Allele Dmel\Clk^Jrk

General Information
Symbol	Dmel\Clk^Jrk	Species	D. melanogaster
Name	Clock^Jrk	FlyBase ID	FBal0090722
Feature type	allele	Associated gene	Dmel\Clk
Also Known As	dClk^Jrk, Jrk

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Allada et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:7,758,059..7,758,059 evidence=experimental pr_change=Q714\|Clk-PF,Q780\|Clk-PD,Q780\|Clk-PC,Q776\|C lk-PA,Q776@\|Clk-PB reported_na_change=C?T reported_pr_change=Q776@ na_change=C7758059T (Allada et al., 1998)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: C to T. Amino acid replacement: Q776@. (Allada et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective (Yoshii et al., 2005) circadian rhythm defective (Ceriani et al., 2002, Xu et al., 2008) eclosion rhythm defective (Allada et al., 1998) locomotor rhythm defective (Allada et al., 2003, Kim et al., 2002, Hung et al., 2009) locomotor rhythm defective (with Clk^ar) (Allada et al., 2003) locomotor rhythm defective (with Clk^Z3-0084) (Wu et al., 2008) locomotor rhythm defective \| dominant (Wu et al., 2008) locomotor rhythm defective \| semidominant (Allada et al., 1998) visual behavior defective (Mazzoni et al., 2005)
Phenotype Manifest In
	larval LN period neuron (Park et al., 2000)
Detailed Description
	Statement Reference 1 day memory retention is normal after spaced training in an olfactory conditioning assay. (Chen et al., 2012) Triglyceride levels in Clk[Jrk] mutants are similar to wild-type. (Diangelo et al., 2011) Mutant flies show no rhythmic locomotor activity under free-running conditions (after entrainment in light-dark cycles or temperature cycles). (Hung et al., 2009) 44% of heterozygous flies are arrhythmic under constant darkness conditions. 100% of Clk[Z3-0084]/Clk[Jrk] flies are arrhythmic under constant darkness conditions. (Wu et al., 2008) Clk[Jrk] mutants consume similar amounts of food, regardless of the circadian period (i.e. light or dark) after 3 days of entrainment to LD and then transference to DD for 2 days before the assay. Clk[Jrk] flies display rhythmic feeding patterns in LD, indicating that the feeding rhythm is under the control of an internal clock, but can also be driven by light. Clk[Jrk] mutants do not display phenotypes related to either survival time under starvation conditions or glycogen storage. Total food consumption is also unchanged in these mutants compared to controls. (Xu et al., 2008) Clk^Jrk has no effect on experience-dependent responses in sleep need. (Ganguly-Fitzgerald et al., 2006) Clk[Jrk] mutant larvae are highly sensitive to light (shown in light avoidance assays), even at low intensities (150 lux). (Mazzoni et al., 2005) Mutant flies are arrhythmic for locomotor activity under constant light conditions at a constant temperature of 25[o]C, but become rhythmic immediately after being exposed to a temperature cycle of 12 hours at 25[o]C and 12 hours at 30[o]C. The locomotor activity is rather dispersed over 24 hours with slightly higher activity during the thermophase. The locomotor activity rhythm of the mutant flies differs from that of wild-type flies, in that neither anticipatory activity nor transients after a transfer to temperature cycles is observed. (Yoshii et al., 2005) There is no significant increase in the time spent copulating by pairs of Clk^Jrk homozygous flies compared to wild-type pairs. (Beaver and Giebultowicz, 2004) Long term memory of courtship conditioning (reduction in time spent in courtship behaviour 5 days after a 7 hour conditioning) is normal in Clk^Jrk homozygous males. (Sakai et al., 2004) Heterozygotes exhibit a locomotor rhythmicity of ~24.67 hours. Homozygotes are arrhythmic. Clk^Jrk/Clk^ar animals are arrhythmic. (Allada et al., 2003) Clk^Jrk mutant females mated to Clk^Jrk mutant males lay significantly less eggs and produce significantly less progeny than wild-type flies. The percentage of unfertilised eggs from this mating is not significantly greater than for wild-type. (Beaver et al., 2002) Clk^Jrk flies are often arrhythmic; only one third of these mutants are rhythmic in LD and over half are completely arrhythmic in DD. (Ceriani et al., 2002) The startle response to lights-on normally seen in the locomotor activity rhythms of wild-type flies exposed to 12 hour light:12 hour dark (12:12LD) conditions is not seen in Clk^Jrk flies. The flies are preferentially nocturnal. Under constant darkness conditions, Clk^Jrk flies show roughly equal amounts of locomotor activity during the subjective day and night. (Kim et al., 2002) Mutant females show a more prominent sleep rebound after sleep deprivation (in constant darkness) than wild-type flies; they recover 100% of lost sleep within 12 hours (wild-type flies recover 30-40% of lost sleep within 12 hours). Exposure of mutant females to heat before 12 hours of sleep deprivation reduces homeostatic drive (sleep rebound). (Shaw et al., 2002) In wild-type larvae, dorsally projecting axonal processes arising from the s-LN_v cells terminate near the calyx of the dorsal brain mushroom body. These projections are absent in Clk^Jrk brains. The projections from the l-LN_v cells are also aberrant. Approximately half of mutant brains have abnormal projections; in others one or two axons in this region projected farther and irregularly toward a dorsal or median region of the brain. None of these projections are similar to then more dorsal-reaching projections in the brains of wild-type adults. (Park et al., 2000) Homozygotes show completely arrhythmic locomotor behaviour in constant darkness. 47% of heterozygotes are arrhythmic under constant darkness, and those flies that do show rhythmic locomotor behaviour in these conditions have a slightly longer period than wild-type controls. Only 64% of heterozygotes and 14% of homozygotes show measurable rhythmic locomotor behaviour under 12 hour light:12 hour dark conditions. Like wild-type flies, some homozygotes react to the lights-on transition, but they fail to react to the lights-off transition. Homozygotes are also arrhythmic with respect to adult emergence. (Allada et al., 1998)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	Clk^Jrk is partially rescued by Clk^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^cry.PE (Allada et al., 2003)
Comments
Stocks ( 1 )
Bloomington	24515 Clk^Jrk st¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	Clk^jrk (Kim and Edery, 2006, Ceriani et al., 2002, Shaw and Franken, 2003, Shaw, 2003, Shaw et al., 2002, Sarov-Blat et al., 2000, Wijnen et al., 2006, Wu et al., 2008, Wu et al., 2010) clk^jrk (Michard-Vanhee et al., 2001, Hendricks et al., 2001, Richier et al., 2008, Lamaze et al., 2011) Clk^Jrk (Collins et al., 2006, Lee et al., 2006, Yu et al., 2006, Ganguly-Fitzgerald et al., 2006, Lim et al., 2007, Matsumoto et al., 2007, Mazzoni et al., 2005, Kadener et al., 2007, Diangelo et al., 2011, Hung et al., 2009, Xu et al., 2008, Sehadova et al., 2009, Yoshii et al., 2005, Luo and Sehgal, 2012) Clk^JRK (Fujii et al., 2008) Clock^Jrk (Bahn et al., 2009) dClk^Jrk (Sakai et al., 2004, Ueda et al., 2001, Kloss et al., 2001, Scully and Kay, 2000, Glossop et al., 1999, Hao et al., 1999, Chen et al., 2012) dClk^jrk (Kim et al., 2002, Sun et al., 2010) Jrk (Wang et al., 2001, Ishida et al., 1999, Whitmore et al., 1998, Hardin, 1998, Reppert, 1998, Allada et al., 1998)
Name Synonym	Clock^jrk (Wijnen et al., 2006) Clock^Jrk (Lee et al., 2006, )
Secondary FlyBase IDs

References ( 58 )

Generate a list of	All references relating to this allele ( 58 )

List References by type	Research paper ( 46 ) Review ( 9 ) Abstract ( 3 )
Recent research papers ( 4 )
	Chen et al., 2012, Science 335(6069): 678--685 Visualizing long-term memory formation in two neurons of the Drosophila brain. [FBrf0217453] Luo and Sehgal, 2012, Cell 148(4): 765--779 Regulation of Circadian Behavioral Output via a MicroRNA-JAK/STAT Circuit. [FBrf0217497] Diangelo et al., 2011, PLoS ONE 6(5): e19921 The central clock neurons regulate lipid storage in Drosophila. [FBrf0213791] Lamaze et al., 2011, EMBO Rep. 12(6): 549--557 The E3 ubiquitin ligase CTRIP controls CLOCK levels and PERIOD oscillations in Drosophila. [FBrf0213851] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...

Allele Dmel\ClkJrk

Allele Dmel\Clk^Jrk