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General Information
Symbol
Dmel\amnchpd
Species
D. melanogaster
Name
cheapdate
FlyBase ID
FBal0090484
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion of a P{PZ} element in the open reading frame, C-terminal to the PACAP and GHRH homology regions.

Insertion components
P{PZ}amnchpd
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

amnchpd mutants do not exhibit any behavioral memory at 24 hours and only a small amount is present at 9 hours after conditioning. Unlike wild-type, there is no significant increase in odor-evoked calcium influx into the α branch of the mushroom body neurons in amnchpd mutants at either 9 or 24 hours after conditioning.

amnchpd flies have a defective olfactory memory. These flies fail to produce the increased calcium influx seen in the dorsal paired medial neurons of wild-type flies at 30 minutes after a conditioning of 3s exposure to odour, followed by a 60s odour stimulus applied simultaneously with 12 electric-shock pulses.

amnchpd mutant flies show similar response profiles to wild-type flies in response to ethanol: flies have an initial startle response, a brief moment of quiescence followed by a sustained period of hyperactivity and a reduction in locomotor speed leading to eventual immobility. However, the amnchpd mutants have a more intense hyperactive phase and reach maximal hyperactivity more quickly, leading to a faster onset of immobility. During the hyperactive phase, amnchpd mutants initiate activity bouts less often than wild-type flies, but stay active for longer and spend more time moving fast. As these mutants begin to sedate, their bout frequency actually increases but their bout length is shorter.

Hemizygous males and homozygous females show increased sensitivity to ethanol; they elute from an inebriometer with a mean elution time (MET) of approximately 15 minutes compared with an MET of 20 minutes for wild-type controls. amnchpd flies show normal geotaxis and locomotor activity. Ethanol absorption and metabolism is normal in these flies. The ethanol-sensitive phenotype is reversed by treatment of the flies with forskolin (an adenylate cyclase activator) for 2 hours. A 4 hour treatment with forskolin further reduces ethanol sensitivity of amnchpd flies compared with control flies. Treatment of amnchpd flies with a cAMP analog that activates PKA also reverses the ethanol-sensitive phenotype. Treatment of amnchpd flies with an antagonist of cAMP that inhibits PKA partially reverses the ethanol-sensitive phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference

amnchpd is a suppressor of chemical sensitive phenotype of rut2080

Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The sensitivity to ethanol-induced sedation seen in Arf51FNP5226/Arf51FEP2612 flies is enhanced if they also carry amnchpd.

Double heterozygotes for amnchpd and rut1 show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

Double heterozygotes for amnchpd and Pka-C1B10 show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

amnchpd rut2080 double mutants do not show increased sensitivity to ethanol in an inebriometer assay, although each single mutant does show increased sensitivity to ethanol in an inebriometer assay.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The ethanol sensitive phenotype is rescued by amnhs.PM expressed using heat shock. Full rescue of the ethanol-sensitive phenotype is seen in animals given a daily heat shock throughout development, and is still seen 48 hours after the last heat shock, but the phenotype has reverted to ethanol-sensitivity 72 hours after the last heat shock. Three heat shocks at 24 hour intervals in adult flies is also sufficient to fully rescue the ethanol-sensitive phenotype.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Selected as: Altered ethanol sensitivity in an inebriometer assay.

Comments
Comments

Precise excision of the P{PZ} element restores the wild-type phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (12)