FlyBase Allele Report: Dmel\Rbf[UAS.cDa]

Expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^bbg-C96 does not affect wing development. The level of apoptosis in larval wing discs is similar to controls.

Expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^vg.PM results in wing notches. Ectopic wing tissue is not observed, even when the dose and temperature are increased.

Flies expressing Rbf^Scer\UAS.cDa ubiquitously under the control of Scer\GAL4^da.PU are viable and exhibit wing notches. The level of apoptosis in larval wing discs is increased compared to controls, except in the center of the wing pouch.

When Rbf^Scer\UAS.cDa is expressed under the control of Scer\GAL4^ptc.PU the level of proliferation is unchanged from controls.

When Rbf^Scer\UAS.cDa is expressed in 'undead cells' (using BacA\p35^Scer\UAS.cHa) under the control of Scer\GAL4^vg.PM very little wing overgrowth is observed at 25[o]C, although some overgrowth is seen at 29[o]C.

(Milet et al., 2014)

Expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^ey.PH results in a small eye phenotype.

(Datar et al., 2006)

Overexpression of Rbf^Scer\UAS.cDa, under the regulation of Scer\GAL4^sca-537.4 does not alter glial-neuronal expression patterns in the NB6-4t lineage. Ectopic expression of Rbf^Scer\UAS.cDa, under the regulation of Scer\GAL4^sca-537.4 leads to a reduction of neurons in some thoracic segments (45%), but no homeotic transformation.

(Berger et al., 2005)

Expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^vg.PM results in notching of the posterior wing margin. Expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^hs.PB results in atrophic or missing bristles on the adult cuticle.

(Szuplewski et al., 2003)

When Rbf^Scer\UAS.cDa is driven by Scer\GAL4^dpp.blk1, wing phenotypes are seen, the anterior crossvein fails to form and the submarginal cell is reduced in size.

(Weng et al., 2003)

When Rbf^Scer\UAS.cDa is driven by Scer\GAL4^hs.PH during pupal development, abdominal development is significantly delayed. The number of mitoses seen in these abdominal cells is greatly increased, but no abnormalities in centrosome number or spindle morphology are seen.

(Fung et al., 2002)

Rbf^Scer\UAS.cDa driven by Scer\GAL4^ey.PH leads to a small eye phenotype.

(Stevaux et al., 2002)

Expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^ey.PH results in smaller third larval instar eye discs than normal and eyes which are reduced in size. Flies expressing Rbf^Scer\UAS.cDa under the control of Scer\GAL4^hs.2sev have wild-type eyes. Expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^en-e16E results in reduced cell division in the posterior compartment of the wing.

(Tseng and Hariharan, 2002)

Expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^ey.PH results in small abnormally shaped eyes.

(Xin et al., 2002)

Expression of Rbf^Scer\UAS.cDa in clones (driven by Scer\GAL4^Act5C.PP) significantly increases cell doubling times. When driven by Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF no eye phenotype is seen. When driven by Scer\GAL4^ey.PH, severely reduced eyes are seen.

(Datar et al., 2000)

Expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP in clones in the imaginal discs slows all phases of the cell cycle, resulting in large cells with extended doubling times. Co-expression of Rbf^Scer\UAS.cDa and Ras85D^V12.Scer\UAS has an additive effect on cell size.

(Prober and Edgar, 2000)

The average cell doubling time in wing disc cells expressing Rbf^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP is lengthened to 18.5 hours (compared to 12 hours for wild-type cells). The average duration of cell cycle phases in these cells is: G1 = 4.1 hours, S = 9.8 hours and G2 = 4.6 hours (wild-type values are G1 = 3.4 hours, S = 4.4 hours and G2 = 4.2 hours). The size of the posterior compartment of wing discs expressing Rbf^Scer\UAS.cDa under the control of Scer\GAL4^en-e16E is not significantly different from wild-type wing discs.

(Neufeld et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^vg.PM has visible phenotype, suppressible | partially by bsk^NIG.5680R, Scer\GAL4^vg.PM

(Milet et al., 2014)

Rbf^UAS.cDa, Scer\GAL4^vg.PM has visible phenotype, suppressible | partially by hep^r75

(Milet et al., 2014)

Rbf^UAS.cDa, Scer\GAL4^ey.PH has visible phenotype, suppressible by CycD^UAS.cDa, Scer\GAL4^ey.PH

(Datar et al., 2006)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP has decreased occurrence of cell division | somatic clone phenotype, suppressible | partially by CycD^UAS.cDa/Cdk4^UAS.cMa, Scer\GAL4^Act5C.PP

(Datar et al., 2000)

Rbf^UAS.cDa, Scer\GAL4^hs.2sev has abnormal size | adult stage phenotype, suppressible | partially by CycD^UAS.cDa/Cdk4^UAS.cMa, Scer\GAL4^hs.2sev

(Datar et al., 2000)

NOT suppressed by

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by Mmus\Ccnd1^UAS.cDa, Scer\GAL4^ey.PH

(Datar et al., 2006)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP has decreased occurrence of cell division | somatic clone phenotype, non-suppressible by tkv^{Q253D.UAS.cNc}, Scer\GAL4^Act5C.PP

(Martin-Castellanos and Edgar, 2002)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP has decreased occurrence of cell division | somatic clone | cell autonomous | larval stage phenotype, non-suppressible by Ras85D^V12.UAS, Scer\GAL4^Act5C.PP

(Prober and Edgar, 2000)

NOT Enhancer of

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, p53^GUS.PB

(Moon et al., 2008)

Suppressor of

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^hh.PU is a suppressor of increased cell death | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

Rbf^UAS.cDa, Scer\GAL4^ple.PF is a suppressor of abnormal neuroanatomy phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Gehrke et al., 2010)

Rbf^UAS.cDa, Scer\GAL4^Tub.PU is a suppressor of abnormal size | somatic clone phenotype of Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹

(Herz et al., 2010)

Rbf^UAS.cDa, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Rbf^UAS.cDa, Scer\GAL4^repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Rbf^UAS.cDa, Scer\GAL4^Act88F.PD is a suppressor of visible phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Moon et al., 2008, Morris et al., 2006)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^{S202A.T205A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^{T231A.S235A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^{S396A.S404A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

Rbf^UAS.cDa, Scer\GAL4^ey.PH is a suppressor of neoplasia phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, lola^GS88A8, psq^GS88A8

(Ferres-Marco et al., 2006)

Rbf^UAS.cDa, Scer\GAL4^ey.PH is a suppressor of visible phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, lola^GS88A8, psq^GS88A8

(Ferres-Marco et al., 2006)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^elav.PLu is a suppressor of increased cell death | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP is a suppressor of increased cell number | somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP is a suppressor of increased occurrence of cell division | somatic clone | larval stage phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP is a suppressor of increased mortality | somatic clone | pupal stage phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP is a suppressor of neoplasia | somatic clone | adult stage phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

NOT Suppressor of

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, p53^GUS.PB

(Moon et al., 2008)

Other

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc} has increased cell size phenotype

(Martin-Castellanos and Edgar, 2002)

Cdk4^UAS.cMa, CycD^UAS.cDa, Rbf^UAS.cDa, Scer\GAL4^Act5C.PP has increased cell size | somatic clone phenotype

(Datar et al., 2000)

Cdk4^UAS.cMa, Rbf^UAS.cDa, Scer\GAL4^ey.PH has abnormal size phenotype

(Datar et al., 2000)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP has cell cycle | somatic clone phenotype, non-enhanceable by tkv^{Q253D.UAS.cNc}, Scer\GAL4^Act5C.PP

(Martin-Castellanos and Edgar, 2002)

Suppressed by

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^vg.PM has wing phenotype, suppressible | partially by bsk^NIG.5680R, Scer\GAL4^vg.PM

(Milet et al., 2014)

Rbf^UAS.cDa, Scer\GAL4^vg.PM has wing phenotype, suppressible | partially by hep^r75

(Milet et al., 2014)

Rbf^UAS.cDa, Scer\GAL4^ey.PH has eye phenotype, suppressible by CycD^UAS.cDa, Scer\GAL4^ey.PH

(Datar et al., 2006)

Rbf^UAS.cDa, Scer\GAL4^dpp.blk1 has anterior crossvein phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

Rbf^UAS.cDa, Scer\GAL4^dpp.blk1 has submarginal wing cell phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

Rbf^UAS.cDa, Scer\GAL4^ey.PH has eye phenotype, suppressible by CycD^UAS.cDa/Cdk4^UAS.cMa, Scer\GAL4^ey.PH

(Datar et al., 2000)

Rbf^UAS.cDa, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by CycD^UAS.cDa/Cdk4^{D175N.UAS.Tag:MYC}, Scer\GAL4^ey.PH

(Datar et al., 2000)

Rbf^UAS.cDa, Scer\GAL4^hs.2sev has eye phenotype, suppressible | partially by CycD^UAS.cDa/Cdk4^UAS.cMa, Scer\GAL4^hs.2sev

(Datar et al., 2000)

Rbf^UAS.cDa, Scer\GAL4^hs.2sev has eye phenotype, suppressible by Cdk4^UAS.cMa, Scer\GAL4^hs.2sev

(Datar et al., 2000)

Rbf^UAS.cDa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Cdk4^UAS.cMa, Scer\GAL4^GMR.PF

(Datar et al., 2000)

Rbf^UAS.cDa, Scer\GAL4^hs.2sev has eye phenotype, suppressible by CycD^UAS.cDa, Scer\GAL4^hs.2sev

(Datar et al., 2000)

NOT suppressed by

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by Mmus\Ccnd1^UAS.cDa, Scer\GAL4^ey.PH

(Datar et al., 2006)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP has cell cycle | somatic clone phenotype, non-suppressible by tkv^{Q253D.UAS.cNc}, Scer\GAL4^Act5C.PP

(Martin-Castellanos and Edgar, 2002)

Rbf^UAS.cDa, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by Cdk4^{D175N.UAS.Tag:MYC}, Scer\GAL4^ey.PH

(Datar et al., 2000)

Rbf^UAS.cDa, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by Cdk4^UAS.cMa, Scer\GAL4^ey.PH

(Datar et al., 2000)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP has mitotic cell cycle | somatic clone phenotype, non-suppressible | somatic clone by Ras85D^V12.UAS, Scer\GAL4^Act5C.PP

(Prober and Edgar, 2000)

NOT Enhancer of

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, p53^GUS.PB

(Moon et al., 2008)

Suppressor of

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^hh.PU is a suppressor of wing disc posterior compartment | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

Rbf^UAS.cDa, Scer\GAL4^ple.PF is a suppressor of dopaminergic neuron phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Gehrke et al., 2010)

Rbf^UAS.cDa, Scer\GAL4^Tub.PU is a suppressor of eye | somatic clone phenotype of Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹

(Herz et al., 2010)

Rbf^UAS.cDa, Scer\GAL4^Tub.PU is a suppressor of adult head | somatic clone phenotype of Delta^UAS.cUa, Scer\GAL4^Tub.PU, Utx¹

(Herz et al., 2010)

Rbf^UAS.cDa, Scer\GAL4^repo.PU is a suppressor of glial cell | increased number | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Rbf^UAS.cDa, Scer\GAL4^repo.PU is a suppressor of larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Rbf^UAS.cDa, Scer\GAL4^Act88F.PD is a suppressor of wing pouch phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Moon et al., 2008)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{S202A.T205A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{T231A.S235A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{S396A.S404A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

Rbf^UAS.cDa, Scer\GAL4^ey.PH is a suppressor of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH, lola^GS88A8, psq^GS88A8

(Ferres-Marco et al., 2006)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^E14.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^elav.PLu is a suppressor of adult brain | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^elav.PLu is a suppressor of lamina | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

Scer\GAL4^GMR.PF, dap^UAS.cdNa/Rbf^UAS.cDa is a suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Rheb^AV4, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

dap^UAS.cdNa, Scer\GAL4^GMR.PF, Rbf^UAS.cDa is a suppressor of eye | conditional phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

Rbf^UAS.cDa, Scer\GAL4^Act88F.PD is a suppressor of wing blade phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Morris et al., 2006)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP is a suppressor of cell cycle | somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP is a suppressor of wing vein | ectopic | somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP is a suppressor of wing vein | somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

Rbf^UAS.cDa, Scer\GAL4^Act5C.PP is a suppressor of wing | somatic clone phenotype of Scer\GAL4^Act5C.PP, tkv^{Q253D.UAS.cNc}

(Martin-Castellanos and Edgar, 2002)

NOT Suppressor of

Statement

Reference

Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, p53^GUS.PB

(Moon et al., 2008)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

Other

Statement

Reference

Cdk4^UAS.cMa, Rbf^UAS.cDa, Scer\GAL4^ey.PH has eye phenotype

(Datar et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of bsk^NIG.5680R partially suppresses the wing phenotypes seen when Rbf^Scer\UAS.cDa is expressed under the control of Scer\GAL4^vg.PM.

A hep^r75 mutant background partially suppresses the wing phenotypes seen when Rbf^Scer\UAS.cDa is expressed under the control of Scer\GAL4^vg.PM.

(Milet et al., 2014)

Overexpression of Rbf^Scer\UAS.cDa suppresses the dopaminergic neuronal phenotypes in animals expressing Lrrk^{I1915T.Scer\UAS} under the control of Scer\GAL4^ple.PF.

(Gehrke et al., 2010)

Co-expression of Rbf^Scer\UAS.cDa suppresses the strong overgrowth phenotype seen when Dl^Scer\UAS.cUa is overexpressed in Utx¹ mosaic eyes.

(Herz et al., 2010)

Expression of Rbf^Scer\UAS.cDa suppresses the glial neoplasia seen in third instar larvae when btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU. The gross neural morphogenesis defects are also suppressed.

(Read et al., 2009)

The wing phenotype of Scer\GAL4^Act88F.PD, E2f^Scer\UAS.cNa is suppressed by co-expression of Rbf^Scer\UAS.cDa.

The eye phenotype resulting from the overexpression of p53^GUS.PB via Scer\GAL4^GMR.PF is unaffected by the co-expression of Rbf^Scer\UAS.cDa.

(Moon et al., 2008)

Co-expression of CycD^Scer\UAS.cDa suppresses the small eye phenotype caused by expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^ey.PH.

(Datar et al., 2006)

The eye tumour phenotype of flies expressing Dl^Scer\UAS.cDa under the control of Scer\GAL4^ey.PH and also carrying the P{GSV1}lola^GS88A8 insertion (which results in the lola^GS88A8 and psq^GS88A8 alleles being expressed under the control of Scer\GAL4^ey.PH) is suppressed by co-expression of Rbf^Scer\UAS.cDa.

(Ferres-Marco et al., 2006)

The addition of tkv^{Q253D.Scer\UAS.cNc} to Rbf^Scer\UAS.cDa (driven by Scer\GAL4^Act5C.PP) has no effect on the reduced proliferation phenotype seen in Rbf^Scer\UAS.cDa, Scer\GAL4^Act5C.PP alone, though cells are significantly larger than Rbf^Scer\UAS.cDa, Scer\GAL4^Act5C.PP cells.

(Martin-Castellanos and Edgar, 2002)

Co-expression of Rbf^Scer\UAS.cDa, Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa in clones (driven by Scer\GAL4^Act5C.PP) produces cells that cycle more rapidly than cells expressing Rbf^Scer\UAS.cDa alone. Clones have fewer cells than wild-type controls, but they encompass substantially more area. Co-expression of Rbf^Scer\UAS.cDa and Cdk4^Scer\UAS.cMa when driven by Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF no eye phenotype is seen. When driven by Scer\GAL4^ey.PH, severely reduced eyes are seen. Co-expression of Rbf^Scer\UAS.cDa and CycD^Scer\UAS.cDa when driven by Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF no eye phenotype is seen. When driven by Scer\GAL4^ey.PH, slightly reduced eyes are seen. This phenotype is slightly enhanced by the addition of Cdk4^{D175N.Scer\UAS.T:Hsap\MYC}. Co-expression of Rbf^Scer\UAS.cDa, Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa, when driven by Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF leads to a slight eye phenotype. When driven by Scer\GAL4^ey.PH a slightly reduced eye phenotype is seen. The addition of Cdk4^{D175N.Scer\UAS.T:Hsap\MYC} to Rbf^Scer\UAS.cDa flies (when driven by Scer\GAL4^ey.PH, Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF) has no effect on the eye phenotype.

(Datar et al., 2000)

The inhibitory effect on cell cycle rates of Rbf^Scer\UAS.cDa expressed under the control of Scer\GAL4^Act5C.PP in clones is not suppressed by co-expression of Ras85D^V12.Scer\UAS.

(Prober and Edgar, 2000)

The effects caused by Rbf^Scer\UAS.cDa on cell cycle phasing, cell numbers and cell size are dominant to effects caused by E2f^Scer\UAS.cNa when they are co-expressed using Scer\GAL4^en-e16E. stg^Scer\UAS.cNa and CycE^Scer\UAS.cLa bypass the effects of Rbf^Scer\UAS.cDa on cell cycle phasing, cell numbers and cell size when they are both co-expressed with Rbf^Scer\UAS.cDa using Scer\GAL4^en-e16E. 77 hour old clones expressing Rbf^Scer\UAS.cDa and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E (induced early in wing disc development) are roughly half the size of control clones.

(Neufeld et al., 1998)

Xenogenetic Interactions

Statement

Reference

Co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa substantially suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^{T231A.S235A.Scer\UAS}-induced eye phenotype.

Co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa substantially suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^{S202A.T205A.Scer\UAS}-induced eye phenotype.

Co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa substantially suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^Scer\UAS.cWa-induced eye phenotype.

(Steinhilb et al., 2007)

Co-expression of Mmus\Ccnd1^Scer\UAS.cDa does not suppress the small eye phenotype caused by expression of Rbf^Scer\UAS.cDa under the control of Scer\GAL4^ey.PH.

(Datar et al., 2006)

The rough-eye phenotype observed in animals expressing Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

Co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa in adult brains expressing Hsap\MAPT^{R406W.Scer\UAS} under the control of Scer\GAL4^elav.PLu significantly reduces the number of dying cells observed. Vacuolar degeneration of the lamina is rescued in these animals.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of Rheb^AV4. This enhancement is blocked by co-expressing dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^Scer\UAS.cWa under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

The rough-eye phenotype observed in animals expressing Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} in the eye under the control of Scer\GAL4^GMR.PF is not modified by co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

(Khurana et al., 2006)

Complementation and Rescue Data

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Mutant

Wild-type

Stocks (2)

Bloomington

50746

w^*; P{UAS-Rbf.D}II

50747

w^*; P{UAS-Rbf.D}III

Notes on Origin

Discoverer

N. Dyson

(Neufeld et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rbf^Scer\UAS.cDa

Rbf^UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Dyson

(Neufeld et al., 1998)

Secondary FlyBase IDs

References (33)

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