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General Information
Symbol
Dmel\htlUAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Michelson
FlyBase ID
FBal0090412
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Htl, UAS-htlWT
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Full length htl is expressed under the control of UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of htlScer\UAS.cMa driven by Scer\GAL4how-24B does not significantly affect the number of boutons per muscle at the neuromuscular junction (NMJ) of third instar larvae, compared to controls.

Evoked excitatory junction potential (EJP) and miniature EJP (mEJP) amplitude are significantly decreased (there is no significant difference in quantal content or mEJP frequency) at the NMJ of Scer\GAL4how-24B>htlScer\UAS.cMa third instar larvae, compared to controls.

Expression of htlScer\UAS.cMa in developing myoblasts under the control of Scer\GAL41151 results in an increase in the number of myoblasts (specifically tilm myoblasts). Consistent with these larval phenotypes, over-expression leads to a significantly higher number of fibres contributing to leg muscles. For example, the average number of htlScer\UAS.cMa-induced muscle fibres (counted in 10 adult legs) of the tarsus levator muscle (talm) is 14.8 compared to 10 fibres in the wild-type. However, the overall leg muscle pattern and capacity of fibres to interact with internal tendons appears unchanged.

Expression of htlScer\UAS.cMa under the control of Scer\GAL4dpp.blk1 has no effect on furrow formation in the eye disc.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of htlScer\UAS.cMa rescues the phenotype of reduced bouton number per muscle at the neuromuscular junction of Scer\GAL4how-24B>SmndsRNA.N.Scer\UAS.WIZ or Scer\GAL4how-24B>SmndsRNA.C.Scer\UAS.WIZ third instar larvae.

Mesoderm spreading and differentiation in pbl3 homozygous embryos is not rescued by htlScer\UAS.cMa; Scer\GAL4twi.PB.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of htlScer\UAS.cMa under the control of Scer\GAL4alrm.PD strongly restores infiltration of astrocyte processes into the neuropil in htlAB42 embryos, however, migration of the ventral-most astrocyte is not rescued.

Expression of htlScer\UAS.cMa under the control of Scer\GAL4htl.POS restores dorsal muscle development in approximately half of htlAB42 embryos. Some of the rescued embryos develop cardiac and pericardial cells.

Expression of htlScer\UAS.cMa under the control of Scer\GAL4tey-5053A rescues the longitudinal visceral muscle (LVM) founder cell death seen in htlAB42 mutant stage 13 embryos. The founder cell migration defects are also partially rescued, however some cells acquire abnormal shapes, adhere to positions more distant from the TVM both dorsally and ventrally, and form clusters, resulting in missing LVM cells in the most anterior trunk segments.

Expression of htlScer\UAS.cMa under the control of Scer\GAL4twi.PG is able to induce bilaterally symmetrical flattening of the mesodermal tube onto the ectoderm in the early stages of mesoderm morphogenesis in htlAB42 embryos.

htlScer\UAS.cMa; Scer\GAL4twi.PB rescues the migration of the mesoderm after invagination in htlAB42 embryos. As a result the number of hemisegments with eve expressing mesodermal cells is 22, just as in wild-type.

Expression of htlScer\UAS.cMa under the control of Scer\GAL4twi.PG rescues the formation of the visceral mesoderm and salivary gland migration in htlAB42/htlAB42 embryos.

The addition of htlScer\UAS.cMa driven by Scer\GAL4twi.PB fails to rescue the lethality seen in htlYY262/htlAB42. However, when htlScer\UAS.cMa is driven by Scer\GAL4Mz1277, Scer\GAL4Bx-MS1096 and Scer\GAL4twi.PB concurrently, some escaper pupae do survive.

The addition of htlScer\UAS.cMa driven by Scer\GAL4twi.PB, fails rescue the lethality seen in htlYY262/htlAB42. However, when htlScer\UAS.cMa is driven by Scer\GAL4Mz1277, Scer\GAL4Bx-MS1096 and Scer\GAL4twi.PB concurrently some escaper pupae do survive.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
htlScer\UAS.cMa
htlUAS.cMa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Michelson
Secondary FlyBase IDs
    References (15)