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General Information
Symbol
Dmel\stgUAS.cNa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Neufeld
FlyBase ID
FBal0091173
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-stg, UAS-string, UAS-stg.N4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 2.3kb XhoI/XbaI stg cDNA fragment is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of stgScer\UAS.cNa under the control of Scer\GAL4nub.PU leads to a small but significant decrease in wing size, as compared to controls.

Expression of stgScer\UAS.cNa under the control of Scer\GAL4en-e16E does not affect the size of the adult wing posterior compartment, as compared to controls.

Expression of stgScer\UAS.cNa under the control of Scer\GAL4NP7011 produces abdominal segment gaps, segment fusion and polarity reversal phenotypes. In contrast to controls, histoblasts undergo asynchronous division and apoptosis during the early devision phase. Expansion of the histoblast nests is slowed compared with controls but unlike in wild type histoblasts are unable to replace the larval epidermal cells.

Scer\GAL4en.PU-mediated expression reduces the fraction of wing disc cells in G2 phase and concomitantly increases the fraction in G1.

In males expressing stgScer\UAS.cNa under the control of Scer\GAL4bam.T:Hsim\VP16, 97% of germline cysts complete an extra one or two rounds of transit amplification division before differentiating into spermatocytes. 65% of spermatocyte cysts have 32-cells/cyst, and 11% have more than 32-cells/cyst. The length of the cell-cycle is shorter in these mutants, and a decrease in cell size is also observed.

Expression of stgScer\UAS.cNa under the control of Scer\GAL4Act5C.PI in peripodial cell clones drives these cells through extra divisions, creating sharp boundaries between over-proliferating cells and their quiescent neighbours. 3 of 24 stgScer\UAS.cNa-expressing clones contain triangular cells, a shape not observed in wild-type cells.

When stgScer\UAS.cNa expression is driven in follicle cell clones (by Scer\GAL4Act5C.PP), can lead to prolonged mitotic divisions.

When stgScer\UAS.cNa is driven in clones by Scer\GAL4Act5C.PP, cell division is seen to be slightly faster than wild-type.

When stgScer\UAS.cNa is driven by Scer\GAL4bi-omb-Gal4, glial cells in the eye disc undergo excessive proliferation. However these glial cells never cross the axonal boundary.

All cells show uniform entry into mitosis immediately at the beginning of gastrulation in embryos expressing stgScer\UAS.cNa under the control of four maternally provided copies of Scer\GAL4mat.αTub67C.T:Hsim\VP16 in a stgAR2 background. Ventral furrow formation is inhibited in these embryos. The onset of mitosis in embryos expressing stgScer\UAS.cNa under the control of two or three maternally provided copies of Scer\GAL4mat.αTub67C.T:Hsim\VP16 in a stgAR2 background is shifted to a time when the first mitoses normally occur in wild-type embryos. The ventral cells undergoing cell shape changes to form the ventral furrow enter mitosis later than the other cells.

Expression of stgScer\UAS.cNa under the control of Scer\GAL4en-e16E does not result in an overt wing phenotype.

Expression of stgScer\UAS.cNa under the control of Scer\GAL4Act5C.PP in clones in the imaginal discs results in a reduction in the G2 cell population, but cells compensate by lengthening G1 and have only slightly shorter doubling times than normal. The cells are slightly smaller than normal.

Fewer cells are in G2 in wing discs expressing stgScer\UAS.cNa under the control of Scer\GAL4en-e16E than in wild-type wing discs. The number of posterior cells in these wing discs is not significantly different from wild-type. The average duration of cell cycle phases in wing disc cells expressing stgScer\UAS.cNa under the control of Scer\GAL4Act5C.PP is: G1 = 4.8 hours, S = 3.7 hours and G2 = 3.1 hours (wild-type values are G1 = 3.4 hours, S = 4.4 hours and G2 = 4.2 hours). The size of the posterior compartment of wing discs expressing stgScer\UAS.cNa under the control of Scer\GAL4en-e16E is not significantly different from wild-type wing discs.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of stgScer\UAS.cNa does not suppress the decreased wing size induced by the expression of SmoxNIG.2262R under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi).

Co-expression of stgScer\UAS.cNa fails to suppress the wing posterior compartment size reduction seen in flies expressing p115KK100066 under the control of Scer\GAL4en-e16E.

Co-expression of one copy each of mirrGD16318 and stgScer\UAS.cNa under the control of Scer\GAL4ey.PU results in an enlarged eye phenotype not seen when either transgene is expressed alone.

Expression of stgScer\UAS.cNa does not suppress the reduction in wing size and cell number seen when caupScer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4salm.EPv.

Expression of stgScer\UAS.cNa in a striped pattern under the control of Scer\GAL4prd.RG1 in Df(2L)HisC mutant cells results in the cells entering mitosis at M[[15]], whereas cells lacking stg remain arrested. The stg-induced mitosis in Df(2L)HisC embryos is normal up to the metaphase to anaphase transition. During anaphase-b and telophase however, chromosomes begin to lagg and/or chromatin bridges appear in almost all of the cells (>95%). These bridges are eventually resolved during cytokinesis, and the cells enter into interphase of cell cycle 16.

Late third instar wing discs expressing CycEScer\UAS.cLa and stgScer\UAS.cNa under the control of Scer\GAL4sd-SG29.1 have disturbed dorsal-ventral (DV) boundaries compared to controls.

Expression of stgScer\UAS.cNa and CycEScer\UAS.cLa (using the MARCM system under the control of Scer\GAL4tub.PU) fails to rescue the growth defects seen in Df(1)btd-Sp1 leg disc clones generated in second instar larvae.

Co-expression of stgScer\UAS.cNa results in the partial suppression of the curly wing phenotype induced by Scer\GAL4Ser.PGF>Sin3AdsRNA.Scer\UAS.WIZ and restoration of the size of the adult wing blade to that of control wings as well as restoration of cell number.

The delayed differentiation of 16-cell male germline cysts until they are greater than 32-cells/cyst in animals expressing stgScer\UAS.cNa under the control of Scer\GAL4bam.T:Hsim\VP16 is enhanced from 11% to 27% in a bamΔ86/+ mutant background.

Expression of stgScer\UAS.cNa enhances the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU.

When stgScer\UAS.cNa and modScer\UAS.cPa are both driven in clones, an enhancement is seen in the accelerated cell division phenotype seen in stgScer\UAS.cNa, Scer\GAL4Act5C.PP animals.

Coexpression of dltdsRNA.Scer\UAS with stgScer\UAS.cNa, under the control of Scer\GAL4ptc-559.1, has little or no effect on the dltdsRNA.Scer\UAS wing cell phenotype.

Coexpression of stgScer\UAS.cNa completely rescues the second mitotic wave in eye discs expressing ttk69.Scer\UAS under the control of Scer\GAL4GMR.PF. Expression of stgScer\UAS.cNa (under the control of Scer\GAL4αTub84B.PL) in pntΔ88 clones in the eye disc completely rescues the second mitotic wave.

When stgScer\UAS.cNa is driven by Scer\GAL4bi-omb-Gal4, in a eyaunspecified background, glial cells in the optic stalk undergo excessive proliferation. However these glial cells never enter the eye disc.

Coexpression of stgScer\UAS.cNa suppresses the effects of overexpression of trblScer\UAS.cMa under the control of Scer\GAL4en-e16E in imaginal disc cells.

Co-expression of both stgScer\UAS.cNa and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.PP results in shorter doubling times and cells of normal size. Large G1 and small G2 cell populations are seen (as is seen when stgScer\UAS.cNa is expressed under the control of Scer\GAL4Act5C.PP). The areas of clones co-expressing both stgScer\UAS.cNa and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.PP are increased compared to controls (as is seen when Ras85DV12.Scer\UAS is expressed under the control of Scer\GAL4Act5C.PP).

When dmScer\UAS.cZa and stgScer\UAS.cNa are co-expressed via Scer\GAL4Act5C.PP in clones in the wing disc the cells show a 76% reduction in G2 cells (compared to dmScer\UAS.cZa alone). There is an increase of cells in G1 from 5% (dmScer\UAS.cZa alone) to 20%. Doubling time of cells was shortened from 12.9 to 10.6 hours. G1/S progression is promoted and the S and G2 phases of the cell cycle elongated. When dmScer\UAS.cZa and stgScer\UAS.cNa are co-expressed via Scer\GAL4Act5C.PP cells are smaller than for dmScer\UAS.cZa, dmScer\UAS.cZa alone, though the area of the dmScer\UAS.cZa, stgScer\UAS.cNa, Scer\GAL4Act5C.PP clones is larger than that of the dmScer\UAS.cZa, dmScer\UAS.cZa clones, because of reduced doubling time couple with reduced time between cell divisions.

stgScer\UAS.cNa and CycEScer\UAS.cLa bypass the effects of RbfScer\UAS.cDa on cell cycle phasing, cell numbers and cell size when they are both co-expressed with RbfScer\UAS.cDa using Scer\GAL4en-e16E.

Xenogenetic Interactions
Statement
Reference

Expression of stgScer\UAS.cNa partially suppresses the eye phenotypes seen when Mmus\Shisa5Scer\UAS.cGa is expressed under the control of Scer\GAL4dpp.PH.

The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by co-expression of stgScer\UAS.cNa.

Complementation and Rescue Data
Comments

When stgScer\UAS.cNa driven by Scer\GAL4eg-Mz360 or Scer\GAL4en-e16E is added to stg2 homozygotes a rescue of the stg2 phenotype is seen. About 4.2 and 1.8 progeny cells of NB6-4T and NB6-4A respectively are seen when Scer\GAL4eg-Mz360 is the driver and 5.8 and 3.1 progeny cells of NB6-4T and NB6-4A respectively are seen when Scer\GAL4en-e16E is the driver. The surrounding cells are still large as observed in stg2 embryos.

Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
stgScer\UAS.cNa
stgUAS.cNa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Neufeld
Secondary FlyBase IDs
    References (40)