Allele Hsap\SOD1^Scer\UAS.cPa

General Information
Symbol	Hsap\SOD1^Scer\UAS.cPa	Species	H. sapiens
Name	Saccharomyces cerevisiae UAS construct a of Parkes	FlyBase ID	FBal0090627
Feature type	allele	Associated gene	Hsap\SOD1

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Parkes et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: An Hsap\SOD1 cDNA is expressed under the control of Scer\UAS regulatory sequences. (Parkes et al., 1998)
Carried in construct	P{UAS-Hsap\SOD1.P} (Missirlis et al., 2001, Missirlis et al., 2003, Parkes et al., 1998, Wang et al., 2006, Gruenewald et al., 2009, Martin et al., 2009, Ford et al., 2007, Watson et al., 2008, Kirby et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	long lived, with Scer\GAL4^Act5C.PU (Martin et al., 2009) long lived, with Scer\GAL4^D42 (Parkes et al., 1998) long lived, with Scer\GAL4^da.G32 (Martin et al., 2009)
Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Hsap\SOD1[Scer\UAS.cPa] under the control of Scer\GAL4[Act] does not rescue the reduction in lifespan seen in flies under hyperoxia. Expression of Hsap\SOD1[Scer\UAS.cPa] under the control of Scer\GAL4[ple.PF] rescues the degeneration of dopaminergic neurons that is seen in flies under hyperoxia. (Gruenewald et al., 2009) Expression of Hsap\SOD1[Scer\UAS.cPa] under the control of either Scer\GAL4[Act5C] or Scer\GAL4[da.G32] increases mean life span by 15-30%. Age related impairment in negative geotaxis is modestly delayed in flies expressing Hsap\SOD1[Scer\UAS.cPa] under the control of Scer\GAL4[Act5C] but is not altered in flies expressing Hsap\SOD1[Scer\UAS.cPa] under the control of Scer\GAL4[da.G32]. Expression of Hsap\SOD1[Scer\UAS.cPa] under the control of Scer\GAL4[D42], Scer\GAL4[Appl.G1a], Scer\GAL4[elav-C155], Scer\GAL4[repo], Scer\GAL4[Mef2.PR] or Scer\GAL4[how-24B] does not result in an increase in lifespan. (Martin et al., 2009) Flies expressing Hsap\SOD1[Scer\UAS.cPa] under the control of Scer\GAL4[elav.Switch.PO] in the presence of RU486 show no alteration in lifespan compared to controls. (Ford et al., 2007) Flies expressing Hsap\SOD1^Scer\UAS.cPa under the control of Scer\GAL4^D42 in a wild-type background have mean and maximum adult lifespans up to 40% longer than isogenic controls. The pre-mortality plateau phase of the life curve (less than 5% mortality) is extended from approximately 27 to approximately 50 days. (Parkes et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Hsap\SOD1^Scer\UAS.cPa, Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI is an enhancer of lethal \| recessive phenotype of Trxr-1^G0481 (Missirlis et al., 2001)
Suppressor of
Statement Reference Hsap\SOD1^Scer\UAS.cPa, Scer\GAL4^elav.PLu is a suppressor of neuroanatomy defective phenotype of Pink1^{dsRNA.Scer\UAS.cWa}, Scer\GAL4^elav.PLu (Wang et al., 2006) Hsap\SOD1^Scer\UAS.cPa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Pink1^{dsRNA.Scer\UAS.cWa}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Wang et al., 2006) Hsap\SOD1^Scer\UAS.cPa/Scer\GAL4^D42 is a suppressor \| partially of aging defective \| recessive phenotype of Sod^X-39 (Parkes et al., 1998) Hsap\SOD1^Scer\UAS.cPa/Scer\GAL4^da.G32 is a suppressor of short lived phenotype of Ccs^29E (Kirby et al., 2008) Hsap\SOD1^Scer\UAS.cPa/Scer\GAL4^Mhc.PW is a suppressor of short lived phenotype of Sod^X-39 (Missirlis et al., 2003)
Other
Statement Reference Hsap\SOD1^Scer\UAS.cPa, Scer\GAL4^D42, Sod^X-39 has chemical resistant phenotype (Parkes et al., 1998) Hsap\SOD1^Scer\UAS.cPa, Scer\GAL4^D42, Sod^X-39 has radiation resistant phenotype (Parkes et al., 1998)
Phenotype Manifest In
Suppressor of
Statement Reference Hsap\SOD1^Scer\UAS.cPa, Scer\GAL4^elav.PLu is a suppressor of dopamine neuron & adult brain phenotype of Pink1^{dsRNA.Scer\UAS.cWa}, Scer\GAL4^elav.PLu (Wang et al., 2006) Hsap\SOD1^Scer\UAS.cPa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Pink1^{dsRNA.Scer\UAS.cWa}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Wang et al., 2006) Hsap\SOD1^Scer\UAS.cPa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Pink1^{dsRNA.Scer\UAS.cWa}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Wang et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Hsap\SOD1[Scer\UAS.cPa] under the control of Scer\GAL4[da.G32] rescues the early mortality and reduced median lifespan of Ccs[29E] homozygotes. (Kirby et al., 2008)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Hsap\SOD1^Scer\UAS.cPa Hsap\SOD1^UAS.cPa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Parkes (Parkes et al., 1998)
Secondary FlyBase IDs

References ( 9 )
Research paper	Gruenewald et al., 2009, Free Radical Biol. Med. 46(12): 1668--1676 Hyperoxia-induced neurodegeneration as a tool to identify neuroprotective genes in Drosophila melanogaster. [FBrf0208024] Martin et al., 2009, FEBS Lett. 583(13): 2308--2314 Manipulation of Sod1 expression ubiquitously, but not in the nervous system or muscle, impacts age-related parameters in Drosophila. [FBrf0208254] Kirby et al., 2008, J. Biol. Chem. 283(51): 35393--35401 Instability of superoxide dismutase 1 of Drosophila in mutants deficient for its cognate copper chaperone. [FBrf0207119] Watson et al., 2008, J. Biol. Chem. 283(36): 24972--24981 A drosophila model for amyotrophic lateral sclerosis reveals motor neuron damage by human SOD1. [FBrf0207242] Ford et al., 2007, Exp. Gerontol. 42(6): 483--497 Alteration of Drosophila life span using conditional, tissue-specific expression of transgenes triggered by doxycyline or RU486/Mifepristone. [FBrf0200753] Wang et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(36): 13520--13525 Antioxidants protect PINK1-dependent dopaminergic neurons in Drosophila. [FBrf0192173] Missirlis et al., 2003, J. Biol. Chem. 278(48): 47365--47369 Compartment-specific protection of iron-sulfur proteins by superoxide dismutase. [FBrf0167750] Missirlis et al., 2001, Curr. Biol. 11(16): 1272--1277 Cooperative action of antioxidant defense systems in Drosophila. [FBrf0138264] Parkes et al., 1998, Nat. Genet. 19(2): 171--174 Extension of Drosophila lifespan by overexpression of human SOD1 in motorneurons. [FBrf0102644]

Allele Hsap\SOD1Scer\UAS.cPa

Allele Hsap\SOD1^Scer\UAS.cPa