.cMa under the control of Scer\GAL4twi.PG is able to induce bilaterally symmetrical flattening of the mesodermal tube onto the ectoderm in the early stages of mesoderm morphogenesis in htlAB42 embryos.
htlScer\UAS.cMa; Scer\GAL4twi.PB rescues the migration of the mesoderm after invagination in htlAB42 embryos. As a result the number of hemisegments with eve expressing mesodermal cells is 22, just as in wild-type.
The addition of htlScer\UAS.cMa driven by Scer\GAL4twi.PB fails to rescue the lethality seen in htlYY262/htlAB42. However, when htlScer\UAS.cMa is driven by Scer\GAL4Mz1277, Scer\GAL4Bx-MS1096 and Scer\GAL4twi.PB concurrently, some escaper pupae do survive.