Allele Tn10\tetR^{ey.3.5.T:Hsim\VP16}

General Information
Symbol	Tn10\tetR^{ey.3.5.T:Hsim\VP16}	Species	b. Transposon Tn10
Name		FlyBase ID	FBal0091140
Feature type	allele	Associated gene	Tn10\tetR

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Girard et al., 1998, Bello et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Tn10\tetR^{RHT.T:Hsim\VP16} (Bello et al., 1998)
Nature of the lesion	Statement Reference Construct: Tn10\tetR^{RHT.T:Hsim\VP16} coding sequence expression is driven by a 3.5kb KpnI fragment ey enhancer. (Bello et al., 1998) ey intronic regulator sequences drive expression of Tn10\tetR repressor DNA binding domain (amino acids 1-207) fused to T:Hsim\VP16 transcriptional activation domain (amino acids 363-490). (Girard et al., 1998)
Carried in construct	P{ey-tTA} (Bello et al., 1998, Girard et al., 1998)
Tagged with	T:Hsim\VP16 (Girard et al., 1998, Bello et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	eye, with Antp^{Tn10\tetO.cBa} (Bello et al., 1998)
Detailed Description
	Statement Reference Tn10\tetR^{ey.3.5.T:Hsim\VP16}-mediated expression of Hsap\HMGIY^{MATH20.Tn10\tetO} in In(1)w^m4 flies results in significant derepression of the w gene and a general loss of the variegating phenotype. Tetracycline treatment inhibits Hsap\HMGIY^{MATH20.Tn10\tetO}-induced suppression of PEV. (Girard et al., 1998)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	26801 P{ey-tTA}3, ry⁵⁰⁶
Notes on Origin
Discoverer

Comments
	Used in the tetracyclin (tet)-regulated transactivation system. (Girard et al., 1998)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	ey-tTA (Bello et al., 1998) pSC101\tetR^{ey.3.5.T:Hsim\VP16} tetR::VP16^ey.3.5 tetR::VP16^ey.PG tetR^{ey.3.5.T:Hsim\VP16} Tn10\tetR^{ey.3.5.T:Hsim\VP16}
Name Synonym
Secondary FlyBase IDs
	FBal0088581
References ( 2 )
Research paper	Bello et al., 1998, Development 125(12): 2193--2202 Spatial and temporal targeting of gene expression in Drosophila by means of a tetracycline-dependent transactivator system. [FBrf0103235] Girard et al., 1998, EMBO J. 17(7): 2079--2085 In vivo analysis of scaffold-associated regions in Drosophila: a synthetic high-affinity SAR binding protein suppresses position effect variegation. [FBrf0102177]

Allele Tn10\tetRey.3.5.T:Hsim\VP16

Allele Tn10\tetR^{ey.3.5.T:Hsim\VP16}