Allele Dmel\Med²⁶

General Information
Symbol	Dmel\Med²⁶	Species	D. melanogaster
Name		FlyBase ID	FBal0090587
Feature type	allele	Associated gene	Dmel\Med

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Das et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:27,440,017..27,440,017 comment=Site of nucleic acid difference in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G27440017A pr_change=G609S\|Med-PA,G535S\|Med-PB reported_pr_change=G609S (Das et al., 1998)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: G609S. (Das et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	cell growth defective \| somatic clone (Kirilly et al., 2005)
Phenotype Manifest In
	eye \| somatic clone (Das et al., 1998) female germline stem cell (Xie and Spradling, 1998) follicle cell \| somatic clone (Kirilly et al., 2005) follicle stem cell \| somatic clone (Kirilly et al., 2005) germarium (Jordan et al., 2000) male germline stem cell \| germline clone (Kawase et al., 2004)
Detailed Description
	Statement Reference When neutral marked clones are induced in the ovary, the proportion of germaria carrying marked somatic stem cells 3 weeks after clone induction is around 70% of that seen one week after clone induction. However, for Med²⁶ homozygous clones, the equivalent figure is only 4%. Significantly fewer Med²⁶ homozygous clones are seen in the ovarian follicle cells than neutral control clones, and those clones that are present contain about 50% the number of cells as their wild-type twin-spot clones. The proportion of cells in these clones in S-phase (labelled with BrdU) is also less than in control clones. (Kirilly et al., 2005) Clones of male Med²⁶ homozygous germline stem cells are still present in only 2% of testes one week after clone induction and none are present two weeks after clone induction. This is in contrast to wild-type control clones, which are present in 82% of testes one week after clone induction and 64% two weeks after clone induction. (Kawase et al., 2004) Encapsulation defects of 16-cell cysts are seen in ovaries containing homozygous follicle cell clones. (Jordan et al., 2000) Homozygous clones in the eye are only seen at the margins of the eye, most commonly at the posterior margin, and result in loss of eye tissue. (Das et al., 1998) Clonal analysis in the germarium reveals that mutant stem cells are lost more rapidly than wild type, though there is no effect on the formation of 16 cell cysts or their subsequent development. Stem cell half life is 0.38 weeks (wild type being 4.6 weeks). Stem cell division rate relative to control is 0.39. Cysts contain the normal 16 cells, including and oocyte. (Xie and Spradling, 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Med²⁶/Med[+] is a suppressor of visible phenotype of Scer\GAL4^en-e16E, sax^{Q263D.Scer\UAS.cDa} (Das et al., 1998) Med²⁶/Med[+] is a suppressor of visible phenotype of tkv^SC143 (Das et al., 1998)
Phenotype Manifest In
Suppressor of
Statement Reference Med²⁶/Med[+] is a suppressor of wing phenotype of Scer\GAL4^en-e16E, sax^{Q263D.Scer\UAS.cDa} (Das et al., 1998) Med²⁶/Med[+] is a suppressor of wing phenotype of tkv^SC143 (Das et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference Slightly suppresses the wing phenotype produced by tkv^SC143. (Das et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Med¹ (Das et al., 1998)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Med²⁶ (Kirilly et al., 2005)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Kirilly et al., 2005, Dev. Cell 9(5): 651--662 BMP signaling is required for controlling somatic stem cell self-renewal in the Drosophila ovary. [FBrf0190209] Kawase et al., 2004, Development 131(6): 1365--1375 Gbb/Bmp signaling is essential for maintaining germline stem cells and for repressing bam transcription in the Drosophila testis. [FBrf0174556] Song et al., 2004, Development 131(6): 1353--1364 Bmp signals from niche cells directly repress transcription of a differentiation-promoting gene, bag of marbles, in germline stem cells in the Drosophila ovary. [FBrf0174555] Das et al., 1998, Development 125(8): 1519--1528 The Drosophila gene Medea demonstrates the requirement for different classes of Smads in dpp signaling. [FBrf0102615] Xie and Spradling, 1998, Cell 94(2): 251--260 decapentaplegic is essential for the maintenance and division of germline stem cells in the Drosophila ovary. [FBrf0103396]
Letter	Jordan et al., 2000, Nat. Genet. 24(4): 429--433 The homeobox gene mirror links EGF signalling to embryonic dorso-ventral axis formation through Notch activation. [FBrf0127146]

Allele Dmel\Med26

Allele Dmel\Med²⁶