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General Information
Symbol
Dmel\EgfrUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Buff
FlyBase ID
FBal0090687
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Egfr
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Full length Egfr is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

cortical actin cytoskeleton & oocyte associated follicle cell, with Scer\GAL4slbo.2.6

mesothoracic tarsal segment 1 & bract, with Scer\GAL4sca-537.4

mesothoracic tergum & macrochaeta | supernumerary, with Scer\GAL4ap-md544

taste bristle & leg | ectopic, with Scer\GAL4Dll-md23

taste bristle & leg | ectopic, with Scer\GAL4sca-537.4

Detailed Description
Statement
Reference

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4crq.PO results in significantly increased concentration of circulating hemocytes in third instar larvae, as compared to controls.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4GMR.PU results in small, rough eyes.

Expression of EgfrScer\UAS.cBa in the wing, under the control of Scer\GAL4Bx-MS1096, generates ectopic and defective wing vein formation. Addition of KSOP1009 to the food media suppresses defective wing vein formation.

Third instar larvae expressing EgfrUAS.cBa under the control of Scer\GAL4ap-md544 (in combination with a Gal80[ts] transgene to inhibit expression until early third instar) do not pupate and can continue to grow for up to 5 days at 29[o]C. Their wing discs are severely overgrown in the dorsal compartment with dying cells dropping out of the epithelial layer. Otherwise, apico-basal polarity and epithelial organization in wing discs are similar to wild-type controls.

Somatic clones expressing EgfrUAS.cBa under the control of Scer\GAL4ap-md544 appear to be sorted out and eliminated from the wing disc epithelium in third instar larvae.

Expressing EgfrUAS.cBa under the control of Scer\GAL4hh.PU results in an overgrown wing disc dorsal compartment in third instar larvae.

Neuronal expression of EgfrScer\UAS.cBa (under the control of Scer\GAL4elav-C155) throughout development, until the adult stage (where Scer\GAL80ts.αTub84B is induced) does not reduce ethanol sensitivity. Neuronal expression only after eclosion of the adult fly also fails to reduce ethanol sensitivity.

Border follicle cell clusters in females expressing EgfrScer\UAS.cBa under the control of Scer\GAL4slbo.2.6 show significantly reduced net cluster movement compared to wild type in both early and late phases of posterior migration. Tumbling is increased compared to wild type in both phases. Fewer cellular extensions are observed overall, but the strong bias for extensions from the front of the border cell cluster is retained.

Expression of EgfrScer\UAS.cBa under the control of one of Scer\GAL4elav-C155, Scer\GAL4Ilp2.PR or Scer\GAL4ple.PF results in flies with increased resistance to ethanol-induced sedation compared to control flies.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4GMR.PF results in a very strong rough eye phenotype with prominent blistering in the dorsal anterior section of the eye.

The projection pattern of brain insulin-producing cells appears unaffected in adults expressing EgfrScer\UAS.cBa under the control of Scer\GAL4Ilp2.PR.

The projection pattern of brain ple-positive dopaminergic cells appears unaffected in adults expressing EgfrScer\UAS.cBa under the control of Scer\GAL4ple.PF.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4GMR.PF results in a rough-eye phenotype.

Expression of EgfrScer\UAS.cBa in the wing under the control of Scer\GAL4dpp.blk1 results in elongation of the wing.

Expression of EgfrScer\UAS.cBa in the wing using Scer\GAL4Bx-MS1096 results in wing overgrowth and aberrant wing venation.

Cells expressing EgfrScer\UAS.cBa under the control of Scer\GAL4c522 are preferentially located at the front of the cluster during border cell posterior migration in mosaic border cell clusters consisting of both wild-type and mutant cells.

Overexpression of EgfrScer\UAS.cBa in the developing retina, under the control of Scer\GAL4GMR.PF causes a rough eye.

Overexpression of EgfrScer\UAS.cBa under the control of Scer\GAL4796 increases cell size but does not affect synapse number.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4bi-md653 results in a mild haltere-to-wing transformation as the capitellum of the halteres produce between three and five wing margin-type bristles.

Border cell migration occurs normally in EgfrScer\UAS.cBa; Scer\GAL4slbo.2.6 egg chambers. By oogenesis stage 10, sporadic follicle cells in these egg chambers accumulate filamentous actin in the cortex.

Expression of EgfrScer\UAS.cBa in border cells, driven by Scer\GAL4slbo.2.6 does not affect the formation of the egg chamber apical cap or the migration of the border cell cluster to the oocyte.

When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation is increased and lamellocyte numbers and crystal cells are unaffected.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4sca-537.4 has a very weak effect on a number of notum bristles.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4GMR.PF results in a general disorganisation of the ommatidia.

EgfrScer\UAS.cBa; Scer\GAL4Bx-MS1096 flies have wings with moderate amounts of ectopic vein formation, concentrated around the distal ends of the longitudinal veins. EgfrDN3.Scer\UAS; EgfrScer\UAS.cBa; Scer\GAL4Bx-MS1096 flies have a much more severe wing phenotype: wings are small rounded with large amounts of ectopic vein material.

The expression of EgfrScer\UAS.cBa completely eliminated tibial and basitarsal bracts when driven by Scer\GAL4sca-537.4, and almost completely eliminated them when driven by Scer\GAL4Dll-md23 (None of bracts in the tibia and 10% in the basitarsus of the second leg remain.) Also in 1 in 10 legs (when driven by Scer\GAL4Dll-md23 or Scer\GAL4sca-537.4) a transformation of the huge 'apical' bristle into a chemosensory bristle of ordinary size is seen.

When expression is driven by Scer\GAL4ap-md544 extra macrochatae develop on the notum, but only in the vicinity of extant ones.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4ptc-559.1 results in wings with fusion of veins 3 and 4 in the proximal portion of the wing, while more distally the spacing of veins 3 and 4 appears normal. Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 does not cause formation of ectopic veins between wing veins 3 and 4, although a significant amount of ectopic vein material is induced anterior to vein 3 and posterior to vein 4.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4GMR.PF causes some ectopic wing vein formation.

Expression of EgfrScer\UAS.cBa under the control of both Scer\GAL4twi.PG and Scer\GAL4how-24B in a wild-type background has no effect on muscle development.

Scer\GAL4GMR.PF induced expression causes complete loss of the adult retina, some interommatidial bristles remain.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
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NOT Enhanced by
Suppressed by
Statement
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NOT suppressed by
Enhancer of
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Statement
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Phenotype Manifest In
Enhanced by
Statement
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NOT Enhanced by
Statement
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Suppressed by
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NOT suppressed by
Enhancer of
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NOT Suppressor of
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Additional Comments
Genetic Interactions
Statement
Reference

All individuals co-expressing psqKK111691 and EgfrUAS.cBa under the control of Scer\GAL4ap-md544 during larval stages (using Gal80[ts] for the temporal regulation of expression) are developmentally delayed and die at the third instar stage; their wing discs form epithelial tumors that exhibit cell polarity defects (i.e. Dlg mis-localization), associated with higher numbers of myoblasts and a more extensive/branched trachea than normal across the disc. The tumors metastasize to distant tissues in the larva.

The tumor phenotype (size and disc distortion) and the increased myoblast numbers are partially suppressed by the additional co-expression of either NrgHMS01638, capsID.UAS, SCARGD11380, or diaHM05027, but not of dsHMS00759, fjHMS01310 or ftHMS00932; diaHM05027 also partially suppresses the increased tracheation. There is no further tumor suppression by NrgHMS01638, diaHM05027 combined. Additional co-expression of diadsRNA.lexAop under the control of Ecol\lexAGMR15B03 also partially suppresses the tumors and the increased myoblasts.

Co-expression of diaHM05027 under the control of Scer\GAL4ap-md544 also rescues most Scer\GAL4ap-md544>psqKK111691,EgfrUAS.cBa individuals to adulthood; these adults are fertile and their only visible phenotype is wing blade defects.

The co-expression of EgfrUAS.cBa and pnutGD1512 from the second instar larval stage under the combined control of Scer\GAL4ap-md544 and Gal80[ts] does not leads to the formation of tumors in the third instar larval wing disc.

The increased concentration of circulating hemocytes observed in third instar larvae expressing EgfrScer\UAS.cBa under the control of Scer\GAL4crq.PO is suppressed by co-expression of CblL.Scer\UAS but not CblL.Δ70Z.Scer\UAS.

Expression of psqKK111691 massively enhances the overproliferation seen in wing discs expressing EgfrScer\UAS.cBa under the control of Scer\GAL4ap-md544. The discs grow as multilayered masses. The larvae do not enter pupariation and the discs continue to grow, often filling the anterior half of the larva. The cells lose their apical-basal polarity and cells metastasise into internal organs including the gut and malpighian tubules. The basement membranes have degraded in these discs. Mutant epithelial cells are intermingled with wild type mesenchymal cells that resemble the adepithelial cells. Expression of psqKK111691 and EgfrScer\UAS.cBa specifically in the wing pouch under the control of Scer\GAL4nub.PU produces epithelial hyperplasia but no tumors. The proliferation of both the epithelial and mesenchymal cells in the tumor is rapid, with most of the mesenchymal proliferation taking place close to the epithelial layer.

Expression of psqGL00342 massively enhances the overproliferation seen in wing discs expressing EgfrScer\UAS.cBa under the control of Scer\GAL4ap-md544.

Expression of psqJF03109 massively enhances the overproliferation seen in wing discs expressing EgfrScer\UAS.cBa under the control of Scer\GAL4ap-md544.

Expression of dppJF01371 partially suppresses the growth of the massive wing disc tumors seen when EgfrScer\UAS.cBa and psqKK111691 are expressed under the control of Scer\GAL4ap-md544.

Expression of wgGD5007 partially suppresses the growth of the massive wing disc tumors seen when EgfrScer\UAS.cBa and psqKK111691 are expressed under the control of Scer\GAL4ap-md544.

Expression of trolGL01153 suppresses the growth of the massive wing disc tumors seen when EgfrScer\UAS.cBa and psqKK111691 are expressed under the control of Scer\GAL4ap-md544. Apicobasal polarity and tissue organisation is restored to the epithelial cells.

Expression of trolKK105502 suppresses the growth of the massive wing disc tumors seen when EgfrScer\UAS.cBa and psqKK111691 are expressed under the control of Scer\GAL4ap-md544. Apicobasal polarity and tissue organisation is restored to the epithelial cells.

Co-expression of trolEP1160 and EgfrScer\UAS.cBa in the wing disc under the control of Scer\GAL4ap-md544 results in massive overgrowth and neoplastic transformation. Mutant epithelial cells intermingle with wild type mesenchymal cells.

Ablating adepithelial cells through expression of rprEcol\lexAop.cHa under the control of Ecol\lexAGMR15B03 suppresses the neoplastic transformation seen when psqKK111691 and EgfrScer\UAS.cBa are co-expressed under the control of Scer\GAL4ap-md544.

Expression of either wgScer\UAS.cLa or dppScer\UAS.cSa does not enhance the overproliferation seen in wing discs expressing EgfrScer\UAS.cBa under the control of Scer\GAL4ap-md544. However, co-expression of wgScer\UAS.cLa and dppScer\UAS.cSa with EgfrScer\UAS.cBa produces tumors consisting of epithelial and mesenchymal cell populations and showing loss of epithelial integrity.

Expression of MadKK109050 does not suppress the growth of the massive wing disc tumors seen when EgfrScer\UAS.cBa and psqKK111691 are expressed under the control of Scer\GAL4ap-md544. Apicobasal polarity and tissue organisation is restored to the epithelial cells.

Expression of MaddsRNA.IVS.13xEcol\lexAop in adepithelial cells under the control of Ecol\lexAGMR15B03 suppresses the neoplastic transformation seen when psqKK111691 and EgfrScer\UAS.cBa are co-expressed under the control of Scer\GAL4ap-md544.

Expression of panΔN.Scer\UAS suppresses the growth of the massive wing disc tumors seen when EgfrScer\UAS.cBa and psqKK111691 are expressed under the control of Scer\GAL4ap-md544. Apicobasal polarity and tissue organisation is restored to the epithelial cells.

Expression of FakScer\UAS.T:Ivir\HA1 suppresses the reduction in eye size seen in flies expressing EgfrScer\UAS.cBa under the control of Scer\GAL4GMR.PU.

Expression of FakScer\UAS.T:Ivir\HA1 partially suppresses the almost total lethality seen when EgfrScer\UAS.cBa is expressed under the control of Scer\GAL4ptc-559.1. The proportion of surviving flies is increased to approximately 60%.

Co-expressing EgfrUAS.cBa with either mir-banC.UAS.EGFP or Socs36EGD8929 under the control of Scer\GAL4ap-md544 (in combination with a Gal80[ts] transgene to inhibit expression until early third instar) or expression of EgfrUAS.cBa under the control of Scer\GAL4hh.PU in a Socs36EEY11/Socs36EEY11 background result in severely overgrown and deformed wing discs with a disrupted epithelial organization (resembling tissues undergoing a neoplastic transformation) in third instar larvae. Wing discs co-expressing EgfrUAS.cBa and Socs36EGD8929 lose apico-basal polarity and cells metastasize invading other tissues of the larva. Transplantation of imaginal disc fragments co-expressing EgfrUAS.cBa and Socs36EGD8929 under the control of Scer\GAL4ap-md544 into the abdomens of adult hosts form tumors, grow rapidly, metastasize and kill the host flies.

Somatic clones co-expressing EgfrUAS.cBa and Socs36EGD8929 under the control of Scer\GAL4ap-md544 in wing discs of third instar larvae overgrow, fuse and affect the shape of the disc.

Expression of EgfrScer\UAS.cBa enhances the rough eye phenotype seen when Dcp-1Scer\UAS.cKa (insertion line P{UAS-Dcp-1.K}19-2) is expressed under the control of Scer\GAL4GMR.PF.

Cardiac-specific expression of EgfrScer\UAS.cBa driven by Scer\GAL4tin.CΔ4 in Df(3L)ED4238/+ mutants restores normal cardiac function.

Co-expression of hppyRB.Scer\UAS suppresses the rough eye and blistering phenotypes caused by expression of EgfrScer\UAS.cBa under the control of Scer\GAL4GMR.PF.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4GMR.PF results in a rough-eye phenotype, which is enhanced by the presence of Fas2EB112/+.

Co-expression of picoIR.Scer\UAS suppresses the wing overgrowth seen when EgfrScer\UAS.cBa is expressed in the wing using Scer\GAL4Bx-MS1096.

A heterozygous auxI670K/+ background has no effect on the rough eye phenotype observed in EgfrScer\UAS.cBa;Scer\GAL4GMR.PF mutants.

Coexpression of vnScer\UAS.cSa and EgfrScer\UAS.cBa under the control of Scer\GAL4bi-md653 results in an enhancement of the haltere-to-wing transformation see when either transgene is expressed alone. This is an enhancement rather than an additive effect as the capitellum of the haltere produces between 10 and 22 bristles in the double transgene-expressing flies vs 5 at the most in the single transgene flies.

Expression of EgfrScer\UAS.cBa results in an enhancement of the haltere-to-wing transformation of Ubxlac1-GAL4/+ mutants as the capitellum of the halteres produce 40-45 wing margin-type bristles in EgfrScer\UAS.cBa; Ubxlac1-GAL4/+ flies while there are only 4-7 bristles in Ubxlac1-GAL4/+ flies. The halteres of Ubxlac1-GAL4/+ flies expressing EgfrScer\UAS.cBa also show increased pigmentation compared to Ubxlac1-GAL4/+ or wild-type flies. Coexpression of vnScer\UAS.cSa and EgfrScer\UAS.cBa in a Ubxlac1-GAL4/+ background results in a further enhancement of the phenotype.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4bi-md653 in an argosunspecified/+ background results in an enhancement of the haltere-to-wing transformation seen when EgfrScer\UAS.cBa is expressed in a wild-type background. Scer\GAL4bi-md653>EgfrScer\UAS.cBa; argosunspecified/+ flies produce 9-12 wing margin-type bristles on the capitellum of the haltere compared to 3-5 bristles produced by Scer\GAL4bi-md653>EgfrScer\UAS.cBa flies.

Expression of EgfrScer\UAS.cBa under the control of Scer\GAL4bi-md653 in a Ubx1/+ background results in a marginal enhancement of the wing-to-haltere transformations seen in Ubx1/+ mutants, or flies expressing EgfrScer\UAS.cBa in a wild-type background.

Defective migration of border follicle cells in CblF165 homozygous somatic clones is enhanced by EgfrScer\UAS.cBa with Scer\GAL4slbo.2.6.

Migration of border follicle cells is normal in spri6G1 homozygotes, or in flies carrying EgfrScer\UAS.cBa with Scer\GAL4slbo.2.6, but significant defects in border cell migration, including some instances of complete failure of migration are seen in spri6G1/spri6G1 EgfrScer\UAS.cBa animals.

Co-expression of edΔECD.Scer\UAS and EgfrScer\UAS.cBa under the control of Scer\GAL4sca-537.4 results in severe tufting of bristles on the notum.

Co-expression of EgfrScer\UAS.cBa and foxoScer\UAS.cKa under the control of Scer\GAL4GMR.PF does not rescue the eye phenotype seen in animals expressing foxoScer\UAS.cKa alone under the control of Scer\GAL4GMR.PF (ommatidia and bristles are still missing) and the general disorganisation of the ommatidia seen in animals expressing EgfrScer\UAS.cBa alone under the control of Scer\GAL4GMR.PF is also unaffected in the double mutant flies.

Expression of both CblDv.Scer\UAS and EgfrScer\UAS.cBa under the control of Scer\GAL4GMR.PF significantly enhances both the rough eye and extent of ectopic wing veins.

Co-expression of EgfrDN.Scer\UAS.cBa, EgfrScer\UAS.cBa and rhoScer\UAS.cBa driven by Scer\GAL4Bx-MS1096 fully restores the rhoScer\UAS.cBa induced ectopic vein phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
EgfrScer\UAS.cBa
EgfrScer\UAS.cMa
EgfrUAS.cBa
EgfrUAS.cMa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Buff
Secondary FlyBase IDs
  • FBal0058985
References (41)