|Name||Saccharomyces cerevisiae UAS construct a of Michelson||FlyBase ID||FBal0090412|
|Feature type||allele||Associated gene||Dmel\htl|
|Mutagen||in vitro construct - regulatory fusion|
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
axon & mechanosensory neuron & adult head, with Scer\GAL4Bx-MS1096, Scer\GAL4Mz1277, Scer\GAL4twi.PB, htlYY262/htlAB42
axon & ocellus sensory structure, with Scer\GAL4Bx-MS1096, Scer\GAL4Mz1277, Scer\GAL4twi.PB, htlYY262/htlAB42
Expression of htl[Scer\UAS.cMa] in developing myoblasts under the control of Scer\GAL4 results in an increase in the number of myoblasts (specifically tilm myoblasts). Consistent with these larval phenotypes, over-expression leads to a significantly higher number of fibres contributing to leg muscles. For example, the average number of htl[Scer\UAS.cMa]-induced muscle fibres (counted in 10 adult legs) of the tarsus levator muscle (talm) is 14.8 compared to 10 fibres in the wild-type. However, the overall leg muscle pattern and capacity of fibres to interact with internal tendons appears unchanged.
|Phenotype Manifest In|
|NOT Suppressor of|
|Complementation & Rescue Data|
htlScer\UAS.cMa, Scer\GAL4twi.PB, Scer\GAL4Mz1277, Scer\GAL4Bx-MS1096 partially rescues htlYY262/htlAB42
|Fails to rescue|
Expression of htl[Scer\UAS.cMa] under the control of Scer\GAL4[tey-5053A] rescues the longitudinal visceral muscle (LVM) founder cell death seen in htl[AB42] mutant stage 13 embryos. The founder cell migration defects are also partially rescued, however some cells acquire abnormal shapes, adhere to positions more distant from the TVM both dorsally and ventrally, and form clusters, resulting in missing LVM cells in the most anterior trunk segments.
Expression of htlScer\UAS.cMa under the control of Scer\GAL4twi.PG is able to induce bilaterally symmetrical flattening of the mesodermal tube onto the ectoderm in the early stages of mesoderm morphogenesis in htlAB42 embryos.
htlScer\UAS.cMa; Scer\GAL4twi.PB rescues the migration of the mesoderm after invagination in htlAB42 embryos. As a result the number of hemisegments with eve expressing mesodermal cells is 22, just as in wild-type.
Expression of htlScer\UAS.cMa under the control of Scer\GAL4twi.PG rescues the formation of the visceral mesoderm and salivary gland migration in htlAB42/htlAB42 embryos.
The addition of htlScer\UAS.cMa driven by Scer\GAL4twi.PB fails to rescue the lethality seen in htlYY262/htlAB42. However, when htlScer\UAS.cMa is driven by Scer\GAL4Mz1277, Scer\GAL4Bx-MS1096 and Scer\GAL4twi.PB concurrently, some escaper pupae do survive.
|Stocks ( 2 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Michelson
|Secondary FlyBase IDs|
|References ( 11 )|
|Personal communication to FlyBase|