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General Information
Symbol
Dmel\tshUAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Gallet
FlyBase ID
FBal0091132
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-tsh, UASTsh
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A full length tsh cDNA is expressed under the control of UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

larval antennal segment & denticle | ectopic, with Scer\GAL469B

larval maxillary segment & denticle | ectopic, with Scer\GAL469B

leg & cuticle | dorsal | somatic clone, with Scer\GAL4Act5C.PP

leg & cuticle | dorsal | somatic clone, with Scer\GAL4VP16.arm

leg & cuticle | somatic clone, with Scer\GAL4Act5C.PP

leg & cuticle | somatic clone, with Scer\GAL4VP16.arm

leg & cuticle | ventral | somatic clone, with Scer\GAL4Act5C.PP

leg & cuticle | ventral | somatic clone, with Scer\GAL4VP16.arm

Detailed Description
Statement
Reference

tshScer\UAS.cGa under the control of Scer\GAL4GMR.PF results in roughening of the posterior portion of the adult eye as a result of nonspecific loss of photoreceptor cells within each ommatidium.

tshScer\UAS.cGa under the control of Scer\GAL4dpp.PS induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs show an increase in size compared to wildtype and have an abnormal profile.

tshScer\UAS.cGa under the control of Scer\GAL4cb26 induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs show an increase in size compared to wildtype and have an abnormal profile.

tshScer\UAS.cGa under the control of Scer\GAL4cb41 induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs show an increase in size compared to wildtype and have an abnormal profile.

tshScer\UAS.cGa under the control of Scer\GAL4Ser.PGF induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs show an increase in size compared to wildtype and have an abnormal profile.

tshScer\UAS.cGa under the control of Scer\GAL4c329b does not induce ectopic eye formation.

tshScer\UAS.cGa under the control of Scer\GAL4C833 does not induce ectopic eye formation.

tshScer\UAS.cGa under the control of Scer\GAL4MJ33a does not induce ectopic eye formation.

tshScer\UAS.cGa under the control of Scer\GAL4T98 does not induce ectopic eye formation.

Clones expressing tshScer\UAS.cGa, driven by Scer\GAL4αTub84B.PZ, in the eye disc margin or peripodial epithelium induce the overproliferation and columnarization of cells. Some of these clones show a sorting behaviour, with tshdsRNA.Scer\UAS-expressing cells arranging themselves as hollow sacs with their apical sides pointing inwards. Transient tshScer\UAS.cGa expression, driven by Scer\GAL4hs.PB following a heat shock to L2 larvae, results in the development of distinct ectopic clusters of photoreceptor cells in the peripodial epithelium. Such ectopic photoreceptors are seen when tshScer\UAS.cGa is expressed in the margin and the peripodial epithelium of the L3 disc by Scer\GAL4Bx-MS1096 or Scer\GAL4MD705. As adults these flies exhibit ectopic eyes. The Scer\GAL4Bx-MS1096>tshScer\UAS.cGa adults have ventral ectopic eyes are induced that replace ventral head structures and also show small dorsal patches of photoreceptors on the ventral head, which are normally accompanied by a bristle.

Ectopic expression of tshScer\UAS.cGa in the epidermis at 25oC, driven by Scer\GAL469B, causes a homeotic labial to T1 segment transformation, which is visible due to the formation of a denticle T1 "beard" in the labial segment. The maxillary and antennal segments also take on a trunk-like identity, shown by the development of ectopic denticles. Head involution is defective in these mutants and much of the head skeleton is missing. Additionally, these mutants experience a loss of denticles on the trunk.

Changes in cuticle pattern indicate that in tshScer\UAS.cGa; Scer\GAL469B embryos, the labial segment is transformed to T1 and the antennal and maxillary head segments are transformed towards a trunk identity in that they develop denticles and naked cuticle. tshScer\UAS.cGa; Scer\GAL4dpp.blk1 flies have an ectopic eye ventral to each of their eyes, whereas in tshScer\UAS.cGa; Scer\GAL4ey.PH flies the eyes are deformed.

When tshScer\UAS.cGa is driven by Scer\GAL469B, completely suppresses the formation of denticles.

Clones of cells in the eye disc expressing tshScer\UAS.cGa under the control of Scer\GAL4αTub84B.PZ grow poorly in the middle of the disc but can induce large overgrowths at the edge of the disc.

Pharate adults expressing tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 show splitting of the endogenous eye in 8% of cases and sometimes have an ectopic eye at the base of the antenna. 42% of animals expressing tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 arrest and die at the white pupal stage. These animals are about 50% larger than normal. These animals have an extended larval period (which is about 2-3 days longer than normal) and their eye-antennal discs show both overgrowth and distorted morphology. When two copies of tshScer\UAS.cGa are expressed under the control of Scer\GAL4dpp.blk1, the ventral half of the endogenous eye is missing in all flies. Expression of tshScer\UAS.cGa under the control of Scer\GAL4ey.PH results in complete suppression of eye development in 97% of cases. Expression of tshScer\UAS.cGa under the control of Scer\GAL4bi-omb-Gal4 results in absence of the ventral eye in pharate adults and overgrowth of the dorsal eye. The eye disc is enlarged in these animals. Expression of tshScer\UAS.cGa in clones under the control of Scer\GAL4Act5C.PI can suppress photoreceptor development in the eye disc, but only along the ventral margin. Clones in the dorsal margin of the eye disc result in overgrowth. Clones in the internal part of the eye, away from the margins, do not affect eye fate. The frequency of dorsal clones is higher than that of ventral clones. Adults in which clones expressing tshScer\UAS.cGa under the control of Scer\GAL4Act5C.PI have been induced show suppression of the ventral eye or overgrowth of the dorsal eye. Expression of tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 or Scer\GAL4bi-omb-Gal4 results in splitting of the wing pouch. Expression of tshScer\UAS.cGa in clones under the control of Scer\GAL4Act5C.PI results in duplications of antennal segments distal to AN2. Clones in the anteroproximodorsal region of the antennal disc cause ectopic eye formation.

When tshScer\UAS.cGa is driven by Scer\GAL448Y no larvae hatch when reared at 25oC. When reared at 20oC a few escaper larvae are found. In these animals no copper cells are seen in the larval gut.

Expression of the P{UAS-tsh.G}20 insertion under the control of Scer\GAL469B does not affect denticle formation at 25oC.

Somatic clones ectopically expressing tshScer\UAS.cGa under the control of Scer\GAL4Act5C.PP or Scer\GAL4arm.T:Hsim\VP16 in the coxa and trochanter are wild-type. No clones are seen in ventral positions in the leg, however, vesicles consisting of bristleless cuticle are formed. Vesicles of cuticle are also formed in the lumen of the leg resulting in a reduction of femur length and a perturbation at the boundary region in any dorsal-ventral position. When clones are induced in third instar larvae, outgrowths are seen especially in dorsal positions. In distal parts of the femur, clones are observed within which the bristles lacked bracts. After a longer clone induction time, clones located in ventral positions are always abnormal, producing fusion between femur and tibia. Somatic clones ectopically expressing tshScer\UAS.cGa under the control of Scer\GAL4Act5C.PP or Scer\GAL4arm.T:Hsim\VP16 in the leg disc. two or three large clones per disc are seen in the endogenous tsh domain, mostly isolated cells are detected in the distal domain. these cells often sort out from their distal neighbors towards the lumen but remain attached to the epithelium of the disc. When induction time is increased, groups of tsh positive cells extend into the distal domain.

Expression of tshScer\UAS.cGa under the control of Scer\GAL469B results in embryos in which the head segments have trunk identity. These embryos have naked cuticle with almost no ventral denticle belts. The prothoracic beard is not present. Overexpression of tshScer\UAS.cGa in embryos using Scer\GAL4hs.PB between stages 9 and 10 results in a naked cuticle phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

Scer\GAL4dpp.blk1, tshUAS.cGa has eye phenotype, suppressible by wgl-12/wg[+]

Scer\GAL4dpp.blk1, tshUAS.cGa has eye phenotype, suppressible by hthdtl-S142204/hth[+]

NOT suppressed by
Statement
Reference

Scer\GAL469B, tshUAS.cGa has denticle phenotype, non-suppressible by ci94/ci94

Enhancer of
Suppressor of
Statement
Reference

tshUAS.cGa/Scer\GAL469B is a suppressor of embryonic mesothoracic segment & sclerite | ectopic phenotype of spenpoc361

tshUAS.cGa/Scer\GAL469B is a suppressor of embryonic metathoracic segment & sclerite | ectopic phenotype of spenpoc361

NOT Suppressor of
Statement
Reference
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of tshScer\UAS.cGa and araScer\UAS.cGa under the control of Scer\GAL4bi-omb-Gal4 results in enlargement at both the dorsal and ventral margins of the eye disc and adult eye.

Co-expression of tshScer\UAS.cGa and araScer\UAS.cGa under the control of Scer\GAL4Act5C.PI in clones in the eye disc does not affect eye fate.

The eye field is suppressed on both dorsal and ventral margins in eye discs expressing tshScer\UAS.cGa under the control of Scer\GAL4Act5C.PI in clones in the eye disc in a Df(3L)iro-DFM3 background.

Co-expression of tshScer\UAS.cGa and DlScer\UAS.cHa under the control of Scer\GAL4bi-omb-Gal4 results in eye enlargements on both dorsal and ventral margins in both the eye disc and the adult eye.

Ventrally and dorsally located clones in the eye co-expressing tshScer\UAS.cGa and DlScer\UAS.cHa under the control of Scer\GAL4Act5C.PI result in overgrowth.

Co-expression of tshScer\UAS.cGa further enhances the suppression of eye development at the dorsal and ventral margins of the eye disc which is seen in animals expressing DlD.Scer\UAS under the control of Scer\GAL4bi-omb-Gal4.

Clones in the dorsal part of the eye disc which are co-expressing tshScer\UAS.cGa and DlD.Scer\UAS under the control of Scer\GAL4Act5C.PI cause autonomous overgrowth in the posterior margin of the dorsal eye, but photoreceptor differentiation is suppressed within the clone. The overall size of the eye disc is also reduced.

Co-expression of both tshScer\UAS.cGa and SerScer\UAS.cSa under the control of Scer\GAL4bi-omb-Gal4 suppresses eye development in both the dorsal and ventral margins of the eye disc and adult eye. However, despite this suppression of photoreceptor differentiation, the eye disc shows overall enlargement and the adult eye is also enlarged and folded.

Co-expression of tshScer\UAS.cGa partially rescues the suppression of eye development on both the ventral and dorsal margins of the eye that is seen in animals expressing SerBd.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4bi-omb-Gal4.

Clones in the ventral part of the eye that are co-expressing tshScer\UAS.cGa and fngScer\UAS.cKa under the control of Scer\GAL4Act5C.PI show suppression of eye development in the eye disc, whereas clones in the dorsal part of the eye disc that are co-expressing tshScer\UAS.cGa and fngScer\UAS.cKa under the control of Scer\GAL4Act5C.PI induce ectopic eye development.

Co-expression of tshScer\UAS.cGa and CG46146Scer\UAS.cCa under the control of Scer\GAL4Act5C.PI in clones in the eye results in ventral eye suppression and dorsal eye enlargements.

When tshScer\UAS.cGa (driven by Scer\GAL469B) is added to ci94 homozygotes, denticles fail to form.

Clones of cells in the eye disc expressing both hthScer\UAS.T:Avic\GFP and tshScer\UAS.cGa under the control of Scer\GAL4αTub84B.PZ grow well in the middle of the disc and can induce large outgrowths at the edge of the disc.

The split eye phenotype seen in flies expressing tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 is suppressed by hthdtl-S142204/+ and pupal lethality is suppressed to 16% (from 42%). The frequency of the split eye phenotype seen in flies expressing tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 is reduced to 2% by wgl-12 (flies shifted to the restrictive temperature 48-72 hours after egg laying) and pupal lethality is suppressed to 19% (from 42%). Coexpression of both hthScer\UAS.cPa and tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 results in complete eye loss and no antennal eye is produced. Coexpression of both armΔ.Scer\UAS.T:Ivir\HA1 and tshScer\UAS.cGa in clones under the control of Scer\GAL4Act5C.PI suppresses eye development in both the dorsal and ventral domains and both in marginal and internal regions of the eye disc. Coexpression of both armΔ.Scer\UAS.T:Ivir\HA1 and tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 results in complete suppression of the eye. Coexpression of both armΔ.Scer\UAS.T:Ivir\HA1 and tshScer\UAS.cGa under the control of Scer\GAL4bi-omb-Gal4 results in eyes which are reduced in both the dorsal and ventral margins in discs and in adults. Coexpression of both panΔN.Scer\UAS and tshScer\UAS.cGa in clones under the control of Scer\GAL4Act5C.PI does not suppress eye development. Coexpression of both panΔN.Scer\UAS and tshScer\UAS.cGa under the control of Scer\GAL4bi-omb-Gal4 does not result in suppression of eye fate either in the dorsal or ventral margins of the eye. Coexpression of both panΔN.Scer\UAS and tshScer\UAS.cGa under the control of Scer\GAL4ey.PH does not suppress eye development. Coexpression of both sggS9A.Scer\UAS and tshScer\UAS.cGa under the control of Scer\GAL4ey.PH does not suppress eye development. Coexpression of both sggS9A.Scer\UAS and tshScer\UAS.cGa in clones under the control of Scer\GAL4Act5C.PI does not suppress eye development either in the ventral or dorsal margin of the eye. Coexpression of both sggS9A.Scer\UAS and tshScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 does not suppress eye development either in the ventral or dorsal margin of the eye.

When tshScer\UAS.cGa and armS10.Scer\UAS.T:Hsap\MYC are coexpressed in clones in under the control of Scer\GAL4arm.T:Hsim\VP16, very few adults survive. Those that do have no clones.

When expression is driven by Scer\GAL469B the ectopic sclerites of spenpoc361 are completely suppressed.

The lawn of denticles phenotype of pan3 or wgl-17 embryos is replaced by naked cuticle if the embryos are also expressing tshScer\UAS.cGa under the control of Scer\GAL469B. The lawn of denticles phenotype of arm2 embryos derived from arm2 female germ line clones is partly suppressed if the embryos are also expressing tshScer\UAS.cGa under the control of Scer\GAL469B.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Expression of tshScer\UAS.cGa at 22oC, under the control of Scer\GAL469B, rescues the denticle and naked cuticle phenotype of tsh8 mutants and Ti segment identity is restored. However, this expression induces ectopic denticle formation in the head region.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
tshScer\UAS.cGa
tshUAS.cGa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Gallet
Secondary FlyBase IDs
    References (21)