Allele Dmel\ik2⁵

General Information
Symbol	Dmel\ik2⁵	Species	D. melanogaster
Name		FlyBase ID	FBal0091354
Feature type	allele	Associated gene	Dmel\ik2
Also Known As	l(2)38Ea⁶⁶

Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Oshima et al., 2006)
Mutagen	ethyl methanesulfonate (Kozlova et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference This mutation is within the putative kinase domain of the expressed protein. (Kuranaga et al., 2006) Nucleotide substitution: G?A. (Kuranaga et al., 2006) Amino acid replacement: D160N. (Oshima et al., 2006, Kuranaga et al., 2006, Shapiro and Anderson, 2006) The amino acid replacement, caused by a point mutation, is found in the kinase domain of ik2. (Shapiro and Anderson, 2006)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| first instar larval stage \| recessive (Shapiro and Anderson, 2006) lethal \| recessive (Kozlova et al., 1998)
Phenotype Manifest In
	humeral bristle (Shapiro and Anderson, 2006) interommatidial bristle (Shapiro and Anderson, 2006) terminal tracheal cell (Oshima et al., 2006) terminal tracheal cell & filamentous actin (Oshima et al., 2006) terminal tracheal cell & filopodium (Oshima et al., 2006)
Detailed Description
	Statement Reference Apoptosis occurs normally in l(2)38Ea³⁶/l(2)38Ea⁶⁶ and l(2)38Ea⁴⁶/l(2)38Ea⁶⁶ embryos. (Kuranaga et al., 2006) The tracheal branching of l(2)38Ea⁶⁶ embryos occurs normally with apparently normal cell number. At stage 17, the terminal branches show a variety of abnormal morphologies. One class of abnormalities is the duplication of terminal cells. The other classes show persistent filopodia formation in the dorsal side of the terminal cells and the ectopic accumulation of F actin dots, as well as the misorientation and bifurcation of the actin core and the terminal branch. (Oshima et al., 2006) At low temperature and in uncrowded culture conditions, rare escaper ik2⁵ adults (<1%) are observed, but they die shortly after eclosion. These escapers often exhibit abnormal bristles, both the interommatidial and the humeral bristles are affected. At high magnification, the actin footprints along the length of the bristle shaft in ik2⁵ escaper adult eyes appears less organised than those in wild-type bristles. In wild-type, interommatidial bristles are found at alternating vertices, whereas in ik2⁵ mutants these bristles are often duplicated at a single vertex and are misshapen and kinked. (Shapiro and Anderson, 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference ik2⁵ is a suppressor of eye phenotype of rpr^GMR.PH (Kuranaga et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference l(2)38Ea⁶⁶ suppresses the eye ablation phenotype of flies that express rpr^GMR.PH. (Kuranaga et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	5324 y¹ w^*; ik2⁵ dp^ov1 bw¹/CyO, y⁺
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	66 (Oshima et al., 2006) APTX7⁶⁶ (Kuranaga et al., 2006) DmIKKε^D160N (Kuranaga et al., 2006) Ea⁶⁶ (Shapiro and Anderson, 2006) ik2⁵ (Shapiro and Anderson, 2006) ik2⁶⁶ (Matthews, 2007.10.18) l(2)38Ea⁶⁶ (Kuranaga et al., 2006, Oshima, 2006, )
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Kuranaga et al., 2006, Cell 126(3): 583--596 Drosophila IKK-related kinase regulates nonapoptotic function of caspases, via degradation of IAPs. [FBrf0194448] Oshima et al., 2006, Curr. Biol. 16(15): 1531--1537 IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis. [FBrf0193419] Shapiro and Anderson, 2006, Development 133(8): 1467--1475 Drosophila Ik2, a member of the IκB kinase family, is required for mRNA localization during oogenesis. [FBrf0190320] Butler et al., 2001, Genetics 158(4): 1597--1614 Map position and expression of the genes in the 38 region of Drosophila. [FBrf0138405] Kozlova et al., 1998, Genetics 149(3): 1465--1475 Drosophila hormone receptor 38 functions in metamorphosis. A role in adult cuticle formation. [FBrf0102956]
Supplementary material	Oshima, 2006, Curr. Biol. 16(15): [FBrf0199092] Butler, 2001, Genetics 158(4): Supplemental Material 2. Non-P-element mutations considered for the mapping of cytological region 38. [FBrf0139853]
Personal communication to FlyBase	Matthews, 2007.10.18, ik2 synonyms. ik2 synonyms. [FBrf0200203]

Allele Dmel\ik25

Allele Dmel\ik2⁵