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General Information
Symbol
Dmel\E2f1UAS.cNa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Neufeld
FlyBase ID
FBal0090700
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-de2f1, UAS-E2F, UAS-E2F1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
A 4.4kb E2f1 cDNA is expressed under the control of UASt regulatory sequences.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of E2fScer\UAS.cNa under the control of Scer\GAL4Act88F.PD results in wings which are gnarled, downward-curved and/or blistered.
Scer\GAL4Act88F.PD, E2fScer\UAS.cNa flies display multiple wing defects.
Expression of E2fScer\UAS.cNa under the control of Scer\GAL4Act88F.PD results in an gnarled wing phenotype.
Scer\GAL4Act88F.PD-driven expression of E2fScer\UAS.cNa causes punctate pattern of cell cell death in the distal wing blade of newly eclosed adults. Scer\GAL4GMR.PF driven expression of E2fScer\UAS.cNa causes a blackened and rough eye phenotype.
Ectopic expression of E2fScer\UAS.cNa under the regulation of Scer\GAL4sca-537.4 results in an increase in cell number in some abdominal hemisegments. In addition transformation of NB6-4a to NB6-4t is observed at low penetrance (13%).
The average cell doubling time in wing disc cells expressing E2fScer\UAS.cNa under the control of Scer\GAL4Act5C.PP is shortened to 9.5 hours (compared to 12 hours for wild-type cells). The average duration of cell cycle phases in these cells is: G1 = 1.4 hours, S = 4.6 hours and G2 = 3.5 hours (wild-type values are G1 = 3.4 hours, S = 4.4 hours and G2 = 4.2 hours). The number of posterior cells in wing discs expressing E2fScer\UAS.cNa under the control of Scer\GAL4en-e16E is higher than that of wild-type discs.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, enhanceable by Asx[+]/Asx1
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, enhanceable by pho[+]/pho1
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, enhanceable by Su(z)21.b7/Su(z)2[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, enhanceable by Su(z)2k06344/Su(z)2[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, enhanceable by tou2/tou[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, enhanceable by His2Av[+]/His2Av810
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, enhanceable by His2Av[+]/His2Av05146
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, enhanceable by Pc3/Pc[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, enhanceable by Pc[+]/Pcf01890
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, enhanceable by Su(z)21/Su(z)2[+]
NOT Enhanced by
Statement
Reference
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-enhanceable by eff[+]/effmer4
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-enhanceable by e(Pc)84DET66.1/e(Pc)84DE[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-enhanceable by kisBG01657/kis[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-enhanceable by Su(z)21.a1/Su(z)2[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-enhanceable by Su(z)123/Su(z)12[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-enhanceable by brm[+]/brm2
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-enhanceable by trx[+]/trxKG08639
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by Asx[+]/Asx1
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by eff[+]/effmer4
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by e(Pc)84DET66.1/e(Pc)84DE[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by kisBG01657/kis[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by pho[+]/pho1
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by Su(z)21.a1/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by Su(z)21.b7/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by Su(z)2k06344/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by Su(z)123/Su(z)12[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by tara1/tara[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by tou2/tou[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by brm[+]/brm2
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by trx[+]/trxKG08639
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-enhanceable by Mi-2[+]/Mi-2j3D4
Suppressed by
Statement
Reference
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, suppressible | partially by E(Pc)[+]/E(Pc)w3
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, suppressible | partially by E(Pc)D4/E(Pc)[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, suppressible | partially by Mi-2[+]/Mi-2j3D4
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, suppressible | partially by Su(z)2[+]/Su(z)23-1
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, suppressible by DarkCD4/Ark[+]
NOT suppressed by
Statement
Reference
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-suppressible by eff[+]/effmer4
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-suppressible by e(Pc)84DET66.1/e(Pc)84DE[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-suppressible by kisBG01657/kis[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-suppressible by Su(z)21.a1/Su(z)2[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-suppressible by Su(z)123/Su(z)12[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-suppressible by brm[+]/brm2
E2f1UAS.cNa, Scer\GAL4Act88F.PD has visible phenotype, non-suppressible by trx[+]/trxKG08639
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by Asx[+]/Asx1
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by eff[+]/effmer4
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by e(Pc)84DET66.1/e(Pc)84DE[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by kisBG01657/kis[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by pho[+]/pho1
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by Su(z)21.a1/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by Su(z)21.b7/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by Su(z)2k06344/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by Su(z)123/Su(z)12[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by tara1/tara[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by tou2/tou[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by brm[+]/brm2
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by trx[+]/trxKG08639
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has visible phenotype, non-suppressible by Mi-2[+]/Mi-2j3D4
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, enhanceable by Asx[+]/Asx1
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, enhanceable by pho[+]/pho1
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, enhanceable by Su(z)21.b7/Su(z)2[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, enhanceable by Su(z)2k06344/Su(z)2[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, enhanceable by tou2/tou[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, enhanceable by His2Av[+]/His2Av810
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, enhanceable by His2Av[+]/His2Av05146
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, enhanceable by Pc3/Pc[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, enhanceable by Pc[+]/Pcf01890
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, enhanceable by Su(z)21/Su(z)2[+]
NOT Enhanced by
Statement
Reference
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-enhanceable by eff[+]/effmer4
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-enhanceable by e(Pc)84DET66.1/e(Pc)84DE[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-enhanceable by kisBG01657/kis[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-enhanceable by Su(z)21.a1/Su(z)2[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-enhanceable by Su(z)123/Su(z)12[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-enhanceable by brm[+]/brm2
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-enhanceable by trx[+]/trxKG08639
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by Asx[+]/Asx1
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by eff[+]/effmer4
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by e(Pc)84DET66.1/e(Pc)84DE[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by kisBG01657/kis[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by pho[+]/pho1
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by Su(z)21.a1/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by Su(z)21.b7/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by Su(z)2k06344/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by Su(z)123/Su(z)12[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by tara1/tara[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by tou2/tou[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by brm[+]/brm2
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by trx[+]/trxKG08639
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-enhanceable by Mi-2[+]/Mi-2j3D4
Suppressed by
Statement
Reference
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, suppressible | partially by E(Pc)[+]/E(Pc)w3
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, suppressible | partially by E(Pc)D4/E(Pc)[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, suppressible | partially by Mi-2[+]/Mi-2j3D4
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, suppressible | partially by Su(z)2[+]/Su(z)23-1
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing blade phenotype, suppressible by DarkCD4/Ark[+]
NOT suppressed by
Statement
Reference
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-suppressible by eff[+]/effmer4
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-suppressible by e(Pc)84DET66.1/e(Pc)84DE[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-suppressible by kisBG01657/kis[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-suppressible by Su(z)21.a1/Su(z)2[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-suppressible by Su(z)123/Su(z)12[+]
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-suppressible by brm[+]/brm2
E2f1UAS.cNa, Scer\GAL4Act88F.PD has wing phenotype, non-suppressible by trx[+]/trxKG08639
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by Asx[+]/Asx1
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by eff[+]/effmer4
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by e(Pc)84DET66.1/e(Pc)84DE[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by kisBG01657/kis[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by pho[+]/pho1
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by Su(z)21.a1/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by Su(z)21.b7/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by Su(z)2k06344/Su(z)2[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by Su(z)123/Su(z)12[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by tara1/tara[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by tou2/tou[+]
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by brm[+]/brm2
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by trx[+]/trxKG08639
DpUAS.cDa, E2f1UAS.cNa, Scer\GAL4GMR.PU has eye phenotype, non-suppressible by Mi-2[+]/Mi-2j3D4
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Expression of mir-11Scer\UAS.T:Disc\RFP-DsRed2 suppresses the apoptosis seen in the wings of newly eclosed flies expressing E2f1Scer\UAS.cNa under the control of Scer\GAL4Act88F.PD. Expression of mir-998Scer\UAS.cTa does not suppress the apoptosis seen in the wings of newly eclosed flies expressing E2f1Scer\UAS.cNa under the control of Scer\GAL4Act88F.PD. Expression of mir-11Scer\UAS.T:Disc\RFP-DsRed2 suppresses the apoptosis seen in the eye disc in flies expressing E2f1Scer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PU. Expression of mir-998Scer\UAS.cTa does not suppress the apoptosis seen in the eye disc in flies expressing expressing E2f1Scer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PU.
Cells posterior to the second mitotic wave in the eye disc which are co-expressing E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF undergo ectopic S phases. In addition, independently of the ectopic S phases, co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF results in the induction of apoptosis in the eye disc. The addition of mir-11Scer\UAS.T:Disc\RFP-DsRed2 does not prevent the ectopic S phases which are induced posterior to the morphogenetic furrow by co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF. However, co-expression of mir-11Scer\UAS.T:Disc\RFP-DsRed2 does suppress the ability of co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF to induce apoptosis in the eye disc. Co-expression of mir-11Scer\UAS.T:Disc\RFP-DsRed2 strongly suppresses the wing defects caused by expression of E2fScer\UAS.cNa under the control of Scer\GAL4Act88F.PD.
Scer\GAL4tub-driven co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa in follicle stem cell (FSC) clones suppresses the CycEWX-associated defective FSC-maintenance phenotype. Scer\GAL4tub- and Scer\GAL4Act5C.PI-driven co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa in CycEAR95 follicle stem cell (FSC) clones also expressing CycEWX.Scer\UAS.cWa suppresses the CycEAR95-associated defective FSC-maintenance phenotype.
Overexpression of E2fScer\UAS.cNa and DpScer\UAS.cDa in a stripe of cells in the wing using Scer\GAL4ptc-559.1 results in high levels of cell death. Co-expression of picoScer\UAS.T:Zzzz\His6,T:Hsap\MYC with E2fScer\UAS.cNa and DpScer\UAS.cDa in a stripe of cells in the wing using Scer\GAL4ptc-559.1 fails to suppress cell death.
The wing phenotype of Scer\GAL4Act88F.PD, E2fScer\UAS.cNa is suppressed by co-expression of RbfScer\UAS.cDa. The wing phenotype of Scer\GAL4Act88F.PD, E2fScer\UAS.cNa is unaffected by co-expression of p53259H.GUS. The rough eye phenotype of Scer\GAL4GMR.PF, E2fScer\UAS.cNa, DpScer\UAS.cDa flies is unaffected by co-expression of p53259H.GUS. Scer\GAL4GMR.PF drives the expression of E2fScer\UAS.cNa and DpScer\UAS.cDa in the posterior region of the eye disc inducing a pattern of apoptosis. The apoptosis in the eye disc induced by the co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF is unaffected by homozygous p535A-1-4.
Co-expression of armS2.Scer\UAS.T:Hsap\MYC strongly suppresses the gnarled wing phenotype caused by expression of E2fScer\UAS.cNa under the control of Scer\GAL4Act88F.PD. Co-expression of panScer\UAS.cWa partially suppresses the gnarled wing phenotype caused by expression of E2fScer\UAS.cNa under the control of Scer\GAL4Act88F.PD. Co-expression of sggScer\UAS.cBa enhances the gnarled wing phenotype caused by expression of E2fScer\UAS.cNa under the control of Scer\GAL4Act88F.PD. Simultaneous expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PU partially suppresses the rough eye phenotype caused by armS44Y.GMR.
Expression of DpScer\UAS.cDa and E2fScer\UAS.cNa in the brain under the control of Scer\GAL4elav.PLu results in lethality. 10 day old brains from animals expressing DpScer\UAS.cDa and E2fScer\UAS.cNa under the control of the selective driver Scer\GAL4elav.Switch.PO display marked loss of cortical neurons and rarefaction of the underlying neuropil compared to controls.
The blackened and rough eye phenotype resulting from the expression of Scer\GAL4GMR.PF-driven E2fScer\UAS.cNa is enhanced by co-expression of Aac11dsRNA.Scer\UAS.
Co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF produces significant excess mitoses in the eye disc. Co-expression of DlScer\UAS.cJa results in a moderate but reproducible and statistically significant increase in the number of mitoses seen in eye discs expressing E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF. Co-overexpression of CycAScer\UAS.cWa, E2fScer\UAS.cNa and DpScer\UAS.cDa (using Scer\GAL4GMR.PF) does not significantly rescue the S phase phenotype seen in DlRevF10 clones in a Minute/+ background.
When E2fScer\UAS.cNa and DpScer\UAS.cDa are driven by Scer\GAL4ptc-559.1 cells in the wing disc overproliferate. Many of these cells drop out of the epithelial layer and have pycnotic nuclei. The addition of banEP3622 enhances the overproliferation phenotype seen in E2fScer\UAS.cNa, DpScer\UAS.cDa, Scer\GAL4ptc-559.1 animals. Fewer cells show pyknotic nuclei, though many cells still drop out of the epithelial layer.
Marked clones in the eye disc which are homozygous for scrib1 and are co-expressing DpScer\UAS.cDa, E2fScer\UAS.cNa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act5C.PI do not show metastatic behaviour.
Co-expression of both E2f1Scer\UAS.cNa and DpScer\UAS.cDa in starved larvae under the control of Scer\GAL4Act5C.PP is sufficient to drive most endoreplicating cells into S phase, as assayed by BrdU incorporation.
Co-expression of DpScer\UAS.cDa enhances the effects of E2fScer\UAS.cNa expression.
Xenogenetic Interactions
Statement
Reference
Co-expression of BacA\p35Scer\UAS.cUa suppresses the induction of apoptosis in the eye disc caused by co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF.
Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 results in a wing nicking phenotype, which is partially suppressed by vgnull/+. Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 rescues wing appendage growth in vg83b27 flies; wing blade features such as bristles, wing veins and margin are often seen in the rescued appendages. Co-expression of E2fScer\UAS.cNa, DpScer\UAS.cDa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4vg.int2.1 does not wing blade growth in vgnull flies and only a slight increase in wing hinge growth is seen.
Expression of E2fScer\UAS.cNa and BacA\p35Scer\UAS.cHa under the control of Scer\GAL4ptc-559.1 in vgnull mutant discs results in an increase in the number of cells in S-phase.
Complementation and Rescue Data
Comments
Co-expression of E2fScer\UAS.cNa rescues the rough eye phenotype caused by expression of E2fdsRNA.Scer\UAS.cJa under the control of Scer\GAL4GMR.PU.
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Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
E2f1Scer\UAS.cNa
E2f1UAS.cNa
E2fScer\UAS.cNa
E2fUAS.cNa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Neufeld
Secondary FlyBase IDs
    References (32)