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General Information
Symbol
Dmel\numb796
Species
D. melanogaster
Name
FlyBase ID
FBal0091225
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
nb796
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The GMC-1 cells in numb796 mutant embryos symmetrically divide into two sib cells rather than a sib and an RP2 cell.

In numb796 mutants, the GMC-1 of the RP2/sib lineage symmetrically divides into two sib cells instead of an RP2 and a sib. Penetrance is restricted to ~50% of hemisegments.

GMC-1 of the RP2/sib lineage divides symmetrically to produce two RP2sib cells in mutant embryos.

The Malpighian tubules do not take up their normal positions in numb796 mutant embryos and the tubules lack tip cells.

In mutant embryos Malpighian tubule tip cells are missing except in about 8% embryos, where one or two of the four tubules are allocated normally. However, there is no change in the final number of tubule cells. The distinction between the number of cells in the anterior and posterior tubules is also unaffected.

In about 27% of hemisegments in mutant embryos, a RP2 to RP2sib transformation is seen. This division produces two cells of unequal size, but both cells behave like sib neurons.

27% of mutant hemisegments show an RP2 to RP2sib cell fate transformation. The number of eve-positive CQ neurons is increased. 11% of hemisegments show an RP2 to RP2sib cell fate transformation in numb1/numb796 embryos. 62% of hemisegments show an RP2 to RP2sib cell fate transformation in numb796/Df(2L)30A-C embryos.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

numb796 has RP2 motor neuron phenotype, enhanceable by CycA04335

numb796 has aCC neuron | precursor phenotype, enhanceable by CycA04335

numb796 has pCC neuron | precursor phenotype, enhanceable by CycA04335

Enhancer of
Statement
Reference

numb796 is an enhancer of eo neuron phenotype of fz37/fz30

numb796 is an enhancer of thecogen cell phenotype of fz37/fz30

Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Embryos double mutant for insc22 and HemJ4-48 display hemisegments containing two sib neurons and no RP2 neurons.

In midlos1, numb796 mutants, two ectopic RP2 sib cells are observed with complete penetrance.

Heat-shocked, stage 14 pdm2hs.P; numb796/numb796 embryos have extra RP2 sibs, but not extra RP2 neurons.

Both the daughter cells of the GMC-1 of the RP2/sib lineage adopt an RP2 fate in mam9 numb796 double mutant embryos, indicating that mam is epistatic to numb.

The addition of numb796 to fz37/fz30, leads to pupae that have sense organs composed of four cells with two socket cells but devoid of either sheath or neuronal cells.

In a numb796; Nl1N-ts1 double mutant the sib cell is usually transformed into an RP2 neuron, as in Nl1N-ts1 alone. However, this phenotype is only partially penetrant, as some hemisegments are seen with the numb transformation (RP2 to sib). In about 35% of hemisegments in numb796; CycA04335 double mutants, the GMC of the RP2/sib lineage fails to divide, and assumes the identity of the sib neuron. However, the GMC of the aCC/pCC lineage fails to divide and assumes the identity of the aCC, as in CycA04335 mutants alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

numb796/numb1 is partially rescued by numb2del

Comments

numb+t19.5 efficiently rescues the lethality associated with numb1/numb796 and numb796/Df(2L)30A-C. Adult mechanosensory organ development is almost completely normal in the rescued flies.

numb1/numb796 and numb796/Df(2L)30A-C flies rescued by numb2del show a widespread "double socket" phenotype, in which the shaft cell is transformed to a second socket cell.

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Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (14)