The co-expression of stgScer\UAS.cNa does not suppress the decreased wing size induced by the expression of SmoxNIG.2262R under the control of Scer\GAL4nub.PU (together with Dicer-2, for efficient RNAi).
Expression of stgScer\UAS.cNa in a striped pattern under the control of Scer\GAL4prd.RG1 in Df(2L)HisC mutant cells results in the cells entering mitosis at M[], whereas cells lacking stg remain arrested. The stg-induced mitosis in Df(2L)HisC embryos is normal up to the metaphase to anaphase transition. During anaphase-b and telophase however, chromosomes begin to lagg and/or chromatin bridges appear in almost all of the cells (>95%). These bridges are eventually resolved during cytokinesis, and the cells enter into interphase of cell cycle 16.
Expression of stgScer\UAS.cNa and CycEScer\UAS.cLa (using the MARCM system under the control of Scer\GAL4tub.PU) fails to rescue the growth defects seen in Df(1)btd-Sp1 leg disc clones generated in second instar larvae.
Co-expression of stgScer\UAS.cNa results in the partial suppression of the curly wing phenotype induced by Scer\GAL4Ser.PGF>Sin3AdsRNA.Scer\UAS.WIZ and restoration of the size of the adult wing blade to that of control wings as well as restoration of cell number.
The delayed differentiation of 16-cell male germline cysts until they are greater than 32-cells/cyst in animals expressing stgScer\UAS.cNa under the control of Scer\GAL4bam.T:Hsim\VP16 is enhanced from 11% to 27% in a bamΔ86/+ mutant background.
Expression of stgScer\UAS.cNa enhances the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU.
Coexpression of stgScer\UAS.cNa completely rescues the second mitotic wave in eye discs expressing ttk69.Scer\UAS under the control of Scer\GAL4GMR.PF. Expression of stgScer\UAS.cNa (under the control of Scer\GAL4αTub84B.PL) in pntΔ88 clones in the eye disc completely rescues the second mitotic wave.
When stgScer\UAS.cNa is driven by Scer\GAL4bi-omb-Gal4, in a eyaunspecified background, glial cells in the optic stalk undergo excessive proliferation. However these glial cells never enter the eye disc.
Co-expression of both stgScer\UAS.cNa and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.PP results in shorter doubling times and cells of normal size. Large G1 and small G2 cell populations are seen (as is seen when stgScer\UAS.cNa is expressed under the control of Scer\GAL4Act5C.PP). The areas of clones co-expressing both stgScer\UAS.cNa and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.PP are increased compared to controls (as is seen when Ras85DV12.Scer\UAS is expressed under the control of Scer\GAL4Act5C.PP).
When dmScer\UAS.cZa and stgScer\UAS.cNa are co-expressed via Scer\GAL4Act5C.PP in clones in the wing disc the cells show a 76% reduction in G2 cells (compared to dmScer\UAS.cZa alone). There is an increase of cells in G1 from 5% (dmScer\UAS.cZa alone) to 20%. Doubling time of cells was shortened from 12.9 to 10.6 hours. G1/S progression is promoted and the S and G2 phases of the cell cycle elongated. When dmScer\UAS.cZa and stgScer\UAS.cNa are co-expressed via Scer\GAL4Act5C.PP cells are smaller than for dmScer\UAS.cZa, dmScer\UAS.cZa alone, though the area of the dmScer\UAS.cZa, stgScer\UAS.cNa, Scer\GAL4Act5C.PP clones is larger than that of the dmScer\UAS.cZa, dmScer\UAS.cZa clones, because of reduced doubling time couple with reduced time between cell divisions.
When stgScer\UAS.cNa driven by Scer\GAL4eg-Mz360 or Scer\GAL4en-e16E is added to stg2 homozygotes a rescue of the stg2 phenotype is seen. About 4.2 and 1.8 progeny cells of NB6-4T and NB6-4A respectively are seen when Scer\GAL4eg-Mz360 is the driver and 5.8 and 3.1 progeny cells of NB6-4T and NB6-4A respectively are seen when Scer\GAL4en-e16E is the driver. The surrounding cells are still large as observed in stg2 embryos.