Allele Dmel\Ras85D^V12.hs

General Information
Symbol	Dmel\Ras85D^V12.hs	Species	D. melanogaster
Name		FlyBase ID	FBal0091660
Feature type	allele	Associated gene	Dmel\Ras85D

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Miller and Cagan, 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Ras85D^V12 coding region expression is driven by a heat shock promoter. (Miller and Cagan, 1998)
Carried in construct	P{hsRas1.V12} (Hayashi and Saigo, 2001, Martin-Blanco et al., 1999, Spencer et al., 1998, Rusconi et al., 2004, Sawamoto et al., 1998, Miller and Cagan, 1998, Tong et al., 2007, Gorski et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	visible \| heat sensitive (Martin-Blanco et al., 1999)
Phenotype Manifest In
	cone cell \| heat sensitive (Sawamoto et al., 1998) eye disc \| heat sensitive (Hayashi and Saigo, 2001) ommatidium (Rusconi et al., 2004) secondary pigment cell \| heat sensitive (Sawamoto et al., 1998) secondary pigment cell \| precursor (Miller and Cagan, 1998) tertiary pigment cell \| heat sensitive (Sawamoto et al., 1998) tertiary pigment cell \| precursor (Miller and Cagan, 1998) wing vein \| heat sensitive (Martin-Blanco et al., 1999)
Detailed Description
	Statement Reference Ras85D[V12.hs] heterozygous flies maintained at 25[o]C exhibit normal life spans and locomotor activity. Overexpression of Ras85D[V12.hs] does not affect superoxide production. (Tong et al., 2007) Expression of Ras85D^V12.hs using heat shock at 24 hours after puparium formation results in extra lattice cells in the retina; there are an average of 18.5 +/- 0.3 cells per ommatidial unit (compared to 12 cells per ommatidial unit in wild-type animals). (Rusconi et al., 2004) Heat shock expression of Ras85D^V12.hs can lead to a morphogenetic furrow arrest phenotype in the developing eye. (Hayashi and Saigo, 2001) Expression of Ras85D^V12.hs during late larval development using heat shock results in flies that show a loss of wing veins. (Martin-Blanco et al., 1999) Ablation of cone cells in four adjacent developing ommatidia at 20 hrs APF causes death of interommatidial precursor cells in wild type. This IPC death is blocked by N^fa-g62 or Ras85D^V12.hs. (Miller and Cagan, 1998) Transient expression by heat shock during early pupal development (0-5.5 hr APF) causes an increase in cone-like cells. Heat shock during later pupal development (20-40 hr APF) has no effect on cone cells but causes an increase in the number of secondary and tertiary pigment cells. (Sawamoto et al., 1998)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Ras85D^V12.hs has ommatidium phenotype, enhanceable by klu[+]/klu^unspecified (Rusconi et al., 2004)
Suppressor of
Statement Reference Ras85D^V12.hs is a suppressor of cone cell phenotype of aos^GMR.PS (Sawamoto et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference klu^unspecified/+ enhances the extra cell phenotype seen in the retina of animals expressing Ras85D^V12.hs using heat shock at 24 hours after puparium formation; the double mutant animals have an average of 20.03 +/- 0.2 cells per ommatidial unit (compared to an average of 18.5 +/- 0.3 cells per ommatidial unit in animals expressing Ras85D^V12.hs at the same time in a klu⁺ background). (Rusconi et al., 2004) Flies carrying both Ras85D^V12.hs and argos^GMR.PS show a normal number of cone cells in most ommatidia. (Sawamoto et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	N. Perrimon

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Dras1^Val12 (Spencer et al., 1998) Ras85D^V12.hs ras^v12 (Gorski et al., 2000)
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Tong et al., 2007, Nat. Genet. 39(4): 476--485 Life extension through neurofibromin mitochondrial regulation and antioxidant therapy for neurofibromatosis-1 in Drosophila melanogaster. [FBrf0200172] Rusconi et al., 2004, Mech. Dev. 121(6): 537--546 klumpfuss regulates cell death in the Drosophila retina. [FBrf0179423] Hayashi and Saigo, 2001, Mech. Dev. 108(1-2): 13--27 Diversification of cell types in the Drosophila eye by differential expression of prepattern genes. [FBrf0139741] Gorski et al., 2000, Cell Death Differ. 7(10): 1011--1013 Delta and notch promote correct localization of irreC-rst. [FBrf0136820] Martin-Blanco et al., 1999, Development 126(24): 5739--5747 A temporal switch in DER signaling controls the specification and differentiation of veins and interveins in the Drosophila wing. [FBrf0111967] Miller and Cagan, 1998, Development 125(12): 2327--2335 Local induction of patterning and programmed cell death in the developing Drosophila retina. [FBrf0103328] Sawamoto et al., 1998, Cell Death Differ. 5(4): 262--270 Argos induces programmed cell death in the developing Drosophila eye by inhibition of the Ras pathway. [FBrf0103030] Spencer et al., 1998, Development 125(23): 4777--4790 Regulation of EGF receptor signaling establishes pattern across the developing Drosophila retina. [FBrf0107294]

Allele Dmel\Ras85DV12.hs

Allele Dmel\Ras85D^V12.hs